Approaches to Content-Based Academic Writing

Soraya Khonsari 아시아영어교육학회 2005 The Journal of Asia TEFL Vol.2 No.2

Minichromosome maintenance 7 protein is a reliable biological marker for human cervical progressive disease

Soraya Lobato,Alexandre Tafuri,Paula Ávila Fernandes,Marcelo Vidigal Caliari,Marcos Xavier Silva,Marcelo Antônio Pascoal Xavier,Annamaria Ravara Vago 대한부인종양학회 2012 Journal of Gynecologic Oncology Vol.23 No.1

Objective: This study focused on comparing the expression levels of p16, Ki-67, and minichromosome maintenance 7 (MCM7) protein in normal and affected cervical epithelium to ascertain the biological significance of these markers in detecting progressive cervical disease. Methods: A quantitative and based on-scanning-microscopy analysis of the three markers expression was performed in normal and cervical intraepithelial neoplasia (CIN) I, II, and III tissues. p16 area as well as p16, Ki-67, and MCM7 positive cells or nuclei were evaluated according to their distribution and extent through the cervical epithelium. Results: A clear p16 over-expression was observed in all the dysplastic epithelium tissue samples. The quantitative analysis of p16 area as well as the number of p16 positive cells was able to better discriminate the CIN lesions grades than the usual semi-quantitative analysis. The average Ki-67 labeling indexes for the normal epithelium, CIN I, CIN II, and CIN III groups were 19.8%, 27.3%, 32.8%, and 37.1%, respectively, whereas the mean MCM7 labeling indexes for the correspondent grades were 27.0%, 30.4%, 50.5%, and 67.2%. The Ki-67 and MCM7 labeling indexes were closely correlated with the CIN histological grade, with higher labeling indexe values obtained from the more severe lesions (p<0.05), being the MCM7 labeling indexes the highest values in all the CIN categories (p<0.05). Conclusion: We observed a good correlation among the p16, Ki-67, and MCM7 data. In addition, MCM7 demonstrated to be a more efficient and sensitive marker to assess disease progression in the uterine cervix. Objective: This study focused on comparing the expression levels of p16, Ki-67, and minichromosome maintenance 7 (MCM7) protein in normal and affected cervical epithelium to ascertain the biological significance of these markers in detecting progressive cervical disease. Methods: A quantitative and based on-scanning-microscopy analysis of the three markers expression was performed in normal and cervical intraepithelial neoplasia (CIN) I, II, and III tissues. p16 area as well as p16, Ki-67, and MCM7 positive cells or nuclei were evaluated according to their distribution and extent through the cervical epithelium. Results: A clear p16 over-expression was observed in all the dysplastic epithelium tissue samples. The quantitative analysis of p16 area as well as the number of p16 positive cells was able to better discriminate the CIN lesions grades than the usual semi-quantitative analysis. The average Ki-67 labeling indexes for the normal epithelium, CIN I, CIN II, and CIN III groups were 19.8%, 27.3%, 32.8%, and 37.1%, respectively, whereas the mean MCM7 labeling indexes for the correspondent grades were 27.0%, 30.4%, 50.5%, and 67.2%. The Ki-67 and MCM7 labeling indexes were closely correlated with the CIN histological grade, with higher labeling indexe values obtained from the more severe lesions (p<0.05), being the MCM7 labeling indexes the highest values in all the CIN categories (p<0.05). Conclusion: We observed a good correlation among the p16, Ki-67, and MCM7 data. In addition, MCM7 demonstrated to be a more efficient and sensitive marker to assess disease progression in the uterine cervix.

