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Taewoan Koo,Seung Gu Shin,Joonyeob Lee,Seokhwan Hwang 한국폐기물자원순환학회 2015 한국폐기물자원순환학회 학술대회 Vol.2015 No.11
Methanogenic community shift and comparison were determined by 454 pryosequencing for two different full-scale anaerobic digesters treating municipal sludge. For monitoring long-term of microbial communities, samples were collected for two year at three-monthly basis. The two mesophilic AD bioreactor were operated at similar operating conditions, but different substrate streams. Methanospirillum were identified as the key drivers of methanogenesis in full-scale anaerobic digester treating municipal sludge. In Joongrang (JR) digester, Methanospirillum was dominant (48%±10.3) over almost all period, but the dominant genus move to Methanosaeta and Methanoculleus due to low acetate concentration (0.02 g/L), total ammonia nitrogen concentration, respectively. In Asan digester (AS), Methanospirillum also was dominant (41%±12.6) like JR digester, but methanogenic community shift was examined twice. One of those was from Methanospirillum to Methanophaerula due to pH sharply decrease (<5.5) and second shift was Methanosaeta increase due to low VFAs concentration (0.25 g/L).
( Taewoan Koo ),( Hyokwan Bae ),( Joonyeob Lee ),( Kyungjin Cho ),( Jangwoo Lee ),( Seokhwan Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.2
Agricultural wastes including starch-processing waste have no toxic compounds and their major constituents are carbohydrate and protein except water. Newly, nutrients recovery by bioconversion in the starch-processing waste is viewed as a considerable approach since it produces valuable product as well as wastewater treatment. The effects of pH, temperature and substrate concentration on mycelia growth rate of Inonotus obliquus in the solid state fermentation was investigated separately using response surface methodology (RSM). Central composite design (CCD) was applied to optimize the operating variables. The obtained experimental datas were fitted to a quadratic polynomial equation using multiple regression analysis and also analysed by appropriate statistical methods to verify model performance. A mathematical model was then developed to show the effects of each variables and their interactions on the mycelia growth rate. The substrate concentration range of Inonotus obliquus determined 40g/l~60g/l for the present experiment was based on the results of statistical analysis by substrate inhibition Shi model. The others independent range of Inonotus obliquus were pH 4.5~6.5, 20~30℃. The optimal conditions for mycelia growth rate was for the cultured mycelium of Inonotus obliquus was determined, analysing three dimensional surface plot and contour plot, as substrate concentration 51.2 g/l, pH 6.50, temperature 23.4℃ and under these optimum conditions, the maximum growth rate was 4.20 mm/day.
( Taewoan Koo ),( Seung Gu Shin ),( Joonyeob Lee ),( Seokhwan Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 심포지움 Vol.2015 No.2
Methanogenic community shift and comparison were determined by 454 pryosequencing for two different full-scale anaerobic digesters treating municipal sludge. For monitoring long-term of microbial communities, samples were collected for two year at three-monthly basis. The two mesophilic AD bioreactor were operated at similar operating conditions, but different substrate streams. Methanospirillum were identified as the key drivers of methanogenesis in full-scale anaerobic digester treating municipal sludge. In Joongrang (JR) digester, Methanospirillum was dominant (48%±10.3) over almost all period, but the dominant genus move to Methanosaeta and Methanoculleus due to low acetate concentration (0.02 g/L), total ammonia nitrogen concentration, respectively. In Asan digester (AS), Methanospirillum also was dominant (41%±12.6) like JR digester, but methanogenic community shift was examined twice. One of those was from Methanospirillum to Methanophaerula due to pH sharply decrease (<5.5) and second shift was Methanosaeta increase due to low VFAs concentration (0.25 g/L).
( Taewoan Koo ),( Seung Gu Shin ),( Joonyeob Lee ),( Seokhwan Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 추계학술발표논문집 Vol.2015 No.-
Methanogenic community shift and comparison were determined by 454 pryosequencing for two different full-scale anaerobic digesters treating municipal sludge. For monitoring long-term of microbial communities, samples were collected for two year at three-monthly basis. The two mesophilic AD bioreactor were operated at similar operating conditions, but different substrate streams. Methanospirillum were identified as the key drivers of methanogenesis in full-scale anaerobic digester treating municipal sludge. In Joongrang (JR) digester, Methanospirillum was dominant (48%±10.3) over almost all period, but the dominant genus move to Methanosaeta and Methanoculleus due to low acetate concentration (0.02 g/L), total ammonia nitrogen concentration, respectively. In Asan digester (AS), Methanospirillum also was dominant (41%±12.6) like JR digester, but methanogenic community shift was examined twice. One of those was from Methanospirillum to Methanophaerula due to pH sharply decrease (<5.5) and second shift was Methanosaeta increase due to low VFAs concentration (0.25 g/L). Acknowledgements: This work was supported by Korea Ministry of Environment as “Human Resource Development Project for Energy from Waste & Recycling.” 본 연구는 환경부의 지식기반 환경서비스(폐자원에너지화) 전문인력 양성사업으로 지원되었습니다.
