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- 검색키워드
- 저자 : Sunghwan Shin (검색결과 70 건)
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Sunghwan Shin,유인영,Hyang Jin Shim,On-Kyun Kang,Byung Woo Jhun,Won-Jung Koh,허희재,Nam Yong Lee 대한진단검사의학회 2020 Annals of Laboratory Medicine Vol.40 No.2
The GENEDIA MTB/NTM Detection Kit (GENEDIA MTB/NTM; Green Cross Medical Science Corp., Chungbuk, Korea) is a multiplex real-time PCR assay used for differential identification of Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM). While the importance of differential identification of MTB/NTM is recognized, there is limited data on the performance of GENEDIA MTB/NTM assay to date. A total of 687 consecutive sputum specimens were cultured and analyzed with the GENEDIA MTB/NTM and GENEDIA MTB assays. Nineteen specimens (2.8%) were MTBC-positive, and 69 (10.0%) were NTM-positive based on mycobacterial culture. All specimens showed concordant results for MTBC using both assays, with a kappa value of 1.00, overall sensitivity of 63.2% (12/19), and specificity of 100% (668/668). The overall NTM sensitivity and specificity were 23.2% (16/69) and 99.7% (616/618) for GENEDIA MTB/NTM. The association between NTM-positivity using GENEDIA MTB/NTM and the diagnosis of NTM pulmonary disease was not statistically significant. In conclusion, the two real-time PCR assays showed similar diagnostic performance for MTBC detection. However, the sensitivity for NTM detection was lower than that for MTBC detection.
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Shin, Sunghwan,Kang, Hani,Kim, Jun Soo,Kang, Heon American Chemical Society 2014 The Journal of physical chemistry B Vol.118 No.47
<P>We investigated the phase transformations of amorphous solid acetone under confined geometry by preparing acetone films trapped in amorphous solid water (ASW) or CCl<SUB>4</SUB>. Reflection absorption infrared spectroscopy (RAIRS) and temperature-programmed desorption (TPD) were used to monitor the phase changes of the acetone sample with increasing temperature. An acetone film trapped in ASW shows an abrupt change in the RAIRS features of the acetone vibrational bands during heating from 80 to 100 K, which indicates the transformation of amorphous solid acetone to a molecularly aligned crystalline phase. Further heating of the sample to 140 K produces an isotropic solid phase, and eventually a fluid phase near 157 K, at which the acetone sample is probably trapped in a pressurized, superheated condition inside the ASW matrix. Inside a CCl<SUB>4</SUB> matrix, amorphous solid acetone crystallizes into a different, isotropic structure at ca. 90 K. We propose that the molecularly aligned crystalline phase formed in ASW is created by heterogeneous nucleation at the acetone–water interface, with resultant crystal growth, whereas the isotropic crystalline phase in CCl<SUB>4</SUB> is formed by homogeneous crystal growth starting from the bulk region of the acetone sample.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpcbfk/2014/jpcbfk.2014.118.issue-47/jp503997t/production/images/medium/jp-2014-03997t_0007.gif'></P>
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Shin Sunghwan,Oh Jongwon,Park Hyung-Doo 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.1
The Barricor tube (Becton Dickinson [BD], Sunnyvale, CA, USA) was recently developed to mechanically separate plasma by increasing the centrifugation rate. We compared the Barricor tube with existing serum- and plasma-based tubes based on 35 biochemical analytes and preanalytical turnaround time (TAT). Blood samples were collected from 30 healthy volunteers in a Barricor tube, serum separating tube (SST, Vacutainer SST II Tube 8.5 mL, #368972; BD), or plasma separating tube (PST, Vacutainer PST Tube 8.0 mL, #367964; BD) in random order. Next, 27 chemistry analytes, six immunochemistry analytes, and two cardiac markers were compared using Passing-Bablok regression and the Bland-Altman method. Preanalytical TAT was measured for each tube. The Barricor tube exhibited bias exceeding the desirable limit for nine and four analytes compared with the SST and PST, respectively. The Barricor tube lactate dehydrogenase value showed a bias of -10.29% and -9.86% compared with that of the SST and PST, respectively. The preanalytical TAT of Barricor tube was 8.8 minutes, which was the shortest among the three tubes. The clinical performance of the Barricor tube was equivalent to that of the SST and PST for most analytes, with an apparent advantage in preanalytical TAT. When using the Barricor tube, the reference range needs to be changed for some analytes that exceed the desirable bias limit.
