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TNF-α 유전자로 형질전환된 효모의 성장양태 및 단백질 생산
심두희,이진옥,이동근,이재화 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-
사람 TNF-a를 생산하도록 GPD promoter와 AG promoter로 형질전환된 S. cerevisiae가 보이는 성장양상, 총분비단백질, pretease 활성을 측정하였다. GPD promoter로 형질전환 된yeast는 63.6 g/L의 성장이 일어났고 총분비단백질은 9 mg/L이며, pretease 활성은 2.55 X10^(4) unit/L 였다. AG promoter로 형질전환된 yeast의 경우엔 68 g/L의 성장이 측정되고 총분비단백질은 27 mg/L, pretease 활성1.55 X10^(4) unit/L였다. 단백질과 protease 분비량은 세포의 성장과 비례하는 것을 알 수 있었고, GPD promoter로 발현되는 TNF-a의 양은 배양초기에는 검출 한계 이하였고 15시간 이후에 증가하는 양상을 보여 배양 후 35시간째에 가장 많은 양 약 2535 ng/ml 정도가 분비되는 것으로 나타났다. We measured growth, total secreted protein and total secreted protease of transformed Saccharomyces cerevisiae with human TNF-α gene. Two different promoters, GPD promoter and AG promoter, were used for heterologous expression of human TNF-α gene. The growth which transformed TNF-α:GPD promoter was 63.9 g/L and total secreted protein was 9mg/L, protease activity was 2.55 x 10^(4) unit/L. In another case at TNF-α:AG promoter the growth was 68 g/L, total secreted protein was 27 mg/L and protease activity was 1.55 X 10^(4) unit/L. We could know total secreted protein and protease were directly proportional increase with growth. In initial time of culture, we could not see the TNF-α content in GPD promoter but after 15hours the yeast produced TNF-α. The highest level of product of TNF-a was 2535 ng/ml at 35hours after culture.
포름알데하이드에 폭로된 해부학 실습 학생들의 임파구 자매염색분체교환
백두진,주수자,김기영,이수진,심상효,손정일,심성한,송재철,조율희 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2
Sister-chromatid exchanges measured in the peripheral lymphocytes of 15 non-smoking medical students after exposure to formaldehyde during 24-week anatomy class showed a small but significant (p=0.0468) increase when compared with samples obtained from the same individuals immediately before exposure. Mean frequencies of sister-chromatid exchange of cultured peripheral lymphocytes were 5.40±0.24 from the samples before exposure and 5.87±0.22 from the same samples after exposure. Breathing-zone air samples collected by formaldehyde monitoring kit with digital colorimeter(SKC) showed a mean concentration of 0.72±0.02 ppm formaldehyde.
Shim, Sun Woo,Kwon, Doo Yeon,Lee, Bit Na,Kwon, Jin Seon,Park, Ji Hoon,Lee, Jun Hee,Kim, Jae Ho,Lee, Il Woo,Shin, Jung-Woog,Lee, Hai Bang,Kim, Wan-Doo,Kim, Moon Suk Mary Ann Liebert 2015 Tissue engineering. Part A Vol.21 No.5
<P>The present study employed nerve guidance conduits (NGCs) only, which were made of small intestine submucosa (SIS) and poly(caprolactone-co-lactide) (PCLA) to promote nerve regeneration in a peripheral nerve injury (PNI) model with nerve defects of 15?mm. The SIS- and PCLA-NGCs were easily prepared by rolling of a SIS sheet and a bioplotter using PCLA, respectively. The prepared SIS- and PCLA-NGCs fulfilled the general requirement for use as artificial peripheral NGCs such as easy fabrication, reproducibility for mass production, suturability, sterilizability, wettability, and proper mechanical properties to resist collapsing when applied to in vivo implantation. The SIS- and PCLA-NGCs appeared to be well integrated into the host sciatic nerve without causing dislocations and serious inflammation. All NGCs stably maintained their NGC shape for 8 weeks without collapsing, which matched well with the nerve regeneration rate. Staining of the NGCs in the longitudinal direction showed that the regenerated nerves grew successfully from the SIS- and PCLA-NGCs through the sciatic nerve-injured gap and connected from the proximal to distal direction along the NGC axis. SIS-NGCs exhibited a higher nerve regeneration rate than PCLA-NGCs. Collectively, our results indicate that SIS- and PCLA-NGCs induced nerve regeneration in a PNI model, a finding that has significant implications in the future with regard to the feasibility of clinical nerve regeneration with SIS- and PCLA-NGCs prepared through an easy fabrication method using promising biomaterials.</P>
상기도폐쇄 환자에 있어서 He-O2~혼합가스 흡입 후 류량기량곡선의 반응
심재두,김원동,강홍모,임선희,유지홍 대한내과학회 1986 대한내과학회지 Vol.31 No.3
In order to evaluate the ability of air and He-O2, maximal expiratory flow-volume curves to distinguish upper airway obstruction from peripheral airway obstruction of chronic obstructive pulmonary disease, the increase in expiratory flows at peak, 25, 50 and 75 per cent of forced vital capacity during He-O2 breathing compared to air breathing was determined in 10 normal control, 10 patients with chronic obstructive pulmonary disease and 10 patients with documented upper airway obstruction. 1) There was n significant difference in age and FVC, FEVFEU,/FVC and FEF 25 - 75% between the patients with chronic obstructive pulmonary disease and upper airway obstruction. 2) There was no significant difference in the increase of expiratory flows at 50 and 75 per cent of forced vital capacity between the patients with upper airway obstruction and normal controls. 3) There was significant difference in the in- crease of expiratory flows at 50 and 75 per cant of forced vital capacity between the patients with upper airway obstruction and chronic obstructive pulmonary disease. We conclude that upper airway obstruction can usually be identified by high helium responses and that upper airway obstruction can be identified even in the presence of more peripheral airway obstruction by a normal helium response at 50 and 75 percent of forced vital capacity.