가시오갈피 열매 추출물의 항돌연변이원성 및 세포독성 효과

전윤영,최승필,이효진,문선영,이득식,함승시 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

가시오갈피(Eleutherococcus senticous Maxim) 열매의 에탄올 추출물 및 분획물의 항돌연변이성 및 항암활성을 규명하였다. 항돌연별이원성 실험결과에서는 직접변이원인 MNNG, 4NQO 그리고 간접변이원인 B(α)P, Trp=P-1에 대해서 농도의존적인 돌연변이 억제활성을 나타내었다. MNNG (0.4㎍/plate)의 경우 S. typhimurium TA100 균주에서는 시료농도 200㎍/plate에서 클로로포름 분획물을 제외한 모든 분획물들에서 90%이상의 높은 억제효과를 나타내었다. 4NQO(0.15㎍/plate)에 대한 S. typhimurium TA98 균주의 경우 물 분획물의 경우 88.3%, TA100 균주에서는 에틸 아세테이트 분획물이 84.4%의 억제효과를 보였다. 간접변이원의 경우 B(α)P(10㎍/plate)에선느 에탄올 추출물과 핵산 분획물은 시료농도 200㎍/plate에서 각각 96.1%와 97.5%로 가장 높은 억제효과를 나타내었다. 그리고 Trp-P-1(0.15㎍/plate)에서는 TA100 균주의 경우 200㎍/plate 농도에서 에탄올 추출물과 물 분획물이 각각 95.5%와 90%로 높은 억제율을 보여주었고, TA98 균주에 대해서는 에틸아세티에트 분획물이 88.3%로 비교적 높은 억제효과를 나타내었다. 인간의 암세포인 폐암세포(A549), 위암세포(AGS), 간암세포(Hep3B), 유방암세포(MCF-7)에 대한 세포독성 억제 효과를 검토한 결과 모든 암세포에서 각분획물들은 1㎎/mL 농도에서 60% 이상의 비교적 높은 암세포 성장 효과를 나타내었다. 특히 MCF-7에서 높은 암세포 성장억제 효과를 나타내었는데 핵산 분획물(1㎎/mL)에서 92.7%의 높은 암세포 성장 억제 효과를 나타내었고 Hep3B에서는 부탄을 분획물(1㎎/mL)에서 82%의 비교적 높은 성장 억제효과를 나타내었다. This study was carried out to investigate the antimutagenic and cytotoxic effects of Eleutherococcus senticosus Maxim fruits ethanol extract on Salmonella typhimurium TA98, TA 100 and cancer cell lines using Ames test and SRB assay, respectively. They were extracted with ethanol and then fractionated with hexane, chloroform, ethyl acetate, butanol and water to get active fractions. In the Ames test, most of the extract had strong antimutagenic effects against the mutagenesis induced by MNNG, 4NQO, B(α)P Trp-P-1. The ethanol extract (200 ㎍/plate) of Eleutherococcus senticosus fruits showed 87.2% inhibitory effect on the mutagenesis induced by MNNG against TA100. And also, The suppression ratio against B(α)P and AGS, MCF-7, Hep3B), the value value of inhibition were mostly above 60% for each fraction (1 ㎎/mL). Hexane fraction (1 ㎎/mL) against showed the strongest cytotoxic effects of 92.7% compared to those of other fraction and butano fraetion against Hep3B was relatively high growth inhibitory effect of 82%.

Green, Energy-efficient, Health and Comfort, the New Trend of Northeast Rural Residence - Taking Hai Gou Village, Acheng District, Harbin as an example -

Sun Shi jun,Zhao Yun Duo 국제온돌학회 2012 International Journal of Ondol Vol.2012 No.-

With the rapid development of the rural economy and vigorous promotion of energy-saving technologies, it is found through a field investigation that the new rural housing in the northeast of China shows new changes on green, energy-efficiency, health, comfort and beauty. The new rural housing mainly applied the technologies of composite energy-saving wall, double or three layers of glass energy saving windows and the roof benzene board insulation,heated in winter by Kang with the fuel of straw, corn stalks and other bio energy, which brings prominent energy-saving effect and the improved indoor environment significantly. The only or main function of the Kang is no longer the heating facilities in winter, but to play its unique benefits of comfort and health for sleep as a bed. the highlights of cultural traditions and customs in Cold rural area, the changes of the Kang are smaller and more exquisite. In the future, northeast rural housing should integrate the technolgies of residential structures, materials and kang further to promote rural sustainable development of society, economy.

The Promotion of Healthy Energy-Efficient New Rural Housing - The rural residential investigation and analysis in Mayan Township, Shangzhi City, Heilongjiang Province

Sun Shi jun 국제온돌학회 2014 International Journal of Ondol Vol.2014 No.-

The Applications and Energy Saving Analysis of Straw Board Buildings in Cold Rural Regions of China - Case of Buildings in Harbin Suburb -

Sun Shi jun,Zhao Yun duo,Luan Ying Tao 국제온돌학회 2013 International Journal of Ondol Vol.2013 No.-

Straw board is renewable natural building material with the advantages of green environmental protection, strong applicability. But it hadn’t been promoted because of many reasons such as technology and economy. Through the analysis of the unique advantages and the recent new cases of straw board buildings in Harbin, and the current obstacles to application, The effective ways and the feasible solutions to promote sustainable straw board buildings are summarized.

The energy conservation analysis and application on HS-EPS core column building systems

Sun Shi jun,Yang yong,Lin Guohai 국제온돌학회 2011 International Journal of Ondol Vol.2011 No.-

this paper introduces An outline of the HS-EPS module building energy saving system of reinforced concrete core columns, its composition, applications and the technical specifications. A comprehensive analysis of the system on technical features and advantages of the system, such as building energy conservation, seismic, industrialization and standardization is introduced. In recent years this system has been applied in many projects in various parts of China including the the application and promotion in the post-disaster reconstruction projects. The ong-term study, extensive analysis and application of this system have proved its high performance, reliable quality and high application value.

