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      • KCI등재

        Lateral alveolar ridge augmentation procedure using subperiosteal tunneling technique: a pilot study

        Kakar, Ashish,Kakar, Kanupriya,Sripathi Rao, Bappanadu H.,Lindner, Annette,Nagursky, Heiner,Jain, Gaurav,Patney, Aditya Korean Association of Maxillofacial Plastic and Re 2018 Maxillofacial Plastic Reconstructive Surgery Vol.40 No.-

        Background: In this research article, we evaluate the use of sub-periosteal tunneling (tunnel technique) combined with alloplastic in situ hardening biphasic calcium phosphate (BCP, a compound of β-tricalcium phosphate and hydroxyapatite) bone graft for lateral augmentation of a deficient alveolar ridge. Methods: A total of 9 patients with deficient mandibular alveolar ridges were included in the present pilot study. Ten lateral ridge augmentation were carried out using the sub-periosteal tunneling technique, including a bilateral procedure in one patient. The increase in ridge width was assessed using CBCT evaluation of the ridge preoperatively and at 4 months postoperatively. Histological assessment of the quality of bone formation was also carried out with bone cores obtained at the implant placement re-entry in one patient. Results: The mean bucco-lingual ridge width increased in average from 4.17 ± 0.99 mm to 8.56 ± 1.93 mm after lateral bone augmentation with easy-graft CRYSTAL using the tunneling technique. The gain in ridge width was statistically highly significant (p = 0.0019). Histomorphometric assessment of two bone cores obtained at the time of implant placement from one patient revealed 27.6% new bone and an overall mineralized fraction of 72.3% in the grafted area 4 months after the bone grafting was carried out. Conclusions: Within the limits of this pilot study, it can be concluded that sub-periosteal tunneling technique using in situ hardening biphasic calcium phosphate is a valuable option for lateral ridge augmentation to allow implant placement in deficient alveolar ridges. Further prospective randomized clinical trials will be necessary to assess its performance in comparison to conventional ridge augmentation procedures.

      • KCI등재

        A systematic documentation on a rare medicinal plant Acacia ferruginea: a ready reckoner

        M. Jeevitha,Shubashini K. Sripathi 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.4

        The key ingredients in a wide range of Ayurvedic, Siddha, and homeopathic medications are medicinal plants which have an impact on the treatment wide range of ailments and disorders while also contributing to humanity’s overall health. To obtain commercial value and worldwide acceptance, herbal medications must contain standardized extracts. Documenting the physico-chemical, chemical, and biological features of legitimate medicinal plants/extracts are one of the existing standardization procedures. Acacia, a large genus housing about 160 species, belongs to the Fabaceae family. Acacia ferruginea, a potential herb with medicinal utilization is endemic to the tropical region. Plants have been exploited as a viable medicine formulation source due to advances in phytochemistry over the last few decades. The present documentation intends to recapitulate detailed information on the plant comprising morphological features, physico-chemical characteristics, phytochemical composition and pharmaceutical properties reported through scientific validation and along with its cultivation and conservation challenges. The goal of this study is to detail the medical benefits and phytochemical potential of Acacia ferruginea, and conscientious efforts are needed to explore its benefits and validation of its traditional uses through clinical applications. The current documentation robustly covers the literature on A. ferruginea from 1965 to Dec 2021 and also includes findings of our recent studies on this plant. Providing up-to-date information on its botanical aspects for easy recognition of the plant, as well as its historical background/origin along with our original findings and the assessment comprises investigations that have been on the plant’s numerous qualities and activities. The presence of flavonoids, alkaloids, phenolics, tannins, and saponins is revealed by qualitative/quantitative studies. The bark of the plant has been extensively studied and the plant possesses antioxidant, anti-cancer, anti-diabetic, anti-bacterial, anti-fungal, anti-haemorrhoidal, and larvicidal properties. This precise record of details pertaining to standardization of extracts of A. ferruginea will assist producers of natural formulations as a ready reckoner.

      • KCI등재후보

        Acibenzolar-S-Methyl (ASM)-Induced Resistance against Tobamoviruses Involves Induction of RNADependent RNA Polymerase (RdRp) and Alternative Oxidase (AOX) Genes

        Kallahally Nagendra Madhusudhan,Saligrama Adavigowda Deepak,Harishchandra Sripathi Prakash,Ganesh Kumar Agrawal,Nam Soo Jwa,Randeep Rakwal 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.2