김태완(Taewoan Kim),김완찬(Wanchan Kim),고영성(Youngsung Ko),김선진(Sunjin Kim),한영민(Yeoungmin Han) 한국추진공학회 2014 한국추진공학회 학술대회논문집 Vol.2014 No.12
본 연구에서는 500N급 소형 액체로켓엔진을 사용하여, 약 25㎞(0.025bara) 고도의 대기압 환경을 조성할 수 있는 초음속 디퓨저와 이젝터 조합의 고공시험 설비를 구축하였으며, 설비의 성능 검증 차원에서 축소형 액체로켓엔진 고공환경 모사시험을 수행하였다. 시험 설비는 고공환경 모사장치와 추진제공급설비 그리고 냉각수 공급설비로 구성된다. 본 고공시험 설비로 약 27㎞(0.021bara) 고도에 해당하는 대기 압력을 성공적으로 구현하였으며, 이때 축소형 액체로켓엔진에서 발생하는 추력 성능을 확인하였다. A high altitude test facility which includes supersonic diffuser and ejector has been developed to simulate atmospheric pressure at 25㎞ using a 500N class small liquid rocket engine. Also high altitude simulation test for the small liquid rocket engine was performed to verify its performance. The experimental facility consists of high altitude simulation device, propellants supply system and coolant supply system. Low pressure condition corresponding to about 27㎞(0.021bar) altitude atmosphere was simulated successfully and a small liquid rocket engine thrust level was confirmed at the low pressure condition by the high altitude test facility through verification test.
Koo, Taewoan,Lee, Joonyeob,Hwang, Seokhwan Academic Press 2019 Journal of Environmental Management Vol. No.
<P><B>Abstract</B></P> <P>The specific primer and probe sets for quantifying <I>Clostridium cadaveris</I> and <I>Clostridium sporogenes</I> using a quantitative real-time PCR were designed. Each primer and probe set detected only the target species very specifically. The two species were cultivated in pure and mixed culture in batch mode with glucose as the only carbon source. The designed QPCR sets were used successfully to estimate the biokinetic parameters of each species in pure culture: i.e., maximum specific growth rate <I>μ</I> <SUB>max</SUB>, half saturation concentration <I>K</I> <SUB> <I>s</I> </SUB>, growth yield <I>Y</I>, and decay coefficient <I>K</I> <SUB> <I>d</I> </SUB>. of <I>C. cadaveris</I> and <I>C. sporogenes</I> were 0.311 ± 0.020 and 0.360 ± 0.019 h<SUP>-1</SUP>, 4.241 ± 1.653 and 5.171 ± 1.097 g/L, 0.301 ± 0.065 and 0.199 ± 0.037 10<SUP>11</SUP> copies/g, 0.005 ± 0.043 and 0.009 ± 0.025 h<SUP>-1</SUP>, respectively. The effect of interspecific interaction of on substrate consumption rate and microbial growth was evaluated using mixed culture; curve fitting and comparison of coefficients detected increase in substrate consumption rate but decrease in microbial growth rate; these results imply interspecific interaction effect. A new model was of the interspecific interaction was developed, with focus on accuracy, realism, simplicity and biological significance. This interspecific interaction model may be extended to more-complex bioprocesses such as biological wastewater treatment systems and anaerobic digestion.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The real-time PCR primer and probe sets of <I>C. cadaveris</I> and <I>C. sporogenes</I> were designed. </LI> <LI> Biokinetic parameters derived from pure culture had statistically significant values. </LI> <LI> <I>Cadaveris</I> and <I>C. sporogenes</I> were cultivated together to prove interactions effect. </LI> <LI> Substrate consumption rate increased, while microbial growth rate decreased by interaction. </LI> <LI> The novel interaction model was developed to quantify the interspecific interaction. </LI> </UL> </P>
김태완(Taewoan Kim),전준수(Junsu Jeon),김완찬(Wanchan Kim),고영성(Youngsung Ko),김선진(Sunjin Kim),한영민(Youngmin Han) 한국추진공학회 2014 한국추진공학회 학술대회논문집 Vol.2014 No.5
본 논문은 현재 충남대에서 수행중인 액체로켓엔진 고공환경 모사 시험 연구에 대한 진행 현황을 기술하였다. 액체로켓엔진 고공환경 모사 시험 설비는 고공용 로켓 엔진, 이차목 초음속 디퓨저, 열교환기, 스팀제너레이터, 이젝터로 구성된다. 현재 모든 구성 설비의 제작이 완료되어 파트별 검증실험을 진행 중에 있으며, 고공용 로켓 엔진 연소시험과 스팀제너레이터 작동 시험 그리고 디퓨저/열교환기의 벽면냉각을 위한 냉각수 공급 시험을 수행하였다. This paper describes the development status of high altitude test facility(HATF) construction for liquid rocket engine which was executed by Chung-nam National University. The experimental facility consists of vacuum chamber, secondary throat supersonic diffuser, heat exchanger, steam generator and ejector. All the parts are undergoing verification tests respectively in order to confirm characteristics after manufacturing. Liquid rocket engine combustion test, steam generator operation test and water supply tests of diffuser/heat exchanger were performed step by step.