Use of Real-Time Quantitative PCR to Identify High Expressed Genes in Head and Neck Squamous Cell Carcinoma Cell Lines

Yong-Gyoo Lee,So-Young Chun,Hae-Ahm Lee,Yoon-Kyung Sohn,Ku-Seong Kang,Joung-Ok Kim,Sang-Mo Yun,Jung-Wan Kim,Hyun-Jung Jang 대한구강악안면외과학회 2006 대한구강악안면외과학회지 Vol.32 No.1

Head and neck squamous cell carcinoma(HNSCC) is the sixth most common cancer among men in the developed world affecting the tongue, pharynx, larynx and oral cavity. HNSCC is thought to represent a multistep process whereby carcinogen exposure leads to genetic instability in the tissue and accumulation of specific genetic events, which result in dysregulation of proliferation, differentiation, and cell loss and the acquisition of invasive capacity. Despite therapeutic and diagnostic progress in oncology during the past decades, the prognosis of HNSCC remains poor. Thus it seems that finding a biological tumor markers which will increase the early diagnosis and treatment monitoring rates, is of paramount importance in respect to improving prognosis. In an effort to identify gene expression signatures that may serve as biomarkers, this study several genes were selected, such as H3,3A, S100A7, UCHL1, GSTP1, PAI-2, PLK, TGFβ1 and bFGF, and used 7 HNSCC cell lines that were established various anatomical sites, and also 17 other cancer cell lines were used for control group using real-time quantitative RT-PCR and immunocytochemical analysis with a monoclonal antibody. In this study, S100A7 showed a clearly restricted occurrence in tongue originated cell line, and GSTP1 expression level in the pharynx originated cell line was very increased, relative to corresponding other cell lines. These results suggest that S100A7 and GSTP1 genes’ expression can occur during tongue and pharynx originated head and neck tumorigenesis and that genetic change is an important driving force in the carcinogenesis process. This data indicate that S100A7 and GSTP1 expression pattern in HNSCC reflect both diagnostic clue and biological marker. And this is provides a foundation for the development of site-specific diagnostic strategies and treatments for HNSCC.

Factors Influencing the Acrylamide Content of Fried Potato Products

( Yong-ik Jin ),( Kyeong-hun Park ),( Dong-chil Chang ),( Ji-hong Cho ),( Kwang-su Cho ),( Ju-sung Im ),( Su-young Hong ),( Su-jeong Kim ),( Jung-hwan Nam ),( Hwang-bae Sohn ),( Hong-seob Yu ),( Ill-m 한국환경농학회 2016 한국환경농학회지 Vol.35 No.4

BACKGROUND: Acrylamide (CAS No. 79-06-1) is known to be a carcinogenic compound, and is classified as a Group 2A compound by the International Agency for Research on Cancer (IARC, 1994). Acrylamide can be generated during the browning process via the non-enzymatic Maillard reaction of carbohydrates such as reducing sugars and of amino acids such as asparagine, both of which occur at a temperature above 120℃. Potato tubers contain reducing sugars, and thus, this will affect the safety of processed potato products such as potato chips and French fries. In order to reduce the level of acrylamide in potato processed products, it is therefore necessary to understand factors that affect the reducing sugar content of potatoes, such as environmental factors and potato storage conditions, as well as understanding factors affecting acrylamide formation during potato processing itself. METHODS AND RESULTS: Potatoes were cultivated in eight regions of Korea; For each of these different environments, soil physico-chemical characteristics such as pH, electrical conductivity, total nitrogen, available phosphate, and exchangeable cation content were measured and correlations with potato reducing sugar content and potato chip acrylamide levels were examined. The reducing sugar content in potato during storage for three months was determined and acrylamide level in potato chip was analyzed after processing. The storage temperature levels were 4℃, 8℃, or 10℃, respectively. The acrylamide content of chips prepared from potatoes stored at 10℃ or 2 0℃ for one month was analyzed and the different frying times were 2, 3, 5, and 7 min. CONCLUSION: This study showed that monitoring and controlling the phosphate content within a potato field should be sufficient to avoid producing brown or black potato chips. For potatoes stored at low temperatures, a reconditioning period (20℃ for 20 days) is required in order to reduce the levels of reducing sugars in the potato and subsequently reduce the acrylamide and improve chip coloration and appearance.

