Fine mapping and candidate gene analysis of the dwarf gene d162(t) in rice (Oryza sativa L.)

Fan-tao Zhang,Xiao-ling Gao,Ping-rong Wang,Chang-hui Sun,Bing Wang,Xiu-lan Li,Jian-qing Zhu,Xiao-jian Deng 한국유전학회 2011 Genes & Genomics Vol.33 No.1

In our previous study, d162(t), a single recessive gene, which caused rice dwarf mutant, had been mapped on the short arm of chromosome 3. In this study, the d162(t) gene was fine mapped to a confined region about 0.82 cM by RM14641 and RM3134, and co-segregated with InDel361-2, InDel361-3,InDel361-5, RM14645, RM1022 and RM14643, where no known gene involved in plant height has been identified. Based on the annotation results of TIGR, dozens of open reading frames (ORFs) were predicted in this region, among them,five ORFs were the most possible genes related to the phenotype. In these ORFs, Os03g13010, related to U-box domain containing protein, had a 62bp segment deletion in the coding region in 162d (mutant type, MT). The results of RT-PCR showed that the transcriptional level of Os03g13010was significantly different between Shuhui162 (wild type, WT)and 162d (MT). Therefore, the gene (Os03g13010) encoding a U-box domain containing protein was considered as the candidate gene of d162(t).

Design Space Methodology and Its Application in Interior Permanent Magnet Motor Design

Fan, Tao,Li, Qi,Wen, Xuhui,Xu, Longya Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.3

An innovative interpretation of the per-unit interior permanent magnet (IPM) machine model known as Design Space is presented in this paper. Based on the proposed Design Space formulation, an effective computation method to predict IPM machine performance factors, such as the current and power factor in a full range of speeds, is proposed. A systematic methodology is summarized, which translates the full speed range machine design procedure into the region determination on the so-called Design Space. The effect of dc-link voltage is also analyzed in a similar manner with the current and power factor. A series of IPM motors have been designed, and a preferred motor is selected with the help of the proposed Design Space Methodology (DSM), which has the best tradeoff between the nominal voltage and the dropped voltage condition. Experiment results show that the selected motor satisfies the machine requirements and all the design constrains, such as the current and back-EMF limitations.

Doping palladium with tellurium for the highly selective electrocatalytic reduction of aqueous CO ₂ to CO

Tao, Hengcong,Sun, Xiaofu,Back, Seoin,Han, Zishan,Zhu, Qinggong,Robertson, Alex ,W.,Ma, Tao,Fan, Qun,Han, Buxing,Jung, Yousung,Sun, Zhenyu Royal Society of Chemistry 2018 Chemical Science Vol.9 No.2

<▼1><P>The doping of Pd with a small amount of Te can selectively convert CO<SUB>2</SUB> to CO with a low overpotential.</P></▼1><▼2><P>Designing highly selective and energy-efficient electrocatalysts to minimize the competitive hydrogen evolution reaction in the electrochemical reduction of aqueous CO<SUB>2</SUB> remains a challenge. In this study, we report that doping Pd with a small amount of Te could selectively convert CO<SUB>2</SUB> to CO with a low overpotential. The PdTe/few-layer graphene (FLG) catalyst with a Pd/Te molar ratio of 1 : 0.05 displayed a maximum CO faradaic efficiency of about 90% at –0.8 V (<I>vs.</I> a reversible hydrogen electrode, RHE), CO partial current density of 4.4 mA cm<SUP>–2</SUP>, and CO formation turnover frequency of 0.14 s<SUP>–1</SUP> at –1.0 V (<I>vs.</I> a RHE), which were 3.7-, 4.3-, and 10-fold higher than those of a Pd/FLG catalyst, respectively. Density functional calculations showed that Te adatoms preferentially bind at the terrace sites of Pd, thereby suppressing undesired hydrogen evolution, whereas CO<SUB>2</SUB> adsorption and activation occurred on the high index sites of Pd to produce CO.</P></▼2>

Does H3K27M Mutation Impact Survival Outcome of High-Grade Spinal Cord Astrocytoma?

Fan Zhang,Lei Cheng,Ze Ding,Shengxi Wang,Xingang Zhao,Zijun Zhao,Cong Liang,Kun Wu,Dongao Zhang,Yinqian Wang,Tao Fan 대한척추신경외과학회 2023 Neurospine Vol.20 No.4

