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Hui Teng,Kashif Ghafoor,Yong Hee Choi 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.6
Active components in Chinese quince were extracted by using high efficient microwave-assisted extraction. In accordance with central composited design, 20 runs with 3 variables and 5 levels applied for the optimization in response surface methodology syste
Teng, Hui,Choi, Yong-Hee The Korean Society for Applied Biological Chemisty 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.2
Alkaloids, a group of second metabolites from plants, possess great health benefits against various chronic diseases. Rhizome coptidis (Coptis chinensis Franch) is a commonly used traditional Chinese herb that contains abundant alkaloids but rarely used. In the present study, the effects of process variables on alkaloid content from rhizome coptidis were investigated, and extraction temperature above $80^{\circ}C$ was found to have insignificant effect. To achieve maximal extraction of alkaloids from rhizome coptidis, a five-level, three-variable small composite design was employed, and optimal conditions of extraction time, ethanol concentration, and solvent to sample ratio were obtained using response surface methodology. Based on the analysis of variance results, extraction time, and solvent to sample ratio were identified as critical variables in extraction process, and the optimum extraction conditions were an extraction time of 133 min, ethanol concentration of 45%, and solvent to sample ratio of 42 v/w, and under these conditions, the experimental total alkaloid content was $15.96{\pm}0.15$ g BCE/100 g, which well matched with the predicted value.
Teng, Hui,Jo, In-Hee,Choi, Yong-Hee The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.5
Functional phenolic compounds were extracted from Chinese quince using highly efficient ultrasonic-assisted extraction (UAE) technology. The experiment was carried out in accordance with a small central composite design (SCCD) five-level and three-variable approach. The optimum combination of solvent concentration, extraction temperature and time with the application of ultrasonic, for the maximum extraction of phenolic compounds from Chinese quince, was obtained by response surface methodology. Responses of phenolic compounds such as total phenolics, total flavonoids, and total flavan-3-ols were determined by the spectrophotometer assay. High significance of each response revealed that this SCCD model well-fitted the experimental parameters as well as the reliability of the predicted value. The optimal UAE conditions obtained by superimposed contour plot were 50% ethanol concentration, $63.9^{\circ}C$ extraction temperature, and extraction time 11.4 min. Under the above-mentioned conditions, the experimental results for three responses were total phenolic content 2367.16 mg gallic acid equivalents /100 g, total flavonoid content 544.12 mg rutin equivalents /100 g, and total flavan-3-ols content 709.07 mg catechin equivalents /100 g, which well-matched with the predicted values. The optimal conditions attained from the present study would provide scientific references to economical production of relative phenolic compounds products.
Teng, Hui,Ghafoor, Kashif,Choi, Yong-Hee The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.6
Active components in Chinese quince were extracted by using high efficient microwave-assisted extraction. In accordance with central composited design, 20 runs with 3 variables and 5 levels applied for the optimization in response surface methodology system. The extracts were analyzed by spectrophotometeric methods for the total flavonoids content (TFC), total phenolic compound (TPC) and electron donating ability (EDA). The optimum conditions obtained were 44.15% ethanol concentration, 5 min extraction time and 102.1 W microwave power for maximum TFC (477.8 mg RE/100 g), TPC (2249 mg GAE/100 g) and EDA (77.47%) as predicted by overlaid contour plots.
Teng, Hui,Lee, WonYoung Institute of Agricultural Science and Technology 2014 慶北大農學誌 Vol. No.
Mulberry fruits were semi-dried using hot air ($60-100^{\circ}C$) or cool air ($20-40^{\circ}C$), and the effects of the drying temperature and processing time on the quality of the final dried mulberry fruits were investigated. Response surface methodology was employed to establish a statistical model and predict the conditions resulting in minimal loss of the total phenolic content (TPC) and ascorbic acid. Thus, using overlapped contour plots, the optimal conditions for producing semi-dried mulberry fruits, which reduced the moisture residue to 45% and minimized the nutrient losses of TPC and ascorbic acid, were determined for the hot-air process ($60.7^{\circ}C$ for 5.4 h) and cool-air process ($34.8^{\circ}C$ for 23.3 h). Plus, a higher drying temperature was found to lead to a faster loss of moisture and ascorbic acid, while the TPC was significantly decreased in the cool-air dried mulberry fruits due to the higher activity of polyphenol oxidase between 30 and $40^{\circ}C$.
