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Kwak So-Hyung,Kim Hayeong,Yun Hyeli,Lim Juho,Kang Dong-Hyun,Kim Doman 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.6
Canine parvovirus type 2 (CPV-2) has high morbidity and mortality rates in canines. Nonstructural protein 1 (NS1) of CPV-2 has endonuclease activity, initiates viral DNA replication, and is highly conserved. Thus, it is a promising target for antiviral inhibitor development. We overexpressed a 41.9 kDa active recombinant endonuclease in Escherichia coli and designed a nicking assay using carboxyfluorescein and quencher-linked ssDNA as substrates. The optimal temperature and pH of the endonuclease were 37°C and pH 7, respectively. Curcumin, bisdemethoxycurcumin, demethoxycurcumin, linoleic acid, tannic acid, and α-tocopherol inhibited CPV-2 NS1 endonuclease with IC50 values of 0.29 to 8.03 μM. The extracted turmeric, yerba mate, and sesame cake suppressed CPV-2 NS1 endonuclease with IC50 values of 1.48, 7.09, and 52.67 μg/ml, respectively. The binding affinity between curcumin, the strongest inhibitor, and CPV-2 NS1 endonuclease by molecular docking was −6.4 kcal/mol. Curcumin inhibited CPV-2 NS1 endonuclease via numerous hydrophobic interactions and two hydrogen bonds with Lys97 and Pro111 in the allosteric site. These results suggest that adding curcuminoids, linoleic acid, tannic acid, α-tocopherol, extracted turmeric, sesame cake, and yerba to the diet could prevent CPV-2 infection.
So-Hyun Lee,Dong-Hoon Kwak,Hyung-Keun You,Beom-Su Kim,Kyung-A Hwang,Byoung-Boo Seo,Hwi-cheul Lee,Jae-Sung Ryu,So-Dam Lee,Ju-Taek Lee,Yoon-Ju Na,Su-Bin Lee,Kyu-Tae Chang,Young-Kug Choo 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Gangliosides, sialic acid-conjugated glycosphingolipids, are involved in cell proliferation and differentiation. This study investigated the possible role of gangliosides in the dopaminergic neuron-like cells of human mesenchymal stem cells (hMSCs). We have reported the in vitro conditions for inducing hMSCs into dopaminergic neuron-like cells. hMSCs were induced in vitro to become dopaminergic neuron-like cells in 12 days by using a cocktail that includes sonic hedgehog and fibroblast growth factors. First, we investigated the characterization of hMSCs through FACS analysis. The mesenchymal stem cell specific markers, CD44 and CD105, were expressed in hMSCs while the hematopoetic markers, CD45 and CD117 were not. The cells developed a neuronal morphology expressing the neuronal markers Map2 and βⅢtubulin. RT-PCR analysis revealed the expression of dopamine specific genes such as TH, Pitx3, Nurr1 and DA transporter. Immunocytochemistry showed that hMSCs expressed GM3, GM2, GD1a, GD3 and GT1b based on tyrosine hydroxylase (TH) expression. Specifically, a significant decrease in GM3 and GT1b expression was observed during dopaminergic neuron differentiation. These results suggest that ganglioside GT1b may play a role in the dopaminergic neuron differentiation and development process of hMSCs.
Sohn, So-Dam,Kim, Su Hwan,Kwak, Sang Kyu,Shin, Hyung-Joon Elsevier 2019 APPLIED SURFACE SCIENCE - Vol.467 No.-
<P><B>Abstract</B></P> <P>Controlling defects is one of the basic strategies for tailoring electronic structure of materials, which has not been explored that much yet for organic-inorganic hybrid systems. In this study, we investigated the control of work function of oxide electrode by defect-associated adsorption of molecules at the single-molecule level by means of scanning tunneling microscopy and first principle calculations. The equilibrium adsorption configuration of monoethanolamine (MEA, HO(CH<SUB>2</SUB>)<SUB>2</SUB>NH<SUB>2</SUB>), an effective coating for lowering the work function of an oxide electrode, varies as a function of surface coverage at TiO<SUB>2</SUB>(110) surfaces. Our results showed that defects at the oxide surface and intermolecular interactions dominate the stable configuration of adsorbates as well as work function of the system. The dissociative adsorption at <SUB> O v </SUB> was found to be more efficient at lowering the work function of TiO<SUB>2</SUB>(110) surface, suggesting that defect control can be used to improve the performance of organic-inorganic hybrid systems.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Both defect and adsorbates at surface modify the work function of the oxide substrate. </LI> <LI> MEA molecules adsorb at oxygen vacancy on TiO<SUB>2</SUB>(110) surface in a dissociated form. </LI> <LI> On perfect TiO<SUB>2</SUB>(110) surfaces, MEA molecules adsorb at Ti rows. </LI> <LI> The largest reduction in work function can be achieved by dissociative adsorption of MEA at oxygen vacancy. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>The use of well-controlled defects provides an alternative approach to better performance of hybrid organic-inorganic devices.</P> <P>[DISPLAY OMISSION]</P>