Extraction and characterization of gelatin from black-bone chicken by-products

Soraya Saenmuang,Suphatta Phothiset,Chuleeporn Chumnanka 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.4

In this study, gelatins from black-bone chicken feet and skin (BCFG and BCSG) were extracted using different NaOH concentrations, and their physicochemical properties were characterized and compared to commercial bovine gelatin (BG). It was found that the yield of BCFG was higher than BCSG, however, it contained higher amount of ash. All studied gelatins were composed of two distinct a-chains, while b-chain and c-chain were not present. The BCFG and BCSG were found to have lower pH, lower hydroxyproline content and lower thermal stability, but higher gel strength as compared with the BG. The colors of BCSG and BCSG were slightly darker than BG. The NaOH concentration did not show strong influence on physicochemical properties of the extracted gelatins, however, thermal stability and gel strength of BCSG tended to decrease with increasing of NaOH concentration. These findings suggested that black-bone chicken feet and skin could be a great source for the production of gelatin.

Biology and rearing technique for the mangrove firefly, Pteroptyx valida (Coleoptera: Lampyridae) Olivier, with discussion of additional instar in female

Soraya Jaikla,Anchana Thancharoen,Nantasak Pinkaew 국립중앙과학관 2020 Journal of Asia-Pacific Biodiversity Vol.13 No.3

With increasing pressure from many threats including firefly tourism and urbanization, rearing ofPteroptyx fireflies and reintroducing the species to habitats with low populations is a conservationchallenge. Here we report a novel rearing technique for P. valida Olivier, 1909 to develop on an artificialsubstrate, sodium polyacrylate, together with biological observations on the egg and pupa, stages whichare typically difficult to observe. The life span of P. valida under the technique was 152.7 5.2 days anddid not differ between sexes. Mated females laid 202.9 17.0 eggs per female with 84% hatching rate. The highest larval mortality rate occurred in the first and second instars with 32% and 25%, respectively. The larvae needed four to six instars to develop to adults. The developmental time of the first to sixthlarval instars ranged from 22 32 days, with the pupal stage lasting 9 days. Larvae with additional instarswere observed in lab culture, and these most often became female adults, resulting in female-biasedsexual size dimorphism as discussed in this study.

Distribution, abundance, and habitat characteristics of the congregating firefly, Pteroptyx Olivier (Coleoptera: Lampyridae) in Thailand

Soraya Jaikla,Sara M. Lewis,Anchana Thancharoen,Nantasak Pinkaew 국립중앙과학관 2020 Journal of Asia-Pacific Biodiversity Vol.13 No.3

Pteroptyx fireflies represent an important economic insect for ecotourism. Two methods, visual countingand sweep net collection, were used to survey Pteroptyx species at 23 study sites along the coast ofThailand. P. malaccae and P. valida were the most common species across all sites with significant differencesin abundance among study sites. In contrast, P. asymmetria was found only in the southernregion, and P. tener was found only at one site in Surat Thani province. Pteroptyx fireflies displayed intrees whose canopies were opened to view as opposed to those blocked by other vegetation. Firefliesmost often perched in the top section. Pteroptyx horizontal distribution was affected by ambient winddirection and speed. In addition, most display trees were located at prominent spots on either the insidebends or the outside bends of mangrove rivers. The principal component regression analysis was performedto determine which environmental factors might influence Pteroptyx species abundances andrevealed that three Pteroptyx species appear to be influenced by different combinations of environmentalvariables.

Study of Non-interactive Verifiable Secret Sharing

Anneke Soraya Hidayat,Gil-Je Lee(이길제),Young-Ju Kim(김영주),Eun-Jun Yoon(윤은준),Kee-Young Yoo(유기영) 대한전자공학회 2015 대한전자공학회 학술대회 Vol.2015 No.6

Community-Based Cross-Sectional Study of Carcinogenic Human Liver Fluke in Elderly from Surin Province, Thailand

