Cotton GhKCH2, a Plant-specific Kinesin, is Low-affinitive and Nucleotide-independent as Binding to Microtubule

( Tao Xu ),( Xue Wei Sun ),( Shi Ling Jiang ),( Dong Tao Ren ),( Guo Qin Liu ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5

Nickle nanoparticles highly dispersed on reduced graphene oxide for ammonia decomposition to hydrogen

Tao Meng,Tie-Zhen Ren,Qian-Qian Xu,Yin-Tao Li,Jian-Li Chang,Zhong-Yong Yuan 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.32 No.-

The Ni/reduced graphene oxide catalysts are synthesized by an in situ hydrothermal method, using thegraphene oxide as the support precursor. The textural and structural properties of the prepared Ni/reduced graphene oxide catalysts are characterized by X-ray diffraction, scanning and transmissionelectron microscopy, thermogravimetric analysis, H2-Temperature-programmed reduction, andelectrochemical impedance spectroscopy. The catalytic performances of the prepared Ni/reducedgraphene oxide catalysts for ammonia decomposition display the enhanced activity. The effect ofreduced graphene oxide support and the influence of nickel content on catalytic activity are evaluated,and the Ni/reduced graphene oxide catalysts exhibit higher catalytic activity than reduced grapheneoxide support and the pure NiO. The ammonia has a conversion of 81.9% and 27.4 mmol/min gcat H2 rateat 700 8C when the 10%-Ni/reduced graphene oxide catalyst used.

Low Power Digital Logic Gate Circuits Based on N-Channel Oxide TFTs

임도(Tao Ren),박기찬(KeeChan Park),오환술(HwanSool Oh) 大韓電子工學會 2011 電子工學會論文誌-SD (Semiconductor and devices) Vol.48 No.3

N-channel 산화물 박막 트랜지스터(Thin Film Transistor, 이하 TFT)만을 이용한 저소비전력 inverter, NAND, NOR의 논리 게이트 회로를 제안한다. 제안된 회로는 asymmetric feed-through와 bootstrapping을 이용해서 pull-up, pull-down 스위치가 동시에 켜지지 않도록 설계하였다. 그 결과로 출력신호 전압 범위가 입력신호 전압과 동일하고 정전류가 흐르지 않는다. 인버터는 5개의 TFT와 2개의 capacitor로, NAND 및 NOR 게이트는 각각 10개의 TFT와 4개의 capacitor로 구성된다. 산화물 TFT 모델을 사용하여 SPICE 시뮬레이션을 수행하여 제안된 회로의 동작을 성공적으로 검증하였다. Low-power logic gates, i.e. inverter, NAND, and NOR, are proposed employing only n-channel oxide thin film transistors (TFTs). The proposed circuits were designed to prevent the pull-up and pull-down switches from being turned on simultaneously by using asymmetric feed-through and bootstrapping, thereby exhibited same output voltage swing as the input signal and no static current. The inverter is composed of 5 TFTs and 2 capacitors. The NAND and the NOR gates consist of 10 TFTs and 4 capacitors respectively. The operations of the logic gates were confirmed successfully by SPICE simulation using oxide TFT model.

Bavachin Suppresses Alpha-Hemolysin Expression and Protects Mice from Pneumonia Infection by Staphylococcus aureus

( Ye Tao ),( Dazhong Sun ),( Xinran Ren ),( Yicheng Zhao ),( Hengjian Zhang ),( Tao Jiang ),( Jiyu Guan ),( Yong Tang ),( Wu Song ),( Shuqiang Li ),( Li Wang ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

Staphylococcus aureus (S. aureus) infection causes dramatic harm to human health as well as to livestock development. As an important virulence factor, alpha-hemolysin (hla) is critical in the process of S. aureus infection. In this report, we found that bavachin, a natural flavonoid, not only efficiently inhibited the hemolytic activity of hla, but was also capable of inhibiting it on transcriptional and translational levels. Moreover, further data revealed that bavachin had no neutralizing activity on hla, which did not affect the formation of hla heptamers and exhibited no effects on the hla thermal stability. In vitro assays showed that bavachin was able to reduce the S. aureus-induced damage of A549 cells. Thus, bavachin repressed the lethality of pneumonia infection, lung bacterial load and lung tissue inflammation in mice, providing potent protection to mice models in vivo. Our results indicated that bavachin has the potential for development as a candidate hla inhibitor against S. aureus.

