Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Genetic Diversity of Korean Rice Breeding Parents as Measured by DNA Fingerprinting with Simple Sequence Repeat (SSR) Markers

Song,Moon-Tae,Lee,Jeom-Ho,Lee,Sang-Bok,Cho,Youn-Sang,Ku,Ja-hwan,Seo,Kyoung-In,Choi,Seong-ho,Hwang,Heung-Goo 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

Plant Regeneration and Bulblet Formation of Allium wakegi Araki

Song,Won-seob,Yang,Deok-Chun,Yoon,Jae-Ho,Ryu,Sang-Hyun 한국자원식물학회 2004 Plant Resources Vol.7 No.1

Allium wakegi was cultured shoot tip in the condition of light culture. The Allium wakegi added plant growth regulator was observed of plant regeneration and bulblet formation. Callus Induction and growing rate was the best of 78% when added alone 2,4-D 0.5mg/L. In the formation of shoot, its regeneration rate was 96% when added BA 0.5mg/L in the light culture condition. When BA 0.5mg/L and NAA 0.5mg/L mixed and BA 0.5 mg/L and NAA 1.0mg/L mixed, the rates were 99% and 97% respectively, and these conditions were suitable for forming shoot. In the formation of roots, when added NAA 2.0mg/L in the light culture condition, the regeneration rate was 90.6 % and the roots were abnormal. When added NAA 1.0mg/L, the rate was 82 % and the highest. In the formation of bulbs, when BA 05mg/L and NAA 1.0mg/L mixed, the root generantion and its size in the bulbs was the best compare to other treatment experiments.

Effect of Temperature Associated with Early Growth Stimulus on Shortening of Heading Dates in Rice

Song,Moon-Tae,Lee,Jeom-Ho,Cho,Youn-Sang,Hwang,Hung-Goo 한국자원식물학회 2002 Plant Resources Vol.5 No.2

The heading date is known to be controlled by two kinds of genetic constituent, photosensitivity and basic vegetable phase. For the latter, the effect of temperature in early growth period is critical to determine the shortness of vegetative growth periods in plant s life. A phytotron experiment on 55 rice cultivars, consisting of two ecotypes of rices, indica and japonica, was conducted at high and low temperature treatments at early growth stage to investigate the possible role of plant growth stimulus by high temperature to associate with shortening of heading date. The high temperature during the early growth stage stimulated the rice growth as measured by plant height with much difference of the growth response between indica and japonica. The conclusive finding that these growth stimulus in early growth stage was highly correlated with the acceleration of heading is, more or less, correlated with the heading of the late growth stage although we could not conclude the genes for early plant growth stimulus by high temperature is the same genes as the genes for accelerating of heading in the late growth stage of plants.

Streptomyces 속 KM-48 균주가 생성하는 Bacillus amyloliquefaciens 에 대한 항생물질

Lee, Sang-Man,Song, Bang-Ho 한국미생물 · 생명공학회 1983 한국미생물·생명공학회지 Vol.11 No.1

세포외 효소를 분필하는 Bacillus amyloliquefaciens의 생육을 선택적으로 저해하는 항생물질을 분리하고저 대구 근교의 토양시료 100여점을 채취하여 Streptomyces sp. KM-48주를 최종 선별하였다. 이 균주의 Nutrient Broth 배지에서의 배양00으로부터 활성성분을 acetone 유출, $Al_2$O$_3$, chromatography, avical TLC를 하여 UV 조사하에서 청록색 형광을 나타내는 단일물질로 정제하였다. 이 물질은 주로 그람양성균과 균모, 곰팡이 등에 강한 항균효과를 나타내었으며 Bacillus amyloliquefaciens에 대한 50% 생육저해농도가 18$\mu\textrm{g}$/$m\ell$이었다. 이 물질은 열에 비교적 약하였으며 산에는 강하게 그 활성이 유지되었다. 균주의 형태 및 배양성에서 Stretommces tsusimaensis의 유이균으로 동정되었다. One of Streptomyces sp. was screened as an antibiotic producing strain against Gram positive bacteria specifically from about one hundred Actinomycetes collections. The isolated KM-48 strain, indexed in our collection, was secreted an anbitiotic in nutrient broth culture. The active principle was extracted with acetone from the lyophilized solid of cultured filtrate. The nonadsorbed elute in aluminium oxide was isolated as a purified homogeneity which revealed a sing1e spot under ultra-violet beam on avicel thin layer chromatography. The isolated antibiotic was sensitive specifically against the Gram positive bacteria and fungi. About fifty percent inhibition of the control growth on Bacillus amyloliquefaciens was 18$\mu\textrm{g}$/$m\ell$. The antibiotic was lavile against thermotreatment and stabled in acidic condition remarkably. The KM-48 was identified as a similar strain to Streptomyces tsusimaensis on its morphological and cultural characteristics.

