강둑여과수를 이용한 정수처리 공정별 수질의 변이원성 평가

이순화,박영규,이철희 嶺南大學校 環境問題硏究所 1999 環境硏究 Vol.18 No.2

ABSTRACT Much of the public health concern about this topic originated after the detection of carcinogenic and/or mutagenic compounds in surface water including drinking waters. Such presence of mutagenic substances in river water brings about the possibility of causing hazardous effects on not only aquatic organisms but also human health itself. To secure the safety of drinking water, it should be necessary to monitor these micropollutants. Mutagenicity Test, in this bioassay, should produce at least twice the number of revertants as concurrent negative controls and provide a clear dose-response relationship. In this result, Nakdong River was polluted by the frame-shift mutagenic and hydrophilc compounds. To improve its quality, surface water may be treated in different ways. Several processes, such as bank filtration, coagulation, ozonation and activated carbon adsorption can be used to remove natural and sythetic organic compounds.

强制循環에 의한 湖水의 溶存酸素 變化에 關한 硏究

李淳和,金庸桓 嶺南大學校 環境問題硏究所 1994 環境硏究 Vol.13 No.2

호수의 수질을 개선하기 위한 방법으로 강제순환장치를 도입한 인공호 수질의 변화를 검토하였다. 강제순환장치의 도입 전·후 수온의 변화로 비교하여 강제순환에 의한 수질 변화를 예측한 후, 이화학적인 수질의 변화를 검토하였으며, 호수의 수질 변화에 가장 중요인자인 용존산소의 변화를 표면층에서 저니층까지 검토하여 재폭기에 영향을 주는 강제순환의 효과를 제시하였다. 이때 增間댐과 作名댐의 재폭기 계수는 각각 2.612와 2.984g/㎥/day로 나타났다. Limnological survey and several field experiment were done for estimating the effect of artificial circulation. The changes of vertical distribution of dissolved oxygen concentration resulting from artificial circulation were different from the changes of water temperature. The major factors to control the dissolved oxygen concentration under the artificial circulation were suggested to role of reaeration. The reaeration coefficient, R with the artificial circulation for Masuma and Sakuna Dams were estimated about 2.612 and 2.984g/㎥/day, respectively.

인공호수 저니로부터 영양염 용출변화에 관한 연구

이순화,김용환 嶺南大學校 環境問題硏究所 1996 環境硏究 Vol.15 No.2

호수내에 있어서 영양염의 변화에 미치는 영향을 고찰하기 위하여 저니에서의 영양염의 용출영향을 보면, 수온에 따른 저니에서의 영양염 용출실험의 결과, 혐기성 상태에서 영양염 용출은 10℃, 20℃, 30℃로 변화 시켰을때, TIN은 2.70, 3.31, 3.78 g/㎡/d로 각각 용출되었고, T-P의 용출량은 0.61, 0.74, 0.76 g/㎡/d였으며, 수온이 높을수록 용출량이 높게 나타나 온도의 의존성이 있다고 판단된다. pH에 따른 저니에서의 영양염 용출실험을 한 결과, 혐기성 상태조건에서 PO_4-P의 용출은 pH가 높을수록 비례적으로 용출량이 많았으나 TIN의 경우는 pH의 의존성이 인의 경우보다 낮게 나타났다. DO에 따른 용출량 변화는, 호기계에서 보다 혐기계인 상태에서 용출이 많이 일어나고 있으며, 호기계에서 용출이 적은 이유는 DO가 저층에 있는 저니에서의 영양염 용출이 일어나는 것을 억제했기 때문이라고 판단된다. This Study focuses on the nutrient release in the artificial lake sediment. Results show that TIN release rates were 2.70, 3.31, 3.78 g /㎡/d, and those of T-P were 0.61, 0.71, 0.76 g/㎡/d at the 10℃, 20℃, 30℃, respectively, indicating the effects of temperature on nutrient release in the sediments. It can also be shown from the experimental result that the release rate PO_4-P increases with increasing pH while that of TIN is not so much affected by the pH, and the nutrient release is more active in anaerobic condition than in aerobic condition Indicating that DO suppresses the nutrient release in the lake sediments.

변이원성을 이용한 낙동강 중류수계의 수질평가

이순화,박영규,이철희 嶺南大學校 環境問題硏究所 1998 環境硏究 Vol.17 No.2

강제순환에 의한 호수 중의 무기질소변화

이순화 國立 昌原大學校 産業技術硏究所 1992 産技硏論文集 Vol.6 No.-

In generally, water quality of eutrophic zone in lake must decrease nutrients for example phosphorous and nitrogen. This paper is concerned with limnological survey and several field experiment were done for estimating the effect of artificial circulation. Internal nitrogen loading was estimated by the loaboratiory corer method. Sedimentation of nitrogen and suspended solid were determined using sediment traps at bottom. NH₄-N release concentrations of surface on bottom were large more than removed sediment from 5mm to 20mm, However, after cover oxidized sedimentation by artificial circulation decreased NH₄-N release concentration, and nutrients concentrations of interstitical water in sediment were the reverse of the change NH₄-N release concentration by nitrification. TIN release fluxes from sediment lowered from 15.3㎎/㎡/day by 10.7㎎/㎡/day by artificial circulation in Masuma lake.