An Integrated Artificial Neural Network-based Precipitation Revision Model

( Tao Li ),( Wenduo Xu ),( Li Na Wang ),( Ningpeng Li ),( Yongjun Ren ),( Jinyue Xia ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.5

Precipitation prediction during flood season has been a key task of climate prediction for a long time. This type of prediction is linked with the national economy and people's livelihood, and is also one of the difficult problems in climatology. At present, there are some precipitation forecast models for the flood season, but there are also some deviations from these models, which makes it difficult to forecast accurately. In this paper, based on the measured precipitation data from the flood season from 1993 to 2019 and the precipitation return data of CWRF, ANN cycle modeling and a weighted integration method is used to correct the CWRF used in today’s operational systems. The MAE and TCC of the precipitation forecast in the flood season are used to check the prediction performance of the proposed algorithm model. The results demonstrate a good correction effect for the proposed algorithm. In particular, the MAE error of the new algorithm is reduced by about 50%, while the time correlation TCC is improved by about 40%. Therefore, both the generalization of the correction results and the prediction performance are improved.

Effects of Selenizing Codonopsis pilosula Polysaccharide on Macrophage Modulatory Activities

( Tao Qin ),( Zhe Ren ),( Dandan Lin ),( Yulong Song ),( Jian Li ),( Yufang Ma ),( Xuehan Hou ),( Yifan Huang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.8

The purpose of the present study was to investigate the immune-enhancing activity of selenizing Codonopsis pilosula polysaccharide (sCPPS5) in nonspecific immune response. In in vitro experiment, the results showed that sCPPS5 could promote the phagocytic uptake, NO production, and TNF-α and IL-6 secretion of RAW264.7 cells. sCPPS5 could also strongly increase the IκB-α degradation in the cytosol and the translocation of NF-κB p65 subunit into the nucleus of RAW264.7 cells. In the vivo experiment, sCPPS5 at medium doses could significantly improve the phagocytic index of peritoneal macrophages and induce the secretion of TNF-α and IL-6. Moreover, the effect of sCPPS5 was significantly better than Codonopsis pilosula polysaccharide (CPPS). These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of CPPS in the nonspecific immune response.

Antiproliferative activities of Garcinia bracteata extract and its active ingredient, isobractatin, against human tumor cell lines

Tao Shen,Wei Li,Yan-Yan Wang,Qing-Qing Zhong,Shu-Qi Wang,Xiao-Ning Wang,Dong-Mei Ren,Hongxiang Lou 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

In our cell based screening of antitumoringredients from plants, the EtOH extract of Garciniabracteata displayed antiproliferative effect against humanlung adenocarcinoma A549 cells, human breast cancerMCF-7 cells, and human prostate cancer PC3 cells. Phytochemicalinvestigation of this active extract producednine ingredients, and their structures were established byanalysis of MS and NMR spectra. Antiproliferative evaluationof isolated ingredients on A549, MCF-7 and PC3cells indicated that a xanthone named isobractatin (1)exhibited potent antiproliferative activity against the abovethree human cancer cell lines with IC50 values rangingfrom 2.90 to 4.15 lM. Treatment of PC3 cells with 1 led toan enhancement of the cell apoptosis, and arrested cellcycle in the G0/G1 phase. The G0/G1 phase cycle-relatedproteins analysis showed that the expressions of cyclins D1and E were reduced by 1, whereas the protein level of cyclin dependent kinase (CDK) inhibitor P21 was induced. Additionally, 1 enhanced PC3 cell apoptosis by activationsof Bax, caspases 3 and 9, and by inhibition of Bcl-2. Ourcombined data illustrated that isobractatin (1) was theantiproliferative ingredient of G. bracteata against threehuman cancer cell lines, which exerted its antiproliferatriveeffect via cell cycle arrest and induction of apoptosis.

