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Growth of Escherichia coli in Iron-enriched Medium Increases HPI Catalase Activity
Zaid, Tarrik,Srikumar, Trivandrum Sukumaran Nair,Benov, Ludmil Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.6
Escherichia coli has two catalases, HPI and HPII. HPI is induced during logarithmic growth in response to low concentrations of hydrogen peroxide. This induction is OxyR-dependent. On the other hand, HPII is not peroxide-inducible but is induced in entry to the stationary phase. We demonstrate here that E. coli displayed higher HPI catalase activity when compared to the cultures that were grown in a normal medium, if grown in a medium supplemented with iron-citrate. Iron supplementation had no effect on HPII catalase. This increase of HPI activity was OxyR-independent and not observed in a ${\Delta}fur$ mutant. The physiological significance of the increase of HPI activity is unclear, but it appears that the katG gene that codes for HPI catalase is among the genes that are regulated by Fur.
Growth of Escherichia coli in Iron-enriched Medium Increases HPI Catalase Activity
( Tarrik Zaid ),( Trivandrum Sukumaran Nair Srikumar ),( Ludmil Benov ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.6
Escherichia coli has two catalases, HPI and HPII. HPI is induced during logarithmic growth in response to low concentrations of hydrogen peroxide. This induction is OxyR-dependent. On the other hand, HPII is not peroxide-inducible but is induced in entry to the stationary phase. We demonstrate here that E. coli displayed higher HPI catalase activity when compared to the cultures that were grown in a normal medium, if grown in a medium supplemented with iron-citrate. Iron supplementation had no effect on HPII catalase. This increase of HPI activity was OxyR-independent and not observed in a Afur mutant. The physiological significance of the increase of HPI activity is unclear, but it appears that the katG gene that codes for HPI catalase is among the genes that are regulated by Fur.
Madupu Hemanth Sai,Chinda Padmanabha Raju,Kotni SriKumar 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.4
In recent years, the addition of renewable-based distribution generation (DG) into the electrical distribution system has accelerated. The power requirements at the load centre can be met more efficiently by distributed generation (DG) than by the conventional power system. DG installation provides many technical benefits which include lower distribution losses, lower voltage stability index, and improved voltage profile. In electrical distribution networks, loads are typically expressed as a constant power (CP) load model, which is not considered realistic. For realistic load modeling of the radial distribution system, optimum load mix is represented as combination of different load models. Because the R/X ratio is so large in radial distribution systems, real power losses are a major concern. This paper presents a tunicate swarm algorithm (TSA) optimization technique for optimal DG installation with various load models and load growth for improved voltage profile, network losses reduction, and voltage stability index. The minimising of real power losses is utilised as a criterion for determining the best location and size of DG in this work. The results validate the effectiveness of DG integration, as well as the influence of various load models on the distribution system considering load growth. The proposed algorithm is tested on IEEE 33 bus distribution system and results shows that TSA outperforms existing optimization algorithms published in recent state-of-art literature.
SHINYOUNG GEUN,Henri Meijering,Fred H. van Heuveln,Jaap Wieling,Jason Halladay,Srikumar Sahasranaman,Cornelis E.C.A. Hop 대한임상약리학회 2015 Translational and Clinical Pharmacology Vol.23 No.2
The development and validation of a method for the determination of concentrations of thiocyanatein human plasma are described here. A modified colorimetric method of Bowler was used withthe following alteration in Monica Manual, Part III. In order to obtain the same sensitivity in lowamounts of clinical samples, quartz SUPRASIL® micro cuvettes have been used. The quantitationrange was between 25–500 μM. Accuracy and precision of the quality control samples, linearity ofthe calibration curve, dilution, spike recovery and stability under various conditions were evaluatedin the validation of the method and all demonstrated acceptable results. All validation results metgood laboratory practice acceptance and FDA requirements to be acceptable for application in clinicaltrials. The validated method has been used for a Phase I clinical study in cancer patients orallyadministered with either 60 mg or 80 mg of GDC-0425 containing a cyanide (CN-) group. The thiocyanatelevels from patients before and after drug administration showed no clinically significantdifferences