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Human HS1BP3 induces cell apoptosis and activates AP-1
( Tai Ping Shi ),( Jie Shi Xie ),( Ying Xiong ),( Wei Wei Deng ),( Jin Hai Guo ),( Feng Wang ),( Da Long Ma ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.6
In the present study, we characterized the function of HS1-binding protein 3 (HS1BP3), which is mutated in essential tremor and may be involved in lymphocyte activation. We found that HS1BP3 localized to the mitochondria and endoplasmic reticulum partially. Overexpression of HS1BP3 induced apoptosis in HEK293T and HeLa cell lines. When these cell lines were transfected with HS1BP3, they exhibited nuclear DNA condensation, externalization of phosphatidylserine (PS), and cleavage of poly ADP ribose polymerase (PARP). Furthermore, suppression of HS1BP3 or HS1 expression attenuates HS1BP3 induced apoptosis. In addition, HS1BP3 enhanced activator protein 1 (AP-1)-mediated transcription in a dose-dependent manner. Therefore, we conclude that HS1BP3 regulates apoptosis via HS1 and stimulates AP-1-mediated transcription. [BMB reports 2011; 44(6): 381-386]
Gui, Tai-Long,Yin, Jing-Hua,Wang, Dong-Xing,Jin, Sung-Ho,Lim, Kwon Taek,Kim, Bong-Shik,Lee, Won-Chul,Gal, Yeong-Soon TaylorFrancis 2007 Molecular Crystals and Liquid Crystals Vol.463 No.1
<P> A new conjugated polymer with bulky substituents was synthesized by the polymerization of ethynylestradiol 3-methyl ether (EEDME) by such transition metal catalysts as PdCl2, RuCl3, and (NBD)PdCl2. The polymerization proceeded well in homogeneous manner to give a moderate yield of polymer. The chemical structure of poly(EEDME) was characterized to have the conjugated polymer backbone with the designed substituents. From the CV measurements, the HOMO energy level of the polymer was calculated to be 5.02 eV. The photoluminescence spectra of poly(EEDME) showed that the two photoluminescence peaks are located at 434 and 406 nm corresponding to the photon energy of 2.86 and 3.06 eV, respectively.</P>
Electro-Optical and Electrochemical Properties of Poly(2-ethynyl-N-glycidylpyridinium bromide)
Gui, Tai-Long,Wang, Yue,Wang, Jian-Min,Jin, Sung-Ho,Shim, Sang-Yeon,Park, Jong-Wook,Lim, Kwon Taek,Gal, Yeong-Soon TaylorFrancis 2009 Molecular Crystals and Liquid Crystals Vol.513 No.1
<P> An ionic polyacetylene with the pendent N-glycidylpyridinium bromide, poly(2-ethynyl-N-glycidylpyridinium bromide), was synthesized in 91% yield by the activated polymerization of 2-ethynylpyridine with epibromohydrin without any additional catalyst or catalyst. This polymer exhibited characteristic UV-visible absorption band at 515 nm and yellow PL spectrum at 598 nm corresponding to the photon energy of 2.07 eV. The cyclovoltamograms of polymer exhibited the electrochemically stable window in the region of -1.4 ∼ 1.8 V and the redox current value gradually increased as the scan rate increased. The kinetics of the redox process of polymer was found to be well-controlled by the reactant diffusion process from the experiment of the oxidation current density of PEGPB versus the scan rate.</P>
대장균에서 발현시킨 사람 파필로마 바이러스 18 형 E6 단백질의 분리정제와 사람 혈청내의 18 형 E6 항체 검색에의 이용
정태화,손우익,박순희,강주현,유왕돈,진승원 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Human papillomavirus type 16 and 18 encode two major transforming proteins E6 and E7. Recombinant E6 protein for human papillomavirus type 18(HPV 18) E6 gene was expressed in E. coli in large quantity as authentic size, non fused form of protein. However, the protein was produced in inclusion bodies. Therefore this bacterially produced E6 protein was solublized in SDS solution and purified to the homogeneity by gel filtration column chromatographies using Sephacryl S-200 column. The yield of purified 18 E6 recombinant protein was approximately 30 ㎎/ℓ liter culture. This purified recombinant 18E6 protein was used to determine the presence of antibodies that might have been produced against HPV 18 infections in the human sera. We tested and compared sera obtained from both cervical cancer patients and people having no apparent cervical cancers. These were accomplished using Western blot. Antibodies reactive to recombinant HPV 18E6 protein were frequently detected in cases with cervical carcinoma(14 out of 20, 70%), compared to people with no apparent carcinoma(4 out of 9, 44%).
