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Sunhee Lee,Min Gon Kim,Hee Soo Lee,Sunhak Heo,Mirae Kwon,Geun- Bae Kim 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.2
In this study, two Listeria bacteriophages, LMP1 and LMP7, were isolated from chicken feces as a means of biocontrol of L. monocytogenes. Both bacteriophages had a lytic effect on L. monocytogenes ATCC 7644, 15313, 19114, and 19115. Phages LMP1 and LMP7 were able to inhibit the growth of L. monocytogenes ATCC 7644 and 19114 in tryptic soy broth at 10℃ and 30℃. Nevertheless, LMP1 was more effective than LMP7 at inhibiting L. monocytogenes ATCC 19114. On the contrary, LMP7 was more effective than LMP1 at inhibiting L. monocytogenes ATCC 7644. The morphology of LMP1 and LMP7 resembled that of members of the Siphoviridae family. The growth of L. monocytogenes ATCC 7644 was inhibited by both LMP1 and LMP7 in milk; however, the growth of L. monocytogenes ATCC 19114 was only inhibited by LMP1 at 30℃. The lytic activity of bacteriophages was also evaluated at 4℃ in milk in order to investigate the potential use of these phages in refrigerated products. In conclusion, these two bacteriophages exhibit different host specificities and characteristics, suggesting that they can be used as a component of a phage cocktail to control L. monocytogenes in the food industry.
Basic Design of the Cross-border Power Trading System for WAPP
Jinsu Lee(이진수),Hyosang Lee(이효상),Sunhak Kim(김성학) 대한전기학회 2009 대한전기학회 학술대회 논문집 Vol.2009 No.7
The West African Power Pool is currently developing the regional electricity market for its member states. As the basic design for the cross-border power trading system for the West African Power Pool, the step-by-step evolution, the Baseline System and the Fullscale System, is estimated. Along with the interconnection project between member countries, the various functions from the data acquisition and processing by the Baseline System to the interchange trading by the Fullscale System will be implemented gradually.
Inhibition of Clostridium perfringens using Bacteriophages and Bacteriocin Producing Strains
Sunhak Heo,Min Gon Kim,Mirae Kwon,Hee Soo Lee,Geun-Bae Kim 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.1
In this study, we isolated and characterized a bacteriocin-producing strain and two bacteriophages (P4, A3), showing antimicrobial effects against Clostridium perfringens, from chicken and swine feces by the spot-on-the lawn antagonism method. The selected strain was identified as Streptococcus hyointestinalis by 16S rRNA gene sequencing. The bacteriocin from the isolated strain exhibited strong inhibitory activity against four strains of C. perfringens and all the tested strains of Listeria monocytogenes, and the bacteriocin were highly heat- and pH-stable even at pH 2, pH 10 and 121°C for 15 min. We also evaluated the combined effects of the isolated bacteriocin and phages. Combining the phage treatments and bacteriocin resulted in a synergetic effect compared with the phage or the bacteriocin alone. In addition, during the probiotic test, the bacteriocin-producing S. hyointestinalis B19 strain reduced the population of C. perfringens significantly. Treatment with S. hyointestinalis B19 and a cocktail of lytic bacteriophages eradicated the C. perfringens KCTC 3269T, completely. Consequently, the isolated bacteriocin and bacteriophages represent candidates for effective biocontrol of C. perfringens, and bacteriocin-producing S. hyointestinalis B19 is a potential probiotic candidate for use in domestic animals.
( Ju-eun Lee ),( Sunhak Heo ),( Geun-bae Kim ) 한국축산학회(구 한국동물자원과학회) 2020 한국축산학회지 Vol.62 No.3
Streptococcus hyointestinalis B19 was isolated from chicken feces collected from local farm in Anseong, Korea. S. hyointestinalis B19 was shown to produce bacteriocin-like compounds exhibiting inhibitory activities against several pathogens including strains of Clostridium perfringens and Listeria monocytogenes. The whole genome of S. hyointestinalis B19 strain was sequenced using PacBio RS II platform. The genome comprised four contigs with a size of 2,217,061 bp. The DNA G + C content was found to be 42.95 mol%. Annotation results revealed 2,266 coding sequences (CDSs), 18 rRNAs, and 61 tRNA genes. Based on genome analysis, we found that the strain B19 possessed various genes associated with bacteriocin synthesis, modification, and transport.
