충수염의 임상적 고찰과 초음파의 유용성

박정배,양혁준,김형수,황성연,박철완,이근 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Appendicitis is the most common acute abdomen in Emergency Medical Center & outpatient department. But sometimes, it is difficult for us to diagnose because of clinically variable symptoms. We reviewed the ultrasonographic findings and analyse 521 cases of appendectomy patients about its distribution of sex and age, seasonal prevalence, clinical symptom and signs, laboratory findings, duration from onset of symptom to arrival at hospital, duration from arrival at hospital to operation, vital signs, operative results, and associated diseases, which were treated at the department of General Surgery & Emergency Medical Center in Chung Ang Gil Hospital from July, 1994 to June, 1995. The results were as follows : 1. The most common age group was second decade. And male to female ratio was 1.43:1. Seasonal variation was not significant. 2. Of 521 cases, 141 cases(27.1%) visited the hospital within 24 hours from the onset of symptom, and 259 cases(49.7%) visited between 24 to 48 hours. 3. In duration from arrival to operation, the cases within 6 hours were 263 (50.5%), the cases between 6 and 12 hours were 131(25..1%). 4. In systolic blood pressure, cases below 90mmHg were 19(3.6%), cases above 160mmHg were 23(4.4%). Below 60mmHg diastolic pressure were 55 cases(10.6%), cases above 110mmHg were 9(1.7%). In pulse rate, above 90 rate/min were 171(32.8%). 5. On admission leukocytosis was found in 413 cases(79.3%) and 342 cases of them were pathologic appendicitis. 6. Ultrasoundgraphy was performed in the 87 cases, but 23 cases were suggested appendicitis. Among 23 cases, 22(95.7%) revealed pathologic appendicitis at ooperation and labortatory examination. Of the 64 cases which were normal on ultrasonography, only 2 cases(3.1%) were proved to be appendicitis. 7. The results of 496 operations without ultrasonography were appendicitis in 390 cases(78.6%), mesenteric lymphadenopathy 36(7.3%), pelvic inflammatory disease 28(5.6%), fecalith 15(3.0%), terminal ileitis 8(1.6%), diverticulitis 7(1.4%), urinary tract stones 5(1.0%), cecal cancer 2 cases, right ovarian tumor 2 cases, tubo-ovarian abscess 2 cases, grater omentum internal hernia 1 case. Our results showed that the sensitivity of ultrasonohraphy was 95.8% and the specificity 98.4% in the diagnosis of appendicitis, It suggests that the ultrasonography is very helpful in the diagnosis & differential diagnosis of acute appeendiditis.

광센서를 위한 ZnO:Al 투명도전막의 제조 및 특성연구

박기철,이형기,심호섭 경북대학교 센서기술연구소 1995 연차보고서 Vol.1995 No.-

RF 마그네트론 스퍼터링법으로 AZO(ZnO:Al)막을 증착시켰다. 타겟내의 Al_2O_3의 첨가량, 기판온도, 분위기압, RF출력 등의 증착조건의 변화에 따라 제조된 AZO막의 조성, Al 도핑양, 구조적 특성 등을 XRD, SEM, EDS 및 RBS로 조사하였다. 이와 함께 전기적 및 광학적 특성을 Hall 효과 및 광투과도 특성을 통하여 조사하였다. 증착된 AZO막은 육방정의 wurtzite 구조였으며 모든 증착조건에서 기판에 수직으로 성장하는 (002)면 방향을 가지는 c-축배향성을 나타내었다. 증착된 AZO막내의 AB 도핑량은 타겟내의 무게비에 비례하였으며 Zn : O의 조성비토 1 : 1로 거의 일정하였다. 투명도전막으로서의 최적조건은 3wt%의 Al_2O_3이 첨가된 타겟으로 기판온도가 150℃, 분위기압이 2mTorr, RF출력이 150W로 증착한 경우였으며 이 때 비저항은 4.7×10 exp (4)Ω㎝, 캐리어 농도는 7.5×10 exp (20)㎝^-3이었다. 또 두께가 1500Å인 막의 경우 550㎚에서 광투고도가 90%이상이었으며 광학적 밴드갭은 3.53eV였다. Aluminium doped zinc oxide(AZO)films were prepared by RF magnetron sputtering method. The chemical compositions, doping concentrations, and the structural char-acteristics of the AzO films were studied in accordance with various deposition parameters, such as Al_2O_3 amount, substrate temperature and working pressure, by XRD, EDS, SEM and RBS. And The electrical and optical properties of AZO films were characterized by Hall effect and optical transmittance measurements. AZO films had were hexagonal wurtzite structure and dominent c-axis orientation. The Al doping concentration in the films were monotonically increased. The atomic ration of Zn : O were 1 : 1. The substrate, working pressure and rf power for optimum condition to fabricate the transparet conductive films using a target containing 3wt% Al_2O_3 were 150 ℃, 2mTorr and 150W, respectively. The resistivity and the carrier density of the AZO film prepared under this condition were 4.7 × 10 exp (-4) Ω㎝ and 7.5 × 10 exp (20) ㎝^-3, respectively. The optical transmittance of 1500 Å thick films at 550㎚ is ∼90% and optical energy band gap was 3.53eV.

