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Kim, Hyo-Jin,Nam, Soon-Hyeun,Kim, Hyun-Jung,Park, Hyo-Sang,Ryoo, Hyun-Mo,Kim, Shin-Yoon,Cho, Tae-Joon,Kim, Seung-Gon,Bae, Suk-Chul,Kim, In-San,Stein, Janet L.,van Wijnen, Andre J.,Stein, Gary S.,Lian, Liss 2006 Journal of Cellular Physiology Vol.207 No.1
<P>Cleidocranial dysplasia (CCD) is an autosomal dominant disorder caused by haploinsufficiency of the RUNX2 gene. In this study, we analyzed by direct sequencing RUNX2 mutations from eleven CCD patients. Four of seven mutations were novel: two nonsense mutations resulted in a translational stop at codon 50 (Q50X) and 112 (E112X); a missense mutation converted arginine to glycine at codon 131 (R131G); and an exon 1 splice donor site mutation (donor splice site GT/AT, IVS1 + 1G > A) at exon 1–intron junction resulted in the deletion of QA stretch contained in exon 1 of RUNX2. We focused on the functional analysis of the IVS1 + 1G > A mutation. A full-length cDNA of this mutation was cloned (RUNX2Δe1) and expressed in Chinese hamster ovary (CHO) and HeLa cells. Functional analysis of RUNX2Δe1 was performed with respect to protein stability, nuclear localization, DNA binding, and transactivation activity of a downstream RUNX2 target gene. Protein stability of RUNX2Δe1 is similar to wild-type RUNX2 as determined by Western blot analysis. Subcellular localization of RUNX2Δe1, assessed by in situ immunofluorescent staining, was observed with partial retention in both the nucleus and cytoplasm. This finding is in contrast to RUNX2 wild-type, which is detected exclusively in the nucleus. DNA binding activity was also compromised by the RUNX2Δe1 in gel shift assay. Finally, RUNX2Δe1 blocked transactivation of the osteocalcin gene determined by transient transfection assay. Our findings demonstrate for the first time that the CCD phenotype can be caused by a splice site mutation, which results in the deletion of N-terminus amino acids containing the QA stretch in RUNX2 that contains a previously unidentified second nuclear localization signal (NLS). We postulate that the QA sequence unique to RUNX2 contributes to a competent structure of RUNX2 that is required for nuclear localization, DNA binding, and transactivation function. J. Cell. Physiol. 207: 114–122, 2006. © 2005 Wiley-Liss, Inc.</P>
이지연,김원석,송서영,이순일,박준오,김기현,고영혜,정철원,임영혁,강원기,이홍기,이희정,박찬형,박근칠 대한내과학회 2002 대한내과학회지 Vol.63 No.1
Primary lymphoma of the urinary bladder is a rare non-epithelial bladder tumor accounting for less than 1% of all bladder tumors. Approximately 17 cases of MALT lymphomas of bladder have been reported in the literature. Most reported MALT lymphomas of bladder have a female sexual preponderance with a mean age of 58 years with common presenting symptoms of hematuria, dysuria and urinary frequency. The reported prognosis of MALT lymphoma of the urinary bladder is excellent. We report a case of MALT lymphoma of urinary bladder in a 57-year-old woman patient who presented with a two-year history of persistent dysuria and urinary frequency. An intravenous pyelogram and cystoscopy revealed a 1 cm focal elevated lesion at the base of urinary bladder. The tissue obtained by transurethral resection (TUR) showed plasma cell infiltration consistent with low grade marginal zone B cell lymphoma. The immunohistochemical studies showed an immunoglobulin restriction to lambda light chain while the nested polymerase chain reaction analysis of the tissue showed a monoclonal Ig heavy-chain gene rearrangement. The clinical staging protocol revealed that the tumor was primarily arising from the urinary bladder with no evidence of other site involvements. The patient received radiation therapy of 3060 cGy in 17 fractions.
