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Park, Sang-Un,Kong, Hyunjung,Shin, Dong-Jin,Bae, Chang-Hwan,Lee, Sheong-Chun,Bae, Chang-Hyu,Rha, Eui-Shik,Kim, Haeng-Hoon Royal Veterinary College 2014 Cryo letters Vol.35 No.2
<P>A solution-based vitrification protocol is a process of sequentially changing-solutions from which both influx of cryoprotectants (loading) and efflux of water (dehydration) were accomplished before cryo-exposure. Hence, we need to properly control the concentration /composition of the cryoprotectant solutions.</P>
중부지역 옥수수 파종기에 따른 생육단계 및 수량성 변화
서종호, 문중경, 정건호, 서민정, 구자환, 박장환, 배환희, 신성휴, 권영업 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Maize hybrids were planted on April 1, April 25, May 20 and June 15 in 2012, 2013 at the experimental paddy and upland fields in Suwon city , respectively to know changes of growth stage and yield according to different planting time. Maize planting on April 1 was not recommended because total growing days at planting on April 1 was not different with that at planting on April 25 due to many days from planting to emergence over 20 days at planting on April 1. Total growing days were not much different among three planting time after April 25 because days from silking to maturity was lengthened though days from planting to silking was shortened at later planting time. Stalk weight was decreased as planting time was delayed, particularly at planting times after May 20 at paddy field by excess water injury during early growth stage. But, grain yield was not low as in May and June. It is needed to introduce early maturity maize variety as Yanganok at late planting for higher grain yield and enough time for soil preparation for next winter crops
중이온 빔 조사에 의한 담배의 돌연변이 유도와 내염성 식물의 선발
배창휴,Abe, Tomoko,민경수,김동철,정재성,이춘환,임용표,이효연 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-
중이온 빔을 이용한 방사선 돌연변이의 기초자료를 얻고자 담배의 수분·수정 직후의 원배에 100 Gy의 중이온빔을 조사하여 M_1세대의 종자를 얻었다. NaCI 2.0과 2.5%를 포함된 MS배지에 M_1 세대의 종자를 파종하여 17개통의 NaCI 저항성 식물체를 선발하였다. 저항성 식물체는 BY-4품종에 ^14N빔을 조사한 처리구에서만 관찰되었다. M_1 세대의 저항성 식물체의 경우 영양생장기간 중에는 외형적 변이가 관찰되지 않았으나 생식생장기의 화기에 있어서는 다양한 변이가 관찰되었다. 특히 암술 수술의 길이의 변화, 꽃잎의 변화, 불완전 화분립, 줄기당 꽃의 착생수 감소 등의 변이가 발생하였다. M_1 세대에서 17개의 NaCI저항성 계통 중에서 8계통은 M_2 세대에도 저항성형질이 발현되었고, 또한 M_2 세대의 3계통은 Mannitol이 포함된 배지에서 저항성을 보여주었다. Tobacco proembryos were irradiated with 100 Gy of heavy-ion beams (^14N, ^20Ne : 135 Mev/u) after 24 to 96 hours of pollination as a mutagen and screened M_1 generation for morphological mutants and salt-tolerant plants. Morphological and physilolgical characteristics of the salt-tolerant plants derived from the irradiated proembryo are discussed in this report. Mutants irradiated porembryos with the beams after pollination produced various kinds of morphological variation. A total of 17 salt-tolerant plants were selected from tobacco cultivar (BY-4) by treatment with ^14N beam. Shapes of filament and pollen grain of most salt-tolerant plants were abnormal compared with non-irradiated wild type, and seeds weight and fertility obviously decreased. The germination rates of the several M_2 lines on the saline and the mannitol condition were higher than that of wild type.
Ryu, Jai Hyunk,Seo, Kyoung Sun,Choi, Gab Lim,Rha, Eui Shik,Lee, Sheong Chun,Choi, Seong Kyu,Kang, Si-Yong,Bae, Chang-Hyu The Plant Resources Society of Korea 2012 한국자원식물학회지 Vol.25 No.6
Dandelion has been widely utilized for medicinal and edible purposes. This research was conducted to evaluate the effect of supplemental LED (light-emitting diode) light on germination, growth characteristics and anthocyanin content of dandelion (Taraxacum officinale) seedling using LED blue (460 nm), red (660 nm, R), blue + red (B:R=6:4) and fluorescent lamp light treatment. By LED illumination to T. officinale seed germination speed was delayed, and germination rate was the highest in the fluorescent light. The growth characteristics (plant height, number of leaves, root length and fresh weight) were greatly influenced by supplemental LED light compared with control treatment, and the growth promotion was the most effective in the red LED illumination. After 60 days of red and mixed LED light treatments, anthocyanin content of dandelion plants was significantly changed. The anthocyanin content was increased by 12~19 mg/100 g under the red LED and the mixed light conditions compared with the control and the blue LED. Results indicate that illumination with red and mix LEDs, compared with other light treatments, is beneficial for promotion of growth and anthocyanin content in dandelion.
Bae, Chang-Hyu,Lee, Young-Ill,Lim, Yong-Pyo,Seo, Yong-Won,Lee, Do-Jin,Yang, Deuk-Chun,Lee, Hyo-Yeon The Korean Society of Plant Biotechnology 2002 Plant molecular biology and biotechnology research Vol.4 No.3
Herbicide tolerant rice (Oryza sativa L. cv. Ilpumbyeo) cell lines were selected from $\gamma$-ray-irradiated anther-derived cell cultures. The anther-derived cell clusters were small (300 to 400 ${\mu}{\textrm}{m}$ in diameter) and uniform ones that were screened by miracloth filtering. The cell suspensions were very efficient to plate one layer onto agar medium and to screen target cell lines. Herbicide tolerant cell lines were selected by 5 mg/L cyhalofop butyl (CHB) treatment by using the small cell suspensions on agar N6 medium containing 1 mg/L 2,4-D and 0.2 mg/L kinetin. Of the cell lines, one line (CHB-1) showed stable tolerance at 10 mg/L concentration after 6-month culture without herbicide suspension. Growth stability of CHB-1 was similar to that of control cell line on 10 mg/L CHB containing medium. In this experiment we established herbicide tolerant cell line selection system by using anther-derived uniform-cell suspensions with $\gamma$-ray-irradiation.