Effect of Daylength on the Panicle Exsertion of Panicle Enclosing Gamadi Rice Cultivar

Shrestha,G,L,M,H,Heu,S,Z,Park 한국작물학회 1981 한국작물학회지 Vol.26 No.2

Metabolic engineering in E. coli for production of C-glycosylated flavones and their physio-chemical studies.

Shrestha,DipeshDhakal,RameshPrasadPandey,Jae Kyung Sohng 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.07

수도의 묘대일수가 절간신장에 미치는 영향

Gyan L. Shrestha갼 랄 슈레스터 韓國作物學會 1985 Korean journal of crop science Vol.30 No.2

네팔에서 보급단계로 진전된 수도 3계통 IET 7251, BG 400-1 및 B 44 b-50-2-2-5-1 을 6월 5일 파종하여 10일, 20일, 30일, 40일, 50일 및 60일 묘를 이앙하고 그들의 출수일수와 절간신장을 조사하여 다음과 같은 결과를 얻었다. 1. 간장은 30일묘 이앙구에서 가장 길었고 그보다 묘대일수가 길어짐에 따라 현저하게 간장이 단축되었다. 2. 육면으로 감별이 가능한 절수도 30일묘 이앙구에서 가장 많았는데 40일묘까지는 큰 차가 없었으나 그보다 묘대기간이 길어지면 유의하게 절수가 감소 되었다. 3. 간장은 40일묘 이앙구에서 가장 길었다. 4. 출수 및 성숙까지의 일수는 이앙이 늦어질수록 증가되었다. Three different improved pre-release rice varieties, when transplanted at different ages of seedlings, showed that 40 day old seedlings produced the longest panicle, whereas 30 day old seedlings produced the highest culm length among treatments in all treated varieties. 40 day or older seedlings reduced the culm length remarkably mainly due to decrease in lower internodes in IET7251 and BG400-1, and due to upper as well as lower internodes in B44b-50-2-2-5-1. 30 day old seedlings produced maximum number of visible internodes. Heading as weil as maturity was delayed with increasing age of seedling.

In vitro에서 큰번데기 동충하초 자실체형성의 불안정성

부샨 쓰레스타 외 한국버섯학회 2004 한국버섯학회지 Vol.2 No.3

Traditionally, Cordyceps species have been used as a part of herbal medicine in Oriental countries, including Korea for internal health, vigor and to cure different diseases related to heart, lung etc. In recent years, research on artificial fruiting of some species of the genus Cordyceps including C. militaris has been carried out extensively because of their medicinal value. Instability observed in the in vitro fruiting of C. militaris is reported in the present study. 전통적으로 Cordyceps종은 한국을 포함한 동양에서 건강증진과 심장과 폐에 관련된 질환을 치료하는 약용식물의 하나로 사용되어왔다. 최근에 동충하초의 약리적 가치 때문에 큰번데기 동충하초를 포함한 몇가지 Cordyceps종의 인공재배에 관한 연구가 활발하게 이루어지고 있다. 본 연구에서는 큰번데기 동충하초를 in vitro에서 인공재배한 결과 자실체 형성이 불안정한 것을 관찰하였기에 보고하고자 한다.

Assessment on Scaling-Up of Mini-Grid Initiative: Case Study of Mini-Grid in Rural Nepal

Pramish Shrestha,Ashish Shrestha,Namrata Tusuju Shrestha,Antonis Papadakis,Ramesh Kumar Maskey 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.1

A majority of Nepalese population are living in rural areas, where the electricity access by grid extension is not feasible. Development of micro-hydropower in the isolated state is one of the solutions to provide the electricity to these areas. However, the micro-hydro systems are facing numerous challenges, such as high investment cost, low reliability, low load factor and protection issues. For this reason, the concept of mini-grid has been developed to address the discussed challenges. In this study, a case study of a mini-grid, which is formed by interconnecting six isolated Micro Hydropower Plants (MHPs) with an installed capacity of 107 kW at a transmission voltage of 11 kV, is considered. This study performs the technical and financial analysis, to study the possibility for scaling up the system. Based on different factors, optimum models have been selected, considering a numbers of different cases including cost–benefit and sensitivity analysis. The mini-grid connection of several isolated MHPs is technically possible, but the financial feasibility depends upon various factors, such as the distance between the MHPs, end-use promotion, total capacity of MHPs, future load growth, promotion mechanisms, power trading with utility, etc. The findings show that sustainable operation of mini-grid requires the local capacity building, coordination, and understanding among community cooperatives.

