Perioperative morbidity of radical trachelectomy with lymphadenectomy in early-stage cervical cancer: a French prospective multicentric cohort

Vincent Balaya,Fabrice Lécuru,Laurent Magaud,Charlotte Ngô,Cyrille Huchon,Anne-Sophie Bats,Patrice Mathevet 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.3

Objective: The aim of this study was to determine the predictive factors of postoperative morbidity of patients who have undergone a radical trachelectomy (RT) for early-stage cervical cancer and to assess the oncologic outcomes. Methods: We retrospectively analysed the data of 2 prospective trials on sentinel node biopsy for cervical cancer (SENTICOL I and II). Patients having a RT for early-stage cervical cancer with negative sentinel lymph node and safe margins, were included. Results: Forty-nine patients met the inclusion criteria. Forty-five patients had a laparoscopicassisted vaginal technique and 4 patients a total laparoscopic technique. The median age was 32 years (range, 22–46 years). 83.7% of patients had a stage IB1 disease. There were 63.3% squamous cell carcinomas and 34.7% adenocarcinomas. The median follow-up was 46 months (range, 1–85 months). Two patients (3.3%) had a severe postoperative complication (Clavien-Dindo ≥III and/or CTCAE ≥3). The main postoperative complications were urinary (28.6%), lymphovascular (26.5%) and neurologic (14.3%). On a multivariate analysis, postoperative complications were significantly associated with history of pelvic surgery and IB1 International Federation of Gynecology and Obstetrics stage. Inclusion in high surgical skills centers decrease the risk of postoperative complications. During the follow-up, 3 patients (6.1%) had a local recurrence and one patient died from a breast cancer. Between group with complications and group without any complications, overall survival and recurrence-free survival did not significantly differ at 5-year of follow-up. Conclusion: RT has few severe postoperative complications and appears as a safe alternative to spare fertility of young patients. To guarantee best surgical outcomes, patients should be referred to expert center.

Trade in a small-range songbird, the Javan crocias, gives insight into the Asian Songbird Crisis

Vincent Nijman,Ahmad Ardiansyah,Rifqi Hendrik,Abdullah Langgeng,Sophie Manson,Katey Hedger,Muhammad Ali Imron,K. Anne-Isola Nekaris 국립중앙과학관 2021 Journal of Asia-Pacific Biodiversity Vol.14 No.2

The Asian Songbird Crisisdthe recognition that the cage bird trade is the number one threat to manysongbirdsdhas focussed on either individual bird markets or specific taxa. We here show, throughsustained monitoring, that even species that were not thought to be traded are also at risk from overexploitation. The Javan crocias Laniellus albonotatus is endemic to the montane forests of western Javaethis remoteness was thought to be the reason why it had escaped from being at risk. We surveyed21 bird markets over a 44-month period; the number of crocias recorded in markets (1.29 birds/survey)was negatively related to the distance to its habitat. The size of the bird market had no effect on thenumber of crocias we recorded. Prices (US$26.70/bird), when corrected for inflation, did not change over12 years suggesting supply can keep up with demand. Turnover is high (50% sold after 8 days), and weestimate the total trade at 1,200 to 1,500 birds/year. Legal protection of Javan crocias seen in isolationthus far is proven to be ineffective. A more inclusive approach where government bodies, conservationagencies and society participate, is needed to curb the trade in this and other imperiled songbirds.

Single-particle analysis of industrial emissions brings new insights for health risk assessment of PM

Dappe, Vincent,Uzu, Gaë,lle,Schreck, Eva,Wu, Li,Li, Xue,Dumat, Camille,Moreau, Myriam,Hanoune, Benjamin,Ro, Chul-Un,Sobanska, Sophie Elsevier 2018 Atmospheric pollution research Vol.9 No.4

Enhancement of Peripheral Nerve Regrowth by the Purine Nucleoside Analog and Cell Cycle Inhibitor, Roscovitine

Law, Vincent,Dong, Sophie,Rosales, Jesusa L.,Jeong, Myung-Yung,Zochodne, Douglas,Lee, Ki-Young Frontiers Media S.A. 2016 Frontiers in cellular neuroscience Vol.10 No.-