Hee Soo Lee,Mirae Kwon,Sunhak Heo,Min Gon Kim,Geun-Bae Kim 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.4
This study investigated the psychrotrophic bacteria isolated from chicken meat to characterize their microbial composition during refrigerated storage. The bacterial community was identified by the Illumina MiSeq method based on bacterial DNA extracted from spoiled chicken meat. Molecular identification of the isolated psychrotrophic bacteria was carried out using 16S rDNA sequencing and their putrefactive potential was investigated by the growth at low temperature as well as their proteolytic activities in chicken meat. From the Illumina sequencing, a total of 187,671 reads were obtained from 12 chicken samples. Regardless of the type of chicken meat (i.e., whole meat and chicken breast) and storage temperatures (4°C and 10°C), Pseudomonas weihenstephanensis and Pseudomonas congelans were the most prominent bacterial species. Serratia spp. and Acinetobacter spp. were prominent in chicken breast and whole chicken meat, respectively. The 118 isolated strains of psychrotrophic bacteria comprised Pseudomonas spp. (58.48%), Serratia spp. (10.17%), and Morganella spp. (6.78%). All isolates grew well at 10°C and they induced different proteolytic activities depending on the species and strains. Parallel analysis of the next generation sequencing and culture dependent approach provides in-depth information on the biodiversity of the spoilage microbiota in chicken meat. Further study is needed to develop better preservation methods against these spoilage bacteria.
김유진,Hana Youn,Junbeom Kim,Daye Lee,Seonghye Go,Ji-eun Park,Sunhak Lee,Jinyong Noh,남상섭 대한백신학회 2020 Clinical and Experimental Vaccine Research Vol.9 No.2
Currently, no vaccine or established therapeutic agents are available for coronavirus disease 2019. The sharp increase in demand for personal protective equipment (PPE) necessitates an improvement in the protective efficacy of PPE. We evaluated the potential antimicrobial and antiviral effects of a surface-coating disinfectant (3-(trimethoxysilyl)propyldimethyl octadecyl ammonium chloride, Si-QAC) when applied onto PPE. Si-QAC-pre-coated PPE was artificially contaminated with either influenza virus or Salmonella. The results showed significantly reduced influenza and Salmonella titers in Si-QAC-coated PPE; these antimicrobial effects lasted 7 days. This suggests that this surface-coating disinfectant effectively reduces pathogen contamination of PPE, enabling their safe and long-term use.
LPGi 시스템의 SULEV Emission 개발 (Ⅱ)
강현홍(Hyunhong Kang),박병진(Byungjin Park),이동훈(Donghoon Lee),이선학(Sunhak Lee),조진행(Jinhaeng Cho),지선호(Sunho Ji) 한국자동차공학회 2010 한국자동차공학회 학술대회 및 전시회 Vol.2010 No.11
The trend of exhaust emission regulation requires SULEV which is much more stringent than ULEV. The LPGi (LPG gaseous injection) system vehicle has been commercialized, but is required to be improved to SULEV regulation. Acknowledged methods of Exhaust After Treatment system are EHC(Electrically Heated Catalyst), Spark Retard Control and SAI(Secondary Air Injection) which has been known as a method of reducing Catalyst light-off time. The focus of this paper is to reduce HC(Hydro-Carbon), CO(Carbon monoxide) & NOx(Nitrogen Oxides) emissions using fuel control with current LPGi system. In theory, the thermal efficiency and the vaporization performance of LPG fuel is better than gasoline fuel. Additionally, LPGi system does not have wall wetting phenomenon because the previously vaporized fuel is injected into cylinder directly. So in case of LPGi system, it is possible to control the exact fuel mass even from cold start. Thus LPGi system can reduce much of the exhaust HC even before catalytic converter will be activated. The purpose of this paper is to introduce the technology as to how to develop the engine control system to reduce the emissions for KSULEV and to share the KSULEV emission test result which was executed by the National Transportation Pollution Research Center.