PE-163: Association Liver Enzymes with Blood Pressure in Diabetic Patients

( Shrestha Rojeet ),( Shrestha Nirajan ),( Bhatt Nirmal Prasad ),( Dahal Sudimna ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Gamma-glutamytransferase (GGT) is routinely used laboratory investigation for liver damage and alcohol consumption. Serum GGT concentration is determined by body fat content, lipid and glucose levels, alcohol consumption and various medications. Several lines of evidence show that serum GGT is associated with cardiovascular and all-case mortality. It is also a marker of oxidative stress. Although serum GGT is associated with hypertension, the correlation between GGT and blood pressure has not been well established. The aim of the present study was to investigate the association between serum GGT and blood pressure in type 2 diabetic patients (T2DM). Methods: A total number of 105 patients with T2DM, who attended Nepal Medical College Teaching Hospital, Kathmandu, Nepal, were recruited for the study. There were 39 males (mean age: 56.6±5.2 years) and 66 females (mean age: 49.6±6.3). The patients with history of liver disease and alcohol intake were excluded from the study. After basic anthropometric measurement, fasting venous blood was collected and subjected for the estimation of liver enzymes. All data were expressed as mean± SD. p-values less than 0.05 were considered as statistically significant. Results: Although serum ALT and AST levels were significantly elevated within the normal range in T2DM compared to control, they are not correlated with systolic blood pressure (SBP) (r= 0.07 and 0.128, respectively) and diastolic blood pressure (DBP) (r= 0.08 and 0.02, respectively) in both men and women. However, serum GGT levels within their normal range were positively correlated with SBP (r=0.4, p<0.01) and DBP (r=0.275, p<0.05) in women. Interestingly, neither of these liver enzymes was correlated with blood pressure among men diabetic patients. Conclusions: These findings suggest that GGT may be linked with blood pressure balance in women and evaluating the levels of GGT could help in the monitoring of hypertension in diabetic individuals.

Bio-inspired hybrid scaffold of zinc oxide-functionalized multi-wall carbon nanotubes reinforced polyurethane nanofibers for bone tissue engineering

Shrestha, Bishnu Kumar,Shrestha, Sita,Tiwari, Arjun Prasad,Kim, Jeong-In,Ko, Sung Won,Kim, Han-Joo,Park, Chan Hee,Kim, Cheol Sang Elsevier 2017 Materials & Design Vol.133 No.-

<P><B>Abstract</B></P> <P>In this study, we prepared nanotopographical polyurethane (PU)-based bioactive scaffolds that incorporated uniformly dispersed functionalized multi-wall carbon nanotubes (<I>f</I>MWCNTs) and zinc oxide (ZnO) nanoparticles (NPs) using an electrospinning technique. We found that well dispersed <I>f</I>MWCNTs along with ZnO NPs reinforced PU fibers demonstrated significant improvement in mechanical strength, hydrophilicity, thermal stability, electrical conductivity, degradability, biomineralization, and biocompatibility. Inspired by the exciting nature of biopolymeric composite (PU/ZnO-<I>f</I>MWCNTs) membranes, these hybrid scaffolds offer extensive interest to tissue engineering as a potential biomedical application. The specific bioactive properties and cell-biomaterial interaction of electrospun scaffold containing 0.2wt% ZnO with 0.4wt% <I>f</I>MWCNTs were found to demonstrate anti-bacterial activity and cytocompatibility. Furthermore, the highly charged density, large surface-to-volume ratio, and more functional groups in <I>f</I>MWCNTs integrated on the scaffolds promote osteogenic differentiation of pre-osteoblast (MC3T3-E1) cells. Therefore, the novel as-prepared multifunctional electrospun fibrous scaffold could suggest new avenues for exploration as promising osteoproductive and osteoinductive biomaterials that offer great benefit to bone tissue engineering.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Functionalized multi-wall carbon nanotubes (<I>f</I>MWCNTs) within nanofiber enhance the electrical conductivity of scaffolds. </LI> <LI> The <I>f</I>MWCNTs (0.4 wt%) in scaffolds show good antibacterial activity. </LI> <LI> Interaction of zinc oxide and <I>f</I>MWCNTs with simulated body fluid resulting nucleation of calcium phosphate. </LI> <LI> The bioscaffolds exhibit excellent platform for osteogenic differentiation of pre-osteoblastic cells. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Investigation of the molecular mechanism of δ-catenin ubiquitination: Implication of β-TrCP-1 as a potential E3 ligase

Shrestha, H.,Yuan, T.,He, Y.,Moon, P.G.,Shrestha, N.,Ryu, T.,Park, S.Y.,Cho, Y.C.,Lee, C.H.,Baek, M.C.,Cho, S.,Simkhada, S.,Kim, H.,Kim, K. Elsevier Biomedical Press 2016 Biochimica et biophysica acta, Molecular cell rese Vol.1863 No.9