배추 Plug育苗條件이 苗素質에 미치는 影響

李喆浩,崔周鎬,朴重春,孫嶺杰,朴鳳植,李炯貞 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

배추 plug육묘 조건을 알아보기 위하여 상토 종류, 질소시비수준, plug트레이 배수공수준을 달리하여 실험한 결과를 요약하면 다음과 같다. 1. 유묘출현율은 A상토<B상토<C상토의 順으로 높았고, 질소시비수준은 무비구에서 높고, 시비구에서는 90∼90ppm수준에서 높은 편이었으며 배수공수에 따라서는 배수공이 적은 128공구가 출현율이 높았다. 2. 幼苗의 葉발생도 A상토<B상토<C상토 순으로 높았고, 질소시비수준은 다비구(150ppm구)에서 엽수 5배로 최대엽수를 나타내었고, 배수공수에 따라서는 배수공이 적은 128공구가 높았다. 3. 엽장의 길이는 A상토구는 128공구에서 질소시비수준 120ppm에서 6.98cm로 가장 길었으며, B상토구에서 128배수공구의 시비수준 150ppm에서 12.41cm로 가장 길었고, C상토구에서도 128배수공구의 시비수준 150ppm에서 12.88cm, 200배수공구에서도 9.29cm로 가장 길었고 무비구에서는 공히 가장 짧았다. 4. 葉幅도 엽수와 엽장과 비슷한 경향으로 기비로 사용된 질소의 시비수준이 증가할수록 엽장이나 엽폭이 증가하여 B상토구의 150ppm 시비구에서 4.80cm로 최대치를 나타냈으며 시비량이 감소할수록 엽폭도 줄어드는 경향이였다. 5. 뿌리의 신장은 A상토구의 128배수공구에서 질소질 함량이 90∼120ppm수준의 범위내에서 신장이 양호하였으나 150ppm시비구에서는 불량한 반면, B와 C상토구에서는 90∼150ppm 모두에서 양호하였다. 6. 생체중과 건물중도 상토조성, 질소질비료 및 트레이 크기에 따라 모두 크게 영향을 받는 것으로 나타났으며 C상토구의 150ppm시비구에서 주당 3.0g의 생체중에서 최대치를 보였고 건물중 역시 C상토구의 150ppm시비구에서 4주에 600mg의 생체중의 증가를 보여 최대의 기록을 나타냈고 질소질의 농도 150ppm이 생체중 및 건물중 증가에 가장 효과적인 것으로 나타났다. The experiments were conducted to develop ideal substrates for the production of good quality in cabbage plug and also to improve the media with low or non environmental pollution by blending and mixing artificial substrate including peat moss, coir, perlite, vermiculite, carbonized rice hull and zeolite. In effects of soil substrate combination, nitrogen concentration and tray cell size on growing of 'Baekro', chinese cabbage, the ratio of seedling emergency in all fertilizer block was higher than non-fertilizer of nitrogen by 80%. The number of leaves were most increased in the 150ppm nitrogen solution of B block(coir 50%, vermiculite 10% and perlite+carbonized rice hull-zeolite 40%) and C block(coir 40%, vermiculite 20% and perlite+carbonized rice hull+zeolite 40%). The leaf length and leaf width were similar with that result in the number of leaves. Rooting condition was the best in the 90∼120ppm nitrogen solution of A block(TOSILI No1, SHINAN-JEONGMIL(Co)) in 128 tray cell size. The fresh weight and dry weight were increased as the order A<B<C in soil combination, were increased in the fertilizer which has more nitrogen concentration and were increased higher in 128 than 200 tray cell size, and chlorophyll concentration was the highest in the 150ppm nitrogen solution of A and B soil combination.

