http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
SHINYOUNG GEUN,Henri Meijering,Fred H. van Heuveln,Jaap Wieling,Jason Halladay,Srikumar Sahasranaman,Cornelis E.C.A. Hop 대한임상약리학회 2015 Translational and Clinical Pharmacology Vol.23 No.2
The development and validation of a method for the determination of concentrations of thiocyanatein human plasma are described here. A modified colorimetric method of Bowler was used withthe following alteration in Monica Manual, Part III. In order to obtain the same sensitivity in lowamounts of clinical samples, quartz SUPRASIL® micro cuvettes have been used. The quantitationrange was between 25–500 μM. Accuracy and precision of the quality control samples, linearity ofthe calibration curve, dilution, spike recovery and stability under various conditions were evaluatedin the validation of the method and all demonstrated acceptable results. All validation results metgood laboratory practice acceptance and FDA requirements to be acceptable for application in clinicaltrials. The validated method has been used for a Phase I clinical study in cancer patients orallyadministered with either 60 mg or 80 mg of GDC-0425 containing a cyanide (CN-) group. The thiocyanatelevels from patients before and after drug administration showed no clinically significantdifferences
SHINYOUNG GEUN,Teresa Dong,Bilin Chou,Kapil Menghrajani 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.11
Recently matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) imaging has been used to analyze small molecule pharmaceutical compounds directly on tissue sections to determine spatial distribution within target tissue and organs. The data presented to date usually indicate relative amounts of drug within the tissue. The determination of absolute amounts is still done using tissue homogenization followed by traditional liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this study, the quantitative determination of loperamide, an antidiarrheal agent and a P-glycoprotein substrate, in mdr1a/1b (-/-) mouse brain tissue sections using MALDI MS on a quadrupole time-of-flight mass spectrometry is described. 5 mg/mL α-cyano-4-hydroxycinnamic acid in 50% acetonitrile with 0.1% trifluoroacetic acid and 0.5 μM reserpine was used as the MALDI matrix. The calibration curve constructed by the peak intensities of standard samples from MALDI MS was linear from 0.025 to 0.5 μM with r2 = 0.9989. The accuracy of calibration curve standards was 78.3-105.9% and the percent deviation was less than 25%. Comparison between direct MALDI tissue analysis and conventional tissue analysis using homogenization followed by electrospray LC-MS/MS was also explored.
박민호,나숙희,이희주,신병희,안병준,SHINYOUNG GEUN 한국분석과학회 2015 분석과학 Vol.28 No.2
Rosiglitazone metabolites in rat plasma were analyzed after intraperitoneal and oral administration to rats. Seven metabolites (M1-M7) were detected in rat plasma (IP and PO), and the structures were confirmed using liquid chromatography-triple time of flight (TOF) mass spectrometry; as a result, the most abundant metabolite was M5, a de-methylated rosiglitazone. Other minor in vivo metabolites were driven from monooxygenation and demethylation (M2), thiazolidinedione ring-opening (M1, M3), mono-oxygenation (M4, M7), and mono-oxygenation followed by sulfation (M6). Among them, M1 was found to be a 3-{p-[2-(N-methyl- N-2-pyridylamino)ethoxy]phenyl}-2-(methylsulfinyl)propionamide, which is a novel metabolite of rosiglitazone. There was no significant difference in the metabolic profiles resulting from the two administrations. The findings of this study provide the first comparison of circulating metabolite profiles of rosiglitazone in rat after IP and PO administration and a novel metabolite of rosiglitazone in rat plasma.