Protocadherin alpha 3 inhibits lung squamous cell carcinoma metastasis and epithelial-mesenchymal transition

Tao Yu,Fei Liu,Chang Liu,Yongyu Liu,Jianhui Jia,Yanan Liu,Yi Ren 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Lung squamous cell carcinoma (LUSC) is associated with poor clinical prognosis and lacks available targeted therapy. Given that the major threat of cancer is metastasis, delineation of the molecular mechanism underlying it would help devise therapeutic strategies. Objective: To investigate the functional role of protocadherin alpha 3 (PCDHA3) in LUSC, as well as investigate the underlying molecular mechanism. Methods: Data for PCDHA3 expression and clinical information in The Cancer Genome Atlas (TCGA) were extracted and analyzed in the UALCAN platform. Expression levels of PCDHA3 in LUSC cell lines were analyzed via RT-PCR and western blot. Overexpression of PCDHA3 was conducted via plasmid transfection. CCK-8 and cell cycle assays were utilized to investigate effect of PCDHA3 on cell proliferation. Transwell assay was used to detect migration and invasion. The underlying mechanism was demonstrated via western blot analysis. Results: Our data indicate that PCDHA3 was low expressed in three kinds of LUSC cell lines and best in H520 cells. Furthermore, overexpression of PCDHA3 could significantly impair LUSC cells proliferation, invasion and migration. Moreover, PCHDA3 repressed the biomarkers of mesenchymal (N-cadherin, fibronectin and vimentin) and increased expression of epithelial markers (E-cadherin and α-catenin). On the other hand, PCDHA3 overexpression partially blocked epithelial-mesenchymal transition. Conclusions: PCDHA3 suppressed the LUSC cells proliferation, invasion and migration via inhibiting the expression of EMT signatures, suggesting that PCDHA3 could serve as a valuable therapeutic target for LUSC therapy.

Anti-HER-2×anti-CD3 Bi-specific Antibodies Inhibit Growth of HCT-116 Colorectal Carcinoma Cells in Vitro and in Vivo

Ren, Hui,Li, Jun,Liu, Jing-Jing,Guo, Hui-Ling,Jiang, Tao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Objective: This study is conducted to evaluate the effects of anti-HER-2${\times}$anti-CD3 bi-specific antibodies(BsAb) on HER-2/neuover-expressing human colorectal carcinoma cells. Methods: Growth was assessed by MTT assays after exposure of HCT-116 cells to Herceptin, anti-CD3 and BsAb antibodies. Immunocytochemistry was applied to test the HER-2 level of HCT-116. In a nude mouse model, HER-2${\times}$CD3 BsAb was combined with effector cells (peripheral blood lymph cells from normal human being) for observations on in Vivo growth of tumors. Results: Compared with the control group, using effector cells combined with anti-CD3 McAb, Herceptin or HER2${\times}$CD3 BsAb, tumor cell growth in vitro and in vivo was significantly inhibited (P<0.05), most remarkably in the HER2${\times}$CD3 BsAb case. The growth of xenografts with HER2${\times}$CD3 BsAb combined with effector cells was also significantly inhibited when compared with the anti-CD3 McAb or Herceptin groups (P<0.05). Conclusion: HER-2/neu might be a useful target for immunotherapy in colorectal carcinoma, anti-HER2${\times}$anti-CD3 BsAb exerting clear anti-tumor effects.

Glypican 2 regulates cell proliferation and metastasis in thyroid cancer cells

Tao Zhang,Yahong Liu,Xuan Ren,Zhuanping Wang,Hongjuan Wang 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Background Glypicans play a role in the growth and metastasis of several human cancers. However, little is known about its function in thyroid cancer. Therefore, this study aimed to investigate the specific functions of glypican 2 (GPC2) in thyroid cancer cells. Objective The expression of six glypican family members (GPC1 to GPC6) was detected in different thyroid cancer cell lines. The abnormal expression of GPC2 was confirmed by immunohistochemistry in or around thyroid tumor tissues. It was overexpressed and silenced in highly (TPC1 and FTC-133) and poorly (ARO) differentiated thyroid cancer cell lines, respectively. Subsequently, its role in cell proliferation, apoptosis, migration, and invasion was investigated. Results We observed that almost all glypican family members were highly expressed in thyroid cancer cells, and GPC2 expression was higher in poorly differentiated thyroid cancer cell lines than highly differentiated cell lines. The knockdown of GPC2 inhibited cell proliferation, invasion, and migration and promoted apoptosis in the ARO cells, whereas GPC2 overexpression promoted cell proliferation, invasion, and migration and inhibited apoptosis in the TPC1 and FTC-133 cells. Conclusions GPC2 is highly expressed in thyroid cancer tissues and regulates cell viability, apoptosis, migration, and invasion.