Pathogen- and NaC1-Induced Expression of the SCaM-4 Promoter Is Mediated in Part by a GT-1 Box That Interacts with a GT-1-Like Transcription Factor

Park, Hyeong-Cheol,Kim, Man-Lyang,Kang, Yun-Hwan,Jeon, Joo-Mi,Yoo, Jae-Hyuk,Kim, Min-Chul,Park, Chan-Young,Jeong, Jae-Cheol,Moon, Byeong-Cheol,Lee, Ju-Huck,Yoon, Hae-Won,Lee, Sung-Ho,Chung, Woo-Sik,Li Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

The Ca^(2+)-binding protein calmodulin mediates cellular Ca^(2+) signals in response to a wide array of stimuli in higher eukaryotes. Plants express numerous CaM isoforms. Transcription of one soybean (Glycine max) CaM isoform, SCaM-4, is dramatically induced within 30 min of pathogen or NaCl stresses. To characterize the cis-acting element(s) of this gene, we isolated an approximately 2-kb promoter sequence of the gene. Deletion analysis of the promoter revealed that a 130-bp region located between nucleotide positions -858 and -728 is required for the stressors to induce expression of SCaM-4. A hexameric DNA sequence within this region, GAAAAA (GT-1 cis-element), was identified as a core cis-acting element for the induction of the SCaM-4 gene. The GT-1 cis-element interacts with an Arabidopsis GT-1-like transcription factor, AtGT-3b, in vitro and in a yeast selection system. Transcription of AtGT-3b is also rapidly induced within 30 min after pathogen and NaCl treatment. These results suggest that an interaction between? GT-1 cis-element and a GT-1-like transcription factor plays a role in pathogen- and salt-induced SCaM-4 gene expression in both soybean and Arabidopsis.

Functional analysis of the stress-inducible soybean calmodulin isoform-4 (GmCaM-4) promoter in transgenic tobacco plants

Park, Hyeong Cheol,Kim, Man Lyang,Kang, Yun Hwan,Jeong, Jae Cheol,Cheong, Mi Sun,Choi, Wonkyun,Lee, Sang Yeol,Cho, Moo Je,Kim, Min Chul,Chung, Woo Sik,Yun, Dae-Jin Springer-Verlag 2009 Molecules and cells Vol.27 No.4

<P>The transcription of soybean (Glycine max) calmodulin isoform-4 (GmCaM-4) is dramatically induced within 0.5 h of exposure to pathogen or NaCl. Core cis-acting elements that regulate the expression of the GmCaM-4 gene in response to pathogen and salt stress were previously identified, between -1,207 and -1,128 bp, and between -858 and -728 bp, in the GmCaM-4 promoter. Here, we characterized the properties of the DNA-binding complexes that form at the two core cis-acting elements of the GmCaM-4 promoter in pathogen-treated nuclear extracts. We generated GUS reporter constructs harboring various deletions of approximately 1.3-kb GmCaM-4 promoter, and analyzed GUS expression in tobacco plants transformed with these constructs. The GUS expression analysis suggested that the two previously identified core regions are involved in inducing GmCaM-4 expression in the heterologous system. Finally, a transient expression assay of Arabidopsis protoplasts showed that the GmCaM-4 promoter produced greater levels of GUS activity than did the CaMV35S promoter after pathogen or NaCl treatments, suggesting that the GmCaM-4 promoter may be useful in the production of conditional gene expression systems.</P>

Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice

PARK, HYEONG CHEOL,KIM, HUN,KOO, SUNG CHEOL,PARK, HEE JIN,CHEONG, MI SUN,HONG, HYEWON,BAEK, DONGWON,CHUNG, WOO SIK,KIM, DOH HOON,BRESSAN, RAY A.,LEE, SANG YEOL,BOHNERT, HANS J.,YUN, DAE-JIN Blackwell Publishing Ltd 2010 Plant, cell and environment Vol.33 No.11

<P>ABSTRACT</P><P>Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of <I>Oryza sativa</I> SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of <I>Arabidopsis</I> SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of <I>OsSIZ1</I> and <I>OsSIZ2</I> in <I>siz1-2 Arabidopsis</I> plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of <I>siz1-2</I> seed germination was partially suppressed by <I>OsSIZ1</I> and <I>OsSIZ2.</I> The results suggest that rice SIZ1 and SIZ2 are able to functionally complement <I>Arabidopsis</I> SIZ1 in the SUMO conjugation pathway. Their effects on the <I>Arabidopsis</I> mutant suggest a function for these genes related to stress responses and stress adaptation.</P>

Isolation and Characterization of a Calmodulin-binding Ca²⁺-ATPase 2 (SCA2) in Soybean