Zhi-gang Tai,Yi-ren Zhu,Yi-bo Yuan,Jin Liu,Zhen-jie Li,Zhi-hua Liu,Kun-miao Wang 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.3
In this work, a highly sensitive method using a colorimetric probe coupled to dispersive liquid?liquid microextraction (DLLME) was developed for the quantitative determination of dopamine (DA) in serum. The DA in serum was concentrated by DLLME to increase the detection sensitivity and reduce the matrix effects. After the DLLME process, a colorimetric probe of silver triangular nanoparticles (AgTNPs) was used to detect DA, which was based on the plasma transformation of AgTNPs caused by strong interactions with melamine (MA). The results showed that DA could inhibit the aggregation of AgTNPs induced by MA, resulting in the recovery of the surface plasmon resonance (SPR) peak of AgTNPs. Thus, the DLLME method followed by colorimetric probe detection of DA can be achieved. The parameters affecting the proposed method were optimized, under the optimal conditions, a linear calibration curve was obtained over a concentration range of 5 to 250?nM with a recovery from 94.4 to 101.3%. The detection limit was 1.6 nM (at an S/N ratio of 3). The present method was successfully applied to determine DA in human serum.
Feng, Enguang,Shin, Woo-Jin,Zhu, Xuelian,Li, Jian,Ye, Deju,Wang, Jiang,Zheng, Mingyue,Zuo, Jian-Ping,No, Kyoung Tai,Liu, Xian,Zhu, Weiliang,Tang, Wei,Seong, Baik-Lin,Jiang, Hualiang,Liu, Hong American Chemical Society 2013 Journal of medicinal chemistry Vol.56 No.3
<P>In order to exploit the 430-cavity in the active sites of neuraminidases, 22 zanamivir analogs with C-1 and C-4 modification were synthesized, and their inhibitory activities against both group-1 (H5N1, H1N1) and group-2 neuraminidases (H3N2) were determined. Compound <B>9f</B> exerts the most potency, with IC<SUB>50</SUB> value of 0.013, 0.001, and 0.09 μM against H3N2, H5N1, and H1N1, which is similar to that of zanamivir (H3N2 IC<SUB>50</SUB> = 0.0014 μM, H5N1 IC<SUB>50</SUB> = 0.012 μM, H1N1 IC<SUB>50</SUB> = 0.001 μM). Pharmacokinetic studies of compound <B>9f</B> in rats showed a much longer plasma half-life (<I>t</I><SUB>1/2</SUB>) than that of zanamivir following administration (po dose). Molecular modeling provided information about the binding model between the new inhibitors and neuraminidase, with the elongated groups at the C-1-position being projected toward the 430-loop region. This study may represent a novel starting point for the future development of improved antiflu agents.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jmcmar/2013/jmcmar.2013.56.issue-3/jm3009713/production/images/medium/jm-2012-009713_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jm3009713'>ACS Electronic Supporting Info</A></P>
왕승진,장태우,김호연,남윤석,Wang, Seung-Jin,Chang, Tai-Woo,Kim, Ho-Yon,Nam, Yun-Seok 한국정보처리학회 2004 정보처리학회논문지D Vol.11 No.3
현재 우리나라의 인만 서장우편 물량은 매년 증가하고 있는 추세이며 특히 월말이나 연말에 집중화되는 계절성과 순환성의 특성을 보인다. 그러나 우편물량의 증가와 월말 또는 연말에 집중화되는 현상에 적절히 대응하지 못하고 있으며, 집배원의 인력 부족으로 서비스의 질적 저하와 업무 부하량 증가 등의 문제가 더욱 가시화되어 가고 있다. 이러한 상황에서 신속한 우편물 처리와 집배원의 업무 경감 등을 위해 배달우편물 순로구분 시스템, 주소정보 시스템 도입 등의 노력이 시도되고 있으나. 일반 소비자 또는 다량 우편물 발송업체 등의 주소 기재 방식이 일정하지 않고 표현 방법이 다양하여 자동화 기계 및 집배원 업무효율의 저하가 예상되고 있는 실정이다 따라서 본 논문에서는 우편물에 기입하는 주소의 표기 표준화를 통해 주소인식률과 집배원의 배달 구분 업무 효율성을 향상시키고자 한다. 먼저 국내 우편주소 체계와 정보 구조를 살펴보고, 각각의 문제점을 파악하여 표준화 시 고려할 요소들을 표기 내용을 중심으로 정리하였다. 