<P>Peripheral nerve regeneration is a slow process that can be associated with limited outcomes and thus a search for novel and effective therapy for peripheral nerve injury and disease is crucial. Here, we found that roscovitine, a synthetic purine nucleoside analog, enhances neurite outgrowth in neuronal-like PC12 cells. Furthermore, <I>ex vivo</I> analysis of pre-injured adult rat dorsal root ganglion (DRG) neurons showed that roscovitine enhances neurite regrowth in these cells. Likewise, <I>in vivo</I> transected sciatic nerves in rats locally perfused with roscovitine had augmented repopulation of new myelinated axons beyond the transection zone. By mass spectrometry, we found that roscovitine interacts with tubulin and actin. It interacts directly with tubulin and causes a dose-dependent induction of tubulin polymerization as well as enhances Guanosine-5′-triphosphate (GTP)-dependent tubulin polymerization. Conversely, roscovitine interacts indirectly with actin and counteracts the inhibitory effect of cyclin-dependent kinases 5 (Cdk5) on Actin-Related Proteins 2/3 (Arp2/3)-dependent actin polymerization, and thus, causes actin polymerization. Moreover, in the presence of neurotrophic factors such as nerve growth factor (NGF), roscovitine-enhanced neurite outgrowth is mediated by increased activation of the extracellular signal-regulated kinases 1/2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK) pathways. Since microtubule and F-actin dynamics are critical for axonal regrowth, the ability of roscovitine to activate the ERK1/2 and p38 MAPK pathways and support polymerization of tubulin and actin indicate a major role for this purine nucleoside analog in the promotion of axonal regeneration. Together, our findings demonstrate a therapeutic potential for the purine nucleoside analog, roscovitine, in peripheral nerve injury.</P>

A Fermented Soy Permeate Improves the Skeletal Muscle Glucose Level Without Restoring the Glycogen Content in Streptozotocin-Induced Diabetic Rats

Ludivine Malarde,Sophie Vincent,Luz Lefeuvre-Orfila,The´o Efstathiou,Carole Groussard,Arlette Gratas-Delamarche 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.2

Exercise is essential into the therapeutic management of diabetic patients, but their level of exercise tolerance is lowered due to alterations of glucose metabolism. As soy isoflavones have been shown to improve glucose metabolism, this study aimed to assess the effects of a dietary supplement containing soy isoflavones and alpha-galactooligosaccharides on muscular glucose, glycogen synthase (GSase), and glycogen content in a type 1 diabetic animal model. The dietary supplement tested was a patented compound, Fermented Soy Permeate (FSP), developed by the French Company Sojasun Technologies. Forty male Wistar rats were randomly assigned to control or diabetic groups (streptozotocin, 45 mg/kg). Each group was then divided into placebo or FSP-supplemented groups. Both groups received by oral gavage, respectively, water or diluted FSP (0.1 g/day), daily for a period of 3 weeks. At the end of the protocol, glycemia was noticed after a 24-h fasting period. Glucose, total GSase, and the glycogen content were determined in the skeletal muscle (gastrocnemius). Diabetic animals showed a higher blood glucose concentration, but a lower glucose and glycogen muscle content than controls. Three weeks of FSP consumption allowed to restore the muscle glucose concentration, but failed to reduce glycemia and to normalize the glycogen content in diabetic rats. Furthermore, the glycogen content was increased in FSP-supplemented controls compared to placebo controls. Our results demonstrated that diabetic rats exhibited a depleted muscle glycogen content (−25%). FSP-supplementation normalized the muscle glucose level without restoring the glycogen content in diabetic rats. However, it succeeded to increase it in the control group (+20%).

Fermented Soy Permeate Reduces Cytokine Level and Oxidative Stress in Streptozotocin-Induced Diabetic Rats

Ludivine Malarde,Carole Groussard,Luz Lefeuvre-Orfila,Sophie Vincent,The´o Efstathiou,Arlette Gratas-Delamarche 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.1

Oxidative stress and inflammation are involved in the development of type 1 diabetes and its complications. Because two compounds found in soy, that is, isoflavones and alpha-galactooligosaccharides, have been shown to exert antioxidant and anti-inflammatory effects, this study aimed to assess the effects of a dietary supplement containing these two active compounds, the fermented soy permeate (FSP). We hypothesized that FSP would be able to reduce in vivo oxidative stress and inflammation in streptozotocin (STZ)–induced type 1 diabetic rats. Thirty male Wistar rats were divided into the control placebo, diabetic placebo, and diabetic FSP-supplemented groups. They received daily, by oral gavage, water (placebo groups) or diluted FSP (0.1 g/day; FSP-supplemented group). After 3 weeks, glycemic regulation (glycemia and fructosamine level); the plasma level of carboxymethyllysine (CML), a marker of systemic oxidative stress in diabetes; and the plasma levels of inflammatory markers (CRP, IL-1β, IL-6, and uric acid) were evaluated. Markers of oxidative damage (isoprostanes and GSH/GSSG), antioxidant enzymatic activity (SOD and GPX), and Mn-SOD content were determined in skeletal muscle (gastrocnemius). Diabetic placebo rats exhibited higher CML levels, lower SOD and GPX activities, and decreased Mn-SOD contents. FSP supplementation in diabetic animals normalized the CML and antioxidant enzymatic activity levels and tended to increase Mn-SOD expression. The markers of inflammation whose levels were increased in the diabetic placebo group were markedly decreased by FSP (IL-1β: - 75%, IL-6: - 46%, and uric acid: - 17%), except for CRP. Our results demonstrate that FSP exhibited antioxidant and anti-inflammatory properties in vivo in STZ-induced diabetic rats.