Kaewpitoon, Soraya J.,Rujirakul, Ratana,Ueng-Arporn, Naporn,Matrakool, Likit,Namwichaisirikul, Niwatchai,Churproong, Seekaow,Wongkaewpothong, Patcharaporn,Nimkuntod, Porntip,Sripa, Banchob,Kaewpitoon, Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Background: Opisthorchis viverrini infection is a serious public-health problem in Southeast Asia. It is associated with a number of hepatobiliary diseases and the evidence strongly indicates that liver fluke infection is the etiology of cholangiocarcinoma. Objectives: This study aimed to determine Opisthorchis viverrini infection in elderly people in Surin province, Northeastern Thailand. Methods: A community-based cross-sectional survey was conducted among 333 elderly in 17 districts of Surin province, during one year period from January to December 2011. O. viverrini infection was determined using Kato's Thick Smear technique and socio-demographic were collected using predesigned semi-structured questionnaires, respectively. Results: A total of 333 elderly including 116 males and 217 females were selected from different study sites. Overall intestinal parasitic infection was 16.2%, predominantly in O. viverrini (9.91%) and followed by Strongyloides stercolaris (4.80%) and hookworm (1.50%), respectively. The O. viverrini infection was found higher in males (13.8%) than females (7.83%), and frequently in elderly 60-70 year old with 14.2%. Chi-square testing indicated that education and occupation were significantly associated with O. viverrini infection (P value = 0.02). The distribution of O. viverrini infection was found in 11 districts which was covered 64.7% of the studies areas. The highest prevalence was found in Thatum with 39.1%, and followed by Sangkha (24.0%), Buachet (21.1%), Samrong Thap (19.1%), Si Narong (15.0%), and Ratanaburi (13.3%) districts. Conclusion: This findings stress that O viverrini is still a problem in Thailand. We confirmed, for the first time, the high endemicity of human O. viverrini infections in elderly in Surin province of Thailand, underlying the fact that mass treatment and health education are urgently required.

Overweight Relation to Liver Fluke Infection among Rural Participants from 4 Districts of Nakhon Ratchasima Province, Thailand

Kaewpitoon, Soraya J,Rujirakul, Ratana,Wakkuwattapong, Parichart,Matrakool, Likit,Tongtawee, Taweesak,Panpimanmas, Sukij,Kujapun, Jirawoot,Norkaew, Jun,Photipim, Mali,Ponphimai, Sukanya,Chavengkun, Wa Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.5

A cross-sectional survey was conducted among 730 participants from 4 rural districts of Nakhon Ratchasima province, Thailand, with a reported high incidence of liver fluke infection. This study was aimed to examine and evaluate the nutritional status in relation to Opisthorchis viverrini infection. Participants were purposive selected from Chum Phuang, Mueang Yang, Bua Yai, and Kaeng Sanam Nang districts. Stool samples were prepared by Kato Katz technique and then assessed by microscopy. Anthropometry was evaluated according to the body mass index from weight and height. Descriptive statistics and Spearman rank correlation coefficients were used to evaluate the association between the nutritional status and O. viverrini infection. Of 1.64% infected with O. viverrini the highest proportions were found in age groups ${\geq}61$ and 41-50 years old, Mueang Yang district. The majorities of participants had normal weight (32.2%), followed by class II obesity (28.1 %), class I obesity (21.8%), underweight (10.3%), and class III obesity (8.63%). Nutritional status with class II obesity (rS=0.639, p<0.01) and class I obesity (rS=0.582, p<0.05), had moderately statistical significant correlations with O. viverrini infection. Meanwhile, normal weight (rS=0.437, p<0.05) and class III obesity (rS=0.384, p<0.05) demonstrated lower statistical significance. These findings raise the possibility that infection with O. viverrini may contribute to fat deposition and thereby have long-term consequences on human health. Further studies are needed to better understand whether O. viverrini contributes directly to fat deposition and possible mechanisms.

