The inhibitory function of Fc-ST2 depends on cell type; IL-1RAcP and ST2 are necessary but insufficient for IL-33 activity.

Hong, Kwangwon,Lee, Youngmin,Lee, Siyoung,Hong, Sungnoh,Bae, Suyoung,Hong, Jaewoo,Choi, Jida,Jhun, Hyunjhung,Kwak, Areum,Kim, Eunsom,Jo, Seunghyun,Kang, Taebong,Cho, You Sook,Kim, Yong-Gil,Kim, Soohyu S. Karger 2013 Immunologic research Vol.56 No.1

<P>IL-33 (IL-1F11) is a member of IL-1 family ligand, which stimulates the production of inflammatory cytokines. IL-33 receptor complex is comprised of IL-1 receptor accessory protein (IL-1RAcP) and ST2 that are activated by IL-33 ligand binding. ST2 is a ligand-binding chain of the IL-33 receptor component, and the soluble ST2 form possesses antagonistic activity. Here, we expressed the extracellular domain of ST2-fused to the immunoglobulin of IgG1 constant region in order to generate a soluble recombinant Fc-ST2. Human and mouse recombinant Fc-ST2 protein were expressed in Chinese hamster ovary cells and purified using a mini-protein A affinity chromatography. The recombinant Fc-ST2 protein was used to examine inhibitory function in IL-33-induced cytokine production in different cell types. The human Fc-ST2 abolished IL-33-induced IL-8 production in human mast cells, but mouse Fc-ST2 failed to inhibit IL-33-induced TNFα production in mouse Raw 264.7 macrophage cells. We further investigated the expression of IL-33 receptor component with various cell lines. IL-33 receptors expression pattern and Fc-ST2 inhibitory activity in different cell types suggest that IL-1RAcP and ST2 are necessary but insufficient for IL-33 activity. Our results suggest that an additional receptor component may participate in the biological activity of IL-33.</P>

Identification of Constitutively Active Interleukin 33 (IL-33) Splice Variant

Hong, Jaewoo,Bae, Suyoung,Jhun, Hyunjhung,Lee, Siyoung,Choi, Jida,Kang, Taebong,Kwak, Areum,Hong, Kwangwon,Kim, Eunsom,Jo, Seunghyun,Kim, Soohyun American Society for Biochemistry and Molecular Bi 2011 The Journal of biological chemistry Vol.286 No.22

<P>IL-33/IL-1F11 is a new member of the IL-1 family ligand and provokes T helper-type immune responses. IL-33 is the ligand of ST2 and IL-1 receptor accessory protein (IL-1RAcP) that triggers nuclear factor-κ light chain enhancer of activated B cells (NF-κB) and MAPK signaling. We discovered a novel short splice variant of <I>IL-33</I> that was termed <I>spIL-33</I>. The new <I>spIL-33</I> lacks exon 3 containing a proposed caspase-1 cleavage site. We isolated <I>spIL-33</I> cDNA from the Huh7 human hepatocarcinoma cell line and expressed the recombinant spIL-33 protein in Escherichia coli. The recombinant spIL-33 and pro-IL-33 were not cleaved by caspase-1, unlike IL-18 (IL-1F4). The recombinant spIL-33 was constitutively active, and spIL-33-induced inflammatory cytokine production was caspase-1-independent in HMC-1 and Raw 264.7 cells. The recombinant spIL-33 induced the phosphorylation of IL-1 receptor-associated kinase (IRAK1), NF-κB, p38 MAPK, p44/42 MAPK, and JNK in a time- and dose-dependent manner. Anti-ST2 monoclonal antibody specifically blocked the spIL-33-induced cytokine production. In this study, we identified and characterized a new IL-33 splice variant, which was a constitutively active IL-33 isoform. The existence of constitutively active spIL-33 suggests that the biological activity of IL-33 could be triggered by diverse stimulations during immune responses. Further investigation of the spIL-33 expression pattern may contribute to understanding the involvement of IL-33 in inflammatory disorders.</P>