경부고속철도건설사업 성과평가

송성태(Sung Tae Song),최문규(Moon Kyu Choi),박상구(Sang Gu Park),장호환(Ho Hwan Jang),차효정(Hyo Jung Cha) 한국철도학회 2012 한국철도학회 학술발표대회논문집 Vol.2012 No.5

1992년 첫 삽을 뜨면서 시작한 경부고속철도건설사업이 2004년 4월, 1단계 개통을 한데 이어 지난 2010년 11월 완전 개통하였다. 경부고속철도 개통으로 경부축 지역의 2시간대 서비스 제공을 통한 국토의 균형발전 촉진 및 여가·관광사업 활성화, 기술발전, 환경비용 및 에너지 절감 등 긍정적인 효과를 이룬 반면, 잦은 기본계획 변경으로 인한 사업비 및 사업기간의 증가, 현실과 맞지 않은 설치기준으로 인한 과잉설계 문제 등 부정적 효과도 초래했다. 본 연구를 통해 사업추진 단계별 주요 이슈에 대해 객관적인 시각으로 문제점을 분석하고 시사점을 도출함으로써, 현재 추진하고 있는 호남고속철도와 수도권고속철도 사업에서는 시행착오를 줄이고 사업을 효율적으로 추진하는데 활용하고자 한다. Launched in 1992, the Gyeung-Bu High Speed Railway (HSR) was fully opened in November 2010 following the partial opening of the first phase in April 2004. Since the opening it has made tremendous achievements, including but not limited to, technical innovation, balanced regional development, growth of leisure and tourism industries, and savings of environmental costs and energy. On the other hand, it has brought negative effects such as increase in project time and cost by frequent changes of the basic plan and over-design based on unrealistic criteria. In this study we will analyze major issues that occurred at each stage of the Gyeong-Bu HSR project and draw implications. Thus, this study will enhance to efficiently implement the Ho-Nam and Seoul Metropolitan HSR project through minimizing possible trials and errors.

유산소성 운동이 노인들의 신체조성과 등속성 슬관절 근력 및 요부근력에 미치는 영향

한종우(Jong Woo Han),이상석(Sang Sug Lee),송분도(Bun Do Song),지용석(Yong Suk Ji),김난수(Nan Su Kim),서경호(Kyeong Ho Seo) 한국사회체육학회 2000 한국사회체육학회지 Vol.13 No.-

This study was designed to investigate the effect of a year aerobic exercise training on body composition, isokinetic knee and trunk muscular strength of elders. The subjects for this study were eighty healthy elderly people who were members of seniors tower at Song-Do Hospital and didn`t have any history of cardiorepiratory disease. Subjects have been trained three times a week for a year. The exercise program was made up of a 15-minute warm-up, cool-down. Stretching was performed every eight minutes for the prevention of injuries which could occur during the main exercise and quick recovery of the used muscle. Aerobic training was performed for each 15-20minutes using the treadmill and cycle ergometer at the intensity of 50-70% HRmax. For the data, all the measurements were represented by mean and standard deviation using SAS package(version 6.12). Statistical techniques for data analysis were paired t-test to determine the difference between pre and post exercise program. The 5% level of significance was used as the critical level for acceptance of hypotheses for the study. The following results were obtained from this study. 1. There is a statistically significant difference in the VO_2max, fat mass, lean mass and %body fat both old men and women after a year training. 2. There is not a statistically significant difference in the weight, WHR and thigh circumference both old men and women after a year training. 3. There is a statistically significant difference in the right knee flexor/extensor peak torque, peak torque % body weight, total work and total work % body weight both old men and women after a year training. 4. There is a statistically significant difference in the left knee flexor/extensor peak torque, peak torque % body weight, total work and total work % body weight both old men and women after a year training.