Objective: To evaluate the impact of H3K27M mutation in the prognosis of histological high-grade intramedullary astrocytoma. Methods: A total of 78 patients who were diagnosed with high-grade spinal cord astrocytoma were included. Clinical data consisting demographic, radiological, molecular features and treatment data were recorded. Univariate and multivariate Cox analysis were performed to investigate variables associated with survival outcome of histological high-grade spinal cord astrocytoma. Results: Median survival time was 21 months. Overall survival (OS) at 1 and 3 years was 65.7% and 40.7%, respectively. Sex, location, and tumor span did not present significant association with OS. Patients with H3K27M mutation showed significant shorter duration of symptom than patients with H3K27 wild-type. As respect to adjuvant treatment, adjuvant radiotherapy and chemotherapy were associated with favorable OS (both p = 0.01). Younger patients (age ≤ 18 years) had shorter OS (p = 0.008) than adult patients (age > 18 years). Of note, H3K27M mutation did not show significant impact on the survival outcome, regardless of histology grade 3 or grade 4 (p = 0.3). Conclusion: Histological high-grade spinal cord astrocytoma has dismal prognosis. Our study demonstrated that H3K27M mutation did not show significant impact on survival outcome of histological high-grade spinal cord astrocytoma.

Experimental Evaluation on Defuzzification of TSK-type-based Interval Type-2 Fuzzy Inference Systems

Tao Zhan,Wen-Tao Li,Bing-Jiao Fan,Shuai Liu 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.4

With A2-C0 and A2-C1 models, this study conducted a series of experimental evaluation on four kinds of type reduction and defuzzification algorithms, namely Karnik-Mendel iterative procedure (KM), Nie-Tan method (NT), q factor method (QF) and modified q factor method (MQ), to show which algorithm exhibits better performance. In the experimental part, the accuracy, computational cost and stability of these methods are compared, and the accuracy between different defuzzification methods are analyzed statistically with the Wilcoxon Nonparametric Test. It is shown that for both A2-C0 and A2-C1 models, the NT, QF and MQ method could save around 80% development time than KM. The associated improvement for the accuracy and stability caused by KM are mainly concluded as: 1) for the A2-C0 model, less than 5% and around 10% than NT, about 0% and less than 5% than QF and around 20% and about 40% than MQ; 2) for the A2-C1 model, more than 60% and over 70% than NT and less than 20% and more than 30% for both QF and MQ. It becomes clear that for the A2-C0 model, the NT and QF method are better choices; while for the A2-C1, KM is a better choice when high accuracy and stability are the prerequisites but if the accuracy, stability and cost of development time are considered synchronously, QF and MQ are better. During the design of the A2-C0 and A2-C1 structures, the reported results provide us a map for the most suitable selection of the defuzzification approach among these four kinds of methods.

Preintervention risk stratification of renal pelvic cancer and ureteral cancer should differ

Tao Yang,Nan Zhang,Bo Yang,Dalin He,Junjie Fan,Jinhai Fan 대한비뇨의학회 2020 Investigative and Clinical Urology Vol.61 No.4

Purpose: To identify different preintervention prognostic factors between renal pelvic cancer (RPc) and ureteral cancer (Uc) and to develop different preintervention risk stratifications for each cancer type. Materials and Methods: A total of 1,768 patients with organ-confined upper urinary tract urothelial carcinoma (1,067 patients with RPc and 701 with Uc) who presented between 2004 and 2015 were selected from the Surveillance, Epidemiology, and End Results database. Clinicopathologic characteristics were compared between RPc and Uc. Univariable and multivariable Cox regression models were used to examine the prognostic ability of the clinicopathologic characteristics with respect to oncology outcomes. Results: Age greater than 75 years was significantly associated with cancer-specific survival (CSS) in RPc patients but not in Uc patients. Tumor size had a significant influence on CSS in Uc patients but not in RPc patients; in contrast, age had an influence in RPc but not in Uc. Unlike CSS, age was significantly associated with overall survival (OS) in both RPc and Uc. Tumor size had an effect on OS in Uc patients but not in RPc patients. Conclusions: The preintervention prognostic factors differed between RPc and Uc. Thus, we should develop separate preintervention risk stratification standards for RPc and Uc. Using these specific preintervention risk stratifications, we may be able to select the most appropriate surgical options for patients in the clinic.

Shear resistance of steel–concrete–steel deep beams with bidirectional webs

Yu-Tao Guo,Xin Nie,Jiansheng Fan,Mu-Xuan Tao 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.3

Steel–concrete–steel composite structures with bidirectional webs (SCSBWs) are used in large-scale projects and exhibit good mechanical performances and constructional efficiency. The shear behaviors of SCSBW deep beam members in key joints or in locations subjected to concentrated forces are of concern in design. To address this issue, experimental program is investigated to examine the deep-beam shear behaviors of SCSBWs, in which the cracking process and force transfer mechanism are revealed. Compared with the previously proposed truss model, it is found that a strut-and-tie model is more suitable for describing the shear mechanism of SCSBW deep beams with a short span and sparse transverse webs. According to the experimental analyses, a new model is proposed to predict the shear capacities of SCSBW deep beams. This model uses strut-and-tie concept and introduces web shear and dowel action to consider the coupled multi mechanisms. A stress decomposition method is used to distinguish the contributions of different shear-transferring paths. Based on case studies, a simplified model is further developed, and the explicit solution is derived for design efficiency. The proposed models are verified using experimental data, which are proven to have good accuracy and efficiency and to be suitable for practical application.

