Induction of Apoptosis in Anoikis-Resistant Breast Cancer Stem Cells by Supercritical CO2 Extracts from Citrus hassaku Hort ex Tanaka

Linh Thi Thao Nguyen,송연우,Thao Anh Tran,김기석,김소미 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4

Anoikis (or cell-detachment-induced apoptosis) is aspecial form of apoptosis in which cells loose contact with othercells or the extracellular matrix. In the present study, we havefound that the breast cancer stem cell line MCF-7-SC, isolatedfrom MCF-7 breast cancer cells, shows characteristics of anoikisresistance. Treatment of supercritical CO2 extract from Citrushassaku Hort ex Tanaka, locally known as Phalsak, reduced theexpression of ATP-binding cassette sub-family G member 2mRNA and induced apoptosis in MCF-7-SCs, as evidenced by anincrease of apoptotic body formation, increased cell population inthe sub-G1 phase, increase in the Bax/Bcl-2 ratio, proteolyticactivation of caspase-9 and caspase-3, and the degradation ofpoly(ADP-ribose) polymerase (PARP) protein. The effects ofsupercritical CO2 extract from Phalsak on anoikis-resistant breastcancer stem cells suggest the use of this extract as a potentialtherapeutic agent for breast cancer stem cell treatment.

Induction of Apoptosis in Anoikis-Resistant Breast Cancer Stem Cells by Supercritical $CO_2$ Extracts from Citrus hassaku Hort ex Tanaka

Nguyen, Linh Thi Thao,Song, Yeon Woo,Tran, Thao Anh,Kim, Ki-Seok,Cho, Somi Kim The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4

Anoikis (or cell-detachment-induced apoptosis) is a special form of apoptosis in which cells loose contact with other cells or the extracellular matrix. In the present study, we have found that the breast cancer stem cell line MCF-7-SC, isolated from MCF-7 breast cancer cells, shows characteristics of anoikis resistance. Treatment of supercritical $CO_2$ extract from Citrus hassaku Hort ex Tanaka, locally known as Phalsak, reduced the expression of ATP-binding cassette sub-family G member 2 mRNA and induced apoptosis in MCF-7-SCs, as evidenced by an increase of apoptotic body formation, increased cell population in the sub-G1 phase, increase in the Bax/Bcl-2 ratio, proteolytic activation of caspase-9 and caspase-3, and the degradation of poly(ADP-ribose) polymerase (PARP) protein. The effects of supercritical $CO_2$ extract from Phalsak on anoikis-resistant breast cancer stem cells suggest the use of this extract as a potential therapeutic agent for breast cancer stem cell treatment.

Modified sprouted rice for modulation of curcumin crystallinity and dissolution enhancement by solid dispersion

Thao T. D. Tran,Thinh D. Luu,Beom‑Jin Lee,Phuong H. L. Tran 한국약제학회 2019 Journal of Pharmaceutical Investigation Vol.49 No.1

Sprouted grains, which is a natural polysaccharide, is the subject of increasing scientific interest due to many benefits for human health. The aim of the present work was to develop sprouted rice (SR) as a safe and useful material for application in dissolution enhancement of anticancer poorly water-soluble drugs such as curcumin by solid dispersions (SDs). SDs were prepared with pure SR and modified sprouted rice (MSR) by the melting method. The dissolution rate, drug crystallinity changes, molecular interactions and wettability were characterized and compared between the formulations. The use of MSR could result in a promising system for improving the dissolution rate of poorly water-soluble drugs. MSR could induce a greater amorphous state and improved wettability of drugs for dissolution enhancement compared to SR. Although both SR and MSR showed hydrogen bonding interaction, insignificant differences between SR and MSR were observed. We found that the crystallinity, interactions and wettability of the drug were significantly affected and modulated by MSR.

Application of Nanosized Zero-valent Iron-Activated Persulfate for Treating Groundwater Contaminated with Phenol

Thao, Trinh Thi,Kim, Cheolyong,Hwang, Inseong Korean Society of Soil and Groundwater Environment 2017 지하수토양환경 Vol.22 No.1

Persulfate (PS) activated with nanosized zero-valent iron (NZVI) was tested as a reagent to remove phenol from groundwater. Batch degradation experiments indicated that NZVI/PS molar ratios between 1 : 2 and 1 : 5 were appropriate for complete removal of phenol, and that the time required for complete removal varied with different PS and NZVI dosages. Chloride ions up to 100 mM enhanced the phenol oxidation rate, and nitrate of any concentration up to 100 mM did not significantly affect the oxidation rate. NZVI showed greater performance than ferrous iron did as an activator for PS. A by-product was formed along with phenol degradation but subsequently was completely degraded, which showed the potential to attain mineralization with the NZVI/PS system. Tests with radical quenchers indicated that sulfate radicals were a predominant radical. The results of this study suggest that NZVI is a promising activator of PS for treating contaminated groundwater.

