Replication origins oriGNAI3 and oriB of the mammalian AMPD2 Locus nested in a region of straight DNA flanked by intrinsically bent DNA sites

( Valerio Americo Balani ),( Quirino Alves De Lima Neto ),( Karen Izumi Tareda ),( Fabricia Gimenes ),( Adriana Fiorini ),( Michelle Debatisse ),( Maria Aparecida Fernandez ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.11

The aim of this work was to determine whether intrinsically bent DNA sites are present at, or close to, the mammalian replication origins oriGNAI3 and oriB in the Chinese hamster AMPD2 locus. Using an electrophoretic mobility shift assay and in silico analysis, we located four intrinsically bent DNA sites (b1 to b4) in a fragment that contains the oriGNAI3 and one site (b5) proximal to oriB. The helical parameters show that each bent DNA site is curved in a left-handed superhelical writhe. A 2D projection of 3D fragment trajectories revealed that oriGNAI3 is located in a relatively straight segment flanked by bent sites b1 and b2, which map in previously identified Scaffold/Matrix Attachment Region. Sites b3 and b4 are located approximately 2 kb downstream and force the fragment into a strong closed loop structure. The b5 site is also located in an S/MAR that is found just downstream of oriB. [BMB reports 2010; 43(11): 744-749]

디지털 노동 플랫폼에 관한 유럽의 법적 프레임워크

Valerio De Stefano,Antonio Aloisi 한국노동연구원 2020 국제노동브리프 Vol.18 No.5

Bone changes in the mandibular incisors after orthodontic correction of dental crowding without extraction: A cone-beam computed tomographic evaluation

Valerio, Claudia Scigliano,Cardoso, Claudia Assuncao e Alves,Arauujo, Eustaquio Afonso,Zenobio, Elton Goncalves,Manzi, Flavio Ricardo Korean Academy of Oral and Maxillofacial Radiology 2021 Imaging Science in Dentistry Vol.51 No.2

Purpose: This study aimed to evaluate changes of the alveolar bone and interdental bone septum of the mandibular incisors through cone-beam computed tomography (CBCT) after orthodontic treatment of mandibular dental crowding without dental extraction. Materials and Methods: The sample consisted of 64 CBCT images(32 pre-treatment and 32 post-treatment) from 32 adult patients with class I malocclusion and an average age of 23.0±3.9 years. The width and height of the alveolar bone and interdental septum, the distance between the cementoenamel junction (CEJ) and the facial and lingual bone crests, and the inclination of the mandibular incisors were measured. Results: The distance between the CEJ and the marginal bone crest on the facial side increased significantly (P<0.05). An increased distance between the CEJ and the bone crest on the facial and lingual sides showed a correlation with the irregularity index (P<0.05); however, no significant association was observed with increasing mandibular incisor inclination (P>0.05). The change in the distance between the CEJ and the marginal bone crest on the facial side was correlated significantly with bone septum height(P<0.05). Conclusion: Bone dehiscence developed during the treatment of crowding without extraction only on the incisors' facial side. Increasing proclination of the mandibular incisor was not correlated with bone dehiscence. The degree of dental crowding assessed through the irregularity index was associated with the risk of developing bone dehiscence. The interdental septum reflected facial marginal bone loss in the mandibular incisors.

The I148M Variant of PNPLA3 Reduces the Response to Docosahexaenoic Acid in Children with Non-Alcoholic Fatty Liver Disease

Valerio Nobili,Giorgio Bedogni,Benedetta Donati,Anna Alisi,Luca Valenti 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.10

The aim of this secondary analysis of a randomized controlled trial was to test whether the I148M variant of Patatin-like phospholipase domain-containing protein-3 (PNPLA3) is associated with the response to docosahexaenoic acid (DHA) in children with non-alcoholic fatty liver disease (NAFLD). Sixty children with NAFLD were randomized in equal numbers to DHA 250 mg/day, DHA 500 mg/day or placebo. Coherently with the primary analysis, the probability of more severe steatosis after 24 months of DHA supplementation was 50% lower [95% confidence interval (CI) - 59% to - 42%)] in the combined DHA 250 and 500 mg/day groups versus placebo. The present secondary analysis revealed an independent effect of PNPLA3 status on the response to DHA. In fact, the probability of more severe steatosis was higher (37%, 95% CI 26– 48%) for the PNPLA3M/M versus I/M genotype and lower (- 12%, 95% CI - 21% to - 3%) for the I/I versus I/M genotype (Somers’ D for repeated measures). We conclude that the 148M allele of PNPLA3 is associated with lower response, and the 148I allele with greater response, to DHA supplementation in children with NAFLD.

