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Study on the Evaluation of Stability of Gel Structured Cosmetics
( Park Chan-ik ),( Kim Ki-sun ),( Lee Sung-jun ),( Yoon Myeong-suk ),( Kang Seh-hoon ) 대한화장품학회 1996 대한화장품학회지 Vol.22 No.2
The stability of gel structured emulsion and the effect of polyols on it have been studied by rheological property and interfacial tension. In this paper, three types of gel structured emulsions were prepared by using three polyols respectively(glycerine for sample 1, 1.3 BG for sample 2, PG for sample 3). And both complex modulus(G*) and loss angle[δ = tan-1 (G“/G‘)] of samples were investigated against oscillating shear stress and frequency(co). The results show sample 1 is most highly consistent with oscillating shear stress. And the results were compared with those of accelerated tests concerning storage stability of gel structured emulsion. To correlate consistency of rheological property with storage stability, interfacial tension from which adsorption efficiency of surfactant(Octyldodecyl Ether) could be known was measured. Sample 1 showed the largest value of [dγ/dln<sub>Cconc. of surfactant</sub>]in Gibbs equation. In summary, the prediction of stability could be correctly made by the consistency of rheological property(G*,γ) of gel structured emulsion against oscillating shear stress and it could be supported by measuring interfacial tension. And polyol affected the value of [dγ/dlnC<sub>conc. of surfactant</sub>], consequently affected the stability.
Sweet ` s Syndrome Associated with Acute Erythema Nodosum
Park, Wook Hwa,Chang, Sung Nam,Youn, Mi Ra,Chun, Soo Il,Haam, Ik Byeong 대한피부과학회 1998 Annals of Dermatology Vol.10 No.3
A 44-year-old man had Sweet's syndrome (acute febrile neutrophilic dermatosis), accompanied by erythematous tender subcutaneous nodules resembling erythema nodosum(EN). The EN-like lesions histologically showed a septal panniculitis with predominantly neutrophilic infiltrates. The association of Sweet's syndrome with EN seems to be uncommon and only a few cases have been reported until the present. We describe a patient with Sweet's syndrome associated with acute EN.
Park, Soon-Ik,Yoe, Sung Moon The Korean Society for Integrative Biology 2012 Animal cells and systems Vol.16 No.6
Amongst the various antimicrobial peptides, lysozyme plays a central role in initiating and maintaining the antibacterial defense response of insect. Here we propose the biosynthesis and refolding of recombinant lysozyme in Escherichia coli expressed in inclusion body form. The Agrius lysozyme gene was amplified using gene specific primers and then ligated into the pGEX-4T-1 vector, which contained the glutathione S-transferase (GST) gene as a fusion partner. A recombinant lysozyme was expressed in E. coli Rosetta cells using a pGEX-4T-1 expression vector, and the fusion protein was induced by ioporpyl-${\beta}$-D-thiogalactopyranoside (IPTG). The recombinant protein produced as an inclusion body was resolubilized in solubilization buffer, and the resultant solution was dialyzed in refolding buffer. After thrombin cleavage, the recombinant lysozyme was purified by ion exchange chromatography and reverse phase chromatography. The recombinant lysozyme was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis and immunoreactivity against the anti-Agrius lysozyme was observed by western blot analysis of this protein. The recombinant lysozyme displayed antibacterial activity against Bacillus megaterium and Micrococcus luteus, which was confirmed by the inhibition zone assay.
