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Nishiguchi, Tomoki,Jiang, Zedong,Ueno, Mikinori,Takeshita, Satoshi,Cho, Kichul,Roh, Seong Woon,Kang, Kyong-Hwa,Yamaguchi, Kenichi,Kim, Daekyung,Oda, Tatsuya The Korean Society of Phycology 2014 ALGAE Vol.29 No.3
Polysaccharides prepared from marine algae sometimes contain contaminants such as polyphenols and endotoxins that may mislead their bona fide biological activities. In this study, we examined bioactive contaminants in commercially available fucoindan from Fucus vesiculosus, along with ascophyllan and fucoidan from Ascophyllum nodosum. F. vesiculosus fucoidan inhibited the growth of Vibrio alginolyticus in a concentration-dependent manner ($0-1,000{\mu}g\;mL^{-1}$). However, the antibacterial activity of the fucoidan significantly reduced after methanol-extraction, and the methanol-extract showed a potent antibacterial activity. The extract also showed cytotoxicity to RAW264.7 and U937 cells, and induced apoptotic nuclear morphological changes in U937 cells. These results suggest that the antibacterial activity of the fucoidan is partly due to the methanol-extractable contaminants that can also contribute to the cytotoxicity on RAW264.7 and U937 cells. On the other hand, the activities to induce secretion of nitric oxide and tumor necrosis factor-${\alpha}$ from RAW264.7 cells were observed in the fucoidan even after methanol extraction, and the extract had no such activities. Our observations suggest that commercially available fucoidan should be purified prior to biochemical use.
Tomoki Nishiguchi,Zedong Jiang,Mikinori Ueno,Satoshi Takeshita,Kichul Cho,노성운,Kyong-Hwa Kang,Kenichi Yamaguchi,Daekyung Kim,Tatsuya Oda 한국조류학회I 2014 ALGAE Vol.29 No.3
Polysaccharides prepared from marine algae sometimes contain contaminants such as polyphenols and endotoxins that may mislead their bona fide biological activities. In this study, we examined bioactive contaminants in commercially available fucoindan from Fucus vesiculosus, along with ascophyllan and fucoidan from Ascophyllum nodosum. F. vesiculosus fucoidan inhibited the growth of Vibrio alginolyticus in a concentration-dependent manner (0-1,000 μg mL-1). However, the antibacterial activity of the fucoidan significantly reduced after methanol-extraction, and the methanol-extract showed a potent antibacterial activity. The extract also showed cytotoxicity to RAW264.7 and U937 cells, and induced apoptotic nuclear morphological changes in U937 cells. These results suggest that the antibacterial activity of the fucoidan is partly due to the methanol-extractable contaminants that can also contribute to the cytotoxicity on RAW264.7 and U937 cells. On the other hand, the activities to induce secretion of nitric oxide and tumor necrosis factor-α from RAW264.7 cells were observed in the fucoidan even after methanol extraction, and the extract had no such activities. Our observations suggest that commercially available fucoidan should be purified prior to biochemical use.
Expression of Human Lactoferrin Gene in Transgenic Rice(Oryza sativa L.)
이효연,Yong-Eok Lee,Seong-Eun Oh,Satoshi Nishiguchi,Key-Zung Riu,송인자,박신영,Jin-Hyoung Lee,Il-Gi Kim,서석철,Seong-Lyul Rhim,임평옥 한국식물생명공학회 2007 JOURNAL OF PLANT BIOTECHNOLOGY Vol.34 No.2
Lactoferrin is an 80-kDa iron-binding glycoprotein known to exert many biological activities, such as facilitating iron absorption and having antimicrobial and anti-inflammatory effects. Rice can be a useful target for edible food plants to introduce human lactoferrin, because it has lower allergenicity and is likely to be safer than microorganisms or transgenic animals. A cDNA fragment encoding human lactoferrin (HLF) driven by the maize polyubiquitin promoter, along with herbicide resistance gene (bar) driven by CaMV 35S promoter, was introduced into rice (Oryza sativa L. cv. Dong Jin) using the Agrobacterium -mediated transformation system. Putative transformants were initially selected on the medium containing bialaphos. The stable integration of the bar and HLF genes into transgenic rice plants was further confirmed through polymerase chain reaction (PCR) and Southern blot analyses. The expression of the full length HLF protein from various tissues such as grains and young leaves of transgenic rice was verified by Western blot analysis. Analysis of progeny also demonstrated that introduced genes were stably inherited to the next generation at the Mendelian fashion.
Expression of Human Lactoferrin Gene in Transgenic Rice (Oryza sativa L.)