Poly(γ-glutamic acid)/Chitosan Hydrogel Nanoparticles for Effective Delivery of Fermented Herbal Extract to Enhance Hair Growth

Thao Nguyen LE,Hye Su KIM,Hye-Jin PARK,Moon Il KIM 한국생물공학회 2019 한국생물공학회 학술대회 Vol.2019 No.10

My Life in Korea

Thao Nguyen 대한토목학회 2016 대한토목학회지 Vol.64 No.1

Understanding of Photocatalytic Degradation of Dissolved Organic Matter by ZnO under Artificial Sunlight using PARAFAC modelling

Thao Thi NGUYEN,Seong-Nam NAM,Jeill OH 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

Effects of Supplementation of Eucalyptus (E. Camaldulensis) Leaf Meal on Feed Intake and Rumen Fermentation Efficiency in Swamp Buffaloes

Thao, N.T.,Wanapat, M.,Kang, S.,Cherdthong, A. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.7

Four rumen fistulated swamp buffaloes were randomly assigned according to a $4{\times}4$ Latin square design to investigate the effects of Eucalyptus (E. Camaldulensis) leaf meal (ELM) supplementation as a rumen enhancer on feed intake and rumen fermentation characteristics. The dietary treatments were as follows: T1 = 0 g ELM/hd/d; T2 = 40 g ELM/hd/d; T3 = 80 g ELM/hd/d; T4 = 120 g ELM/hd/d, respectively. Experimental animals were kept in individual pens and concentrate was offered at 0.3% BW while rice straw was fed ad libitum. The results revealed that voluntary feed intake and digestion coefficients of nutrients were similar among treatments. Ruminal pH, temperature and blood urea nitrogen concentrations were not affected by ELM supplementation; however, ELM supplementation resulted in lower concentration of ruminal ammonia nitrogen. Total volatile fatty acids, propionate concentration increased with the increasing level of EML (p<0.05) while the proportion of acetate was decreased (p<0.05). Methane production was linearly decreased (p<0.05) with the increasing level of ELM supplementation. Protozoa count and proteolytic bacteria population were reduced (p<0.05) while fungal zoospores and total viable bacteria, amylolytic, cellulolytic bacteria were unchanged. In addition, nitrogen utilization and microbial protein synthesis tended to increase by the dietary treatments. Based on the present findings, it is suggested that ELM could modify the rumen fermentation and is potentially used as a rumen enhancer in methane mitigation and rumen fermentation efficiency.

Yb³⁺,Er³⁺,Eu³⁺-codoped YVO₄ material for bioimaging with dual mode excitation

Thao, Chu Thi Bich,Huy, Bui The,Sharipov, Mirkomil,Kim, Jin-Ik.,Dao, Van-Duong,Moon, Ja-Young,Lee, Yong-Ill Elsevier S.A. 2017 Materials Science and Engineering C Vol. No.

<P>We propose an efficient bioimaging strategy using Yb3+,Er3+,Eu3+-triplet doped YVO4 nanoparticles which were synthesized with polymer as a template. The obtained particles possess nanoscale, uniform, and flexible excitation. The effect of Eu3+ ions on the luminescence properties of YVO4:Yb3+,Er3+,Eu3+ was investigated. The upconversion mechanism of the prepared material was also discussed. The structure and optical properties of the prepared material were characterized by using X-ray diffraction (XRD), Fourier-transform IR spectroscopy (MR), scanning electron microscopy (SEM), Transmission electron microscopy (TEM) upconversion and photoluminescence spectra. The Commission International de I'Eclairage (CIE) chromaticity coordinates was investigated to confirm the performance of color luminescent emission. The prepared YVO4:Yb3+,Er3+,Eu3+ nanoparticles could be easily dispersed in water by surface modification with cysteine (Cys) and glutathione (GSH). The aqueous dispersion of the modified YVO4:Yb3+,Er3+,Eu3+ exhibits bright upconversion and downconversion luminescence and has been applied for bioimaging of HeLa cells. Our developed material with dual excitation offers a promising advance in bioimaging. (C) 2017 Elsevier B.V. All rights reserved.</P>

CLIMBING THE RUNGS OF LUXURY BRAND’S LADDER AND SOCIAL STATUS

Thao Nguyen,George Balabanis 글로벌지식마케팅경영학회 2014 Global Marketing Conference Vol.2014 No.7

Luxury industry is one of the fastest growing fields in marketing research in which many studies have examined how consumers' background affects their preference for luxury products and luxury brands. Classical theories focused on the affluent social classes and their tendency to consume luxury products to consciously or unconsciously signal their wealth. Other studies argued that the internalised culture developed during the socialisation years may influence one’s tastes and preferences for luxuries. Whereas, “costly signal theory” and “affirmation resource theory” suggest that luxury consumption extends beyond the traditional symbolic value of luxury brands and the habituated taste consumption drivers. Despite the wealth of current theoretical approaches, there is little research on the context of status rankings of luxuries and luxury brands. Thus, main objective of the present study is to develop and empirically test a conceptual framework that links consumption of at different rungs of the luxury brand ladder to a number of variables that may act as moderators which will be defined below. The key predictors are economic resources, cultural capital, perceived social status and desired status. The dependent variable is consumption of luxury brands at different rungs in the luxury ladder. A number of hypotheses are proposed and empirically tested. The dependent variable was measured by survey data collected from a sample of US consumers. The results confirm that the frequency of luxury brand purchases is significantly and positively related to consumers’ economic resources and desire for status. The hierarchy of luxury brands consumed is driven by consumers’ economic resources, cultural capital and desire for social status. Regardless of economic resources and perceived social status, consumers with high desire for social status are more likely to buy brands which represent high level of luxury. Likewise, people with higher cultural capital would buy higher luxury grading brands regardless of their economic capabilities. Moreover, results showed that perceived social status is negatively correlated with preferences for higher tier luxury brands of cars. In particular, desire for social status seems to moderates the effect of perceived social status on the hierarchy of brand of luxury car preferences. Desire for status combined with high perceived social status leads to higher preference for higher tier luxury products. It can be concluded that economic resources are responsible for the frequency people buy luxury brands, whereas cultural capital is responsible for the luxury hierarchy of the consumed brands. Frequent consumption of luxury brands reflects one’s economic status whereas the grade of luxury brands his/her cultural status. However, desire for status seems to be a key variable as it affects both frequency and the ranks of luxury brands purchased. This is an important variable as desire for status is not related with neither the actual social status (i.e., economic resources and cultural capital) nor perceived social status.