그리고 자동주소인식을 위해 다양한 형식의 결정을 위해 고려사항들을 제시하였다. Currently the amount of general letter mail in Korea is on an increasing trend; especially it shows the seasonality that is concentrated in the end of the month or the year. However, the situation is not treated appropriately Moreover. manpower insufficiency if the carriers causes the poor service and the heavy burden of work. Under the existing conditions, the various efforts such as development of the automatic sequence sorting system and construction of the address database are given in order to quicken mall process and lighten the carriers' burden But the inconsistent addressing and the various expressions of address drop the systems and the carriers off in efficiency. In this study, we present the Korean postal addressing standards to make it possible to improve the performance of address recognition and help postmen to sort the mail; particularly focusing on the substance. After analyzing the domestic system, the information structure and the problems of postal address, we propose four fundamentals and the standards including synonyms and acronyms. In addition, we suggest several considerations for mail format. We expect that this study could support the postal service in Korea as a basic standard.
Park, Ju Hyun,Wang, Zesong,Jeong, Hee-Jin,Park, Hee Ho,Kim, Byung-Gee,Tan, Wen-Song,Choi, Shin Sik,Park, Tai Hyun Springer International 2012 Applied microbiology and biotechnology Vol.96 No.3
<P>We previously reported that the expression of Bombyx mori 30Kc19 gene in CHO cells significantly improved both the production and sialylation of recombinant human EPO (rHuEPO) in adhesion culture mode. In this study, the effects of 30Kc19 expression and supplementation of 30Kc19 recombinant protein on the productivity and glycosylation pattern of rHuEPO were investigated in the serum-free suspension culture mode. Especially, glycosylation pattern was examined in detail using a quantitative MALDI-TOF MS method. The expression of 30Kc19 increased the EPO production by 2.5-folds and the host cells produced rHuEPO with more complex glycan structures and a larger content of sialic acid and fucose. The glycan structures of rHuEPO in the 30Kc19-expressing cell consisted of bi-, tri-, tetra-, and penta-antennary branching (35, 18, 33, and 14 %, respectively), while the control cells produced predominantly bi-antennary branching (70 %). About 53 % of the glycans from rHuEPO in the 30Kc19-expressing cell was terminally sialylated, while no obvious sialylated glycan was found in the control cells. The percentage of fucosylated glycans from the 30Kc19-expressing cell culture was 77 %, whereas only 61 % of the glycans from the control cell were fucosylated glycans. We also examined whether these effects were observed when the recombinant 30Kc19 protein produced from Escherichia coli was supplemented into the culture medium for CHO cells. In the control cell line without the 30Kc19 gene, EPO production increased by 41.6 % after the addition of 0.2 mg/mL of the recombinant 30Kc19 protein to the culture medium. By the Western blot analysis after two-dimensional electrophoresis (2-DE) of isoforms of EPO, we confirmed that 30Kc19 enhanced the sialylation of EPO glycans. These results demonstrated that both 30Kc19 gene expression and the recombinant 30Kc19 protein addition enhanced rHuEPO productivity and glycosylation in suspension culture. In conclusion, the utilization of 30Kc19 in CHO cell culture holds great promise for use in the manufacturing of improved biopharmaceutical glycoproteins.</P>