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RISS 인기검색어
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- 저자 : Sooyoung Moon(문수영) (검색결과 4 건)
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문수영,최성찬,오영숙,Moon, Sooyoung,Huang, Xinxin,Choi, Sung-Chan,Oh, Young-Sook The Microbiological Society of Korea 2014 미생물학회지 Vol.50 No.2
Acyl homoserine lactone (AHL) lactonase has been proved to be the AHL-degrading enzyme with the highest substrate specificity for AHL molecules and has shown a considerable potential as low-cost and efficient quorum quenching (QQ) technique. However, few studies focused on its inhibitory effect on biofilm formation which is also a quorum sensing (QS)-regulated phenomenon. In this study, QQ activity of six isolates from biofouled reverse osmosis membranes was studied using Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NTL4 as biosensors under various conditions. All of the isolates belonged to the genus Bacillus and showed QQ activity regardless of the acyl chain length or substitution of AHL molecule. The isolates were capable of significantly inhibiting biofilm formation (46.7-58.3%) by Pseudomonas aeruginosa PAO1 and produced heat-sensitive extracellular QQ substances. The LC-MS analysis of the QQ activity of a selected isolate, RO1S-5, revealed the degradation of N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12 AHL) and the production of corresponding acyl homoserine (3-oxo-C12-HS), which indicated the activity of AHL lactonase. The broad AHL substrate range and high substrate specificity suggested that the isolate would be useful for the control of biofilm-related pathogenesis and biofouling in industrial processes. Acyl homoserine lactone (AHL) 분해효소인 lactonase는 높은 기질 특이성을 지니기 때문에 경제적이고 효율적인 쿼럼 저해 기술로 이용될 가능성을 지니고 있다. 본 연구에서는 Chromobacterium violaceum CV026과 Agrobacterium tumefaciens NTL4를 바이오센서로 이용하여 biofouling이 일어난 역삼투막 시료로부터 쿼럼 센싱과 관련된 생물막 형성을 억제하는 6종의 균주를 분리 연구하였다. 분리된 균주는 모두 Bacillus 속으로 동정되었으며, AHL 분자의 acyl 사슬 길이나 치환 종류에 상관 없이 쿼럼 저해활성을 보여주었다. 균주들은 Pseudomonas aeruginosa PAO1에 의한 생물막 형성을 46.7-58.3% 정도 감소시켰으며 이 때 저해물질은 열처리에 민감한 특성을 보여주었다. 분리 균주 중 RO1S-5를 이용하여 N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12 AHL)과 반응시킨 결과, 상응하는 acyl homoserine (3-oxo-C12-HS)이 생성되는 것을 LC-MS로 확인하여 쿼럼 저해가 lactonase 활성에 의한 것임을 규명하였다. AHL 물질에 대한 높은 특이성 등을 감안할 때 분리 균주 RO1S-5는 생물막 형성과 관련된 질병이나 산업공정 중 발생하는 biofouling을 해결하는데 유용하게 쓰일 수 있을 것으로 기대된다.
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CVJ 절각력 저감을 위한 설계인자 민감도 분석 및 최적화 연구
김기헌(Kihun Kim),문수영(Sooyoung Moon),이재원(Jaewon Lee) 한국자동차공학회 2019 한국자동차공학회 학술대회 및 전시회 Vol.2019 No.11
Due to the high performance of the constant velocity joint, various problems may arise in terms of the function and strength of the constant velocity joint. Due to the miniaturization, strength degradation may occur. High keratosis of the joints can lead to problems of functional problem strength degradation. In this study, we discuss the articulation (functional) problems of constant velocity joints that can be caused by high keratinization. We have applied a design concept called angle offset to implement a high-angle joint. The angle offset can compensate for the strength by ensuring sufficient groove depth even under high angled conditions. However, there is a disadvantage in that machining is difficult and it is not easy to match the exact dimensions. As a result, a high friction force between the initial ball and the groove is generated, thereby causing a high bending resistance. High friction forces reduce the efficiency of the joint and can be a source of durability problems. In this study, the design factors affecting the bending resistance are derived and the sensitivity is analyzed to derive the factors that can minimize the bending resistance. The optimization technique uses DOE and the strength analysis will also confirm the strength robustness of the model.
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