고체상 추출법을 이용한 Phthalate Esters의 분석방법 연구

홍성희,한개희,이찬형,이순화 한국환경과학회 2003 한국환경과학회지 Vol.12 No.1

The study was carried out to evaluate the new analytical method of phthalate esters(diethyphthalate, di-n-butylphthalate, butylbenzylphthalate, bis(2-ethylhexyl)phthalate), one of the endocrine disruptors, which were performed by GC/MS-SIM(seleted ion monitoring). The phthalate esters were extracted from water samples using solid-phase extraction on C_18 columns. It investigated that the extraction recovert rate of phthalate esters with different solvents and solvent volume. The optimal solvent was dichloromethane and proper volume of dichloromethane for recovert of phthalate esters was 4 mL. There were good linearities(above R^2=0.9975) in the range 0.01∼0.50㎎/L,and the detection limits were below 0.01∼0.03㎍/L.The recovert rates,RSD and MDLs for phthalate esters were 80∼114%,5.0∼8.1% and 0.03∼0.11㎍/L,respectively. This method shows a good precision of phthalate esters.

Dependency of Mutagenicity on Micropollutants - A Case Study on Nakdong River Water -

( Shun Hwa Lee,Yung Kyu Park,Chul Hee Lee,Ji Hoon Kim,Su Hyon Kim ) 한국물환경학회 1997 제6차 국제수질보전학회 아시아 태평양지역 학술회의 Vol.2 No.-

A study on the Fractionation of Dissolved Organic Carbon in Lake and River Waters

Lee, Shun-Hwa,Kim, Yong-Hwan,Park, Jong-Woong 경산대학교(대구한의과대학) 1999 環境硏究 Vol.4 No.1

Dissolved organic carbon(DOC) in Lake Kasumigaura, its inflowing rivers and several DOC sources in its catchment area was fractionated using three resin adsorbents into classes : aquatic humic substances (AHS=humic acid+fulvic acid), hydrophobic neutrals (HoN), hydrophilic acids(HiA), bases(BaS) and hydrophilic neutrals(HiN). The DOC fractionation produced interesting fraction distributions that were significantly different depending on the origin of sample. AHS and HiA prevailed over AHS in the lake while AHS and HiA existed at almost the same level in the rivers. AHS seems to be a more important component in river water than lake water. The dominance of organic acids was also observed for the waters of the DOC sources : forest stream(FS), Plowed field percolate(PFP), paddy field outflow(PFO), domestic sewage(DS) and sewage treatment plant effluent(STPE). However, the DOC fraction distributions reulted in more different distribution patterns. AHS dominated FS and PFP. HiA dominated PFO, DS and STPE. Only DS contained a significant amount of HoN, which may be due to the presence of LAS-like DOC. The ultraviolet absorbance over D0C(UV:D0C) ratio was found to a useful indicator for reflecting the characteristics of DOC, AHS and HiA and the origin of sample. The UV:D0C ratio in both lake and river waters was in the order of AHS, DOC, HiA. The lake water exhibited the lower ratios in DOC, AHS and HiA than the river waters did. Autochthonous DOC and AHS, which have been reported to have a lower UV:D0C ratio, may contribute significantly those in the lake. For AHS dominated FS and PFP, the ratio was in the order of DOC, AHS, HiA and DOC, HiA, AHS, respectively. PFO had the greatest UV:D0C ratio of AHS among the samples studied, indicating that the paddy field added up more pedogenic AHS to its overlaying water. For DS, the UV:D0C ratios of DOC, AHS and HiA were the lowest among fhe sanmles. Its AHS showed an UV:D0C ratio even less than those of HiA in all the other sarmp1es.

Annealing control primer system identifies differentially expressed genes in blastocyst-stage porcine parthenotes

Lee, Hwa Young,Cui, Xiang-Shun,Lee, Kyung-Ah,Kim, Nam-Hyung Cambridge University Press 2006 Zygote Vol.14 No.1

<P>There is very little information available on stage-specific gene expression during early embryo development, particularly in the pig. Here, we accurately identified the genes that are specifically or prominently expressed in parthenogenetic porcine blastocysts as compared with 2-cell stage embryos. We accomplished this by using a PCR technology regulated by annealing control primers (ACPs). By utilizing 120 ACPs, a total of 46 expressed sequence tags (ESTs) of genes that are differentially expressed in blastocysts as compared with 2-cell stage embryos were cloned and sequenced. The cloned genes or ESTs all exhibited significant sequence similarity with known genes or ESTs of other species. Of the known genes, six genes [renin-binding protein (RNBP), BMDP, solute carrier family 25 (SLC25A6), MTHFD1, TRK-fused gene (TFG), spermidine synthase (SRM)] were selected and their stage-specific expression levels in porcine parthenotes were determined by real-time quantitative polymerase chain reaction at the 1-, 2-, 4-cell, morula and blastocyst stages. While RNBP, BMDP, SLC25A6, MTGFD1 and SRM were highly expressed only at the blastocyst stage, TFG was highly expressed at the 1-cell stage, then declined after genomic activation, high levels of expression being again detected at the morula and blastocyst stages. This analysis suggests that the ACP system is an effective tool for use in the identification of stage-specific genes in small numbers of porcine parthenotes. Examination of the genes differentially expressed in the blastocyst, which we have identified here, will provide insight into the molecular basis of preimplantation development.</P>

WATER QUALITY EVALUATION OF NAKDONG RIVER BY MUTAGENICITY

Shun Hwa Lee,Yung Kyu Park,Chul Hee Lee,Ji Hoon Kim 대한환경공학회 1999 Environmental Engineering Research Vol.4 No.3