Teng, Hui,Lee, WonYoung 경북대학교 농업생명과학대학 2014 Current Research on Agriculture and Life Sciences Vol.32 No.2
Mulberry fruits were semi-dried using hot air (60-100℃) or cool air (20-40℃), and the effects of the dryingtemperature and processing time on the quality of the final driedmulberry fruits were investigated. Response surface methodologywas employed to establish a statistical model and predict theconditions resulting in minimal loss of the total phenolic content(TPC) and ascorbic acid. Thus, using overlapped contour plots,the optimal conditions for producing semi-dried mulberry fruits,which reduced the moisture residue to 45% and minimized thenutrient losses of TPC and ascorbic acid, were determined for thehot-air process (60.7℃ for 5.4h) and cool-air process (34.8℃ for23.3h). Plus, a higher drying temperature was found to lead to afaster loss of moisture and ascorbic acid, while the TPC wassignificantly decreased in the cool-air dried mulberry fruits due tothe higher activity of polyphenol oxidase between 30 and 40℃.
Hui Teng,최용희 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.3
The optimum conditions for the extraction of bioactive alkaloids and antioxidants from rhizome coptidis were determined by using response surface methodology (RSM). A small central composite design (SCD) was employed to investigate the effect of 3 process variables such as extraction time (3-7 h), ethanol concentration (0-100%), and solvent to sample ratio (25-45 mL/g) during extraction process. Analysis of variance showed a high significance of fitted model which indicated the model is reliable for prediction. The optimal conditions for alkaloids and antioxidant ability were obtained using ridge analysis,and under the optimum conditions the corresponding predicted response values for berberine content, palmatine content, and antioxidant ability were 747.2 μg berberine chloride equivalent/mL, 168.34 μg palmatine chloride equivalent/mL, and 2.99 mmol/L Trolox equivalent,respectively, which well agreed with predicted values,indicating the success of RSM in optimizing the extraction conditions.
Hui Teng,최용희 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.5
Microwave-assisted extraction (MAE) technique was employed and optimized for microwave power,irradiation time and solvent (ethanol) concentration using a central composite design and response surface methodology for the efficient extraction of bioactive alkaloid compounds from Rhizoma coptidis (Coptis chinensis Franch.). Alkaloid compounds were successfully isolated and quantified by high-performance liquid chromatography with ultraviolet detection. Maximum yields of alkaloid constitutes were predicted based on desirability option, and optimal conditions for MAE were microwave power of 180W, irradiation time of 5min, and ethanol concentration of 50%. Reliability of the method was confirmed by verification experiments performed under optimal conditions, and the experimental values (TAC of 333.94 mg BCE/g d.w., BC of 71.43 mg BCE/g d.w., and PC of 15.58 mg PCE/g d.w.) matched well with estimated values, suggesting that the estimated models were reliable and valid for MAE of alkaloids.
Hui-Juan Teng,Yue Guo,Jian-Qi Wang,Rongjun Li,Ying-Tang Lu,Lei Zhang 한국식물학회 2016 Journal of Plant Biology Vol.59 No.6
CRK5 is a member of the Arabidopsis thaliana Ca2+-dependent protein kinase-related kinase family. Here, a yeast two-hybrid screen was performed with a truncated form of AtCRK5 as bait to identify interacting proteins and determine its physiological roles. One gene encoding the DWD protein WDRP was isolated. Furthermore, in vitro and in vivo co-immunoprecipitation results strongly supported that these two proteins interact with each other. Using a cell-free degradation assay, we also established that CRK5 was an unstable protein that was degraded through the proteasome pathway. The rate of CRK5 degradation was delayed in a WDRP knockout line. On the other hand, the degradation of CRK5 mediated by WDRP might not affect the phosphorylation of PIN2 by CRK5. Overall, we demonstrated that AtCRK5 interacted with a DWD protein, AtWDRP; the protein AtWDRP targets the kinase for ubiquitin-dependent degradation. Therefore, this report describes a new kinase regulation pathway for CRK family proteins in Arabidopsis.