Photo-induced hybrid nanopatterning of titanium dioxide <i>via</i> direct imprint lithography
Park, Hyeong-Ho,Choi, Dae-Geun,Zhang, Xin,Jeon, Sohee,Park, Seong-Je,Lee, Soon-Won,Kim, Sarah,Kim, Ki-don,Choi, Jun-Hyuk,Lee, Jihye,Yun, Dae Keun,Lee, Ki Joong,Park, Hyung-Ho,Hill, Ross Henry,Jeong, J Royal Society of Chemistry 2010 Journal of materials chemistry Vol.20 No.10
<P>A novel ultraviolet (UV)-assisted imprinting procedure that employs photosensitive titanium(<SMALL>IV</SMALL>) di-n-butoxide bis(2-ethylhexanoate) is presented for the fabrication of well-ordered titanium dioxide (TiO<SUB>2</SUB>) nanostructures at room temperature. The main novelty of this technique is the use of the photosensitive titanium organic compound, rather than a commonly used UV-curable resin, for direct UV-assisted nanoimprint lithography. Fourier transform infrared and X-ray photoelectron spectroscopy studies suggest that exposure to UV light resulted in the gradual removal of organic groups from films prepared from titanium(<SMALL>IV</SMALL>) di-n-butoxide bis(2-ethylhexanoate) photochemically and successively converted the films to TiO<SUB>2</SUB> at room temperature. This approach allows direct fabrication of TiO<SUB>2</SUB> nanopatterns with lines down to 35 nm in width, hole arrays of 265 nm in diameter, and three-dimensional TiO<SUB>2</SUB> hybrid micro/nano-patterns without observable defects for use in applications where ordered surface nanostructures are required, such as photovoltaics, photonics, and optical waveguides.</P> <P>Graphic Abstract</P><P>A novel UV-assisted imprinting procedure that employs photosensitive Ti(<SMALL>IV</SMALL>) di-n-butoxide bis(2-ethylhexanoate) is presented for the fabrication of well-ordered TiO<SUB>2</SUB> and 3-D TiO<SUB>2</SUB> hybrid micro/nano-patterns at room temperature. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b921343k'> </P>
Jun, Ikhyun,Park, Hyung Soon,Piao, He,Han, Jung Woo,An, Min Ji,Yun, Byeong Gyu,Zhang, Xianglan,Cha, Yong Hoon,Shin, You Keun,Yook, Jong In,Jung, Jinsei,Gee, Heon Yung,Park, Joon Seong,Yoon, Dong Sup,J Nature Publishing Group 2017 The British journal of cancer Vol. No.
<P><B>Background:</B></P><P>Anoctamin (ANO)/transmembrane member 16 (TMEM16) proteins mediate diverse physiological and pathophysiological functions including cancer cell proliferation. The present study aimed to identify the role of ANOs in pancreatic cancer.</P><P><B>Methods:</B></P><P>In an initial screen of ANOs, ANO9/TMEM16J was overexpressed in pancreatic cancer cells, and its role in the pathogenesis of pancreatic cancer was evaluated using an integrated <I>in vitro</I> and <I>in vivo</I> approach. To determine clinical relevance of the experimental findings, the prognostic value of ANO9 was evaluated in patients with pancreatic cancer.</P><P><B>Results:</B></P><P>The ANO9 mRNA and protein levels were increased in pancreatic cancer-derived cells. Exogenous expression of <I>ANO9</I> in PANC-1 cells significantly increased cell proliferation in cell cultures and in mice. In contrast, knockdown of <I>ANO9</I> in AsPC-1, BxPC-3, and Capan-2 cells strongly inhibited cell proliferation. Mechanistic analysis suggested that physical association of ANO9 with epidermal growth factor receptor (EGFR) underlies ANO9-induced cell proliferation. Knockdown <I>of ANO9</I> augmented the effects of the EGFR inhibitor and the cytotoxic agent on pancreatic cancer cell proliferation. In addition, high ANO9 expression is a poor prognostic factor in patients with pancreatic cancer.</P><P><B>Conclusions:</B></P><P>The ANO9/TMEM16J appears to be a clinically useful prognostic marker for pancreatic cancer and a potential therapeutic target.</P>
Park, Sung Wook,Choi, Kyong,Lee, Hwanghee,Park, Sung Kwang,Turner, Anthony J.,Hooper, Nigel M.,Park, Haeng Soon 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-
The release mechanism of the glycosyl-phosphatidylinositol (GPI)-anchored renal dipeptidase (EC 3.4.13.19) in vivo has been investigated. Triton X-114 phase separation indicated that the dipeptidase is exclusively present as a hydrophilic form in urine from porcine, rat, rabbit and human. Western blot analysis of human and porcine purified dipeptidase and the urine concentrates with anti-(cross-reacting determinant) serum demonstrated the presence of inositol 1, 2-cyclic monophosphate indicating that the renal dipeptidase had been released from the membrane by the action of a phospholipase C. This is the first direct evidence for cleavage of a human GPI-anchored protein by a responsible phospholipase C in vivo.
