http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Taewoan Koo,Seung Gu Shin,Joonyeob Lee,Seokhwan Hwang 한국폐기물자원순환학회 2015 한국폐기물자원순환학회 학술대회 Vol.2015 No.11
Methanogenic community shift and comparison were determined by 454 pryosequencing for two different full-scale anaerobic digesters treating municipal sludge. For monitoring long-term of microbial communities, samples were collected for two year at three-monthly basis. The two mesophilic AD bioreactor were operated at similar operating conditions, but different substrate streams. Methanospirillum were identified as the key drivers of methanogenesis in full-scale anaerobic digester treating municipal sludge. In Joongrang (JR) digester, Methanospirillum was dominant (48%±10.3) over almost all period, but the dominant genus move to Methanosaeta and Methanoculleus due to low acetate concentration (0.02 g/L), total ammonia nitrogen concentration, respectively. In Asan digester (AS), Methanospirillum also was dominant (41%±12.6) like JR digester, but methanogenic community shift was examined twice. One of those was from Methanospirillum to Methanophaerula due to pH sharply decrease (<5.5) and second shift was Methanosaeta increase due to low VFAs concentration (0.25 g/L).
( Taewoan Koo ),( Hyokwan Bae ),( Joonyeob Lee ),( Kyungjin Cho ),( Jangwoo Lee ),( Seokhwan Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.2
Agricultural wastes including starch-processing waste have no toxic compounds and their major constituents are carbohydrate and protein except water. Newly, nutrients recovery by bioconversion in the starch-processing waste is viewed as a considerable approach since it produces valuable product as well as wastewater treatment. The effects of pH, temperature and substrate concentration on mycelia growth rate of Inonotus obliquus in the solid state fermentation was investigated separately using response surface methodology (RSM). Central composite design (CCD) was applied to optimize the operating variables. The obtained experimental datas were fitted to a quadratic polynomial equation using multiple regression analysis and also analysed by appropriate statistical methods to verify model performance. A mathematical model was then developed to show the effects of each variables and their interactions on the mycelia growth rate. The substrate concentration range of Inonotus obliquus determined 40g/l~60g/l for the present experiment was based on the results of statistical analysis by substrate inhibition Shi model. The others independent range of Inonotus obliquus were pH 4.5~6.5, 20~30℃. The optimal conditions for mycelia growth rate was for the cultured mycelium of Inonotus obliquus was determined, analysing three dimensional surface plot and contour plot, as substrate concentration 51.2 g/l, pH 6.50, temperature 23.4℃ and under these optimum conditions, the maximum growth rate was 4.20 mm/day.
( Taewoan Koo ),( Seung Gu Shin ),( Joonyeob Lee ),( Seokhwan Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 심포지움 Vol.2015 No.2
Methanogenic community shift and comparison were determined by 454 pryosequencing for two different full-scale anaerobic digesters treating municipal sludge. For monitoring long-term of microbial communities, samples were collected for two year at three-monthly basis. The two mesophilic AD bioreactor were operated at similar operating conditions, but different substrate streams. Methanospirillum were identified as the key drivers of methanogenesis in full-scale anaerobic digester treating municipal sludge. In Joongrang (JR) digester, Methanospirillum was dominant (48%±10.3) over almost all period, but the dominant genus move to Methanosaeta and Methanoculleus due to low acetate concentration (0.02 g/L), total ammonia nitrogen concentration, respectively. In Asan digester (AS), Methanospirillum also was dominant (41%±12.6) like JR digester, but methanogenic community shift was examined twice. One of those was from Methanospirillum to Methanophaerula due to pH sharply decrease (<5.5) and second shift was Methanosaeta increase due to low VFAs concentration (0.25 g/L).