Hyeong Cheol Park(박형철),Ho Soo Kim(김호수),Sang Min Lee(이상민),Hyeon Seol Cho(조현설),Woo Sik Chung(정우식) 한국생명과학회 2011 생명과학회지 Vol.21 No.5

대두의 세포막에 존재하는 SCA1은 칼모듈린에 의해서 조절된다는 내용을 이전에 보고하였다. 본 연구에서는 대두의 Ca²?-ATPase인 SCA2에 관한 특성을 연구하였다. SCA2는 SCA1과 아미노산 서열 비교에서 78%로 높은 유사성을 나타내며, 10개의 transmembrane 도메인이 존재하는 것을 확인하였다. CaM overaly assay로부터, SCA2는 칼슘에 의존적인 방법으로 칼모듈린과 결합한다는 것을 보여주었으며, Southern blot 분석 결과, 대두의 genome에는 두 종류의 Ca²?-ATPase가 존재하는 것으로 보인다. SCA2의 Ca²?-ATPase 효소활성을 확인하고자 yeast mutant를 이용하여 complementation assay를 수행해 보면, SCA2가 Ca²?-ATPase의 효소활성을 가지는 것을 보여 주었다. 이러한 결과들은 SCA2가 식물에 존재하는 type IIB Ca²?-ATPase들과 구조적으로 높은 유사성을 가진다는 것을 시사한다. We previously reported the isolation and characterization of a gene, SCA1 (for soybean Ca²?-ATPase 1), encoding a calmodulin-regulated Ca²?-ATPase that is located in the plasma membrane in soybean. Here, a Ca²?-ATPase designated as SCA2 was isolated from soybean. The two Ca²?-ATPases, SCA1 and SCA2, share a remarkably high degree of similarity (78%). Ten transmemebrane domains were predicted by hydropathy analysis. Using gel overlay assays, CaM was found to bind to SCA2 in a Ca²?-dependent manner. Southern blot analysis revealed the presence of two copies of the Ca²?-ATPase gene in the soybean genome. An N-terminal truncation mutant that deletes sequence through the putative calmodulin binding site was able to complement a yeast mutant (K616) that was deficient in two endogenous Ca²? pumps. Our results indicate that SCA2 is structurally highly conserved with type IIB Ca²? pumps in plants.

Stamen-Specific Expression of a Plant Defensin-Type Gene in Chinese Cabbage

Park, Hyeong-Cheol,Cheong, Yong-Hwa,Kim, Min-Chul,Kim, Cha-Young,Koo, Ja-Choon,Chun, Hyun-Jin,Lim, Chae-Oh,Cho, Moo-Je Gyeongsang National University Chinju, Korea 1998 Plant molecular biology and biotechnology research Vol.1998 No.

Pathogenesis-related Gene Expression by Specific Calmodulin Isoforms Is Dependent on NIM1, a Key Regulator of Systemic Acquired Resistance

Park, Chan-Young,Heo, Won-Do,Yoo, Jae-Hyuk,Lee, Ju-Huck,Kim, Min-Chul,Chun, Hyun-Jin,Moon, Byeong-Cheol,Kim, Ihn-Hyeong,Park, Hyeong-Cheol,Choi, Man-Soo,Ok, Hyun-Mi,Cheong, Mi-Sun,Lee, Sang-Min,Kim, H Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

Plants produce numerous calmodulin isoforms that exhibit differential gene expression patterns and sense different Ca^(2+)signals. This diversity results in different physiological responses to particular stimuli. GmCaM-4 and -5 are two divergent calmodulin isoforms from the soybean (Glycine max) that have been reported to be involved in plant disease resistance. However, little is known about the pathway by which these specific isoforms transduce the defense signal and up-regulate pathogenesis-related (PR) genes. Here we report that overexpression of GmCaM-4/-5 induces constitutive PR gene expression and enhances disease resistance in wild-type Arabidopsis, but not in the nim1 mutant of Arabidopsis. GmCaM-4/-5 also appear to activate trans-acting elements that bind to cis-acting elements in the Arabidopsis PR-1 promoter. Thus up-regulation of PR genes by these GmCaM isoforms is dependent on NIM1 (Non immunity 1) and unknown transcription factors.

Solvent-tolerance and trehalose accumulation by expression of otsA and otsB homologs in the response to toluene of Pseudomonas savastanoi BCNU 106 isolated from waste water

Hyeong Cheol Park,Yun Ui Bae,Ki Wook Kim,Soo Dong Cho,Ja Young Moon,Yong Kee Jeong,Woo Hong Joo 한국생명과학회 2003 한국생명과학회 심포지움 Vol.39 No.-