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Peripheral Blood NK Cells Reflect Changes in Decidual NK Cells in Women With Recurrent Miscarriages
Park, Dong Wook,Lee, Hyun Joo,Park, Chan Woo,Hong, Sung Ran,Kwak-Kim, Joanne,Yang, Kwang Moon Blackwell Publishing Ltd 2010 American journal of reproductive immunology Vol.63 No.2
<P><B>Citation</B> Park DW, Lee HJ, Park CW, Hong SR, Kwak-Kim J, Yang KM. Peripheral blood NK cells reflect changes in decidual NK cells in women with recurrent miscarriages. Am J Reprod Immunol 2010; 63: 173–180</P><P>Problem </P><P>We aimed to investigate if peripheral blood natural killer (pNK) cell levels are correlated with decidual NK (dNK) cell levels, and if chemokine expression has any role in dNK cell regulation.</P><P>Method of study </P><P>Decidual tissues of women having two or more miscarriages with normal karyotype were collected after miscarriage and an immuno-histochemisty study was made. pNK cells were evaluated using flow cytometric analysis.</P><P>Results </P><P>The %CD3<SUP>−</SUP>/56<SUP>+</SUP> and %CD3<SUP>−</SUP>/56<SUP>+</SUP>/16<SUP>+</SUP> pNK cells showed a significant correlation with mean number of CD56<SUP>+</SUP> dNK cells. The number of decidual CD16<SUP>+</SUP> cells was significantly higher in women with elevated pNK (≥15%) than that of normal pNK (<15%). The %CD3<SUP>−</SUP>/56<SUP>+</SUP> and %CD3<SUP>−</SUP>/56<SUP>+</SUP>/16<SUP>+</SUP> pNK cells showed an inverse correlation with duration of gestation. The CCL3<SUP>+</SUP> and CXCL12<SUP>+</SUP> cells were present in the decidua; however, staining intensity was not correlated with number of dNK cells.</P><P>Conclusion </P><P>The pNK cell levels reflect changes in dNK cell levels. This implicates that pNK cell level is a clinically useful marker to predict pregnancy outcome. Further study is needed to examine if elevated pNK cells enhance recruitment of dNK cells in the decidua.</P>
Kwak, Tae Joon,Kwon, Wookkun,Yang, Jiang,Lee, Sang Woo,Chang, Woo-Jin The Royal Society of Chemistry 2018 Analytical methods Vol.10 No.38
<P>Paper fluidics has recently offered an approach to precisely guide liquid flow in analytical devices with a low-cost regime. Multiple paper layers expand the capability of analytical devices to handle multiple samples as well as multiple detections simultaneously. Here we present a novel inexpensive cut-and-insert method to achieve a well-controlled even distribution of liquid in multi-channel three-dimensional (3D) paper-based analytical devices. A novel vacuum-driven poly dimethyl siloxane (PDMS) stamping method to pattern hydrophobic barriers in the filter paper enables rapid fabrication and assembly of microfluidic paper-based analytical devices (μ-PADs). The cut-and-insert assembly method facilitates more efficient fluid transfer than conventional O2 plasma-assisted overlapped channel binding, due to the strong physical contact between connected layers. The liquid transfer starts from the center region where two inserted layers are overlapped, and thus the presented method enables consistent liquid transfer independent of the angles of connected fluidic paths. Consequently, the angles between the connected μ-PAD strips as well as the 3D distance for the fluid transfer can be freely adjusted as needed. Also, multiple strips can be easily connected in series or in parallel. For example, perpendicularly connected bended paper channels guide upward and then lateral liquid flows by capillary action. Three important assays, <I>i.e.</I> nitrite 0 to 2 ppm, pH 1 to 10, and glucose 0 to 0.22 molL<SUP>−1</SUP>, were successfully implemented and measured simultaneously using a device with four strips connected in parallel.</P>
Kwak, Da-Hee,Han, Sang-Beom,Lee, Young-Woo,Park, Hyun-Suk,Choi, In-Ae,Ma, Kyeng-Bae,Kim, Min-Cheol,Kim, Si-Jin,Kim, Do-Hyoung,Sohn, Jung-Inn,Park, Kyung-Won Elsevier 2017 Applied Catalysis B Vol.203 No.-