Ubiquitination, a post-translational modification, involves the covalent attachment of ubiquitin to the target protein. The ubiquitin-proteasome pathway and the endosome-lysosome pathway control the degradation of the majority of eukaryotic proteins. Our previous study illustrated that δ-catenin ubiquitination occurs in a glycogen synthase kinase-3 (GSK-3) phosphorylation-dependent manner. However, the molecular mechanism of δ-catenin ubiquitination is still unknown. Here, we show that the lysine residues required for ubiquitination are located mainly in the C-terminal portion of δ-catenin. In addition, we provide evidence that β-TrCP-1 interacts with δ-catenin and functions as an E3 ligase, mediating δ-catenin ubiquitin-proteasome degradation. Furthermore, we prove that both the ubiquitin-proteasome pathway and the lysosome degradation pathway are involved in δ-catenin degradation. Our novel findings on the mechanism of δ-catenin ubiquitination will add a new perspective to δ-catenin degradation and the effects of δ-catenin on E-cadherin involved in epithelial cell-cell adhesion, which is implicated in prostate cancer progression.

π-Conjugated polyaniline-assisted flexible titania nanotubes with controlled surface morphology as regenerative medicine in nerve cell growth

Shrestha, Bishnu Kumar,Shrestha, Sita,Baral, Ek Raj,Lee, Ji Yeon,Kim, Beom-Su,Park, Chan Hee,Kim, Cheol Sang Elsevier 2019 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.360 No.-

<P><B>Abstract</B></P> <P>Biologically active conjugated polymers, for example polyaniline (PANI), have drawn attention as emerging materials for applications in bio-medical implant devices, due to their inherent abilities with regard to charge-carrier properties, and their ability to immobilize biomolecules or proteins. Herein, we report an electrocoating of PANI on titania nanotubes (TNTs) via electrochemical oxidation of aniline with PANI layers of appropriate thickness (∼274 nm). Uniform titanium oxide nanotubes were first developed from titanium (Ti) foil through an anodization process, followed by calcination to obtain high purity TNTs vertically aligned on a Ti substrate. These had a large surface area, controllable tube height and diameter, and were highly biocompatible, and doping with PANI further improved their properties, like being antibacterial, having a lower charge transfer resistance (22.51 Ω) and strong anti-corrosion behavior (<I>E<SUB>corr</SUB> </I> ∼ − 184 mV, <I>I<SUB>corr</SUB> </I> ∼ 9.7 × 10<SUP>−7</SUP> Amp). <I>In vitro</I> experiments revealed that the cellular functions of PC12 and S42 cells on TNTs-PANI scaffolds show characteristic improvement in proliferation and differentiation owning to approach neuronal cells activation associated with axonal growth and migration in the peripheral nervous system (PNS). Thus, the flexible bioactive substrate is capable of stimulating neuronal cells, and can inspire neural transduction through direct neural interfaces.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A highly conductive electroactive polyaniline (PANI) was electrodeposited uniformly on titania nanotubes (TNTs) substrate. </LI> <LI> Titania nanotubes-polyaniline (TNTs-PANI) showed antibacterial activity and corrosion resistivity. </LI> <LI> The biointerface TNTs-PANI was used as basal substrate for PC12 cells regeneration and differentiation. </LI> <LI> The flexible TNTs-PANI substrate accelerated the neurites outgrowth of PC12 cells. </LI> </UL> </P>

Electrodeless coating polypyrrole on chitosan grafted polyurethane with functionalized multiwall carbon nanotubes electrospun scaffold for nerve tissue engineering

Shrestha, Sita,Shrestha, Bishnu Kumar,Kim, Jeong In,Won Ko, Sung,Park, Chan Hee,Kim, Cheol Sang Elsevier 2018 Carbon Vol.136 No.-

<P><B>Abstract</B></P> <P>Herein, we engineered a self-electrical stimulated double-layered nerve guidance conduit (NGC) assembled from electrospun mats with an aligned oriented inward layer covered with a random oriented outer layer. The biomimetic NGC can be achieved from chitosan grafted polyurethane with well-dispersed functionalized multiwall carbon nanotubes (<I>f</I>MWCNTs) nanofibrous mats after a uniform coating of polypyrrole (PPy). The structural framework of interconnected NGC exhibited cellular biomaterial interface and improved the physicochemical properties, including electrical conductivity, mechanical strength, and cytocompatibility, serving as natural hosting substrate to natural extracellular matrices (ECM) for vital roles in nerve tissue engineering. The regrowth, proliferation, and migration, of Schwann cells (S42) and the differentiation of rat pheochromocytoma cells (PC12) were greatly accelerated on the aligned oriented mats as compared to the randomly oriented mats during <I>in vitro</I> cell cultures. The morphology of the spontaneous outgrowth and phenotype of neurite bundles were preferentially guided along the axis of the aligned oriented nanofibers, which maintains a strong adaptability in axonal regeneration. In addition, the differentiation of PC12 cells cultured on as-fabricated NGCs were evaluated from cDNA gene expression. It is hoped that the results will contribute to the efficient application of designed NGCs and can be used in therapeutic strategies for treating injured sites and stimulate recovery from substantial damage to nerve cells.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>