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Cryopreservation of Citrus madurensis Zygotic Embryos by Modified Encapsulation-dehydration
Eun Gi Cho,Yue Luan Hor,Haeng Hoon Kim,Jae Gyun Gwag,V. Ramanatha Rao,Florent Engelmann 한국육종학회 2003 한국육종학회지 Vol.35 No.3
The rol e of osm otol erance and dehydr ation, in terms of both medium com posi tion and exposur e duration, on sur vi val ofem bryos of Citrus m adurensi s after cryopr eser vation usi ng the encapsul ation-dehydr ation and modified encapsul ation-dehydr
LEE, SOK-YOUNG,FAI, WAN KAH,ZAKARIA, MYRIANA,IBRAHIM, HALIJAH,OTHMAN, ROFINA YASMIN,GWAG, JAE-GYUN,RAO, V. RAMANATHA,PARK, YONG-JIN Blackwell Publishing Ltd 2007 Molecular ecology notes Vol.7 No.6
<P>Abstract</P><P>The present study reports isolation and characterization of eight polymorphic microsatellite markers for <I>Zingiber officinale</I> Rosc. (Ginger). A total of 34 alleles were detected across the 20 accessions, with an average of 4.3 alleles per locus. Values for observed and expected heterozygosities ranged from 0 to 1.0 and from 0.23 to 0.67, respectively. The heterozygote deficits were observed at three loci. At the significance threshold (<I>P</I> < 0.05) of the eight loci, seven were found to have deviated from Hardy–Weinberg equilibrium, whereas significant linkage disequilibria were observed between 10 pairs of loci. Our data indicate the existence of moderate level of genetic diversity among the ginger accessions genotyped with eight markers.</P>