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Diamagnetic Microparticle Movement in High Gradient Magnetic Separation Analyses
Svetlana B. Norina,Kwang-Sup Soh,Sang-Hyun Park 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.1
Diamagnetic capture of red blood cells and Bonghan granules near a magnetized ferromagnetic wire was studied with high gradient magnetic collection parametric measurements. The diamagnetic trajectories followed the analytical equations for the axial configuration with a vertically oriented wire. Single erythrocytes of normal and diseased donors were tested in capture measurements, and a considerable difference resulted in the magnetic velocities. The magnetic susceptibility of Bonghan granules was measured in the buildup process on the wire under stopped-flow conditions. The buildup profiles of the accumulation process for super-paramagnetic submicron particles of ferritin and for diamagnetic DNA-containing Bonghan granules were compared using the developed simulation model of separated droplet growth. The diamagnetic values of the magnetic susceptibility for Bonghan granules were evaluated by using several methods, and the values varied widely:−(0.8 5.8) × 10−8 (CGS units).
Svetlana B. Norina 한국물리학회 2007 Current Applied Physics Vol.7 No.4
separation (magnetophoresis). Nucleoprotein granules separated from cultivated connective tissue and nuclei of chicken red blood cellsformed various chromatin mesophases and chiral supra-molecular structures. The applied magnetic pressure accelerated mesophase for-mations due to the diamagnetic alignment and the concentrating of nucleosome nanoparticles and aggregates (germs) in gradient mag-netic elds. Under various magnetic force parameters (H ÆgradH). 1051011 Oe2/cm the dierent mesophases emerged. Chickennucleated red blood cells were found to be a convenient model to study the chromatin condensation under inuences including electro-magnetic factors.
Norina, Svetlana B.,Kim, Jungdae,Soh, Kwang-Sup IOS PRESS B.V 2007 INTERNATIONAL JOURNAL OF APPLIED ELECTROMAGNETICS Vol.25 No.1-4
<P>A magnetic capture of chromatin particles, ferritin, bacterial cells and shrimps was effectively used to measure the magnetic susceptibility, Fourier-spectra, and real-time graphs. In the high gradient magnetic separation (HGMS) using the low-frequency photo-detection the new dynamical bio-particle parameters were obtained. The concentration phase transitions and the diamagnetic alignment were revealed for colloid suspensions of the chromatin particles and the nuclei of chicken red blood cells in the HGMS. A biosensor detecting dynamical changes in the refractive index using a spectroscopy of intensity fluctuations was combined with the HGMS to detect a diamagnetic and paramagnetic capture, liquid-crystalline phase transitions in DNA containing particles, a dynamical autofluorescence and frequency spectra. Chicken red blood cell nuclei formed liquid-crystalline very bright (cholesteric) tubular-hexagonal structures and networks under their diamagnetic alignment in high gradient magnetic fields.</P>
Norina, Svetlana B.,Park, Sang-Hyun,Kim, Jungdae,Cho, Sungil,Shalygin, A. N.,Soh, Kwang-Sup SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS 2005 JOURNAL OF BIOMEDICAL OPTICS Vol.10 No.5
<P>Magnetic properties of biological particles are measured in high-gradient magnetic separation (HGMS) analysis, revealing the concentrating process of nucleoprotein particles, ferritin, red blood cells, and eggs. A magnetic force acting on micrometer and submicrometer biological particles having diamagnetic or paramagnetic susceptibility with respect to the solution causes their movement and accumulation in gradient magnetic fields dependent on the values of the magnetic moments. The methods developed enable us to obtain the magnetic moments values of single particles and their assembly directly from magnetic separation and image analyses without assuming the detection of sizes. Our precision methods for the measurement of the capture traveling (magnetic diffusion) time and the accumulation (magnetic sedimentation) radius in HGMS show that it is really possible to determine the weak dia- or paramagnetic shifts of magnetic susceptibility up to 0.7x10(-10) (SI units). HGMS analysis of the concentrating process of nucleoprotein granules (microcells, DNA granules, or nucleosome core particles) with polarization microscopy reveals phase transitions for DNA in granules, and separation accumulation of particles enables the determination of the diamagnetic susceptibility and anisotropy properties. Magnetic concentration effects always occur in living systems because micrometer-located gradient magnetic fields inside an organism are strong enough to cause drifts of cellular complexes and organelles of micrometer and submicrometer sizes. We report the appearance of superparamagnetic contamination inside developing shrimp eggs. In the developing shrimps eggs, ferritin aggregates are observed under weak gradient magnetic fields and diaparaferromagnetic changes are detected. A significant interruption of egg development is revealed in such fields.</P>