Lee, Yong-Eok,Oh, Seong-Eun,Nishiguchi, Satoshi,Riu, Key-Zung,Song, In-Ja,Park, Shin-Young,Lee, Jin-Hyoung,Kim, Il-Gi,Suh, Suk-Chul,Rhim, Seong-Lyul,Lim, Pyung-Ok,Lee, Hyo-Yeon The Korean Society of Plant Biotechnology 2007 식물생명공학회지 Vol.34 No.2
Lactoferrin is an 80-kDa iron-binding glycoprotein known to exert many biological activities, such as facilitating iron absorption and having antimicrobial and anti-inflammatory effects. Rice can be a useful target for edible food plants to introduce human lactoferrin, because it has lower allergenicity and is likely to be safer than microorganisms or transgenic animals. A cDNA fragment encoding human lactoferrin (HLF) driven by the maize polyubiquitin promoter, along with herbicide resistance gene (bar) driven by CaMV 35S promoter, was introduced into rice (Oryza sativa L. cv. Dong Jin) using the Agrobacterium -mediated transformation system. Putative transformants were initially selected on the medium containing bialaphos. The stable integration of the bar and HLF genes into transgenic rice plants was further confirmed through polymerase chain reaction (PCR) and Southern blot analyses. The expression of the full length HLF protein from various tissues such as grains and young leaves of transgenic rice was verified by Western blot analysis. Analysis of progeny also demonstrated that introduced genes were stably inherited to the next generation at the Mendelian fashion.
Mallard Detection using Microphone Array and Delay-and-Sum Beamforming
Masumi Hashimoto,Hirokazu Madokoro,Kanji Watanabe,Masayuki Nishiguchi,Satoshi Yamamoto,Hanwool Woo,Kazuhito Sato 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10
This paper presents a method of estimating the direction and position of mallards from voice information using arrayed microphones combined with delay-and-sum beamforming. Rice farming using mallards saves labor because mallards work instead of farmers. Nevertheless, the number of mallards declines when they are preyed upon by natural enemies such as crows, kites, and weasels. We consider that efficient management can be achieved from locating and identifying the locations of mallards and natural enemies using voice information that can be widely sensed in a paddy field. For this study, we developed a prototype system with 32 microphones installed as an array installed on our originally designed and assembled mount. We obtained benchmark sound datasets in an outdoor environment that comprises 18 grid points of 40 m width and 30 m depth. For direction estimation, no detection angle error occurred at 9 points. Estimation errors were increased at sharp-angle positions from the microphone array. For position estimation, our method correctly estimated 8 points.
Ganesan, Markkandan,Han, Yun-Jeong,Bae, Tae-Woong,Hwang, Ok-Jin,Chandrasekkhar, Thummala,Shin, Ah-Young,Goh, Chang-Hyo,Nishiguchi, Satoshi,Song, In-Ja,Lee, Hyo-Yeon,Kim, Jeong-Il,Song, Pill-Soon Springer-Verlag 2012 Planta Vol.236 No.4
<P>Phytochrome A (phyA) in higher plants is known to function as a far-red/shade light-sensing photoreceptor in suppressing shade avoidance responses (SARs) to shade stress. In this paper, the Avena PHYA gene was introduced into creeping bentgrass (Agrostis stolonifera L.) and zoysiagrass (Zoysia japonica Steud.) to improve turf quality by suppressing the SARs. In addition to wild-type PHYA, a hyperactive mutant gene (S599A-PHYA), in which a phosphorylation site involved in light-signal attenuation was removed, was also transformed into the turfgrasses. Phenotypic traits of the transgenic plants were compared to assess the suppression of SARs under a simulated shade condition and outdoor field conditions after three growth seasons. Under the shade condition, the S599A-PhyA transgenic creeping bentgrass plants showed shade avoidance-suppressing phenotypes with a 45?% shorter leaf lengths, 24?% shorter internode lengths, and twofold increases in chlorophyll concentrations when compared with control plants. Transgenic zoysiagrass plants overexpressing S599A-PHYA also showed shade-tolerant phenotypes under the shade condition with reductions in leaf length (15?%), internode length (30?%), leaf length/width ratio (19?%) and leaf area (22?%), as well as increases in chlorophyll contents (19?%) and runner lengths (30?%) compared to control plants. The phenotypes of transgenic zoysiagrass were also investigated in dense field habitats, and the transgenic turfgrass exhibited shade-tolerant phenotypes similar to those observed under laboratory shade conditions. Therefore, the present study suggests that the hyperactive phyA is effective for the development of shade-tolerant plants, and that the shade tolerance nature is sustained under field conditions.</P>