        Tobamoviruses are the major viral pathogens of tomato and bell pepper. The preliminary results showed that Acibenzolar-Smethyl (ASM; S-methylbenzo (1,2,3) thiadiazole-7-carbothiate) pre-treatment to tomato and tobacco plants reduces the concentration of Tomato mosaic tobamovirus (ToMV) and Tobacco mosaic tobamovirus (TMV) in tomato and bell pepper seedlings, respectively. Pre-treatment of the indicator plant (Nicotiana glutinosa) with the ASM followed by challenge inoculation with tobamoviruses produced a reduced number and size of local lesions (67 and 79% protection over control to TMV and ToMV inoculation, respectively). In order to understand the mechanism of resistance the gene expression profiles of antiviral genes was examined. RT-PCR products showed higher expression of two viral resistance genes viz., alternative oxidase (AOX) and RNA dependent RNA polymerase (RdRp) in the upper leaves of the ASM-treated tomato plants challenge inoculation with ToMV. Further, the viral concentration was also quantified in the upper leaves by reverse transcription PCR using specific primer for movement protein of ToMV, as well as ELISA by using antisera against tobamoviruses. The results provided additional evidence that ASM pre-treatment reduced the viral movement to upper leaves. The results suggest that expressions of viral resistance genes in the host are the key component in the resistance against ToMV in the inducer-treated tomato plants. Tobamoviruses are the major viral pathogens of tomato and bell pepper. The preliminary results showed that Acibenzolar-Smethyl (ASM; S-methylbenzo (1,2,3) thiadiazole-7-carbothiate) pre-treatment to tomato and tobacco plants reduces the concentration of Tomato mosaic tobamovirus (ToMV) and Tobacco mosaic tobamovirus (TMV) in tomato and bell pepper seedlings, respectively. Pre-treatment of the indicator plant (Nicotiana glutinosa) with the ASM followed by challenge inoculation with tobamoviruses produced a reduced number and size of local lesions (67 and 79% protection over control to TMV and ToMV inoculation, respectively). In order to understand the mechanism of resistance the gene expression profiles of antiviral genes was examined. RT-PCR products showed higher expression of two viral resistance genes viz., alternative oxidase (AOX) and RNA dependent RNA polymerase (RdRp) in the upper leaves of the ASM-treated tomato plants challenge inoculation with ToMV. Further, the viral concentration was also quantified in the upper leaves by reverse transcription PCR using specific primer for movement protein of ToMV, as well as ELISA by using antisera against tobamoviruses. The results provided additional evidence that ASM pre-treatment reduced the viral movement to upper leaves. The results suggest that expressions of viral resistance genes in the host are the key component in the resistance against ToMV in the inducer-treated tomato plants.

      • KCI등재

        Classification and surgical management of temporomandibular joint ankylosis: a review

        Varsha Haridas Upadya,Hari Kishore Bhat,B.H. Sripathi Rao,Srinivas Gosla Reddy 대한구강악안면외과학회 2021 대한구강악안면외과학회지 Vol.47 No.4

        The paper reviews various classifications and surgical techniques for the treatment of temporomandibular joint ankylosis. PubMed, EBSCO, Web of Science, and Google Scholar were searched using a combination of keywords. Articles related to classification, resection-reconstruction of the temporomandibular joint, and management of airway obstruction were considered and categorized based on the objectives. Seventy-nine articles were selected, which included randomized clinical trials, non-randomized controlled cohort studies, and case series. Though several classifications exist, most classifications are centered on the radiographic extent of the ankylotic mass and do not include the clinical and functional parameters. Hence there is a need for a comprehensive staging system that takes into consideration the age of the patient, severity of the disease, clinical, functional, and radiographic findings. Staging the disease will help the clinician to adopt a holistic approach in treating these patients. Interpositional arthroplasty (IA) results in better maximal incisal opening compared with gap arthroplasty, with no significant difference in recurrent rates. Distraction osteogenesis (DO) is emerging as a popular technique for the restoration of symmetry and function as well as for relieving airway obstruction. IA, with a costochondral graft, is recommended in growing patients and may be combined with or preceded by DO in cases of severe airway obstruction. Alloplastic total joint replacement combined with fat grafts and simultaneous osteotomy procedures are gaining popularity. A custom-made total joint prosthesis using CAD/CAM can efficiently overcome the shortcomings of stock prostheses.

      • KCI등재

        Acibenzolar-S-Methyl(ASM)-Induced Resistance against Tobamoviruses Involves Induction of RNA-Dependent RNA Polymerase(RdRp) and Alternative Oxidase(AOX) Genes

        Madhusudhan, Kallahally Nagendra,Deepak, Saligrama Adavigowda,Prakash, Harishchandra Sripathi,Agrawal, Ganesh Kumar,Jwa, Nam-Soo,Rakwal, Randeep The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.2

        Tobamoviruses are the major viral pathogens of tomato and bell pepper. The preliminary results showed that Acibenzolar-Smethyl(ASM; S-methylbenzo(1,2,3) thiadiazole-7-carbothiate) pre-treatment to tomato and tobacco plants reduces the concentration of Tomato mosaic tobamovirus(ToMV) and Tobacco mosaic tobamovirus(TMV) in tomato and bell pepper seedlings, respectively. Pre-treatment of the indicator plant(Nicotiana glutinosa) with the ASM followed by challenge inoculation with tobamoviruses produced a reduced number and size of local lesions(67 and 79% protection over control to TMV and ToMV inoculation, respectively). In order to understand the mechanism of resistance the gene expression profiles of antiviral genes was examined. RT-PCR products showed higher expression of two viral resistance genes viz., alternative oxidase(AOX) and RNA dependent RNA polymerase(RdRp) in the upper leaves of the ASM-treated tomato plants challenge inoculation with ToMV. Further, the viral concentration was also quantified in the upper leaves by reverse transcription PCR using specific primer for movement protein of ToMV, as well as ELISA by using antisera against tobamoviruses. The results provided additional evidence that ASM pre-treatment reduced the viral movement to upper leaves. The results suggest that expressions of viral resistance genes in the host are the key component in the resistance against ToMV in the inducer-treated tomato plants.

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