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Lung Cancer P-002 : MicroRNA-7-5p’s Role in Growth and Metabolism of Lung Cancer
( Sin Yung Woo ),( Daeun Kang ),( Su Yel Lee ),( Seong-lan Yu ),( Se Jin Park ),( Jin Suk Kim ),( Shin Yeop Lee ),( In Beom Jeong ),( Sun Jung Kwon ),( Wan Jin Hwang ),( Chang Ryul Park ),( Ji Woong S 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-
OGlcNAc Transferase (OGT) is a complementary enzyme that regulates O-linked N-acetylglucosamine (O-GlcNAc) and plays a critical role in various cancer phenotypes including invasion, migration, and metabolic reprogramming. In this study, OGT is the predicted target of miR-7-5p. To identify miR-7-5p’s role in cell growth and metabolism, we treated various lung cancer cell lines with miR-7-5ps and performed qRT- PCR and western blot to demonstrate that OGT is the target of miR-7-5p. Furthermore, relative luciferase assay was done to show that miR-7-5p directly regulates OGT. To define the role of mir-7-5p in cancer cell metabolism, we performed LDH assay, glucose uptake, and mitochondrial ATP synthase inhibitor assay. Induced overexpression of miR-7-5p in lung cancer cells decreased aerobic glycolysis as is demonstrated by reduced LDH production, glucose uptake, and glycolysis. Increased O-GlcNAcylation contributed to the augmentation of lung cancer cell metabolism. In the present study, inhibition of OGT by miR7-5p decreased the growth and metabolism of lung cancer. These findings suggest that modulating OGT activity may present a potential therapeutic strategy for cancer.