Effects of Elicitors on Seedling Growth, Total Polyphenol and Chlorophyll Content and Antioxidant Activity of Barley (Hordeum vulgare L.)

Timnoy Salitxay,Phonesavanh Phouthaxay,Yeoun-Gyu Pang,Yu-Chi Yeong,Prakash Babu Adhikari,Cheol-Ho Park 한국작물학회 2016 Korean journal of crop science Vol.61 No.1

This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of 8.57 μg/mg and 3.83 μg/mg, respectively while the highest carotenoid content was found in MJ ml/L treatment with 1.62 μg/mg. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE).

Effects of Elicitors on Seedling Growth, Total Polyphenol and Chlorophyll Content and Antioxidant Activity of Barley (Hordeum vulgare L.)

Timnoy Salitxay,Phonesavanh Phouthaxay,Yeoun-Gyu Pang,Yu-Chi Yeong,Prakash Babu Adhikari,박철호 한국작물학회 2016 Korean journal of crop science Vol.61 No.1

This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of 8.57 μg/mg and 3.83 μg/mg, respectively while the highest carotenoid content was found in MJ ml/L treatment with 1.62 μg/mg. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE). Keywords : Bayley (Hordeum vulgare L), Chlorophyll, DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity, Elicitation, Growth parameters, Total polyphenol

Effects of Elicitors on Seedling Growth, Total Polyphenol and Chlorophyll Content and Antioxidant Activity of Barley (Hordeum vulgare L.)

Salitxay, Timnoy,Phouthaxay, Phonesavanh,Pang, Yeoun-Gyu,Yeong, Yu-Chi,Adhikari, Prakash Babu,Park, Cheol-Ho The Korean Society of Crop Science 2016 한국작물학회지 Vol.61 No.1

This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of $8.57{\mu}g/mg$ and $3.83{\mu}g/mg$, respectively while the highest carotenoid content was found in MJ ml/L treatment with $1.62{\mu}g/mg$. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE).

Effect of Enzyme Treatment on the cell Macrophage Proliferation and Nitric Oxide(NO) Production in Common Buckwheat

Phonesavanh PHOUTHAXAY - Timnoy Salitxay - Woo, Sun Hee - Park, Cheol Ho 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.1

본문참고

Effects of Hormones and Elicitors on Plant Growth of Rice-type Tartary Buckwheat

Sun-Hee Woo, Beom Joo Choi, Timnoy Salitxay, Beom Sun Park, Kwang Jin Chang, Cheol Ho Park 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.2

This study was performed to determine the effects of various concentrations of two hormones (IAA and GA3) and four elicitors (SA, MJ, ALF and MMA) on plant growth of rice-type Tartary buck- wheat in a greenhouse. Rice-type Tartary buckwheat seeds showed a germination rate of over 98%, except for a treatment of IAA 1000 ppm showing a low germination rate of 76.7%. Plant height was the highest at GA3 1000 ppm (33.4 cm) 30 days after germination while IAA 1000 ppm was lowest (14.4 cm) that was lower than the control (21.8 cm). The number of leaves was in the order of IAA 100 ppm (12.9)>GA3 1000 ppm (10.2)>IAA 10 ppm (9.2). The fresh weight and dry weight at 80 days after germination were the highest at 9.6 g (FW) and 1.0 g (DW) per plant in IAA 100 ppm treatment, respectively. The fresh weight per plant was higher in 1 mL (3.97 g) of MJ and 1 mL (3.60 g) of ALF than in the control group (3.52 g). Dry weight per plant was also higher in MJ 1 mL (0.52 g) and ALF 1 mL (0.44 g) than the control group (0.26 g).

매실나무를 기주로 한 인공재배 겨우살이의 엽록소와 폴리페놀 함량 및 항산화 활성

이보덕,Timnoy Salitxay,Phonesavanh Phouyhaxay,성인제,박철호 한국국제농업개발학회 2016 韓國國際農業開發學會誌 Vol.28 No.3

The study examined qualities including chlorophyll, total phenol, total flavonoid content and antioxidative activity in 7-year-old mistletoe that was artificially cultivated with Japanese apricots as the host. The chlorophyll a content of the artificially cultivated Japanese apricot mistletoe leaves was 7.67 mg/g (old leaves) and 3.17 mg/g (new leaves), while the chlorophyll b content was 0.80 ~ 3.53 mg/g and 1.31 ~ 3.56 mg/g. The carotenoid content of the Japanese apricot mistletoe leaves were in the range of 0.29 ~ 2.48 mg/g, and the old leaves (1.85 mg/g) had a carotenoid content 2.1 times higher than the new leaves (0.89 mg/g). The total polyphenol content of the leaves and nodes of the 7-year-old mistletoe was 60.22 mg/100 g TAE for the leaves and 35.50 mg/100 g TAE for the nodes. Among the host trees, polyphenol content was highest (81.44 mg/100 g TAE) in the old mistletoe leaves of the Quercus mongolica, while the total flavonoid content of the Japanese apricot mistletoe was higher in the new leaves (47.89 mg/100 g RUE) than the old leaves (42.19 mg/100 g RUE). The DPPH radical scavenging ability of the Japanese apricot mistletoe scored a low 22.4 ~ 28.5% in the leaves, but was relatively high in the nodes at 52.4 ~ 80.1%. In terms of 1mg/mL concentration, the new leaves (69.1%) and old leaves (82.3%) of the Quercus monglica mistletoe displayed the highest inhibition rate. Chestnut mistletoe displayed an inhibition rate of 21.9 ~ 31.9% for the leaves, and 35.0 ~ 63.8% for the nodes. These results indicate that Japanese apricot mistletoe would be substitute oak tree mistletoe as a source of bio-active property for functional food and medicine.

Barley Malt Treated with Enzymes Increases Polyphenol Content and Antioxidant Activity

Phonesavanh Phouthaxay,유치영(Chi Young Yu),방연규,Timnoy Salitxay,김상헌,박철호 한국자원식물학회 2015 한국자원식물학회지 Vol.28 No.6

The purpose of this study was to improve the functionality of a healthy drink with examining the possibility of manufacturing different enzymes (alpha-, beta-, glucose-amylase) in barley malts (BM) produced in various malting periods. The study showed that enzyme treatment increased significantly total polyphenol content (TPC), DPPH radical scavenging activity and hydroxly radical scavenging activity in malted liquid samples (MLS) which obtained from various malting periods. The highest of TPC were found in Gluco-24M with 1.981 ㎎TAE/㎖, followed by Beta-24M and Alpha-72M with 1.878 ㎎TAE/㎖ and 1.845 ㎎TAE/㎖, respectively. The DPPH result revealed that percent of inhibition increased by 71-75% compared to the control. No statistical difference was found between MLS obtained by 24 hr of malting (24 M) and 72 hr of malting (72 M) after enzyme treatment. In addition, an increasing of hydroxyl radical was in the same trend to the TPC and DPPH. The hydroxyl radical scavenging activity of enzyme treated samples was 1,5 times higher than the control. These results suggest the possibility of enzyme application to barley malts obtained in various germination periods for improving quality and functionality of barley malts.