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Shin Sunghwan,Oh Hyeonju,Park Hea Ree,Joo Eun Yeon,이수연 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.1
Melatonin and cortisol are clinically important for diagnosing sleep and mood disorders. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of salivary melatonin and cortisol concentrations according to the Clinical and Laboratory Standards Institute guidelines. Additionally, we compared the LC-MS/MS assay with immunoassays, ELISA (Direct Salivary Melatonin Elisa EK-DSM, Bühlmann Laboratories AG, Schönenbuch, Switzerland) and electrochemiluminescence immunoassay (Cortisol II, Roche, Mannheim, Germany), using 121 saliva samples. The LC-MS/MS assay exhibited good performance in terms of linearity, precision, accuracy, limit of detection, lower limit of quantification, extraction recovery, carry-over, and matrix effect. The LC-MS/MS assay and immunoassays showed strong correlation (Pearson’s r=0.910 for melatonin, r=0.955 for cortisol), but demonstrated a significant mean bias of 23.2% (range 54.0–143.7%) for melatonin and 48.9% (range 59.7–184.7%) for cortisol. Our LC-MS/MS assay provided more sensitive and reliable salivary melatonin and cortisol quantification results compared with immunoassays.
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Psychological effects and risk perception after genetic counseling
Shin, Sunghwan,Ryu, Mi Ra,Kwon, Won Kyung,Kim, Suhee,Jang, Ja-Hyun,Kim, Jong-Won Korean Society of Medical Genetics and Genomics 2021 대한의학유전학회지 Vol.18 No.1
Purpose: Demand for genetic counseling on cancer predisposition syndrome is increasing. We evaluated the psychological effect on counselees after genetic counseling at a clinic in a single center. Materials and Methods: We surveyed a total of 72 enrolled participants who visited a genetic counseling clinic at the Samsung Medical Center (SMC). The initial survey was conducted before the first genetic counseling session, and the second survey was conducted after the second genetic counseling session. A total of 43 participants completed both the initial and second surveys. Results: The initial survey of 72 participants indicated higher feelings of guilt in the group with religion, higher depression and anxiety in the group with a diagnosis of self, and higher anxiety in the group on self-referral to the genetic counseling clinic. In the completed survey of 43 participants, overall decreased depression was observed after the second genetic counseling session (P=0.013). Risk perception and anxiety decreased in the group diagnosed with benign variant/variant of uncertain significance (BV/VUS, 25/3) and increased in the group diagnosed with pathogenic variant (PV, 15). Risk perception and anxiety differed between the BV/VUS and PV groups (P<0.001 and P=0.03, respectively). Conclusion: The genetic counseling clinic at the SMC was effective in ameliorating the depression score. Assessment of survey results revealed different depression scores, feelings of guilt and anxiety, and different effects of the genetic counseling clinic, depending on the subgroups. Understanding the needs and psychological characteristics of different groups is necessary for improving genetic counseling services.