A Study of Sustainable Development on Rural Ondol Residence in the Northeast of China

Shi Jun Sun,Yong Yang,Yun Duo Zhao 국제온돌학회 2006 International Journal of Ondol Vol.1 No.1

As we all know Ondol has been the most efficient and comfortable heating system of the rural residence in the Northeast of China for hundreds of years. But recently, through field survey, it was found that some country people replace the Ondol with homemade heating blindly. The result of this replacement is that heating effect of residence in winter is worse. And at the same time, it aggravates air pollution and increases heating payout. According to our real situation of energy sources reserve, environmental pollution and economic income of rural people, it is not only inpractical but also unreasonable, that in the northeast of China Ondol is replaced by homemade heating completely in winter. Ondol has its unique heating and hygienic efficacy. And it stands for cultural tradition and life custom of the country's cold areas. So we should improve Ondol combining resident structure and material. And it can make the Ondol system flourish with vigor and promote sustainable development of the country's traditional Ondol residence.

The practical technology research and application on rural energy saving Houses in Heilongjiang Province

Sun shi jun,Zhao Yun duo,Wang Li 국제온돌학회 2010 International Journal of Ondol Vol.2010 No.-

In order to achieve the goal of new rural construction in Heilongjiang province, national building energy-saving and emission reduction requirements, Harbin Institute of technology and Heilongjiang provincial research organizations cooperated for many years and finished this technological research project. the achievements include rural average heating energy consumption survey, rural energy-saving residential design suitable for different levels of consumption , detail structures, first applying of energy rural residential ventilation ,the research and development of new industrialized energy efficiency residence, rural, as well as releasing the Heilongjiang rural energy conservation housing construction technology guide. Passed a number of practical engineering inspections, with the significant environmental, economic and social benefits, widespread praising, this issue results won the 2010 Heilongjiang province science and technology progress second prize.

Tomato ( <i>Solanum lycopersicum</i> ) variety discrimination and hybridization analysis based on the 5S rRNA region

Sun, Yan-Lin,Kang, Ho-Min,Kim, Young-Sik,Baek, Jun-Pill,Zheng, Shi-Lin,Xiang, Jin-Jun,Hong, Soon-Kwan TaylorFrancis 2014 Biotechnology, biotechnological equipment Vol.28 No.3

<P>The tomato (<I>Solanum lycopersicum</I>) is a major vegetable crop worldwide. To satisfy popular demand, more than 500 tomato varieties have been bred. However, a clear variety identification has not been found. Thorough understanding of the phylogenetic relationship and hybridization information of tomato varieties is very important for further variety breeding. Thus, in this study, we collected 26 tomato varieties and attempted to distinguish them based on the 5S rRNA region, which is widely used in the determination of phylogenetic relations. Sequence analysis of the 5S rRNA region suggested that a large number of nucleotide variations exist among tomato varieties. These variable nucleotide sites were also informative regarding hybridization. Chromas sequencing of Yellow Mountain View and Seuwiteuking varieties indicated three and one variable nucleotide sites in the non-transcribed spacer (NTS) of the 5S rRNA region showing hybridization, respectively. Based on a phylogenetic tree constructed using the 5S rRNA sequences, we observed that 16 tomato varieties were divided into three groups at 95% similarity. Rubiking and Sseommeoking, Lang Selection Procedure and Seuwiteuking, and Acorn Gold and Yellow Mountain View exhibited very high identity with their partners. This work will aid variety authentication and provides a basis for further tomato variety breeding.</P>

review on the research of mechanical problems with different moduli in tension and compression

Jun-yi Sun,Hai-qiao Zhu,Shi-hong Qin,Da-lin Yang,Xiao-ting He 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.9

Most materials exhibit different tensile and compressive strains given the same stress applied in tension or compression. These materials are known as bimodular materials. An important model of bimodular materials is the criterion of positive-negative signs of principal stress proposed by Ambartsumyan. This model is mainly applicable to isotropic materials and deals with the principal stress state in a point. However, due to the inherent complexity of the constitutive relation, FEM based on iterative strategy and analytical methods based on a simplified mechanical model are required. In this paper, we review the basic assumptions of this model and its development, several innovative computational methods, and some important engineering applications. We also discuss the sequent key problems in this field.

Construction of a resequencing-based high-density genetic map for grape using an interspecific population (Vitis amurensis × Vitis vinifera)

Shi Guangli,Sun Dan,Wang Zhenxing,Liu Xiaoying,Guo Jianhui,Zhang Susu,Zhao Ying,Ai Jun 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.4

A high-density genetic map is a prerequisite for marker-assisted selection of crop species. Here, we developed a high-density genetic map of Vitis using 92 F1 progeny derived from the cross between two wine grape cultivars, ‘Shuanghong’ and ‘Italian Riesling’. Whole genome resequencing (WGS) technology was used to generate SNP markers for the map. The map consisted of 1351 recombination bin markers (210,873 SNPs), distributed on 19 linkage groups (LGs), with an average distance of 0.65 cM between adjacent bin markers and a map distance of 860.46 cM. The high levels of uniform coverage and collinearity between the 19 LGs and the grape reference genome indicated that the map is of high quality and can serve as a powerful platform for evaluating grapevines and deciphering the genetic mechanism of important economic and agronomic traits. Our findings will facilitate the process of mapping quantitative trait loci (QTL) that control important horticultural and economic traits of grape.