Neutrophil Proteinase 3 Induces Diabetes in a Mouse Model of Glucose Tolerance

Bae, Suyoung,Choi, Jida,Hong, Jaewoo,Jhun, Hyunjhung,Hong, Kwangwon,Kang, Taebong,Song, Keeho,Jeong, Sangmin,Yum, Hokee,Kim, Soohyun Informa Healthcare 2012 Endocrine research Vol.37 No.1

<P>Type 1 diabetes is considered to be an autoimmune disease in which T cells attack pancreatic islet cells. Impaired glucose tolerance with type 2 diabetes has been classified as an obesity-associated metabolic syndrome. However, recent studies have revealed that type 2 diabetes is an autoinflammatory disease due to an imbalance of inflammatory cytokine production and related molecular components that cause inflammation. Insulin-like growth factor (IGF) and the insulin-like growth factor-binding protein-3 (IGFBP3) system are known to be involved in the development of experimental diabetic nephropathy, and urinary IGFBP3 protease activity has been observed in patients with type 2 diabetes. A serine protease was found to be responsible for the proteolytic activity in diabetic urine; however, the identity of the precise enzyme remains unknown. We investigated neutrophil proteinase 3 (PR3) to see whether it has specific enzymatic activity associated with insulin-like growth factor-1 and IGFBP3. In our study, both molecules were sufficiently degraded, which leads us to believe that PR3 may induce insulin resistance in the mouse model utilized. In addition, we found that PR3 in the urine of diabetic patients similarly affects insulin resistance. Moreover, PR3-immunized mice had an increase in glucose clearance due to inhibition of PR3 activity. As such, PR3 can be considered as an inflammatory enzyme directly linking inflammation to type 2 diabetes through downregulation of insulin-like growth factor-1/IGFBP3.</P>

Contradictory Functions (Activation/Termination) of Neutrophil Proteinase 3 Enzyme (PR3) in Interleukin-33 Biological Activity

Bae, Suyoung,Kang, Taebong,Hong, Jaewoo,Lee, Siyoung,Choi, Jida,Jhun, Hyunjhung,Kwak, Areum,Hong, Kwangwon,Kim, Eunsom,Jo, Seunghyun,Kim, Soohyun American Society for Biochemistry and Molecular Bi 2012 The Journal of biological chemistry Vol.287 No.11

Difficulty Scoring System of Pure Laparoscopic Donor Right Hemihepatectomy

Jeong-Moo Lee,Eunhye Shin,Suk Hee Ko,Kwangpyo Hong,Eui Soo Han,Sanggyun Suh,Suyoung Hong,Suk Kyun Hong,YoungRok Choi,Nam-Joon Yi,Kwang-Woong Lee,Kyung-Suk Suh 대한외과학회 2020 대한외과학회 학술대회 초록집 Vol.2020 No.11

A study on the localization at the U-turn section in urban driving scenario of the Autonomous vehicle

