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        Antimicrobial efficacy of extracts and constituents fractionated from <i>Rheum tanguticum</i> Maxim. ex Balf. rhizomes against phytopathogenic fungi and bacteria

        Pham, Duong Quang,Ba, Duong Thi,Dao, Nga Thu,Choi, Gyung Ja,Vu, Thuy Thu,Kim, Jin-Cheol,Giang, Thi Phuong Ly,Vu, Hoang Dinh,Le Dang, Quang Elsevier 2017 INDUSTRIAL CROPS AND PRODUCTS Vol.108 No.-

        <P><B>Abstract</B></P> <P>The antimicrobial efficacies of extracts and constituents fractionated from <I>Rheum tanguticum</I> rhizomes were evaluated by using <I>in vitro</I> and <I>in vivo</I> bioassay against seven phytopathogenic fungi and ten pathogenic bacteria, respectively. Dichloromethane and ethyl acetate soluble extracts derived from <I>R. tanguticum</I> rhizomes effectively controlled plant diseases caused by five fungi <I>Magnaporthe oryzae</I>, <I>Blumeria graminis</I> f. sp<I>. hordei, Colletotrichum coccodes, Phytophthora infestans,</I> and <I>Puccinia recondita</I> in <I>in vivo</I> whole plant bioassay. In addition, these extracts showed strong inhibitory activity against the bacterial growth of <I>Acidovorax avenae</I> subsp. <I>cattlyae</I> (Aac), <I>Clavibacter michiganensis</I> subsp. <I>michiganensis</I>, <I>Xanthomonas arboricola</I> pv. <I>pruni</I> (Xap), and <I>Pseudomonas syringae</I> pv. <I>actinidiae</I> (Psa), with MIC values ranging from 125 to 250μg/mL. The active extracts of <I>R. tanguticum</I> rhizomes were fractionated by using various and repeated chromatographic techniques to yield seven constituents that included three anthraquinones and four stilbenes. Notably, at concentrations ranging from 75 to 300μg/mL, physcion (<B>DH03</B>) and chrysophanol (<B>DH04</B>) were significantly effective in suppressing the development of barley powdery mildew (BPM) caused by <I>B. graminis</I> f. sp. <I>hordei,</I> with control values from 80 to 96.7%. Moreover, stilbenes rhapontigenin (<B>DH02</B>) and desoxyrhapontigenin (<B>DH05</B>) had a broad spectrum and potent activity against phytopathogenic bacteria; <B>DH05</B> effectively inhibited the bacterial growth of Aac, <I>Burkholderia glumae</I> and Psa at MICs ranging from 38 to 150μg/mL. In this paper, we report their antibacterial activity for the first time. The botanical materials containing anthraquinones and stilbenes were active against both phytopathogenic fungi and bacteria. These results suggested that <I>R. tanguticum</I> rhizomes could be used as a new source of antimicrobial substances for developing a botanical fungicide and bactericide to control plant diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>R. tanguticum</I> extracts exhibited activity against phytopathogenic fungi and bacteria. </LI> <LI> Seven anthraquinones and stilbenes from <I>R. tanguticum</I> were isolated and identified. </LI> <LI> <I>In vivo</I> antifungal activity of botanicals was evaluated by the whole plant bioassay. </LI> <LI> The isolated stilbenes effectively inhibited the growth of phytopathogenic bacteria. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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        Facile synthesis of multifunctional Ag/Fe3O4-CS nanocomposites for antibacterial and hyperthermic applications

        Ngoan Thi Nguyen,Dai Lam Tran,Duc Cuong Nguyen,Thai Loc Nguyen,Thi Cham Ba,Binh Hai Nguyen,Thi Duong Ba,Nam Hong Pham,Dzung Tuan Nguyen,Thai Hoa Tran,Gia Dien Pham 한국물리학회 2015 Current Applied Physics Vol.15 No.11

        Nanocomposites containing two or more functional constituents are attractive candidates for advanced nanomaterials. In this study, multifunctional Ag/Fe3O4-CS nanocomposites were successfully prepared, using chitosan as a stabilizing and cross-linking agent. The as-synthesized nanocomposites were characterized by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Energy-dispersive X-ray spectroscopy (EDS), UVevisible spectrophotometer (UVeVis) and vibrating sample magnetometer (VSM). The results demonstrated that Ag/Fe3O4-CS composite nanoparticles (NPs) were composed of parent components, Fe3O4 and Ag NPs, which were uniformly dispersed in the chitosan matrix. The hybrid NPs exhibited strong antibacterial property against Pseudomonas aeruginosa. With high magnetization value (67 emu/g), the synthesized Ag/Fe3O4-CS composite can be easily separated or recycled in potential biomedical applications. Furthermore, the results showed that the multicomponent hybrid nanostructures appeared to be the promising material for local hyperthermia, which can be used as thermoseeds for localized hyperthermia treatment of cancers.

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