Selective addition of CXCR3+CCR4-CD4+ Th1 cells enhances generation of cytotoxic T cells by dendritic cells in vitro

Sung Hee Yoon,Sun Ok Yun,Jung Yong Park,Hee Yeun Won,Eun-Kyung Kim,Hyun-Jung Sohn,Hyun-Il Cho,김태규 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.3

Increasing importance is being given to the stimulation of Th1 response in cancer immunotherapy because its presence can shift the direction of adaptive immune responses toward protective immunity. Based on chemokine receptor expression, CXCR3+CCR4-CD4+ T cells as Th1-type cells were investigated its capacity in monocyte-derived dendritic cell (DC) maturation and polarization, and induction of antigen specific cytotoxic T lymphocytes (CTL) in vitro. The levels of IL-4, IL-5 and IL-10 were decreased to the basal level compared with high production of IFN-γ, TNF-α, and IL-2 in CXCR3+CCR4-CD4+ T cells stimulated with anti-CD3 and anti-CD28 antibodies. Co-incubation of activated CD4+ or CXCR3+CCR4-CD4+ T cells with DC (CD4+/DC or CXCR3+CD4+/DC, respectively) particularly up-regulated IL-12 and CD80 expression compared with DC matured with TNF-α and LPS (mDC). Although there was no significant difference between the effects of the CXCR3+CCR4-CD4+ and CD4+ T cells on DC phenotype expression, CXCR3+CD4+/DC in CTL culture were able to expand number of CD8+ T cells and increased frequencies of IFN-γ secreting cells and overall cytolytic activity against tumor antigen WT-1. These results demonstrated that the selective addition of CXCR3+ CCR4- CD4+ T cells to CTL cultures could enhance the induction of CTLs by DC in vitro, and implicated on a novel strategy for adoptive T cell therapy. Increasing importance is being given to the stimulation of Th1 response in cancer immunotherapy because its presence can shift the direction of adaptive immune responses toward protective immunity. Based on chemokine receptor expression, CXCR3+CCR4-CD4+ T cells as Th1-type cells were investigated its capacity in monocyte-derived dendritic cell (DC) maturation and polarization, and induction of antigen specific cytotoxic T lymphocytes (CTL) in vitro. The levels of IL-4, IL-5 and IL-10 were decreased to the basal level compared with high production of IFN-γ, TNF-α, and IL-2 in CXCR3+CCR4-CD4+ T cells stimulated with anti-CD3 and anti-CD28 antibodies. Co-incubation of activated CD4+ or CXCR3+CCR4-CD4+ T cells with DC (CD4+/DC or CXCR3+CD4+/DC, respectively) particularly up-regulated IL-12 and CD80 expression compared with DC matured with TNF-α and LPS (mDC). Although there was no significant difference between the effects of the CXCR3+CCR4-CD4+ and CD4+ T cells on DC phenotype expression, CXCR3+CD4+/DC in CTL culture were able to expand number of CD8+ T cells and increased frequencies of IFN-γ secreting cells and overall cytolytic activity against tumor antigen WT-1. These results demonstrated that the selective addition of CXCR3+ CCR4- CD4+ T cells to CTL cultures could enhance the induction of CTLs by DC in vitro, and implicated on a novel strategy for adoptive T cell therapy.

Radioimmunoscintigraphy Using IMACIS-1 in Gastrointestinal Cancer

Sohn, Hyung Sun,Kim, Sung Hoon,Lee, Jae Mun,Kim, Choon-Yul,Bahk, Yong Whee CATHOLIC MEDICAL CENTER 1990 Bulletin of the Clinical Research Institute Vol.18 No.1

Most of the diagnostic methods currently used for the detection of neoplastic masses provide indirect evidence. To obtain greater specificity in the interpretation of neoplasias by in vivo methods, the immunological approach appears to be most promising. Two problems that interfered with progress in this field were the lack of turner specific antigen and the lack of well-defined and reproducible antibodies. To improve the sensitivity and specificity of radioimmunoscintigraphy as a technique for tumor localization, the use of monoclonal antibodies, fragments of antibodies and single photon emission computerized tomography (SPECT) are reasonable. The obvious advantages of monoclonal antibodies are their homogeneity, their specificity for the immunizing antigen and the reaction with a single determinant-thus no large immunecomplexes with antigen are formed. Monoclonal antibody technique has recently provided an opportunity to reevaluate the role of nuclear medicine for the diagnosis of malignant diseases by using the immunological approach. Our first results by means of radioimmunoscintigraphy of CEA and CA 19-9 producing tumors using a cocktail of F (ab’)2 fragments of monoclonal antibodies to CA 19-9 and CEA labeled with I-131 (IMACIS-1) are reported. The aims of this investigation was to evaluate the role of immunoscintigraphy in patients with colorectal and other cancers for diagnosis of local recurrences and metastasis. This report contains results of the first 8 colorectal and pancreas cancer patients with the elevation of the level of serum CEA and/or CA 19-9. IMACIS-1 was injected intravenously during 30 minutes in 100 ml saline solution after skin test. Planar scintigrams were recorded 3, 5 and 7 days after the injection of the IMACIS-1. Anterior, lateral and posterior views of the liver as well as anterior and posterior view of the pelvis were obtained at each patients as an I-131 antibody image. We were able to localize exactly the malignant process with the double-nuclide double-compound Tc-99m I-131 (Tc-I) scintigrams. In Tc & I double-nuclide scintigraphy, computer subtraction display provided more clear localization of the tumor. We compared the results of radioimmunoscintigraphy with CT, ultrasonograms, conventional scintigrams. The results were as follows: 1. The sensitivity and specificity of radioimmunoscintigraphy using the fragments F (ab’) 2 of the cocktails of CEA and CA 19-9 monoclonal antibodies were 82% and 100% respectively. 2. Tumor detection rate was not proportionated to the level of serum tumor markers. 3. Double nuclide scintigraphy technique was essential for tumor localization as an anatomic landmark using double-nuclide scintigraphy. 4. A slow in fusion of the antibodies was necessary to prevent the formation of large immune complexes. 5. Tumor/non-tumor radioactivity was most elevated at 7 days delayed imaging. 6. Using planar scintigraphic technique of I-131 labeled monoclonal antibodies are possible for imaging most of the tumors.

