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Suel Ki Lim,Byeong Cheol Yoon,Dong IL Kim,Seong Soo Kang,Ho Jae Han,Soo Hyun Park 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.3
Liver transplantation is essential for patients with liver failure. Pig is known to be a most suitable species in xeno-transplantation of human organs. Hepatocytes are important sites in the body's metabolism and function. Glucose transporter in hepatocytes plays a role in regulation of blood glucose homeostasis. Epidermal growth factor (EGF) is known to be a regulator of physiological function in various cell systems. However, the effect of EGF on glucose activity in pig hepatocytes was not clearly elucidated. Thus, this study investigated the effect of EGF on glucose transporter and its related signal cascades in primary cultured pig hepatocytes. EGF inhibited glucose uptake in a dose (> 1 ng/㎖) dependent manner. EGF-induced decrease of [³H]-deoxyglucose uptake was blocked by AG 1478 (10-⁶ M, an EGF receptor antagonist) genistein and herbymycin A (tyrosine kinase inhibitors, 10⁻⁶ M), suggesting the role of activation and tyrosine phosphorylation of EGF receptor. In addition, EGF-induced decrease of [³H]-deoxyglucose uptake was prevented by mepacrine, AACOCF₃ (phospholipase A₂ inhibitors), indomethacin and ibuprofen (cyclooxygenase inhibitors). Indeed, EGF increased [³H]-arachidonic acid release and cyclooxygenase-2 (COX-2) expression. Moreover, EGF-induced stimulation of [³H]-arachidonic acid release was blocked by PD 98059 (a p44/42 mitogen activated protein kinase [MAPK] inhibitor) and SB 203580 (a p38 MAPK inhibitor). EGF-induced decrease of [³H]-deoxyglucose uptake was also prevented by these two MAPKs. However, EGF-induced stimulation of p44/42 MAPK and p38 MAPK was not blocked by mepacrine and indomethacin. In conclusion, EGF decreases glucose uptake via MAPK-COX-2 signal cascades in cultured pig hepatocytes.
Fiber-Optic Refractive Index Sensor Based on the Cone-Based Round Structure
Hyeon-Ho Jeong,Yoo-Jin Son,Suel-Ki Kang,Hye-Jin Kim,Hyun-Jeong Roh,Erdene, N.,Jae-Hyoung Park,Dae-Hong Jeong,Ho-Young Lee,Seung-Ki Lee IEEE 2013 IEEE SENSORS JOURNAL Vol.13 No.1
<P>Fiber-optic refractive index (RI) sensors have the advantages of low cost, simple optical setup, remote sensing, and simple fabrication. A cone-based round structure, which is the combined structure of the cone structure and the round structure, is successively fabricated by using a cleaving method, a chemical glass etching method, and an electrical arc discharge method. In order to examine the possibility of the RI sensor, the fabricated cone-based round structure is used for the detection of various RIs by measuring the reflected intensities in the target sensing media. In addition, a fiber-optic localized surface plasmon resonance (LSPR) sensor based on the cone-based round structure is fabricated to enhance the sensitivity regarding RI changes. The fabricated fiber-optic LSPR sensor is used to detect the change of the RIs using the shift of peak wavelengths, as well as the change of reflected resonance intensities, by measuring LSPR spectra for gold nanoparticles on it.</P>
Byeong Cheol Yoon,Min Jung Park,Suel Ki Lim,Seong Soo Kang,Ho Jae Han,Soo Hyun Park 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.3
Miniature pig is known to be a most suitable species in xeno-transplantation of human organs. Autonomic nervous system coupled with G protein plays a pivotal role in the regulation of digestive system. The physiological role of autonomic nervous system may be linked to the interaction of its receptors. However, the distribution of adrenoceptors and muscarinic receptors in digestive system was not known in miniature pig. Thus we examined the expression of adrenoceptors (α1, α2, β1, and β2) and muscarinic receptors (M1, M2, M3) mRNA in the digestive organs of miniature pigs. In the present study, α1, α2, β1, M2, and M3 mRNA expression was evident in stomach but β2 and M1 mRNA expression was not shown in stomach. α1 receptor gene expression was relative high in small intestine and α2 receptor gene expression in large intestine. In addition, β1 receptor mRNA expression was restricted in duodenum, ileum, jejunum and cecum but was not shown in colon and rectum. However, β2 receptor mRNA expression was not evident in these tissues. M1 mRNA expression was evident within in duodenum and ileum, M2 mRNA expression in stomach and small intestine, and M3 mRNA expression in all tissues. Moreover, α1, α2, β1 and M3 was evident in pancreas. In conclusion, adrenoceptors and muscarinic receptors are tissue-specifically expressed in digestive organs of miniature pigs.