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김찬식,강순선,오명협,현해남 濟州大學校亞熱帶農業硏究所 1996 亞熱帶農業硏究 Vol.13 No.-
SummaryDistributions of actinomycetes, bacteria, and fungi were examined in citrus house and field soils sampled from volcanic ash soil area. pH, electrical conductivity, and exchangeable K, Ca, and Mg content in citrus house soil were higher than them in citrus field soil, however, total nitrogen content in citrus house soil is lower than that in citrus field soil.Number of total microorganisms in citrus house soil was about 3.1×105, which was 10 times lower than that in citrus field soil, and also that was remarkably lower than similar group of volcanic ash soil in cultivating and upland soil. Number of actinomycetes and bacteria in citrus house soil were similar to citrus field soil, however, that of fungi in citrus house soil was lower than that in citrus field soil.Electical conductivity and Bray No. 1 P in citrus field soil and pH and exchangeable Mg content in citrus house soil were positively correlated with distribution of actinomycetes in soils and Bray No. 1 P in citrus field soil and exchangeable Mg content in citrus house soil were negatively correlated with distribution of fungi in soils Keywords : volcanic ash citrus orchard soil, soil chemical property, actinomycetes, bacteria, fungi in soils
Equine hyperimmune serum protects mice against Clostridium difficile spore challenge
Weiwei Yan,Kang-Soon Shin,Shih-Jon Wang,Hua Xiang,Thomas Divers,Sean McDonough,James Bowman,Anne Rowlands,Bruce Akey,Hussni Mohamed,Yung-Fu Chang 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2
Clostridium (C.) difficile is a common cause of nosocomialdiarrhea in horses. Vancomycin and metronidazole havebeen used as standard treatments but are only moderatelyeffective, which highlights the need for a novel alternativetherapy. In the current study, we prepared antiserum ofequine origin against both C. difficile toxins A and B as wellas whole-cell bacteria. The toxin-neutralizing activities of theantibodies were evaluated in vitro and the prophylacticeffects of in vivo passive immunotherapy were demonstratedusing a conventional mouse model. The data demonstratedthat immunized horses generated antibodies against bothtoxins A and B that possessed toxin-neutralizing activity. Additionally, mice treated with the antiserum lost less weightwithout any sign of illness and regained weight back to anormal range more rapidly compared to the control groupwhen challenged orally with 107 C. difficile spores 1 day afterserum injection. These results indicate that intravenousdelivery of hyperimmune serum can protect animals from C. difficile challenge in a dose-dependent manner. Hence,immunotherapy may be a promising prophylactic strategyfor preventing C. difficile infection in horses.
CONFORMATION AND FUNCTIONAL PROPERTIES OF MODIFIED SOYBEAN GLYCININS
Lee, Sam Pin,Yang, Kwon Min,Ko, Kyung Cheal,Kang, Soon Sean,Kim, Chan Shick,Ko, Se Kwang 제주대학교 방사능이용연구소 1997 연구보고 Vol.11 No.-
To improve the nutritional and functional properties of soybean proglycinin, cDNA coding soybean proglycinin was manipulated using the synthetic oligonucleotides. Modified expression plasmids pKGA??B?? Ⅳ+4Met was used as template for mutation. Each amino acid sequence of Gln-Leu-Ile-Phe-Lys-Leu and Gln-Leu-Phe-Asp-Gln-Thr-Pro-Arg-Val-Phe was inserted between Pro467 and Gln468 in the variable domain V. The insertion of new amino acids was confirmed by the creation of Pvu II restriction site. Expression plasmids carrying a modified cDNA were constructed and expressed in E. Coli. Modified soybean glycinins were purified by salt precipitation, ion-exchange chromatography and cryoprecipitation. The physico-chemical and functional properties of modified soybean glycinins were determined.