New frontiers for therapeutic endoscopic ultrasound in children

Valerio Balassone,Simona Faraci,Chiara Imondi,Paola De Angelis,Tamara Caldaro,Luigi Dall’Oglio 소화기인터벤션의학회 2023 International journal of gastrointestinal interven Vol.12 No.1

Therapeutic endoscopic ultrasound is a well-established tool for several gastrointestinal disorders in adults. Diffusion in children is still limited but it can represent an effective, non-invasive option. A selection of relevant clinical cases will be presented.

Reproducibility of cone-beam computed tomographic measurements of bone plates and the interdental septum in the anterior mandible

Valerio, Claudia Scigliano,Alves, Claudia Assuncao e,Manzi, Flavio Ricardo Korean Academy of Oral and Maxillofacial Radiology 2019 Imaging Science in Dentistry Vol.49 No.1

Purpose: This study aimed to introduce a novel method to evaluate the alveolar bone and interdental septum in the anterior mandible using cone-beam computed tomography (CBCT). Materials and Methods: Fifty-six CBCT scans from adult patients were selected. The CBCT scans were obtained before and after orthodontic treatment. The following measurements were taken: width of the alveolar bone and the interdental septum, height of the interdental septum, height of the bone plates, distance between the cementoenamel junction and marginal bone crests, and vertical positioning of the mandibular incisor, using the lingual plane as a reference. To test the reproducibility and the stability of the lingual plane, a triangle was traced in the anterior mandible. The intra-class correlation coefficient(ICC) was used to determine intra- and inter-examiner agreement. The paired Student t-test was used to evaluate the area of the triangle and the reproducibility of all measurements. Results: The ICC was excellent for the alveolar bone and dental measurements (0.9989 and 0.9977, respectively), as well as for the interdental septum (0.9987 and 0.9961, respectively). The area of the triangles showed stability in the lingual plane (P>0.05). For the alveolar bone, mandibular incisor, and interdental septum measurements, no statistically significant differences were found between the 2 examiners(P>0.05), confirming the technical reliability of the measurements. Conclusion: The method used in this study provides a valid and reproducible assessment of alveolar bone dimensions in the anterior mandible measured on CBCT images.

알고리즘 관리와 노조의 역할

Valerio de Stefano,Simon Taes 한국노동연구원 2022 국제노동브리프 Vol.20 No.1

Storm of Violence, Surge of Struggle : Women in the Aftermath of Typhoon Haiyan (Yolanda)

VALERIO, Kristine Aquino 이화여자대학교 아시아여성학센터 2014 Asian Journal of Women's Studies(AJWS) Vol.20 No.1

Typhoon Haiyan, known locally as Yolanda, lashed the Philippines on November 8, 2013 affecting more than 10 percent of the country’s population. Although exploitation of the environment and unequal distribution of wealth in disaster situations are widely discussed among scholars, activists, humanitarian workers, and public intellectuals, issues pertaining to women and girls must be addressed as important as well. Thus, this paper problematizes the impact of typhoon Haiyan in the lives of women and girl survivors. It sees the effect of this calamity on the sexual and reproductive health and rights (SRHR) of women and girls and how further marginalization of women and girls during disasters increases their vulnerability to sexual and gender-based violence (SGBV). Making such an analysis, this paper responds to the call for solidarity among women, which will enable the demand for the fullest attainment of the SRHR needs of women and girls and the elimination of SGBV. Ang bagyong Haiyan, Yolanda sa lokal na pangalan, na nakaapekto ng mahigit 10% ng populasyon sa bansa ay humambalos sa Pilipinas noong Nobyembre 8, 2013. Bagaman ang pagsasamantala sa kapaligiran at ang hindi pantay na distribusyon ng kayamanan sa panahon ng kalamidad ang malawak na tinatalakay ng mga iskolar, aktibista, mangagawang humanitarian, at pampublikong intelektwal, ang mga mga isyu ng kababaihan at batang babae ay nararapat lamang na bigyan din ng kahalagahan. Kaya, ang suliranin sa papel na ito ay hinggil sa naging lagay ng bagyong Yolanda sa buhay ng mga nakaligtas na kababaihan at batang babae. Pinapaliwanag ng may akda ang epekto ng kalamidad sa karapatan sa sekswal at reproduktibong kalusugan ng mga kababaihan at batang babae at kung paanong ang higit pang marginalisasyon ng kababaihan at batang babae sa panahon ng kalamidad ay nagdaragdag sa bulnerabilidad sa sekswal at base-sa-kasarian na karahasan. Sa pamamagitan ng naturang pag-aaral, ang papel na ito ay tumutugon sa panawagan para sa pagkakaisa sa hanay ng sektor ng kababaihan, at siyang naghahawan ng daan para dinggin ang mithiin para sa ganap na pagtamo ng karapatan sa sekswal at reproduktibong kalusagan at pagtanggal ng sekswal at base-sa-kasarian na karahasan.