( Taewoan Koo ),( Seung Gu Shin ),( Joonyeob Lee ),( Seokhwan Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 추계학술발표논문집 Vol.2015 No.-
Methanogenic community shift and comparison were determined by 454 pryosequencing for two different full-scale anaerobic digesters treating municipal sludge. For monitoring long-term of microbial communities, samples were collected for two year at three-monthly basis. The two mesophilic AD bioreactor were operated at similar operating conditions, but different substrate streams. Methanospirillum were identified as the key drivers of methanogenesis in full-scale anaerobic digester treating municipal sludge. In Joongrang (JR) digester, Methanospirillum was dominant (48%±10.3) over almost all period, but the dominant genus move to Methanosaeta and Methanoculleus due to low acetate concentration (0.02 g/L), total ammonia nitrogen concentration, respectively. In Asan digester (AS), Methanospirillum also was dominant (41%±12.6) like JR digester, but methanogenic community shift was examined twice. One of those was from Methanospirillum to Methanophaerula due to pH sharply decrease (<5.5) and second shift was Methanosaeta increase due to low VFAs concentration (0.25 g/L). Acknowledgements: This work was supported by Korea Ministry of Environment as “Human Resource Development Project for Energy from Waste & Recycling.” 본 연구는 환경부의 지식기반 환경서비스(폐자원에너지화) 전문인력 양성사업으로 지원되었습니다.
Koo, Taewoan,Shin, Seung Gu,Lee, Joonyeob,Han, Gyuseong,Kim, Woong,Cho, Kyungjin,Hwang, Seokhwan Elsevier 2017 Bioresource technology Vol.228 No.-
<P><B>Abstract</B></P> <P>Four full-scale mesophilic anaerobic digesters treating waste sludge were monitored to characterize methanogen communities and their relationship with process parameters. The performance of the four digesters were dissimilar with the average chemical oxygen demand removal efficiencies between 24 and 45% and differing pH. Real-time quantitative PCR showed that archaeal 16S rRNA gene concentration ([ARC]) and, more pronouncedly, its ratio to bacterial counterpart ([ARC]/[BAC]) correlated positively with the performance parameters, including the lipid removal efficiency. Pyrosequencing identified 12 methanogen genera, of which <I>Methanolinea, Methansaeta</I>, and <I>Methanospirillum</I> collectively accounted for 79.2% of total archaeal reads. However, <I>Methanoculleus</I>, a numerically minor (1.9±2.6%) taxa, was the most promising biomarker for positive performance, while <I>Methanoregula</I> was abundant in samples with poor performance. These results could be useful for the control and management of anaerobic sludge digestion.</P> <P><B>Highlights</B></P> <P> <UL> <LI> [ARC] and [ARC]/[BAC] positively correlated with process performance parameters. </LI> <LI> Most abundant methanogens did not show clear correlation with performance parameters. </LI> <LI> <I>Methanoculleus</I> was potentially a promising biomarker for positive performance. </LI> <LI> <I>Methanoregula</I> was abundant in samples with poor performance. </LI> </UL> </P>
Koo, Taewoan,Lee, Joonyeob,Hwang, Seokhwan Academic Press 2019 Journal of Environmental Management Vol. No.