Selective removal of iron from aqueous solution using ion imprinted thiocyanato-functionalized silica gel sorbents

Hong-Tao Fan,Ting Sun 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.6

We combined surface imprinting technique with sol-gel process for preparing a new iron(III)-imprinted thiocyanato-functionalized silica gel sorbent using 3-thiocyanatopropyltriethoxysilane as the functional monomer, and epichlorohydrin as the cross-linking agent for the selective removal of iron(III) from aqueous solution. The iron(III)-imprinted thiocyanato-functionalized silica gel was characterized by FT-IR spectra, SEM, and the static adsorptiondesorption method. Maximum binding capacity, optimum pH, and equilibrium binding time were found to be 20.31mg·g−1, pH 4.2, and 20min, respectively. The relative selectivity coefficients of Fe(III)-imprinted thiocyanato-functionalized silica gel sorbents for Fe(III)/Ni(II), Fe(III)/Co(II), Fe(III)/Cd(II) and Fe(III)/Pb(II) were 11.8, 10.6, 8.2 and 9.1times greater than the non-imprinted silica gel, respectively. The iron(III)-imprinted thiocyanato-functionalized silica gel sorbents could be used repeatedly without decreasing in their adsorption capacities significantly.

Structure and Performances of xLiFePO4/C·(1 − x)Li3V2(PO4)3/C Cathode for Lithium-Ion Batteries by Using Poly(vinyl alcohol) as Carbon Source

Chang-ling Fan,Wei-hua Zhang,Tao-tao Zeng,Ling-fang Li,Xiang Zhang,Shao-chang Han 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.11

Poly(vinyl alcohol), whose pyrolysis carbon possesses high conductivity of 8.88 × 10−1 S/cm, was used to synthesize xLiFePO4/C·(1 − x)Li3V2(PO4)3/C cathode. It was characterized by X-ray diffraction, scanning electron microscopy, conductivity, cyclic voltammetry, and galvanostatic charge and discharge experiments. Results show that LiFePO4/C and Li3V2(PO4)3/C coexists in the cathode. The particles sizes of 0.75LiFePO4/C·0.25Li3V2(PO4)3/C (x = 0.75) are much smaller than 100 nm due to the role of poly(vinyl alcohol). Its conductivity is 8.79 × 10−2 S/cm. The oxidative and reductive peaks in cyclic voltammetry are sharp and symmetrical. Their low potential gaps indicate that the extractions and insertions of lithium ion possess excellent reversibility. Its discharge capacities at 1 and 5 C are 141.1 and 100.1 mAh/g. The more Li3V2(PO4)3/C in cathode results in the deterioration of electrochemical performances due to its low theoretical capacity. It is concluded that poly(vinyl alcohol) is an effective carbon source in the preparation of xLiFePO4/C·(1 − x)Li3V2(PO4)3/C composite cathode with excellent performances.

Icariside II Promotes the Differentiation of Adipose Tissue-Derived Stem Cells to Schwann Cells to Preserve Erectile Function after Cavernous Nerve Injury

Tao Zheng,Tian-biao Zhang,Chao-liang Wang,Wei-xing Zhang,Dong-hui Jia,Fan Yang,Yang-yang Sun,Xiao-ju Ding,Rui Wang 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.6

Icariside II (ICA II) is used in erectile dysfunction treatment. Adipose tissue-derived stem cells (ADSCs) are efficient at improving erectile function. This study aimed to explore the action mechanism of ADSCs in improving erectile function. ADSCs were isolated from the adipose tissues of rats. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay. The expressions of mRNA and protein were determined separately through qRT-PCR and western blot. The endogenous expressions of related genes were regulated using recombinant plasmids and cell transfection. A Dual-Luciferase Reporter Assay was performed to determine the interaction between miR-34a and STAT3. Rat models with bilateral cavernous nerve injuries (BCNIs) were used to assess erectile function through the detection of mean arterial pressure (MAP) and intracavernosal pressure (ICP). ICA II promoted ADSCs’ proliferation and differentiation to Schwann cells (SCs) through the inhibition of miR-34a. Suppressed miR-34a promoted the differentiation of ADSCs to SCs by up-regulating STAT3. ICA II promoted the differentiation of ADSCs to SCs through the miR-34a/STAT3 pathway. The combination of ICA II and ADSCs preserved the erectile function of the BCNI model rats. ADSCs treated with ICA II markedly preserved the erectile function of the BCNI model rats, which was reversed through miR-34a overexpression. ICA II pro-motes the differentiation of ADSCs to SCs through the miR-34a/STAT3 pathway, contributing to erectile function preservation after the occurrence of a cavernous nerve injury.