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Mazumder, Upal Kanti,Gupta, Malaya,Haldar, Pallab Kanti,Kandar, Chandi Charan Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.3
Glinus oppositifolius and Trianthema decandra belonging to the Ficoidaceae family were commonly used by tribal peoples for the treatment of liver disorders and cancer. The preliminary phytochemical screening of those plants showed the presence of flavonoids, terpenoids, tannins and saponins. The aim of this study was to evaluate the in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius (MEGO) and Trianthema decandra (METD). The antioxidative capacities of MEGO and METD were determined by the following four complementary assay; DPPH radical scavenging assay, superoxide anion generation by xanthine-xanthine Oxidase assay, hydroxyl radical scavenging assay and $Fe^{2+}$-ascorbate induced by lipid peroxidation assay. The $IC_{50}$ values of the both extracts were calculated from the inhibition curve. The $IC_{50}$ MEGO and METD in DPPH, superoxide anion, hydroxyl radical scavenging and lipid peroxidation assay are 1.85, 7.31, 13.95, 22.82 and 2.21, 9.78, 14.87, 19.76 ${\mu}g/ml$ respectively. Both the extracts exhibited a significant antioxidant effects.
Upal Kanti Mazumder,Malaya Gupta,Chandi Charan Kandar,Pallab Kanti Haldar 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.3
Glinus oppositifolius and Trianthema decandra belonging to the Ficoidaceae family were commonly used by tribal peoples for the treatment of liver disorders and cancer. The preliminary phytochemical screening of those plants showed the presence of flavonoids, terpenoids, tannins and saponins. The aim of this study was to evaluate the in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius (MEGO) and Trianthema decandra (METD). The antioxidative capacities of MEGO and METD were determined by the following four complementary assay; DPPH radical scavenging assay, superoxide anion generation by xanthine-xanthine Oxidase assay, hydroxyl radical scavenging assay and Fe2+–ascorbate induced by lipid peroxidation assay. The IC50 values of the both extracts were calculated from the inhibition curve. The IC50 MEGO and METD in DPPH, superoxide anion, hydroxyl radical scavenging and lipid peroxidation assay are 1.85, 7.31, 13.95, 22.82 and 2.21, 9.78, 14.87, 19.76 µg/ml respectively. Both the extracts exhibited a significant antioxidant effects.
In vitro anti oxidant activity of methanol extract of Clerodendrum infortunatum Linn
Sannigrahi, Santanu,Mazumder, Upal Kanti,Pal, Dilip Kumar,Parida, Sambit Kyung Hee Oriental Medicine Research Center 2009 Oriental pharmacy and experimental medicine Vol.9 No.2
The antioxidant potency of methanolic extract Clerodendrum infortunatum Linn. (MECI), which are widely used in the Indian indigenous system of medicine for different purposes, was studied. The antioxidant potential was evaluated using different established in vitro antioxidant tests viz. determination of total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, nitric oxide scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging and reductive power assay. It was found that MECI contain a high amount of polyphenolics and possesses significant free radical scavenging activity in all the assay. The higher activity was may be due to presence of richest amount of polyphenolics and flavonoids in it.
In vitro anti oxidant activity of methanol extract of Clerodendrum infortunatum Linn
Santanu Sannigrahi,Upal Kanti Mazumder,Dilip Kumar Pal,Sambit Parida 경희대학교 융합한의과학연구소 2009 Oriental Pharmacy and Experimental Medicine Vol.9 No.2
The antioxidant potency of methanolic extract Clerodendrum infortunatum Linn. (MECI), which are widely used in the Indian indigenous system of medicine for different purposes, was studied. Theantioxidant potential was evaluated using different established in vitro antioxidant tests viz. determination of total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, nitric oxide scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging and reductive power assay. It was found that MECI contain a high amount of polyphenolics and possesses significant free radical scavenging activity in all the assay. The higher activity was may be due to presence of richest amount of polyphenolics and flavonoids in it. The antioxidant potency of methanolic extract Clerodendrum infortunatum Linn. (MECI), which are widely used in the Indian indigenous system of medicine for different purposes, was studied. Theantioxidant potential was evaluated using different established in vitro antioxidant tests viz. determination of total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, nitric oxide scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging and reductive power assay. It was found that MECI contain a high amount of polyphenolics and possesses significant free radical scavenging activity in all the assay. The higher activity was may be due to presence of richest amount of polyphenolics and flavonoids in it.
