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Automatic Parking System using Background Subtraction with CCTV Environment
Jungseok Cho,Jinrak Park,Untae Baek,Donghyun Hwang,Seibum Choi,Soohyun Kim,Kyungsoo Kim 제어로봇시스템학회 2016 제어로봇시스템학회 국제학술대회 논문집 Vol.2016 No.10
In this paper, a new automatic parking approach that uses a surveillance camera is suggested. Many parking lots have surveillance cameras. Vehicle position, attitude, and parking space detection methods using a based on CCTV environment are introduced. An entire auto parking system is realized using a small experimental vehicle. Existing auto parking systems are primarily ADAS (advanced driver assistance systems) that use extra sensors such as external cameras, ultrasonic sensors, and so on. The authors suggest an automatic parking system that does not impose an economic burden on drivers. Instead, drivers can use an automatic parking system based on CCTV environment, a central process computer, and a communication method between the computer and the driver’s car.
우울 수준에 따른 가상멀미 경험이 가상현실 기반 신경인지과제 수행에 미치는 영향 : 고령집단을 중심으로
김인하(Kim, Inha)박정훈(Park, Junghun),이서정(Lee, Seojung),민성(Min, Sung),전주영(Jeon, Juyoung),조건우(Cho, Geonwoo),박혜연(Park, Haeyean),박다솔(Park, Dasol)박수현(Park, Soohyun) 한국재활심리학회 2021 재활심리연구 Vol.28 No.1
본 연구는 고령 집단에서 가상현실기반 평가 및 치료를 활용하는데 있어, 주요 장애물이 될 수 있는 가상멀미의 영향을 탐색하기 위해 진행되었다. 이 가운데서도 신경인지과제 수행 시, 우울감의 수준에따라 가상멀미가 과제수행에 미치는 영향을 탐색하고자 하였으며, 이를 확인하기 위하여 만 50세 이상성인 73명이 (1) 가상현실 기반 신경인지과제, (2) 가상멀미 수준과 우울을 측정하는 자기보고식 설문을 완료하였다. 연구 결과, 우울감이 클수록 가상현실 프로그램 시행에 따른 가상멀미 변화량이 커지는 경향을 보이며, 준임상 수준 이상에 해당하는 고우울 집단에서는 가상멀미가 가상현실기반 신경인지과제 수행에 유의한 영향을 미치는 것으로 나타났다. 저우울 집단에서는 이러한 영향이 유의하지 않았다. 이를 통해 준임상수준 이상의 고우울 집단에서는 지각된 가상멀미 수준이 가상현실기반 신경인지과제 수행에 부정적 영향을 미칠 수 있음이 시사되었다. 이러한 결과는 향후 고령의 우울집단에서 가상현실 치료를 활성화하기 위해, 주요 부작용인 가상멀미의 영향을 확인했다는 점에서 의의가 있으며, 이를 비롯한 본 연구의 의의와 한계점 및 후속 연구에 대해 논의하였다. This study was conducted to explore the effects of simulator sickness, which can be a major obstacle in the use of virtual reality-based evaluation and treatment in the elderly group. Also, the effect of depression level on simulator sickness and a virtual reality (VR)-based neurocognitive task performance was investigated. Seventy-three participants over the age of 50 years completed the (1) VR-based neurocognitive task and (2) self-report questionnaires measuring simulator sickness, and depressive symptoms. The results indicated that depression predicts the severity of perceived simulator sickness, and simulator sickness had a significant effect on the performance of the virtual reality-based neurocognitive task in a group with sub-clinical or clinical levels of depression. Based on the results, the implications, limitations and suggestions for future studies were discussed.
Rapid and Accurate Measurement of Ideality Factor and Parasitic Resistances of Thin Film Solar Cells
Park, Sungeun,Kim, Soo Min,Park, Se Jin,Bae, Soohyun,Park, Hyomin,Nam, Jung gyu,Lee, Dongho,Yang, Jung Yup,Kim, Dong Seop,Mo, Chanbin,Kim, Young-Su,Kim, Jihyun,Lee, Hae-Seok,Kang, Yoonmook The Electrochemical Society 2018 ECS journal of solid state science and technology Vol.7 No.6
<P>The sunshade method for the measurement of the ideality factor and resistances of solar cells is commonly used in silicon solar cells. However, the method is rarely employed for thin-film solar cells. In this paper, the sunshade method is applied to CIGS solar cells, and it is shown that the three major assumptions of the method remain valid for CIGS solar cells. The extracted parameters are compared to those calculated using other common methods, and the ideality factors and R-s values are quickly determined and found to be nearly the same as those extracted through illuminated I-V fitting and dark I-V fitting. (C) 2018 The Electrochemical Society.</P>
Characterization of Phosphoenolpyruvate Carboxylase from Oceanimonas smirnovii in Escherichia coli.