<P><B>Abstract</B></P> <P>The specific primer and probe sets for quantifying <I>Clostridium cadaveris</I> and <I>Clostridium sporogenes</I> using a quantitative real-time PCR were designed. Each primer and probe set detected only the target species very specifically. The two species were cultivated in pure and mixed culture in batch mode with glucose as the only carbon source. The designed QPCR sets were used successfully to estimate the biokinetic parameters of each species in pure culture: i.e., maximum specific growth rate <I>μ</I> <SUB>max</SUB>, half saturation concentration <I>K</I> <SUB> <I>s</I> </SUB>, growth yield <I>Y</I>, and decay coefficient <I>K</I> <SUB> <I>d</I> </SUB>. of <I>C. cadaveris</I> and <I>C. sporogenes</I> were 0.311 ± 0.020 and 0.360 ± 0.019 h<SUP>-1</SUP>, 4.241 ± 1.653 and 5.171 ± 1.097 g/L, 0.301 ± 0.065 and 0.199 ± 0.037 10<SUP>11</SUP> copies/g, 0.005 ± 0.043 and 0.009 ± 0.025 h<SUP>-1</SUP>, respectively. The effect of interspecific interaction of on substrate consumption rate and microbial growth was evaluated using mixed culture; curve fitting and comparison of coefficients detected increase in substrate consumption rate but decrease in microbial growth rate; these results imply interspecific interaction effect. A new model was of the interspecific interaction was developed, with focus on accuracy, realism, simplicity and biological significance. This interspecific interaction model may be extended to more-complex bioprocesses such as biological wastewater treatment systems and anaerobic digestion.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The real-time PCR primer and probe sets of <I>C. cadaveris</I> and <I>C. sporogenes</I> were designed. </LI> <LI> Biokinetic parameters derived from pure culture had statistically significant values. </LI> <LI> <I>Cadaveris</I> and <I>C. sporogenes</I> were cultivated together to prove interactions effect. </LI> <LI> Substrate consumption rate increased, while microbial growth rate decreased by interaction. </LI> <LI> The novel interaction model was developed to quantify the interspecific interaction. </LI> </UL> </P>
Lee, Joonyeob,Koo, Taewoan,Yulisa, Arma,Hwang, Seokhwan Academic Press 2019 Journal of Environmental Management Vol. No.
<P><B>Abstract</B></P> <P>Anaerobic batch tests with a 2<SUP>2</SUP> full-factorial design of ammonia (1.5, 6.5 g N/L) and magnetite concentrations (0, 20 mmol/L) were conducted separately for methanogenic degradation of acetate, propionate, and butyrate (volatile fatty acids (VFAs)) to 1) quantify the effect of magnetite as an enhancer in methanogenic degradation of each of the VFAs in conditions without ammonia stress (1.5 g N/L) and with ammonia stress (6.5 g N/L), and 2) identify methanogenic consortia that are related to such enhancement. Among the three VFAs, methanogenic degradation of propionate was the least feasible (57% lower specific methanogenic activity <I>R</I> <SUB> <I>CH4</I> </SUB> and three times longer lag time <I>λ</I> than acetate degradation). At low ammonia concentration, only propionate showed improvement in <I>R</I> <SUB> <I>CH4</I> </SUB> (46%) with supplementation of magnetite. In the ammonia-stressed condition without magnetite, <I>R</I> <SUB> <I>CH4</I> </SUB> decreased by 38–58% and <I>λ</I> increased 2.2–8.8 times for all VFAs; magnetite supplementation significantly alleviated these effects. These results demonstrate that magnetite supplementation effectively increases methanogenic degradation of the VFAs even under ammonia-stressed conditions. 16S metagenomic sequencing revealed that distinctive methanogenic consortia were active in the different combinations of substrate, ammonia and magnetite. <I>Alkaliphilus</I>, <I>Hyphomonadaceae SWB02</I> and <I>Clostridia DTU014</I>, <I>Clostridia D8A-2</I>, <I>Christensenellaceae R-7 group</I> and <I>Rikenellaceae DMER64</I> were identified as potential syntrophic bacteria that can establish magnetite-mediated direct electron transfer with methanogens (<I>Methanosaeta concilii</I>, <I>Methanosaeta harundinacea</I>, <I>Methanolinea tarda</I>, <I>Methanoculleus bourgensis</I> and <I>Methanosarcina</I> spp.) during methanogenic degradation of VFAs. The results may be useful as a reference to develop effective strategies using magnetite supplementation to remediate anaerobic digestion processes that have been afflicted by VFA accumulation and ammonia inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Magnetite effect on AD of VFAs under NH<SUB>3</SUB>-stressed condition was tested. </LI> <LI> Methanogenic degradation of propionate was improved by magnetite addition. </LI> <LI> Magnetite alleviated NH<SUB>3</SUB> inhibition of methanogenic degradation of VFAs. </LI> <LI> Distinctive syntrophic consortia were responsible for such enhancement effect. </LI> </UL> </P>