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Oh, Sumi,Lee, Chankyu,Kim, SungHwa,Choi, HyungSeok,Kim, Mi-Ja,Lee, JaeHwan Wiley (John WileySons) 2017 Journal of the American Oil Chemists' Society Vol.94 No.11
<P>Effects of deuteron (D+) and electron donors on the oxidative stability in linoleic acid-water model systems were evaluated by analyzing headspace oxygen content and headspace volatiles. Acetic acid-d and tetrakis(dimethylamino)ethylene (TDAE) were selected as a deuteron and an electron donor, respectively. Samples containing acetic acid-d had significantly lower headspace oxygen content than controls while those containing TDAE had significantly higher headspace oxygen content (p < 0.05). Combination of acetic acid-d and TDAE accelerated the consumption of headspace oxygen. Volatiles including t-2-heptenal, 2-octenal, or 2,4-octadienal had higher mass to charge ratio (m/z) of (molecular weight +1)/molecular weight in samples with deuterium oxide than in samples with deuterium free water. However, no significant difference was observed in the m/z ratio of (molecular weight +1)/molecular weight of those volatiles among samples with or without deuteron or electron donors. Also, lipid hydroperoxides with deuterium, were not found in samples containing deuterium oxide and acetic acid-d. Therefore, added acetic acid-d may not be involved on the formation of lipid hydroperoxides and volatiles directly.</P>
Whitening Effects of GC/MS Components from Scolopendra subspinipes mutilans Fractions
JiEun LEE,SungHwa OH,YuSeon NOH,JiHun SEOK,YongJoo KIM,YoungSu JANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
This study was performed to determine the whitening effect of several solvent fractions of Scolopedra subspinipes mutilans(SSM) extracts. Fractions from ethanol and distilled water extracts of SSM were prepared by a systematic fractionation procedure using n-hexane, chloroform, ethyl acetate, n-butanol and d.w. Also, Cholroform, ethyl acetate and n-hexane extractions of SSM were analyzed by gas chromatography-mass spectrometry (GC-MS). The results demonstrated that 4 components were identified totally in samples, and 4-Hydroxyphenyl acetic acid(4-HPAA) was confirmed as a selective component for whitening effect and melanin synthesis in B16F10 melanoma cells. The 4-HPAA showed the tyrosinase inhibition activity to 77.34% and concentration-dependent. These results showed that fractions from SSM and 4-HPAA could be developed as a skin whitening material in cosmetics.
JiEun LEE,SungHwa OH,YuSeon NOH,JiHun SEOK,YongJoo KIM,YoungSu JANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Microcapsules could be used in cosmetics, pharmaceutical, quasi drugs and medical applications. Poly(ϵ-caprolactone) (PCL), a biodegradable and biocompatible polymer is useful to encapsulate a wide range of drugs making it material for the preparation of carriers with potential applications in therapeutics. Poly(ϵ-caprolactone) microcapusules containing Scolopedera subspinipes Extract were prepared by solvent evaporation method. Poly(vinyl alcohol) was used as a emulsifier and dichloromethane was used as a solvent for PCL and Scolopedera subspinipes Extract. Image analyzer and scanning electron microscope (SEM) were used to observe the shape and surface of microcapsule. Thermal behaviors were studied by using a differential scanning calorimetry (DSC). The partical size of microcapsules were measured by PSA(particle size analyzer). Specific peaks of Scolopedera subspinipes Extract in microcapsules were confirmed using FT-IR spectra.
Kim Kyung Eun,Nguyen Nhan Thi,Kim Sun Ho,Bahk Sunghwa,Cheong Mi Sun,Park Hyeong Cheol,Lee Kyun Oh,Hong Jong Chan,Chung Woo Sik 한국식물학회 2022 Journal of Plant Biology Vol.65 No.1
Mitogen-activated protein kinases (MPKs) play roles as critical signal components in the environmental stress responses and developmental processes in plants. Calcium ion (Ca2+) is one of the most essential ubiquitous intracellular second messengers involved in many signal transduction pathways in plants. It was previously known that MPKs are activated by the increasing Ca2+ concentration. However, the mechanism of how Ca2+ activates MPKs is not elucidated yet. In this study, we revealed that Ca2+ could activate MPK signaling pathway via inhibiting the activity of a dual-specificity protein phosphatase1 (DsPTP1) by Ca2+/calmodulin (CaM). We showed that DsPTP1 directly interacts with MPK6 in vitro and in vivo. DsPTP1 was able to inactivate the active MPK6 by dephosphorylation. Interestingly, the DsPTP1-mediated dephosphorylation of MPK6 was strongly inhibited by Ca2+/CaM. Moreover, this inhibition was caused by the binding of CaM to the calmodulin-binding domain II (CaMBDII) of DsPTP1. This study implies that Ca2+/CaM is involved in the activation of MPKs through the inhibition of DsPTP1.