Aerodynamic and Aeroelastic Tool for Wind Turbine Applications

Valerio Viti,Giuliano Coppotelli,Federico de Pompeis,Pier Marzocca 한국항공우주학회 2013 International Journal of Aeronautical and Space Sc Vol.14 No.1

The present work focuses on the unsteady aerodynamics and aeroelastic properties of a small-medium sized wind-turbine blade operating under ideal conditions. A tapered/twisted blade representative of commercial blades used in an experiment setup at the National Renewable Energy Laboratory is considered. The aerodynamic loads are computed using Computational Fluid Dynamics (CFD) techniques. For this purpose, FLUENT<SUP>®</SUP>, a commercial finite-volume code that solves the Navier-Stokes and the Reynolds-Averaged Navier-Stokes (RANS) equations, is used. Turbulence effects in the 2D simulations are modeled using the Wilcox k-w model for validation of the CFD approach. For the 3D aerodynamic simulations, in a first approximation, and considering that the intent is to present a methodology and workflow philosophy more than highly accurate turbulent simulations, the unsteady laminar Navier-Stokes equations were used to determine the unsteady loads acting on the blades. Five different blade pitch angles were considered and their aerodynamic performance compared. The structural dynamics of the flexible wind-turbine blade undergoing significant elastic displacements has been described by a nonlinear flap-lag-torsion slender-beam differential model. The aerodynamic quasi-steady forcing terms needed for the aeroelastic governing equations have been predicted through a strip-theory based on a simple 2D model, and the pertinent aerodynamic coefficients and the distribution over the blade span of the induced velocity derived using CFD. The resulting unsteady hub loads are achieved by a first space integration of the aeroelastic equations by applying the Galerkin’s approach and by a time integration using a harmonic balance scheme. Comparison among two- and three- dimensional computations for the unsteady aerodynamic load, the flap, lag and torsional deflections, forces and moments are presented in the paper. Results, discussions and pertinent conclusions are outlined.

Sino-Japanese Hybrids between Writing Systems:

Valerio Luigi Alberizzi 훈민정음학회 2011 Scripta Vol.0 No.3

The most accepted explanation is the one considers the wakan konkōbun as a particular form of the written language, which, developed from Insei period (1086-1185) based on the syntax of wabun and of kanbun kundoku of the Heian period (794-1086), has in its framework typical elements of these two particular written styles and completes them with the so-called zokugo and elements of hentai kanbun. Thus, according to this point of view, the wakan konkōbun should be a form that has such peculiar characteristic features allowing us to number it with other typical forms of the Japanese written language as wabun, kanbun and hentai kanbun. The difficulty of its classification, mainly due to its hybrid nature, helped a lively discussion about it and the term was used by and large to indicate any style that combined, in different ways, classical Chinese and Japanese. As a result, from the point of view of the writing systems, distinctions between wakan konkōbun and kana majiribun are not as clear as one might like. Both can be understood as “a mixed form of Chinese and Japanese” and can be traced back to the late Heian and Kamakura periods (1185-1333). One persistent point in definitions concerns the type of kana used; hence wakan konkōbun is a mixture of Chinese characters and katakana, as in the medieval war tales such as Heike monogatari, as Japanese scholars had been unanimous in supporting that gunki monogatari may be considered as a wakan konkōbun model. A survey of the history of written Japanese, nevertheless, reveals the presence of Sino-Japanese hybrids, albeit at a latent stage, since the very beginning of Japanese history, and the evolution of wakan konkōbun doesn’t seem to be direct linked to the development of writing systems as variations in copying texts and manuscripts might alter the style of a text at surface level. The purpose of this paper is to identify some of the key features that defined the formation process of written Sino-Japanese through an analysis of some representative texts trying to focus on the controversial and scarcely debated issue of the possibility of studying wakan konkōbun from a grammatology perspective.