The etiology and natural course of Dermatomyositis/polymyositis (DM/PM) patients with acute respiratory failure

( Sung Jun Chung ),( Yoomi Yeo ),( Hyun Lee ),( Tai Sun Park ),( Dong Won Park ),( Ji-yong Moon ),( Sang-heon Kim ),( Tae-hyung Kim ),( Jang Won Sohn ),( Ho Joo Yoon ) 대한결핵 및 호흡기학회 2018 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.126 No.-

Purpose: The etiology and natural course of Dermatomyositis/polymyositis (DM/PM) patients with acute respiratory failure have not been well studied. Methods: A total of 36 patients who were admitted to medical intensive care unit between January, 2002 to August, 2018 in Hanyang Medical center due to acute respiratory failure were retrospectively evaluated. Results: Of the 36 patients, 19 had acute exacerbation (AE) of DM/PM-associated interstitial lung disease and 17 had pneumonia. The overall in-hospital mortality was 44.4% (16/36) including 9 (52.6%) in patients with AE of DM/PM-associated ILD and 7 (41.2%) in patients with pneumonia. There was no significant difference in-hospital mortality between the two groups (P=0.492). Conclusion: The treatment outcomes in DM/PM patients who developed respiratory failure was relatively poor. There was no significant difference in-hospital mortality according to etiologies.

The Effects of Monetary Policy on Individual Welfares

Sung Jin Kang,Yong Woon Chung,Sang Hak Sohn 한국경제연구학회 2013 Korea and the World Economy Vol.14 No.1

Monetary policy affects heterogeneously the individual welfare even though its main goals such as income growth and economic stability are well accomplished. This is because macroeconomic policy has different impact on individuals according to their income and other characteristics. Accordingly, monetary policy changes the status of income distribution and poverty. By using Panel System GMM estimation for household and province-level data of Korea from 1997 to 2007, this study investigates short and long-term effects of monetary policy on income distribution as well as poverty. The estimation results are as follows. First, real interest rate and poverty are positively correlated while real interest rate does not have significant effects on income distribution. Second, income growth reduces poverty and improves income distribution. Third, inflation reduces poverty while inflation improves income distribution in the short-term but has no significant effects on income distribution in the long-term. Fourth, long-term effects of monetary policy on poverty gap are greater than short-term effects by 60%. And long-term effects of monetary policy on poverty severity are larger than short-term effects by 53%. But for the Gini coefficient, effects of monetary policy do not last long because the coefficient’s auto-correlation term is not significant.

Vasoconstriction Potency Induced by Aminoamide Local Anesthetics Correlates with Lipid Solubility

Sung, Hui-Jin,Ok, Seong-Ho,Sohn, Jin-Young,Son, Yong Hyeok,Kim, Jun Kyu,Lee, Soo Hee,Han, Jeong Yeol,Lim, Dong Hoon,Shin, Il-Woo,Lee, Heon-Keun,Chung, Young-Kyun,Choi, Mun-Jeoung,Sohn, Ju-Tae Hindawi Publishing Corporation 2012 Journal of biomedicine & biotechnology Vol.2012 No.-