Park, Soohyun,Lee, Wangjun,Kim, Hyeonsoo,Pack, Seung Pil,Lee, Jinwon Humana Press 2015 Applied biochemistry and biotechnology Vol.177 No.1
<P>In this study, phosphoenolpyruvate carboxylase (PEPC) derived from Oceanimonas smirnovii (OS) was expressed as a soluble protein in Escherichia coli BL21(DE3). We isolated OS-PEPC (a recombinant PEPC protein) by his-tag purification. The purified protein showed a single band upon analysis with SDS-PAGE, and it had an apparent molecular mass of 98?kDa. Pufied OS-PEPC showed a specific activity value of 21.8??0.495?U/mg protein. Especially, OS-PEPC showed the enzymatic activity between 40 and 50?C. It maintained enzymatic activity in basic pH conditions (pH value, 9-10). We also measured OS-PEPC PEP and HCO3 (-) saturation kinetics and confirmed the effect of divalent cation on OS-PEPC activity.</P>
Park, Soohyun,Chang, Kwang Suk,Jin, Eonseon,Pack, Seung Pil,Lee, Jinwon Springer-Verlag 2013 Bioprocess and biosystems engineering Vol.36 No.1
<P>A new phosphoenolpyruvate carboxylase (PEPC) gene of Dunaliella salina is identified using homology analysis was conducted using PEPC gene of Chlamydomonas reinhardtii and Arabidopsis thaliana. Recombinant E. coli SGJS115 with increased production of malate and oxaloacetate was developed by introducing codon-optimized phosphoenolpyruvate carboxylase2 (OPDSPEPC2) gene of Dunaliella salina. E. coli SGJS115 yielded a 9.9 % increase in malate production. In addition, E. coli SGJS115 exhibited two times increase in the yield of oxaloacetate over the E. coli SGJS114 having identified PEPC2 gene obtained from Dunaliella salina.</P>
Park, Soohyun,Lee, Jae-ung,Cho, Sukhyeong,Kim, Hyeonsoo,Oh, Han Bin,Pack, Seung Pil,Lee, Jinwon Elsevier Science Publishers 2017 Journal of biotechnology Vol.241 No.-
<P>Carbon dioxide (CO2) is an abundant and cheap carbon source that is partly responsible for global warming in the atmosphere. The objective of this study was to construct a recombinant E. coli strain that can show enhanced production of succinate derived from CO2. In this study, we confirmed the enhancement of utilization by analyzing succinate containing one carbon-13 (C-13) derived from (CO2)-C-13. Firstly, the carbonic anhydrase gene (SP(-)HCCA) derived from Hahella chejuensis KCTC 2396 was over-expressed to enhance carbon flux toward bicarbonate ion (HCO3-) synthesis in E. coli. The phosphoenolpyruvate carboxylase gene (ppc) was over-expressed to enhance the production of oxaloacetate by enhancing the carbon flux. Compared with the control strain, the percentage of the succinate containing one C-13 (succinate(119)) to total succinate was enhanced by approximately 2.80-fold and the amount of succinate(119) also increased by approximately 4.09-fold in SGJS120. Secondly, the lactate dehydrogenase gene (IdhA) was deleted to re-direct the utilization of the carbon source from glucose to enhance succinate production in SGJS120. However, IdhA deletion did not increase CO2 utilization in SJGS120. Finally, the phosphotransferase system gene (ptsG) and pyruvate kinase F gene (pykF) were deleted to increase the amount of phosphoenolpyruvate (PEP). SGJS126 (pykF deletion strain) showed the highest increase, which was 6.05-fold higher than the control strain. From the results, SP(-)HCCA overexpression and pykF deletion may be useful for enhancing CO2 utilization in E. coli. Additionally, engineered strains showed the potential to reduce the cost of succinate production by using an industrially cheaper carbon source such as CO2 and converting CO2 to a valuable chemical. (C) 2016 Published by Elsevier B.V.</P>
Park, Soohyun,Pack, Seung Pil,Lee, Jinwon Humana Press 2012 Applied biochemistry and biotechnology Vol.167 No.7
<P>We examined the expression of the phosphoenolpyruvate carboxylase (PEPC) gene from marine bacteria in Escherichia coli using codon optimization. The codon-optimized PEPC gene was expressed in the E. coli K-12 strain W3110. SDS-PAGE analysis revealed that the codon-optimized PEPC gene was only expressed in E. coli, and measurement of enzyme activity indicated the highest PEPC activity in the E. coli SGJS112 strain that contained the codon-optimized PEPC gene. In fermentation assays, the E. coli SGJS112 produced the highest yield of oxaloacetate using glucose as the source and produced a 20-times increase in the yield of malate compared to the control. We concluded that the codon optimization enabled E. coli to express the PEPC gene derived from the Glaciecola sp. HTCC2999. Also, the expressed protein exhibited an enzymatic activity similar to that of E. coli PEPC and increased the yield of oxaloacetate and malate in an E. coli system.</P>