참당귀, 일당귀, 중국당귀를 엽록체 InDel 마커의 적용
Sang Ik Park,Jin Su Gil,So Hyeon Park,Yi Lee 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Angelica gigas is a monocarpic perennial plant. A. gigas, also called DangGui or Korean Angelica, is a major medicinal herb used in Asian countries such as Korea, Japan and China. In Korea, we are using the roots of A. gigas. but, Chinese using Angelica sinensis and Japanese using Angelica acutiloba with the same name 'DangGui'. The biggest problem in the use of A. gigas is the confusion with A. acutiloba or A. sinensis. This confusion can cause an medical accident or lack of pharmacological ingredients. In this study, we developed chloroplast InDel markers that can distinguish A. gigas, A. acutiloba or A. sinensis. Methods and Results : We collected 14 Angelica plant samples including A. gigas, A. acutiloba and A. sinensis and extrated DNA using CTAB method. The DNA was diluted to 10 ng/㎕ and kept -20℃. We designed the primer sets using CLC Main Workbench based on chloroplast DNA InDel region of between A. gigas and A. acutiloba. PCR were performed on the 14 Angelica plant samples including A. gigas, A. acutiloba and A. sinensis (5 repeats each). Electrophoresis was performed using fragment analyzer automated CE system. We designed 6 InDel primer sets and the primer sets amplified the amplicons effectively. Three of the 6 primer sets showed polymorphism. Conclusion : We could distinguish A. gigas, A. acutiloba, and A. sinensis using 2 newly developed InDel markers.
The Research of Beach Deformation after Construction of the Jetties
Park, Sang-Kil,Han, Chong-Soo,Roh, Tae-Young,Park, O-Young,Ahn, Ik-Seong,Lee, Ji-Hun Korean Society of Ocean Engineers 2011 International journal of ocean system engineering Vol.1 No.4
This research was described the prevention of coastal topographical change and sediment diffusive concentration incoming from small estuary after construction jetties. This structure is constructed to decrease sediment deposition incoming from the upstream river due to the urbanization and industrial development and to minimize effects on the coastal ecosystem. The physical modeling and numerical modeling for waves were conducted to analyze the configuration of Imrang sand beach deformation without and with construction of jetty. The specification of the installed jetty, which is able to control sedimentation concentration was decided based on the prediction of the Imrang beach area changes by space and time. As a result, the jetties constructed in the estuary retarded the rate of sand sediment, so that the effect area of sand sedimentation was obviously decreased. In addition, the measured field data indicated that the sediment deposition inside of dikes could be controlled and the right side area of jetties could be preserved without sediment deposition.
Mild and Efficient Silylcyanation of Aldehydes Catalyzed by Zirconium Complex
Sung Soo Kim*,Jun Tae Lee,Dae Ho Song,Ik-Mo Lee,Sun Young Park 대한화학회 2005 Bulletin of the Korean Chemical Society Vol.26 No.2
An efficient method of addition of trimethylsilyl cyanide to aldehydes by employing a zirconium complex as the catalyst has been described. A variety of aromatic, aliphatic, cyclic and heterocyclic aldehydes has been converted into corresponding trimethylsilyl ethers in excellent yield within short reaction time.
Identification of Expansin Genes in Platycodon grandiflorum A. Using RNA-seq Analysis
Sang Ik Park,Jemin Yoo,Yurry Um,Yi Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Platycodon grandiflorum A. is a perennial plant belongs to Campanulaceae family. This plant has been used herbal medicine ingredient in East Asia. Because of the high saponin content, it is an economically important medicinal plant in Korea. It has been reported that saponins of P. grandiflorum were mainly synthesized in root tissues. The studies about root growth of the plant were few. Expansin is an important protein playing a role in root growth of plants, and is known as a nonenzymatic protein. Expansins are novel plant cell wall loosening proteins leading to turgor-driven cell extension. Expansin encoding genes exist in multigene family, and there are more than 30 genes in Arabidopsis thaliana. and more than 50 genes in Oryaza sativa. Therefore, identification of the genes was difficult in P. grandiflorum because of the lack of genome sequence. Recently, the development of next generation sequencing (NGS) technologies make it possible to obtain the target genes sequences rapidly and precisely. In this study, to identify the expansin encoding genes in P. grandiflorum, we used RNA-seq analysis with Illumina HiSeq platform. We analyzed whole transcriptome of P. grandiflorum through the RNA-seq analysis based on next generation seuqencing. CLC Genomics Workbench software (Clc Bio inc.) was used for assembly. We assembled 122,663 contigs and search 123 contigs were identified from the search using 61 expansin gene