<P><B>Abstract</B></P> <P>Many alternatives to typical Pt-based catalysts have been developed to enhance oxygen reduction reaction (ORR) performance in acid medium due to their scarcity and high activation loss during the ORR. We synthesized mesoporous carbon nanostructures with multi-dopants such as iron, nitrogen, and sulfur as a cathode catalyst using the ordered silica templates and porphyrinic iron. The co-doped mesoporous carbon cathode catalysts exhibited a high ORR performance in an acid medium, <I>i.e.</I> complete ORR process and improved durability. The enhanced ORR properties of the catalysts might be ascribed to iron-containing catalytic active sites surrounded by nitrogen/sulfur species and a well-defined mesoporous carbon nanostructure.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Doped mesoporous carbon nanostructures were synthesized using a template method. </LI> <LI> The as-prepared samples exhibited a well-ordered mesoporous structure. </LI> <LI> The mesoporous carbon nanostructures contained multi-dopants such as Fe, N, and S. </LI> <LI> The doped carbon nanostructures showed highly improved ORR activity and stability. </LI> <LI> The improved properties were due to a well-doped mesoporous carbon nanostructure. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kwak, Kyu-Won,Kwon, Soon Woo,Nam, Sung-Hee,Park, Kwan-Ho,Kim, Eun-Sun,Lee, Hee-Sam,Choi, Ji-Young,Han, Myung-Sae Korean Society of Sericultural Science 2018 International Journal of Industrial Entomology Vol.36 No.1
The purpose of this study was to determine the best method for minimizing the occurrence of Metarhizium anisopliae infection of Protaetia brevitarsis seluensis during mass breeding on agricultural farms. There is a high demand for the use of P. b. seluensis larvae in animal feed and as food for humans. However, mass breeding results in the entomopathogenic fungal (usually M. anisopliae) infection of P. b. seluensis. A mixture of microorganisms (Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) delayed fungal infection by M. anisopliae, which infected fewer P. b. seluensis when the microorganism mixture was added to sawdust as feed for P. b. seluensis. When sawdust with the effective microorganisms (EM) was given to P. b. seluensis for 30 d, their mortality rate was approximately 35 % less than that of the control group, which was fed sawdust without the EM. In addition, the growth of M. anisopliae on agar media spread with each bacterium as inhibited by up to 80 % more than those spread with 4 % sodium hypochlorite, which is a harmless fungal inhibitor generally used in agricultural farms for disinfection.
Kwak, Cheol Hwan,Kang, Sung-Min,Jung, Euiyoung,Haldorai, Yuvaraj,Han, Young-Kyu,Kim, Woo-Sik,Yu, Taekyung,Huh, Yun Suk THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2018 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.63 No.-
<P><B>Abstract</B></P> <P>A customized droplet-based microfluidic reactor was fabricated for the synthesis of silver nanoparticles (Ag NPs) using silver nitrate (AgNO<SUB>3</SUB>) and branched polyethyleneimine (BPEI) as a precursor and a reducing agent, respectively. The effects of static mixing, temperature, and the volumetric flow rates of AgNO<SUB>3</SUB> and BPEI on the particle size were investigated. The use of a static mixer and the optimization of the reaction temperature enhanced the monodispersity of the Ag NPs. In addition, the size of the Ag NPs was manipulated by changing the flow rate ratio of AgNO<SUB>3</SUB> to BPEI at 60°C for 60min.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Linear facial lichen sclerosus et atrophicus mimicking scleroderma ‘en Coup de Sabre’
( Sang-woo Park ),( Sang-kyung Lee ),( Eui-sung Jung ),( Hyun-bin Kwak ),( Su-kyung Park ),( Jin Park ),( Han-uk Kim ),( Seok-kweon Yun ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1
Localized scleroderma is a inflammatory disease which ultimately leads to a scar-like sclerosis. When linear scleroderma occurs on the forehead, it is referred to as scleroderma ‘en coup de sabre’, given the resemblance of the skin lesions to the stroke of a sabre. We now report an Korean patient who were clinically suspected of ‘en coup de sabre’, but the histologic results confirmed the diagnosis of lichen sclerosus et atrophicus (LSA). A 52-year-old man presented with a several months history of asymptomatic atrophied patch on forehead. He was otherwise healthy, and did not have a family history of autoimmune diseases. There was no past history of trauma. Physical examination revealed linear vertically distributed porcelain white atrophied patch on forehead. The genital, perianal and mucosal areas were normal. A skin biopsy from the lesion showed hyperkeratosis, thinning of the epidermis, loss of the rete ridges, focal basal cell vacuolization, hyalinized collagen of the papillary dermis along with a moderate lymphocytic infiltrate. Based on the clinical and histological findings, this case was diagnosed as linear LSA of the forehead. After the diagnosis was made, the patient was prescribed with topical diflucortolone valerate 0.3% and partial improvement was noted at the 4-months follow up.
