Quantitative and qualitative analysis of the flow field development through T99 draft tube caused by optimized inlet velocity profiles

Galvan, Sergio,Reggio, Marcelo,Guibault, Francois,Solorio, Gildardo Korean Society for Fluid machinery 2015 International journal of fluid machinery and syste Vol.8 No.4

The effect of the inlet swirling flow in a hydraulic turbine draft tube is a very complex phenomenon, which has been extensively investigated both theoretically and experimentally. In fact, the finding of the optimal flow distribution at the draft tube inlet in order to get the best performance has remained a challenge. Thus, attempting to answer this question, it was assumed that through an automatic optimization process a Genetic Algorithm would be able to manage a parameterized inlet velocity profile in order to achieve the best flow field for a particular draft tube. As a result of the optimization process, it was possible to obtain different draft-tube flow structures generated by the automatic manipulation of parameterized inlet velocity profiles. Thus, this work develops a qualitative and quantitative analysis of these new draft tube flow field structures provoked by the redesigned inlet velocity profiles. The comparisons among the different flow fields obtained clearly illustrate the importance of the flow uniformity at the end of the conduit. Another important aspect has been the elimination of the re-circulating flow area which used to promote an adverse pressure gradient in the cone, deteriorating the pressure recovery effect. Thanks to the evolutionary optimization strategy, it has been possible to demonstrate that the optimized inlet velocity profile can suppress or mitigate, at least numerically, the undesirable draft tube flow characteristics. Finally, since there is only a single swirl number for which the objective function has been minimized, the energy loss factor might be slightly affected by the flow rate if the same relation of the axial-tangential velocity components is maintained, which makes it possible to scale the inlet velocity field to different operating points.

Quantitative and qualitative analysis of the flow field development through T99 draft tube caused by optimized inlet velocity profiles.

Sergio Galván,Marcelo Reggio,François Guibault,Gildardo Solorio 한국유체기계학회 2015 International journal of fluid machinery and syste Vol.8 No.4

The effect of the inlet swirling flow in a hydraulic turbine draft tube is a very complex phenomenon, which has been extensively investigated both theoretically and experimentally. In fact, the finding of the optimal flow distribution at the draft tube inlet in order to get the best performance has remained a challenge. Thus, attempting to answer this question, it was assumed that through an automatic optimization process a Genetic Algorithm would be able to manage a parameterized inlet velocity profile in order to achieve the best flow field for a particular draft tube. As a result of the optimization process, it was possible to obtain different draft-tube flow structures generated by the automatic manipulation of parameterized inlet velocity profiles. Thus, this work develops a qualitative and quantitative analysis of these new draft tube flow field structures provoked by the redesigned inlet velocity profiles. The comparisons among the different flow fields obtained clearly illustrate the importance of the flow uniformity at the end of the conduit. Another important aspect has been the elimination of the re-circulating flow area which used to promote an adverse pressure gradient in the cone, deteriorating the pressure recovery effect. Thanks to the evolutionary optimization strategy, it has been possible to demonstrate that the optimized inlet velocity profile can suppress or mitigate, at least numerically, the undesirable draft tube flow characteristics. Finally, since there is only a single swirl number for which the objective function has been minimized, the energy loss factor might be slightly affected by the flow rate if the same relation of the axial-tangential velocity components is maintained, which makes it possible to scale the inlet velocity field to different operating points.

α-Gal Nanoparticles in CNS Trauma: I. In Vitro Activation of Microglia Towards a Pro-Healing State

Gopalakrishnan Bhavani,Galili Uri,Dunbar August,Solorio Luis,Shi Riyi,Li Jianming 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.3

