The Effect of Non-Fermented and Fermented Soymilk (Glycine Max) on Liver Malondialdehyde Levels in the Dyslipidemic Rats (Rattus Norvegicus)

( Hilmi Ardian Sudiarto ),( Dini Islamiana ),( Miranti Dewi Pramaningtyas ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Liver is an organ that plays an important role in lipid metabolism. The accumulation of lipids can increase the level of reactive oxygen species (ROS). Malondialdehyde (MDA) can be used as a marker of oxidative stress. If this occur continuously, it will increase the risk of non alcoholic fatty liver disease. There were few studies have compared the effect of non-fermented and fermented soymilk on dyslipidemia. The purpose of this research is to compare the effect of non-fermented and fermented soymilk on liver MDA levels of dyslipidemic rats. Methods: This study used a quasi-experimental method with post-test only control group design. This research was conducted in the laboratory of physiology, Universitas Islam Indonesia (UII) for 4 weeks. This research used male Wistar strain rats aged 1-2 months with BW of 100-150 grams. Rats were divided into four groups. All groups were given fed ad libitum for 4 weeks. In the first two weeks, first group, third group, and fourth group were given 5 ml/200 gram BW/day quail egg yolks (G1, G3, and G4), while second group was not given the quail egg yolks (G2). In the second two weeks, third group (G3) were given 5 ml/200 gram BW/day non fermented soymilk, while fourth group (G4) were given 5 ml/200 gram BW/ day fermented soymilk. In the end of the research, MDA levels on liver were measured. ANOVA with bonferroni post-hoc test was used in statistical analyzing. Results: Mean of MDA level (nmol/gr) were 1,96 ± 0,34 for G1; 9,70 ± 0,24 for G2, 4,60 ± 0,27 for G3, and 3,38 ± 0,32 for G4. Statistical analyzing shown that there were significant differences of MDA levels among the groups (P=0.00). Conclusions: Both non fermented and fermented soymilk had the potency to reduce MDA levels (P=0.00), but fermented soymilk can reduce the MDA level on liver better than non-fermented soymilk.

The Relationship between Total Cholesterol (TC) Levels and Lipid Fraction Area of Liver Tissue in the Dyslipidemic Rats (Rattus Norvegicus) Model after Intervention of Probiotic Beverage from Date Palm and Kefir Milk

( Hilmi Ardian Sudiarto ),( Rafik Prabowo ),( Miranti Dewi Pramaningtyas ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Dyslipidemia is often associated with the occurrence of fatty liver disease. One of the signs of dyslipidemia is an increase in total cholesterol (TC) levels, while fatty liver disease is characterized by the accumulation of lipids in hepatocytes. The purpose of this research is to know the correlation between TC and lipid fraction area of liver tissue in the dyslipidemic rats (Rattus norvegicus) model after the intervention of probiotic beverage from date palm and kefir milk. Methods: This study used a quasi-experimental method with post-test only control group design. This research was conducted in the laboratory of physiology, Universitas Islam Indonesia (UII) for 2 months. This research used male Wistar strain rats aged 1-2 months with BW of 100-150 grams. Rats were divided into three groups. All groups were given fed ad libitum for 2 months. In the first month, the first group and third group were given 5 ml/200 gram BW/day quail egg yolks (G1 and G3), while the second group was not given the quail egg yolks (G2). In the second month, the third group was given 5 ml/200 gram BW/day probiotic beverage from date palm and kefir milk. At the end of the research, rats were terminated. Results: The mean of TC (mg/dL) in G1, G2, and G3 consecutively were 70.91 ± 3.21, 174.71 ± 3.25, and 125.67 ± 4.69. The mean of lipid fraction area (%) in G1, G2, and G3 consecutively were 0.95 ± 0.17, 1.50 ± 1.34, and 1.43 ± 0.53. The result showed there is a normal correlation between TC and lipid fraction area of liver tissue with r 0.541 (normal positive correlation) and no significant difference with P>0.05 (P=0.069). Conclusions: TC and lipid fraction area of liver tissue in dyslipidemic rats after the intervention of probiotic beverage from date palm and kefir milk have a normal correlation with no significant difference.

The Relationship between Serum Low-Density Lipoprotein (LDL) and Lipid Fraction Area in The Dyslipidemic Rat’s Liver after Intervention of Probiotic Beverage from Date Palm and Kefir Milk

( Hilmi Ardian Sudiarto ),( Rafik Prabowo ),( Miranti Dewi Pramaningtyas ) 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1

The Effect of Non-Fermented and Fermented Soymilk (Glycine max) on Glucose Levels in The Dyslipidemic Rats (Rattus norvegicus) Model

( Hilmi Ardian Sudiarto ),( Rafik Prabowo ),( Miranti Dewi Pramaningtyas ) 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1

The investigation of combined ventilation-biofilter systems using recycled treated wastewater on odor reduction efficiency

FEBRISIANTOSAANDI,최홍림,Renggaman Anriansyah,Sudiarto Sartika I. A.,Lee Joonhee 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.7