Morin exerts cytoprotective effects against oxidative stress in C2C12 myoblasts via the upregulation of Nrf2-dependent HO-1 expression and the activation of the ERK pathway

Lee, Moon Hee,Han, Min Ho,Lee, Dae-Sung,Park, Cheol,Hong, Su-Hyun,Kim, Gi-Young,Hong, Sang Hoon,Song, Kyoung Seob,Choi, Il-Whan,Cha, Hee-Jae,Choi, Yung Hyun UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.39 No.2

<P>In the present study, we investigated the cytoprotective efficacy of morin, a natural flavonoid, against oxidative stress and elucidated the underlying mechanisms in C2C12 myoblasts. Our results indicated that morin treatment prior to hydrogen peroxide (H2O2) exposure significantly increased cell viability and prevented the generation of reactive oxygen species. H2O2-induced comet-like DNA formation and gamma H2AX phosphorylation were also markedly suppressed by morin with a parallel inhibition of apoptosis in C2C12 myoblasts, suggesting that morin prevented H2O2-induced cellular DNA damage. Furthermore, morin markedly enhanced the expression of heme oxygenase-1 (HO-1) associated with the induction and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the inhibition of Kelch-like ECH-associated protein 1 (Keapl) expression. Notably, these events were eliminated by transient transfection with Nrf2-specific small interfering RNA. Additional experiments demonstrated that the activation of the Nrf2/HO-1 pathway by morin was mediated by the extracellular signal-regulated kinase (ERK) signaling cascade. This phenomenon was confirmed with suppressed Nrf2 phosphorylation and consequently diminished HO-1 expression in cells treated with a pharmacological inhibitor of ERK. Collectively, these results demonstrated that morin augments the cellular antioxidant defense capacity through the activation of Nrf2/HO-1 signaling, which involves the activation of the ERK pathway, thereby protecting C2C12 myoblasts from H2O2,-induced oxidative cytotoxicity.</P>

Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

Lee, Eun-Mi,Lee, Young-Eun,Lee, Esder,Ryu, Gyeong Ryul,Ko, Seung-Hyun,Moon, Sung-Dae,Song, Ki-Ho,Ahn, Yu-Bae Korean Diabetes Association 2011 Diabetes and Metabolism Journal Vol.35 No.5

<P><B>Background</B></P><P>Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic β-cells from high glucose-induced apoptosis.</P><P><B>Methods</B></P><P>Reverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations.</P><P><B>Results</B></P><P>CoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP.</P><P><B>Conclusion</B></P><P>Our data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.</P>

A Case of von Hippel-Lindau Disease with Aortic Valve Insufficiency

Sang Hyeon Kang,In Chul Park,Duk Song Cho,Hye Jung Lee,Ho Jin Lee,Dong Hyun Lee 영남대학교 의과대학 2013 Yeungnam University Journal of Medicine Vol.30 No.2

Von Hippel-Lindau (VHL) disease is an autosomal dominant hereditary disorder caused by a germline mutation of the VHL gene. It is a multi-systemic disorder that is predisposed to benign or malignant tumors of visceral organs such as hemangioblastoma of the central nervous system, renal cell carcinoma, retinal angioma and pheochromocytoma. We report herein a case of VHL disease that initially manifested with aortic valve insufficiency.