Suyoung Choi,Daehie Hong 한국자동차공학회 2019 한국자동차공학회 학술대회 및 전시회 Vol.2019 No.11

Hydrocotyle ramiflora attenuates benign prostatic hyperplasia in the rats

Suyoung Park,Eun Bok Baek,Eun-Ju Hong,Eunhye Jung,Warisraporn Tangchang,Hwa-Young Son,Hyo-Jung Kwon 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Benign prostatic hyperplasia (BPH) is a common urogenital disorder that affects up to 85% of males who are over 50 years-old. BPH is characterized by the increased proliferation of epithelial and stromal cells of the prostate. Hydrocotyle ramiflora (HR) of the family Apiaceae, is a perennial plant. HR is known as the induction of diuresis and antipyretic activity. And HR is used for therapy of disease of urinary system, such as ischuria and cystitis. The present study evaluated the therapeutic effects of HR on a testosterone-induced BPH rats. We induced BPH to rats by subcutaneous injections (s.c) of testosterone propionate (TP) daily for four weeks. Rats were also administered daily oral gavage of HR (150 mg/kg) or vehicle. After four weeks of induction, all animals were euthanized humanely and their prostate glands were removed, weighed and processed for further analysis, including histopathological examination, immunohistochemistry, real-time PCR, and western blot analysis. Administration of HR to TP-induced BPH rats considerably reduced prostate weight, epithelial thickness and expression of proliferating cell nuclear antigen (PCNA). The expression of cleavage of caspase-3 was markedly increased by HR-treatment in the rats. In addition, HR decreased the level of pro-inflammatory cytokines, including interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) and suppressed the expression of Vascular Endothelial Growth Factor(VEGF) and Transforming Growth Factor beta 1 (TGF-β1). The activation of nuclear factor-κB (NF-κB) and The expression of cyclooxygenase-2 (COX-2) were reduced by HR treatment in a TP-induced BPH rat model. These results indicate that HR is beneficial in the treatment of BPH by promoting the apoptosis and inhibiting the inflammation. Therefore, HR could be a useful agent in the treatment of BPH.

Generation of Anti-Proteinase 3 Monoclonal Antibodies and Development of Immunological Methods to Detect Endogenous Proteinase 3

Bae, Suyoung,Choi, Jida,Hong, Jaewoo,Lee, Siyoung,Her, Erk,Choi, Wonhyuk,Kim, Sangmin,Choi, Youngbum,Kim, Soohyun Mary Ann Liebert 2010 Hybridoma Vol.29 No.1

<P>Proteinase 3 (PR3), a neutrophil granule serine protease, is the major autoantigen for autoantibodies in the systemic vasculitic disease, Wegener's granulomatosis. It is also found to be involved in various inflammatory diseases including Crohn's disease, rheumatoid arthritis, cystic fibrosis, and gingivitis. However, there is no high quality antibody available to detect endogenous PR3 in biological samples such as plasma and tissue. Several commercial anti-PR3 monoclonal antibodies (MAbs) were obtained by using HMC-1/PR3 cell granule extracts as the antigen, but the resulting antibodies could not be applied for immunoblotting or other immunological methods. Therefore, we produced human recombinant PR3 in Escherichia coli and developed several MAbs that are highly sensitive and can be used for immunoblotting, FACS analysis, and immunofluorescent staining. The PR3 MAbs recognized both rhPR3 and human plasma-derived neutrophil PR3 in reducing and non-reducing conditions at low nanogram levels. In addition, new MAbs detect endogenous PR3 from normal human plasma and urine with high specificity. The new anti-PR3 MAbs will be an essential tool for investigating the role of PR3 in inflammatory and autoimmune diseases.</P>