Spatial Distribution of the Population at Risk of Cholangiocarcinoma in Chum Phaung District, Nakhon Ratchasima Province of Thailand

Kaewpitoon, Soraya J,Rujirakul, Ratana,Loyd, Ryan A,Matrakool, Likit,Sangkudloa, Amnat,Kaewthani, Sarochinee,Khemplila, Kritsakorn,Eaksanti, Thawatchai,Phatisena, Tanida,Kujapun, Jirawoot,Norkaew, Jun Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.2

Background: Cholangiocarcinoma (CCA) is a serious health problem in Thailand, particularly in northeastern and northern regions, but epidemiological studies are scarce and the spatial distribution of CCA remains to be determined. A database for the population at risk is required for monitoring, surveillance and organization of home health care. This study aim was to geo-visually display the distribution of CCA in northeast Thailand, using a geographic information system and Google Earth. Materials and Methods: A cross-sectional survey was carried out in 9 sub-districts and 133 villages in Chum Phuang district, Nakhon Ratchasima province during June and October 2015. Data on demography, and the population at risk for CCA were combined with the points of villages, sub-district boundaries, district boundaries, and points of hospitals in districts, then fed into a geographical information system. After the conversion, all of the data were imported into Google Earth for geo-visualization. Results: A total of 11,960 from 83,096 population were included in this study. Females and male were 52.5%, and 47.8%, the age group 41-50 years old 33.3%. Individual risk for CCA was identifed and classified by using the Korat CCA verbal screening test as low (92.8%), followed by high risk (6.74%), and no (0.49%), respectively. Gender ($X^2$-test=1143.63, p-value= 0.001), age group ($X^2$-test==211.36, p-value=0.0001), and sub-district ($X^2$-test=1471.858, p-value=0.0001) were significantly associated with CCA risk. Spatial distribution of the population at risk for CCA in Chum Phuang district was viewed with Google Earth. Geo-visual display followed Layer 1: District, Layer 2: Sub-district, Layer 3: Number of low risk in village, Layer 4: Number of high risk in village, and Layer 5: Hospital in Chum Phuang District and their related catchment areas. Conclusions: We present the first risk geo-visual display of CCA in this rural community, which is important for spatial targeting of control efforts. Risk appears to be strongly associated with gender, age group, and sub-district. Therefor, spatial distribution is suitable for the use in the further monitoring, surveillance, and home health care for CCA.

Distribution of the Population at Risk of Cholangiocarcinoma in Bua Yai District, Nakhon Ratchasima of Thailand Using Google Map

Kaewpitoon, Soraya J,Rujirakul, Ratana,Sangkudloa, Amnat,Kaewthani, Sarochinee,Khemplila, Kritsakorn,Cherdjirapong, Karuna,Kujapun, Jirawoot,Norkaew, Jun,Chavengkun, Wasugree,Ponphimai, Sukanya,Polsri Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Background: Cholangiocarcinoma (CCA), a major problem of health in Thailand, particularly in Northeastern and Northern regions, is generally incurable and rapidly lethal because of presentation in stage 3 or 4. Early diagnosis of stage 1 and 2 could allow better survival. Therefore, this study aimed to provide a distribution map of populations at risk for CCA in BuaYai district of Nakhon Ratchasima province, Northeast Thailand. Materials and Methods: A cross-sectional survey was carried out in 10 sub-districts and 122 villages, during June and November 2015. The populations at risk for CCA were screened using the Korat CCA verbal screening test (KCVST) and then risk areas were displayed by using Google map (GM). Results: A total of 11,435 individuals from a 26,198 population completed the KCVST. The majority had a low score of risk for CCA (1-4 points; 93.3%). High scores with 6, 7 and 8 points accounted for 1.20%, 0.13% and 0.02%. The population at risk was found frequently in sub-district municipalities, followed by sub-district administrative organization and town municipalities, (F=396.220, P-value=0.000). Distribution mapping comprised 11 layers: 1, district; 2, local administrative organization; 3, hospital; 4, KCVST opisthorchiasis; 5, KCVST praziquantel used; 6, KCVST cholelithiasis; 7, KCVST raw fish consumption; 8, KCVST alcohol consumption; 9, KCVST pesticide used; 10, KCVST relative family with CCA; and 11, KCVST naive northeastern people. Geovisual display is now available online. Conclusions: This study indicated that the population at high risk of CCA in Bua Yai district is low, therefore setting a zero model project is possible. Key success factors for disease prevention and control need further study. GM production is suitable for further CCA surveillance and monitoring of the population with a high risk score in this area.