Background: Macrophages and microglia play critical roles after spinal cord injury (SCI), with the pro-healing, anti-inflammatory (M2) subtype being implicated in tissue repair. We hypothesize that promoting this phenotype within the post-injured cord microenvironment may provide beneficial effects for mitigating tissue damage. As a proof of concept, we propose the use of nanoparticles incorporating the carbohydrate antigen, galactose-α-1,3-galactose (α-gal epitope) as an immunomodulator to transition human microglia (HMC3) cells toward a pro-healing state. Methods: Quiescent HMC3 cells were acutely exposed to α-gal nanoparticles in the presence of human serum and subsequently characterized for changes in cell shape, expression of anti or pro-inflammatory markers, and secretion of phenotype-specific cytokines. Results: HMC3 cells treated with serum activated α-gal nanoparticles exhibited rapid enlargement and shape change in addition to expressing CD68. Moreover, these activated cells showed increased expression of anti-inflammatory markers like Arginase-1 and CD206 without increasing production of pro-inflammatory cytokines TNF-α or IL-6. Conclusion: This study is the first to show that resting human microglia exposed to a complex of α-gal nanoparticles and anti-Gal (from human serum) can be activated and polarized toward a putative M2 state. The data suggests that α-gal nanoparticles may have therapeutic relevance to the CNS microenvironment, in both recruiting and polarizing macrophages/microglia at the application site. The immunomodulatory activity of these α-gal nanoparticles post-SCI is further described in the companion work (Part II). Background: Macrophages and microglia play critical roles after spinal cord injury (SCI), with the pro-healing, anti-inflammatory (M2) subtype being implicated in tissue repair. We hypothesize that promoting this phenotype within the post-injured cord microenvironment may provide beneficial effects for mitigating tissue damage. As a proof of concept, we propose the use of nanoparticles incorporating the carbohydrate antigen, galactose-α-1,3-galactose (α-gal epitope) as an immunomodulator to transition human microglia (HMC3) cells toward a pro-healing state. Methods: Quiescent HMC3 cells were acutely exposed to α-gal nanoparticles in the presence of human serum and subsequently characterized for changes in cell shape, expression of anti or pro-inflammatory markers, and secretion of phenotype-specific cytokines. Results: HMC3 cells treated with serum activated α-gal nanoparticles exhibited rapid enlargement and shape change in addition to expressing CD68. Moreover, these activated cells showed increased expression of anti-inflammatory markers like Arginase-1 and CD206 without increasing production of pro-inflammatory cytokines TNF-α or IL-6. Conclusion: This study is the first to show that resting human microglia exposed to a complex of α-gal nanoparticles and anti-Gal (from human serum) can be activated and polarized toward a putative M2 state. The data suggests that α-gal nanoparticles may have therapeutic relevance to the CNS microenvironment, in both recruiting and polarizing macrophages/microglia at the application site. The immunomodulatory activity of these α-gal nanoparticles post-SCI is further described in the companion work (Part II).

α-Gal Nanoparticles in CNS Trauma: II. Immunomodulation Following Spinal Cord Injury (SCI) Improves Functional Outcomes

Gopalakrishnan Bhavani,Galili Uri,Saenger Megan,Burket Noah J.,Koss Wendy,Lokender Manjari S.,Wolfe Kaitlyn M.,Husak Samantha J.,Stark Collin J.,Solorio Luis,Cox Abigail,Dunbar August,Shi Riyi,Li Jian 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.3

BACKGROUND: Previous investigations have shown that local application of nanoparticles presenting the carbohydrate moiety galactose-α-1,3-galactose (α-gal epitopes) enhance wound healing by activating the complement system and recruiting pro-healing macrophages to the injury site. Our companion in vitro paper suggest α-gal epitopes can similarly recruit and polarize human microglia toward a pro-healing phenotype. In this continuation study, we investigate the in vivo implications of α-gal nanoparticle administration directly to the injured spinal cord. METHODS: α-Gal knock-out (KO) mice subjected to spinal cord crush were injected either with saline (control) or with α-gal nanoparticles immediately following injury. Animals were assessed longitudinally with neurobehavioral and histological endpoints. RESULTS: Mice injected with α-gal nanoparticles showed increased recruitment of anti-inflammatory macrophages to the injection site in conjunction with increased production of anti-inflammatory markers and a reduction in apoptosis. Further, the treated group showed increased axonal infiltration into the lesion, a reduction in reactive astrocyte populations and increased angiogenesis. These results translated into improved sensorimotor metrics versus the control group. CONCLUSIONS: Application of α-gal nanoparticles after spinal cord injury (SCI) induces a pro-healing inflammatory response resulting in neuroprotection, improved axonal ingrowth into the lesion and enhanced sensorimotor recovery. The data shows α-gal nanoparticles may be a promising avenue for further study in CNS trauma. BACKGROUND: Previous investigations have shown that local application of nanoparticles presenting the carbohydrate moiety galactose-α-1,3-galactose (α-gal epitopes) enhance wound healing by activating the complement system and recruiting pro-healing macrophages to the injury site. Our companion in vitro paper suggest α-gal epitopes can similarly recruit and polarize human microglia toward a pro-healing phenotype. In this continuation study, we investigate the in vivo implications of α-gal nanoparticle administration directly to the injured spinal cord. METHODS: α-Gal knock-out (KO) mice subjected to spinal cord crush were injected either with saline (control) or with α-gal nanoparticles immediately following injury. Animals were assessed longitudinally with neurobehavioral and histological endpoints. RESULTS: Mice injected with α-gal nanoparticles showed increased recruitment of anti-inflammatory macrophages to the injection site in conjunction with increased production of anti-inflammatory markers and a reduction in apoptosis. Further, the treated group showed increased axonal infiltration into the lesion, a reduction in reactive astrocyte populations and increased angiogenesis. These results translated into improved sensorimotor metrics versus the control group. CONCLUSIONS: Application of α-gal nanoparticles after spinal cord injury (SCI) induces a pro-healing inflammatory response resulting in neuroprotection, improved axonal ingrowth into the lesion and enhanced sensorimotor recovery. The data shows α-gal nanoparticles may be a promising avenue for further study in CNS trauma.