Evaluation of a new flow cytometry based method for detection of BCR-ABL1 fusion protein in chronic myeloid leukemia

Swati Dasgupta,Ujjal K Ray,Arpita Ghosh Mitra,Deboshree M. Bhattacharyya,Ashis Mukhopadhyay,Priyabrata Das,Sudeshna Gangopadhyay,Sudip Roy,Soma Mukhopadhyay 대한혈액학회 2017 Blood Research Vol.51 No.2

Background: Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitor-ing of CML. Previously, many technologies, most of which are laborious and time consum-ing, have been developed to detect BCR-ABL chimeric gene or chromosome. Methods: A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion pro-teins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. Results: From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantita-tive real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. Conclusion: BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.

Evaluation of a new flow cytometry based method for detection of BCR-ABL1 fusion protein in chronic myeloid leukemia

Swati Dasgupta,Ujjal K Ray,Arpita Ghosh Mitra,Deboshree M. Bhattacharyya,Ashis Mukhopadhyay,Priyabrata Das,Sudeshna Gangopadhyay,Sudip Roy,Soma Mukhopadhyay 대한혈액학회 2017 Blood Research Vol.52 No.2

Background: Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitor-ing of CML. Previously, many technologies, most of which are laborious and time consum-ing, have been developed to detect BCR-ABL chimeric gene or chromosome. Methods: A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion pro-teins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. Results: From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantita-tive real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. Conclusion: BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.

Pulsatile flow of blood with shear-dependent viscosity through a flexible stenosed artery in the presence of body acceleration

Subrata Mukhopadhyay,Mani Shankar Mandal,Swati Mukhopadhyay 한국유변학회 2022 Korea-Australia rheology journal Vol.34 No.1

A mathematical model of physiological pulsatile fl ow of blood through a stenotic flexible artery in the presence of body acceleration is presented in this paper. Streaming blood is considered as a shear-thinning non-Newtonian fluid as proposed by Yeleswarapu (Evaluation of continuum models for characterizing the constitutive behaviour of blood, Ph.D. thesis, Dept. Mech. Eng., University of Pittsburgh, 1996), and a physiological pulsatile fl ow rate proposed by Pedrizzetti (J Fluid Mech 310:89–111, 1996) has been taken through the tube. Deformation of vessel wall is modelled as a function of fl ow rate. This computational study of an idealized model may bring some insights for realistic blood fl ow through a stenotic artery. The novelty of this work lies in the fact that realistic fl ow of blood through a stenosed artery has been studied as far as possible and a new idea has been provided to describe the arterial wall motion. Governing equations in cylindrical polar coordinates are solved using stream function–vorticity method. Behaviour of various fl ow quantities is investigated through a parametric study. It is noted that the degree of constriction and body acceleration have important impacts on the haemodynamic parameters such as wall shear stress, oscillatory shear index, and relative residence time. Increasing body acceleration enhances the peak value of wall shear stress, but reduces the oscillatory shear index and relative residence time. Almost 1/4th increase in length of fl ow separation is found when Froude number raises its value from 0.1 to 0.5, other parametric values remaining fixed. On the other hand, almost 50% increase in the magnitude of the peak value of wall pressure is found when the amplitude of body acceleration takes a value 0.4 ( A = 0.4) compared to the without body acceleration case ( A = 0). These results have a significant role.

Rate parameter estimation for 4-chlorophenol degradation by UV and organic oxidants

Swati Sharma,Mausumi Mukhopadhyay,Z.V.P. Murthy 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.1

The main objective of this study was to evaluate the rate parameters of degradation/mineralization of 4-chlorophenol (4-CP) using organic oxidants, viz., peroxy acetic acid (PAA), para nitro benzoic acid (PNBA),and methyl ethyl ketone peroxide (MEKP), in basic media, in combination with UV irradiation at 254 nm. All the experiments were conducted with varying time intervals, under identical conditions of UV irradiation and pH. The reactions were followed by HPLC analysis for residual concentration and GC–MS for mineralization, and intermediates/product identification. In the present investigation, the residual concentration data of 4-CP against time were analyzed for reaction order determination by differential and integral methods. The differential method yielded fractional order of reaction for all organic oxidants under consideration. Initially the profile was observed to be linear in the time span of 0–45 s for all the organic oxidants used and thus, first-order approximation was applied. The curvilinear path, which followed up till 300 s, was approximated to first-order and second-order kinetics. No noteworthy results were observed for pseudo-first order rate equation. The change in the nature of the curves was attributed to the formation of intermediates/reaction products.

A Randomized Controlled Trial to Compare the Efficacy, Safety and Tolerability of Asenapine versus Olanzapine in Management of Schizophrenia

Arpita Maitra,Swati Bhattacharyya,Sabyasachi Mukhopadhyay,Asim Kumar Mallick,Supreeti Biswas,Om Prakash Singh 대한정신약물학회 2020 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.18 No.4

Objective: Schizophrenia is a serious disease characterized by impairment in the perception or expression of reality, leading to occupational and social dysfunction. The use of antipsychotic medication is now universal in the first-line treatment of schizophrenia. This study was undertaken to compare the efficacy of asenapine with a standard atypical antipsychotic, olanzapine in treating this disease. Methods: It was designed as a single blind, randomized, controlled, parallel group, single centre Phase IV trial of a newer atypical antipsychotic, asenapine versus existing standard atypical antipsychotic, olanzapine. Total 80 subjects were enrolled as per eligibility criteria.Each recruited subject received daily treatment with the trial medication (Olanzapine 10 mg or Asenapine 10 mg daily) for duration of 12 weeks. BPRS, CGI-S, CGI-I, Laboratory parameters and compliance was assessed and analyzed. Continuous variables were compared by t test and non-parametric data was analyzed by Mann−Whitney U test and Wilcoxon signed rank test. Likely categorical variables were analyzed by chi-square test or Fisher’s exact test, as appropriate. Results: The duration of schizophrenia at presentation was comparable in both the treatment groups. There was significant reduction of BPRS score between any two visits of each treatment groups. The decline in CGI-S and CGI-I scores was statistically significant (p < 0.001) when compared between visits of any of the both treatment arms. Adherence to treatment was excellent for all patients. Conclusion: Newer atypical antipsychotic asenapine is more effective than standard olanzapine in reducing the symptoms of schizophrenia in this study and further larger studies are to be done.