Antimicrobial and lipid peroxidation inhibition activity of Oxystelma esculentum (Asclepiadaceae)
D., Ashok Kumar,V., Thamil Selvan,Saha, Prerona,Islam, Aminul,Mazumder, Upal Kanti,Gupta, Malaya Kyung Hee Oriental Medicine Research Center 2010 Oriental pharmacy and experimental medicine Vol.10 No.3
The aerial parts of methanol extract of Oxystelma esculentum (MEOE) (Asclepiadaceae) was evaluated for in vitro lipid peroxidation and antimicrobial activity. Lipid peroxidation was assayed by the change in optical density of the various concentrations (20 - 320 ${\mu}g$/ml) and the percentage inhibition was estimated. Ascorbate/FeSO4-induced peroxidation was inhibited by MEOE and standard antioxidants such as BHA, BHT and the percentage inhibition of the methanol extract was increased with dose dependent manner. The $IC_{50}$ value of the MEOE, BHA and BHT for lipid peroxidation was found to be 135.24 ${\mu}g$/ml, 25.62 ${\mu}g$/ml and 17.13 ${\mu}g$/ml, respectively. The antimicrobial activity of MEOE was determined by disc diffusion method with three grampositive, five gram-negative and two fungal microorganisms. MEOE exhibited the antimicrobial activity against the tested microorganisms except Salmonella typhimurium (MTCC 98). In present study, it is concluded that MEOE has significant effect in the inhibition of lipid peroxidation and possesses good antimicrobial activity.
Antimicrobial and lipid peroxidation inhibition activity of Oxystelma esculentum (Asclepiadaceae)
Ashok Kumar D,Thamil Selvan V,Prerona Saha,Aminul Islam,Upal Kanti Mazumder,Malaya Gupta 경희대학교 융합한의과학연구소 2010 Oriental Pharmacy and Experimental Medicine Vol.10 No.3
The aerial parts of methanol extract of Oxystelma esculentum (MEOE) (Asclepiadaceae) was evaluated for in vitro lipid peroxidation and antimicrobial activity. Lipid peroxidation was assayed by the change in optical density of the various concentrations (20 - 320 µg/ml) and the percentage inhibition was estimated. Ascorbate/FeSO4-induced peroxidation was inhibited by MEOE and standard antioxidants such as BHA, BHT and the percentage inhibition of the methanol extract was increased with dose dependent manner. The IC50 value of the MEOE, BHA and BHT for lipid peroxidation was found to be 135.24 µg/ml, 25.62 µg/ml and 17.13 µg/ml, respectively. The antimicrobial activity of MEOE was determined by disc diffusion method with three gram-positive, five gram-negative and two fungal microorganisms. MEOE exhibited the antimicrobial activity against the tested microorganisms except Salmonella typhimurium (MTCC 98). In present study, it is concluded that MEOE has significant effect in the inhibition of lipid peroxidation and possesses good antimicrobial activity.
Susanta Kumar Mondal,Goutam Chakraborty,Uttam Kumar Bhaumik,Malaya Gupta,Upal Kanti Mazumder 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.3
The defatted methanol extract of bark of Diospyros malabarica (DM) at doses of 200 and 400 mg/ kg, p.o. showed significant hypoglycemic activity on normal rats. The extract also exerted significant antihyperglycemic effect in alloxan-induced hyperglycemia and resulted in increase in plasma protein content and decrease in alkaline phosphatase, cholesterol and triglyceride levels when compared with those in the diabetic control group. However there were no significant changes in body and kidney weights of the DM extract-treated animals, compared to those of the untreated diabetic rats as a control. However, the DM extract showed a potential antioxidant activity by increasing catalase activity and reducing lipid peroxidation in liver. The results demonstrate antidiabetic activity of the defatted methanol extract of DM bark. The defatted methanol extract of bark of Diospyros malabarica (DM) at doses of 200 and 400 mg/ kg, p.o. showed significant hypoglycemic activity on normal rats. The extract also exerted significant antihyperglycemic effect in alloxan-induced hyperglycemia and resulted in increase in plasma protein content and decrease in alkaline phosphatase, cholesterol and triglyceride levels when compared with those in the diabetic control group. However there were no significant changes in body and kidney weights of the DM extract-treated animals, compared to those of the untreated diabetic rats as a control. However, the DM extract showed a potential antioxidant activity by increasing catalase activity and reducing lipid peroxidation in liver. The results demonstrate antidiabetic activity of the defatted methanol extract of DM bark.
Mondal, Susanta Kumar,Chakraborty, Goutam,Bhaumik, Uttam Kumar,Gupta, Malaya,Mazumder, Upal Kanti Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.3
The defatted methanol extract of bark of Diospyros malabarica (DM) at doses of 200 and 400 mg/kg, p.o. showed significant hypoglycemic activity on normal rats. The extract also exerted significant antihyperglycemic effect in alloxan-induced hyperglycemia and resulted in increase in plasma protein content and decrease in alkaline phosphatase, cholesterol and triglyceride levels when compared with those in the diabetic control group. However there were no significant changes in body and kidney weights of the DM extract-treated animals, compared to those of the untreated diabetic rats as a control. However, the DM extract showed a potential antioxidant activity by increasing catalase activity and reducing lipid peroxidation in liver. The results demonstrate antidiabetic activity of the defatted methanol extract of DM bark.