성공적 노화와 무용에 관한 연구동향

최수영 ( Suyoung Choi ),홍예주 ( Yeju Hong ) 한국무용과학회 2021 한국무용과학회지 Vol.38 No.4

본 연구에서는 성공적인 노화와 무용과 관련된 연구의 연구동향을 분석하고 파악하여 차후 연구에 대한 시사점을 확인하고자 KCI 등재지 이상 2012년부터 2021년 7월 20일까지 발표된 연구물 총 120편을 수집하여 분석한 결과, 다음과 같은 결론을 얻었다. 첫째, 연구 연도별 동향에서 성공적인 노화와 무용과 관련된 연구는 2020, 2016, 2013년도에 가장 많이 발표되었고 2012년, 2015년을 제외한 모든 연도에서 10편 이상씩 꾸준히 발표된 것으로 나타났다. 게제학술지별 동향에서는 성공적인 노화와 무용과 관련된 연구 총 120편이 총 32개 학술지에 발표되었으며 한국무용과 학회지에 가장 많이 게제 되었고 대한무용학회논문집, 한국체육과학회지, 한국체육학회지, 한국무용연구, 한국콘텐츠학회논문지 등에 관련 연구물들이 게제된 것으로 나타났다. 차후 본 연구결과에서 나타난 바와 같이 관련 연구가 다양한 학술지에서도 발표되어 무용의 입지를 넓혀가면서 변화를 이끌어낼 필요성이 있다. 둘째, 연구 대상별 동향에서는 여성노인 대상의 연구가 가장 많았고 남성노인 대상의 연구는 가장 낮은 것으로 나타났지만 질병이 있는 노인 대상의 연구도 활발히 연구되고 있어 남성노인 대상의 연구 필요성과 함께 시대적 변화가 반영되는 현상을 확인할 수 있었다. 무용 유형별 동향에서는 한국무용이 가장 많이 연구되었고 현대무용은 가장 적게 연구되었으나 댄스스포츠, 라인댄스, 생활무용 등 한국무용외 다양한 무용들이 성공적인 노화관련 연구에 적극적으로 활용되고 있었다, 현재 관련 연구에서 생활무용, 건강무용, 실용무용, 사회무용 등 비슷한 개념을 가지고 있는 무용들이 제시되고 있어 차후 무용 유형에 대한 고민이 필요하다 여겨진다. 셋째, 연구 분야별 동향에서는 성공적 노화와 무용관련 연구가 자연과학분야와 인문사회 분야별로 균형감을 가지고 연구되고 있음을 확인할 수 있었다. 연구 방법별 동향에서는 양적연구가 질적연구보다 두드러지게 많이 연구되어 수치화에 의한 결과 제시로 연구목표를 달성하고자 하는 현상을 볼 수 있었다. 차후 개인차가 많은 노인을 이해하고 노화정보를 심층적으로 분석할 수 있는 질적연구가 지속적으로 요구된다. This study is to analyze the trend of research about successful aging and dance. Using the research information sharing service, a total of 120 papers published from 2012 to July 20, 2021 were analyzed. The research results are as follows. First, in terms of trends by study year, the papers on successful aging and dance were published the most in year 2020, 2016, and 2013. And more than 10 papers have been released every year consistently except in 2012 and 2015. Total 120 papers were published in 32 journals. The Journal of Korean Society of Dance Science has published the most research about successful aging and dance with 18 papers, and The Korean Journal of Dance, The Korea Journal of Sports Science, Korean journal of physical education, Journal of Korean Dance and The Contents association Review also have published related research. As shown in the results of this study, it is necessary to draw changes while expanding the base of dance by publishing related studies in various academic journals. Second, in terms of trends by study subject, the number of papers on the elderly female was the highest with 54 papers, and the number of papers on the elderly male was the lowest with 3 papers. On the other hand, there were 24 papers on the elderly with the disease, which shows the necessity of research on male elderly subjects and a phenomenon of the changing times reflected in studies. In terms of trends by dance type, Korean dance was studied the most with 28 papers and modern dance was studied the least with 2 papers. However, 16 papers about dance sports, 15 papers about line dance and 19 papers about life dance were studied, showing that various dances other than Korean dance were being studied actively in papers about successful aging. Recently, dances with similar concepts, such as life dance, health dance, practical dance and social dance are being suggested in recent related research, so it seems necessary to consider the type of dance of future. Third, In terms of trends by study field, successful aging and dance related studies were conducted at a similar rate between natural science field and humanities and social field, suggesting that related studies are being conducted in a balanced way. In terms of trends by study method, quantitative research was significantly conducted more than qualitative research, indicating the tendency to achieve the research goal by presenting the results by quantification. It suggests that to understand the elderly with many individual differences and to analyze aging information in depth, qualitative research is needed to be conducted more continuously.

사용자 선호도를 반영한 게임 하이라이트 자동 생성 시스템

배수영(Suyoung Bae),홍승진(Seung-Jin Hong),이상광(Sang-Kwang Lee) 한국통신학회 2023 한국통신학회 학술대회논문집 Vol.2023 No.6