Effect of thermal annealing on low-energy C-ion irradiated MgB<sub>2</sub> thin films
Jung, Soon-Gil,Son, Seung-Ku,Pham, Duong,Lim, W.C.,Song, J.,Kang, W.N.,Park, T. The Korea Institute of Applied Superconductivity a 2019 한국초전도저온공학회논문지 Vol.21 No.3
We investigate the effect of thermal annealing on $MgB_2$ thin films with thicknesses of 400 and 800 nm, irradiated by 350 keV C-ions with a dose of $1{\times}10^{15}atoms/cm^2$. Irradiation by low-energy C-ions produces atomic lattice displacement in $MgB_2$ thin films, improving magnetic field performance of critical current density ($J_c$) while reducing the superconducting transition temperature ($T_c$). Interestingly, the lattice displacement and the $T_c$ are gradually restored to the original values with increasing thermal annealing temperature. In addition, the magnetic field dependence of $J_c$ also returns to that of the pristine state together with the restoration of $T_c$. Because $J_c$(H) is sensitive to the type and density of the disorder, i.e. vortex pinning, the recovery of $J_c$(H) in irradiated $MgB_2$ thin films by thermal annealing indicates that low-energy C-ion irradiation on $MgB_2$ thin films primarily causes lattice displacement. These results provide new insights into the application of low-energy irradiation in strategically engineering critical properties of superconductors.
Simulation study of proposed off-midplane lower hybrid current drive in KSTAR
Bae, Young-soon,Shiraiwa, S,Bonoli, P,Wallace, G,Wright, J C,Parker, R,Kim, J H,Namkung, W,Cho, M H,Park, B H,Yoon, S W,Oh, Y K,Park, H IOP 2016 Plasma physics and controlled fusion Vol.58 No.7
<P>A new proposal of lower hybrid (LH) wave launching is studied for efficient current drive aiming for high performance H-mode operation in Korea Superconducting Tokamak Advanced Research (KSTAR). This new concept is the off-midplane launch which results in a rapid up-shift of the parallel component of refractive index and hence simultaneously maintains good wave accessibility and efficient single pass absorption via Landau damping. In order to locate an optimal position of the launcher in the poloidal direction, the ray-tracing and Fokker–Planck codes were used. Based on a survey of the LH wave launch parameters and operation conditions including the compatibility issues with the existing in-vessel components, the LH wave launch from the top position near the upper <I>X</I>-point of the plasma separatrix provides the possibility to eliminate the accessibility problem and reduce parasitic edge loss for the KSTAR high performance H-mode operation scenario using 5 GHz lower hybrid current drive.</P>
박성호,이순기,고향진,김낙준 대한금속재료학회(대한금속학회) 1999 대한금속·재료학회지 Vol.37 No.6
The present study is aimed at developing the high strength, high formability low alloy steels having retained austenite as one of the constituents. The main emphasis has been put on understanding the effects of processing variables and alloying elements (Cu, Nb) on the microstructure and mechanical properties of hot-rolled Fe-C-Mn-Si base TRIP (Transformation Induced Plasticity) steels. Experimental results show that microstructure of hot-rolled alloys consists of ferrite, bainite, martensite and retained austenite, whose volume fraction and morphology vary depending on the hot-rolling conditions and alloy compositions. The increase in the quench-finish temperature results in a homogeneous distribution of fine granular particles of retained austenite and hard second phases in a ferrite matrix and accordingly a good combination of strength and ductility. The Cu-containing alloy has been found to improve mechanical properties over the base alloy due to the precipitation of fine ε-Cu in ferrite grains. Nb addition increases the dynamic recrystallization temperature of austenite, resulting in the formation of coarse pan-caked bainite packets. Based on these results, the optimum microstructure for a better combination of strength and ductility is suggested to be the one having granular particles of retained austenite and hard phase (martensite or bainite) in a fine ferrite matrix.