Objective: The present study aimed to evaluate the performance of odor abatement by using two different ventilation-biofilter systems with recycled stablized swine wastewater. Methods: The performance of odor removal efficiency was evaluated using two different ventilation-biofilter-recycled wastewater arrangements. A recirculating air-flow ventilation system connected to a vertical biofilter (M1) and a plug-flow ventilation system connected to a horizontal biofilter (M2) were installed. Water dripping over the surface of the biofilter was recycled at a flow rate of 0.83 L/h in summer and 0.58 L/h in winter to reduce odorous compounds and particulate matter (PM). The experiments were performed for 64 days with M1 and M2 to investigate how these two ventilation-biofilter systems influenced the reduction of odor compounds in the model houses. Odorous compounds, NH3 and volatile organic compounds (VOCs) were analyzed, and microclimatic variables such as temperature, humidity, and PM were monitored. Results: Ammonia concentration inside M1 was about 41% higher on average than that in M2. PM and total suspended particles (TSPs) inside M1 were about 62.2% and 69.9%, respectively, higher than those in M2. TSPs in the model house were positively correlated with the concentration of NH3 and VOCs. Conclusion: M2 emitted lower concentration of odorous compounds than M1. Moreover, M2 could maintain the optimum temperature condition for a swine house during the cooler season. The plug-flow ventilation–horizontal biofilter system could be used for pig houses to minimize air pollution produced by swine farming activities and maintain optimum microclimate conditions for pigs.

Prediction of livestock manure and mixture higher heating value based on fundamental analysis

Choi, Hong L.,Sudiarto, Sartika I.A.,Renggaman, Anriansyah Elsevier 2014 Fuel Vol.116 No.-

<P><B>Abstract</B></P> <P>The ongoing availability of livestock waste presents an opportunity for its utilization as renewable energy resource through biological or thermochemical conversion. However, the conversion efficiency and the potential energy content of livestock waste needs to be estimated in order to design such a renewable energy production system. To address this, eight types of livestock waste were collected from each of 12 commercial farms from April to May 2009. The higher heating value (HHV), which represents the actual energy content, along with characteristics of livestock waste was determined. Moreover, equations for estimating HHV from proximate, ultimate, and chemical analysis were established by regression analysis. The developed equations were then validated using additional livestock waste data. The HHV of livestock waste was found to be in the range from 11.92 to 19.44MJ/kg dry matter. The equation, HHV=0.1970VM+0.3955 represents the best-fit equations derived from proximate analysis with an Average absolute error (AAE) value of 9.17%. The equation, HHV=0.1865CH+0.2671PR+0.2141F −0.2151 is best-fit equation derived from chemical analysis with an AAE value of 5.31%. The equation derived in this study, HHV=0.3198C+0.0803O+0.4704N −1.4502S+0.9364, was compared with ten recently published correlations based on ultimate analysis and showed better accuracy by having the lowest AAE values of 8.57%. All developed equations can be used to estimate HHV of various livestock waste with the exception of swine manure. The main reason for this limitation arises from the unique characteristics of pig manure in comparison to other livestock waste found in this study.</P> <P><B>Highlights</B></P> <P> <UL> <LI> HHV and characteristics of various livestock manure and mixture were determined. </LI> <LI> HHV correlations with livestock manures and mixtures characteristics were examined. </LI> <LI> Lower AAE value was obtained when pig manure excluded from equations database. </LI> <LI> Proposed equations give better accuracy than previous published equations. </LI> </UL> </P>

Open Access : Development of pig welfare assessment protocol integrating animal-, environment-, and management-based measures

( Anriansyah Renggaman ),( Hong L Choi ),( Sartika Ia Sudiarto ),( Laura Alasaarela ),( Ok S Nam1 ) 한국동물자원과학회(구 한국축산학회) 2015 한국축산학회지 Vol.57 No.1

Background: Due to increased interest in animal welfare, there is now a need for a comprehensive assessment protocol to be used in intensive pig farming systems. There are two current welfare assessment protocols for pigs: Welfare Quality® Assessment Protocols (applicable in the Europe Union), that mostly focuses on animal-based measures, and the Swine Welfare Assurance Program (applicable in the United States), that mostly focuses on management- and environment-based measures. In certain cases, however, animal-based measures might not be adequate for properly assessing pig welfare status. Similarly, welfare assessment that relies only on environment- and management-based measures might not represent the actual welfare status of pigs. Therefore, the objective of this paper was to develop a new welfare protocol by integrating animal-, environment-, and management-based measures. The background for selection of certain welfare criteria and modification of the scoring systems from existing welfare assessment protocols are described. Methods: The developed pig welfare assessment protocol consists of 17 criteria that are related to four main principles of welfare (good feeding, good housing, good health, and appropriate behavior). Good feeding, good housing, and good health were assessed using a 3-point scale: 0 (good welfare), 1 (moderate welfare), and 2 (poor welfare). In certain cases, only a 2-point scale was used: 0 (certain condition is present) or 2 (certain condition is absent). Appropriate behavior was assessed by scan sampling of positive and negative social behaviors based on qualitative behavior assessment and human-animal relationship tests. Results: Modification of the body condition score into a 3-point scale revealed pigs with a moderate body condition (score 1). Moreover, additional criteria such as feed quality confirmed that farms had moderate (score 1) or poor feed quality (score 2), especially those farms located in a high relative humidity region. Conclusions: The developed protocol can be utilized to assess welfare status in an intensive pig farming system. Although further improvements are still needed, this study is a first step in developing a pig welfare assessment protocol that combines animal-, environment-, and management-based measures.