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Shin, Sunghwan,Park, Youngwook,Kang, Hani,Kang, Heon American Chemical Society 2018 The Journal of Physical Chemistry Part C Vol.122 No.9
<P>In this work, we studied the effect of an applied electric field on the dissociation of acetic acid (AA) at the interface of the acid and ammonia molecular layers using the ice film capacitor method. The dissociation of AA was monitored by reflection absorption infrared spectroscopy, which measured the changes in the intensities of the vibrational modes of AA and the acetate anion. The extent of field-induced dissociation was found to be dependent on the direction of the applied electric field. Interestingly, the extent of acid dissociation was greater when the field was applied in the opposite direction to that of the proton-transfer motion. This suggested that the reaction was aided by the reorientation of the reagent molecules rather than by electrostatic stabilization of the charge-transfer energy. The effect of molecular size was studied by comparing the results for AA with those of formic acid and propionic acid. The dissociation of smaller acids was more strongly enhanced than that of larger acids, supporting the interpretation that the field-induced dissociation occurred via molecular reorientation.</P> [FIG OMISSION]</BR>
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Shin, Sunghwan,Park, Youngwook,Kim, Youngsoon,Kang, Heon American Chemical Society 2017 The Journal of Physical Chemistry Part C Vol.121 No.23
<P>We studied the ionic dissociation of trifluoroacetic acid (TFA) in D2O amorphous solid water (ASW) using reflection absorption infrared spectroscopy, low-energy sputtering, and H/D isotopic exchange experiment. TFA readily dissociated to hydronium and counterions in ASW at 60 K, which indicates a significant increase in the acidity as compared with that in aqueous solution at room temperature. The acid dissociation produced a Zundel continuum band in the 1000-3000 cm(-1) region and an accompanying lowered intensity of the O-D stretching band of D2O. The reduced intensity of D2O was several times larger than that expected for 1:1 stoichiometric proton transfer from TFA to water. Excess protons released from the acid migrated through as many as 20 water layers in ASW. These observations indicate that excess protons are highly mobile and dynamically delocalized in the hydrogen-bonded water chain. Such Characteristics of excess protons may be responsible for the appearance of the Zundel continuum absorption.</P>
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Shin, Sunghwan,Kim, Youngsoon,Kang, Hani,Kang, Heon American Chemical Society 2015 The Journal of Physical Chemistry Part C Vol.119 No.27
<P>We investigated the dipolar reorientation of acetone molecules in amorphous solids under the influence of externally applied electric fields in the range of (0–4.3) × 10<SUP>8</SUP> V·m<SUP>–1</SUP>. The electric field was applied using an ice film capacitor method, and the field strength was estimated from measurements of the film voltage and thickness using a Kelvin probe and the temperature-programed desorption method, respectively. Reorientation of the acetone molecules was monitored with reflection–absorption infrared spectroscopy (RAIRS), which measured the absorbance change of the acetone vibrational bands induced by the applied electric field. The electric field caused a substantial degree of dipolar polarization of the sample. Acetone molecules were reoriented toward the field direction by an average angle of about 31° at an applied field strength of 4.3 × 10<SUP>8</SUP> V·m<SUP>–1</SUP>, according to analysis of the RAIRS intensity changes using a simple molecular geometry model. While the extent of dipolar polarization of the sample increased with increasing field strength, the sample was not reversibly depolarized with decreasing field strength. Multiple-peak analysis of the ν(CO) spectrum revealed that the molecules in the acetone–water boundary region were more easily rotated compared to those in the bulk.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2015/jpccck.2015.119.issue-27/acs.jpcc.5b01849/production/images/medium/jp-2015-01849u_0009.gif'></P>
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Shin, Sunghwan,Park, Youngwook,Kang, Hani,Kang, Heon American Chemical Society 2017 The Journal of Physical Chemistry Part C Vol.121 No.45
<P>In this work, we studied the effect of an externally applied electric field on the conformation and orientation of 1,2-dichloroethane (DCE) in a frozen molecular solid. An electric field in the order of 10<SUP>8</SUP> V m<SUP>–1</SUP> was applied across the sample by means of interfacial charging of Cs<SUP>+</SUP> ions. Reflection absorption infrared spectroscopy (RAIRS) was used to monitor the <I>gauche</I>/<I>trans</I> conversion and reorientation of the DCE molecules due to an electrostatic interaction with the field. <I>trans</I>-DCE molecules were converted to <I>gauche</I> conformers via internal C–C rotation with the conversion efficiency of 24% at the field strength of 1.5 × 10<SUP>8</SUP> V m<SUP>–1</SUP> and temperature 75 K. <I>gauche</I>-DCE molecules were reoriented along the field at an average tilt angle of 36° under the same conditions. These field-induced molecular changes were prohibited at 8 K and visible above 50 K. Therefore, both electrostatic and thermal energies are required to induce molecular transformations in the metastable solid by overcoming the activation barrier. This study suggests the possibility of controlling molecular conformation and orientation of condensed molecular solids by applying an external electric field whose magnitude is comparable to that of the intermolecular electric fields.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2017/jpccck.2017.121.issue-45/acs.jpcc.7b09064/production/images/medium/jp-2017-090647_0006.gif'></P>
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