<P>Aminoamide local anesthetics induce vasoconstriction <I>in vivo</I> and <I>in vitro</I>. The goals of this <I>in vitro</I> study were to investigate the potency of local anesthetic-induced vasoconstriction and to identify the physicochemical property (octanol/buffer partition coefficient, pKa, molecular weight, or potency) of local anesthetics that determines their potency in inducing isolated rat aortic ring contraction. Cumulative concentration-response curves to local anesthetics (levobupivacaine, ropivacaine, lidocaine, and mepivacaine) were obtained from isolated rat aorta. Regression analyses were performed to determine the relationship between the reported physicochemical properties of local anesthetics and the local anesthetic concentration that produced 50% (ED<SUB>50</SUB>) of the local anesthetic-induced maximum vasoconstriction. We determined the order of potency (ED<SUB>50</SUB>) of vasoconstriction among local anesthetics to be levobupivacaine > ropivacaine > lidocaine > mepivacaine. The relative importance of the independent variables that affect the vasoconstriction potency is octanol/buffer partition coefficient > potency > pKa > molecular weight. The ED<SUB>50</SUB> in endothelium-denuded aorta negatively correlated with the octanol/buffer partition coefficient of local anesthetics (<I>r</I><SUP>2</SUP> = 0.9563; <I>P</I> < 0.001). The potency of the vasoconstriction in the endothelium-denuded aorta induced by local anesthetics is determined primarily by lipid solubility and, in part, by other physicochemical properties including potency and pKa.</P>

Battery performance of PMMA-grafted PE separators prepared by pre-irradiation grafting technique

Sung-Jin Gwon,최재학,Joon-Yong Sohn,Youn-Mook Lim,Young-Eon Ihm,Young-Chang Nho 한국공업화학회 2009 Journal of Industrial and Engineering Chemistry Vol.15 No.5

Poly(methyl methacrylate)-grafted polyethylene (PE-g-PMMA) separators were prepared by preirradiation grafting technique of methyl methacrylate onto a commercial polyethylene separator. The prepared separatorswere characterized by using charge/discharge (C/D) cycling test, AC impedance, and thermal stability analyses. Thermal shrinkage (TS) of the PE-g-PMMA separators decreased with an increasing degree of grafting up to 70% above which it was saturated. The PE-g-PMMA separators showed a better oxidation stability on the anode up to 5 V and a better cycle life performance than the original PE separator. These characteristics make the prepared separators suitable for applications in high voltage secondary lithium batteries.

The Prevalence of and Factors Associated with Influenza Vaccination in Patients with Asthma in Korea

( Sang Hyuk Kim ),( Sungmin Zo ),( Hwasik Jung ),( Saerom Kim ),( Ho Joo Yoon ),( Jang Won Sohn ),( Tae-hyung Kim ),( Sang-heon Kim ),( Ji-yong Moon ),( Dong Won Park ),( Tai Sun Park ),( Hye Yun Park 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

Background Patients with asthma are at high risk of hospitalization and death from influenza infection, which Results in an additional burden on the medical system. However, despite the crucial role of influenza vaccination in preventing influenza infection and its complication, detailed epidemiologic data regarding influenza vaccination among patients with asthma is lacking. This study aimed to investigate the rate of influenza vaccination and factors associated with the vaccination in patients with asthma using nationally representative survey data. Methods Among 72,843 participants of the Korea National Health and Nutrition Examination Survey between 2010 and 2019, 760 patients with self-reported physician-diagnosed asthma were included in this study. The outcome was the influenza vaccination rate assessed by questionnaire. Logistic regression analysis was performed to evaluate factors associated with not being vaccinated in patients with asthma. Results The overall influenza vaccination rate was 63.7% in patients with asthma and there was no significant change in the vaccination rate (range 58.7%-70.0%) during the study period. In multivariable logistic regression analysis, younger age (adjusted odds ratio [aOR] 1.11, 95% confidence interval [CI] 1.07-1.16, p < 0.001), lower income (aOR 2.76, 95% CI 0.77-3.80, p = 0.040), and the presence of respiratory symptoms (aOR 2.56, 95% CI 1.22-5.36, p = 0.012) were associated with the increased odds of being unvaccinated for influenza. Conclusion The vaccination rate in patients with asthma was stable, ranging from approximately 60% to 70% between 2010 and 2019 in Korea. As patients with younger age, lower income, or respiratory symptoms might be non-adherent to influenza vaccination, more attention is needed in these patients to increase the influenza vaccination rate. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, Information and Communications Technologies (MSIT) (NRF-2020R1F1A1070468 and NRF-2021M3E5D1A01015176).

Biosafety assessment of Golden rice (PAC): 1.Progeny stability study through multiple generations

Sung Dug Oh,Ki Jong Lee,Soo In Sohn,Jang Yong Lee,Tae Hoon Ryu,Jae Kwang Kim,Sun Hwa Ha,Seok Cheol Suh,Jong Sug Park 한국육종학회 2011 한국육종학회 학술발표회 발표요지 Vol.43 No.-