( Woo Chul Chung ),( Sung Hoon Jung ),( Kang Moon Lee ),( Chang Nyol Paik ),( Jae Wuk Kwak ),( Ji Han Jung ),( Jin Young Yoo ),( Min Kyoung Lee ),( In Sik Chung ) 대한소화기기능성질환·운동학회 2010 Gut and Liver Vol.4 No.2
Background/Aims: The aim of this study was to determine the structural chromosomal aberrations, such as loss of heterozygosity (LOH) and microsatellite instability (MSI), at multiple tumor suppressor gene loci in gastric epithelial neoplasia categorized by the revised Vienna classification. Methods: All tissue samples were excised by endoscopic mucosal resection. Sixty category 3 (low-grade adenoma) tissue samples and 51 category 4 samples (high-grade adenoma and intramucosal carcinoma with adenoma) were examined at the 7 sets of microsatellite loci linked to the tumor suppressor gene locus. Results: For category 3 and 4 tissue samples, there were no differences in the frequencies of LOH-positive chromosomes or the extent of chromosomal loss. The Helicobacter-pylori (H. pylori)-positive rate was significantly higher in MSI-positive category 4 samples than in category 3 samples (p=0.04). The frequency of MSI positivity was significantly higher in category 4 samples than in category 3 samples (p=0.003). Conclusions: H. pylori infection is associated with genetic instability of the premalignant lesion. MSI occurs in the early stages of gastric carcinogenesis and its occurrence increases during malignant transformation. Detection of MSI in premalignant gastric lesions may be a surveillant of risk of malignant transformation. (Gut Liver 2010;4:179-185)
Porokeratosis at the site of previous moxibustion: a case report
( Sang-woo Park ),( Sang-kyung Lee ),( Eui-sung Jung ),( Hyun-bin Kwak ),( Su-kyung Park ),( Jin Park ),( Seok-kweon Yun ),( Han-uk Kim ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1
Porokeratosis is characterized by annular keratotic rims. The histopathological finding that characterizes this condition is the cornoid lamella, which is a column of parakeratotic cells. It has been suggested that external stimuli, including immunosuppressive therapies, ultraviolet light, and trauma may serve as etiopathogenetic contributors. We report a case of porokeratosis occurring at the site of previous moxibustion. A 59-year-old man visited our clinic with an annular plaque on the right buttock. The plaque showed an atrophic center with a slightly elevated keratotic border. The patient revealed a history of undergoing moxibustion of the lower back and buttocks, 8 years prior to presentation. Unfortunately, he experienced a burn at the site of moxibustion, which was located on the right buttock, and developed a brownish plaque at the site of the burn. The patient was healthy and denied a family history of similar skin lesions at his initial visit. His histopathological examination revealed hyperkeratosis, acanthosis, and cornoid lamella in the epidermis without a granular layer beneath it. Based on the clinical and histopathological findings, the patient was diagnosed with porokeratosis and was treated with 3 cycles of 0.05% ingenol mebutate gel. The lesion decreased in size following 2 weeks of treatment. The residual lesion was treated with carbon dioxide laser, which led to the disappearance of the keratotic annular plaque.