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Effective Analysis of Lung Infection using Fuzzy Rules
Sharad Kumar Tiwari,Navneet Walia,Harsukhpreet Singh,Anurag Sharma 보안공학연구지원센터 2015 International Journal of Bio-Science and Bio-Techn Vol.7 No.6
Soft Computing is a consortium of methodologies which works synergistically and provides an ability to make decision from reliable data or expert’s experience. Nowadays different types of soft computing techniques such as neural network, fuzzy logic, genetic algorithm and hybrid system are largely used in medical areas. In this paper, algorithm for analysis of lung infection is presented. The main focus is to develop system architecture to find probable disease stage patient may have. Severity level of disease is determined by using rule base method. The algorithm uses output of Rulebase and entered by user to determine level of infection.
Niraj Tripathi,Sharad Tiwari,Navinder Saini 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
In recent years, Coleus forskohlii has been considered plant as an important medicinal. Because of the continuous collection ofroots from the wild sources, this plant has been included in the list of endangered species. This has necessitated the use of biotechnologyin conservation and sustainable management of this endangered plant species. Morphological and molecular characterization ofthis herb will enhance our understanding in improving the optimal yields of Forskolin through breeding. To assess the morphologicaland molecular genetic diversity in 18 C. forskohlii genotypes collected from different places of central India, RAPD, ISSR, andAFLP marker systems were employed. Eleven RAPD, ten ISSRs and eight AFLP primers produced 101, 80, and 483 fragments,respectively. Among the three marker system used in this study, RAPD and ISSR showed 61.39 and 68.75% polymorphism, respectively,while eight AFLP primer combinations produced 70.81% polymorphism. UPGMA cluster analysis method group genotypesin two clusters with all marker systems separately and after combined analysis. Results show that both morphological and molecularfactors are effective in observing variations. Our results also indicate that the RAPD, ISSR, and AFLP approaches, along with pharmaceuticallyimportant morphological trait analysis, seemed to be best-suited for assessing the genetic relationships among distinctC. Forskohlii genotypes with high accuracy.
Tripathi, Niraj,Saini, Navinder,Tiwari, Sharad 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
In recent years, Coleus forskohlii has been considered plant as an important medicinal. Because of the continuous collection of roots from the wild sources, this plant has been included in the list of endangered species. This has necessitated the use of biotechnology in conservation and sustainable management of this endangered plant species. Morphological and molecular characterization of this herb will enhance our understanding in improving the optimal yields of Forskolin through breeding. To assess the morphological and molecular genetic diversity in 18 C. forskohlii genotypes collected from different places of central India, RAPD, ISSR, and AFLP marker systems were employed. Eleven RAPD, ten ISSRs and eight AFLP primers produced 101, 80, and 483 fragments, respectively. Among the three marker system used in this study, RAPD and ISSR showed 61.39 and 68.75% polymorphism, respectively, while eight AFLP primer combinations produced 70.81% polymorphism. UPGMA cluster analysis method group genotypes in two clusters with all marker systems separately and after combined analysis. Results show that both morphological and molecular factors are effective in observing variations. Our results also indicate that the RAPD, ISSR, and AFLP approaches, along with pharmaceutically important morphological trait analysis, seemed to be best-suited for assessing the genetic relationships among distinct C. Forskohlii genotypes with high accuracy.
Pharmacognostical Evaluation and Phytochemical Standardization of Abrus precatorius L. Seeds
Durgesh Verma,Shashi Shankar Tiwari,Sharad Srivastava,AKS Rawat 한국생약학회 2011 Natural Product Sciences Vol.17 No.1
The seeds of Abrus precatorius L. (Family- Fabaceae) constitute the drugs Abrus, Gunja, or Ratti in commerce. In the Indian System of Medicine, the seeds are used for sciatica, paralysis, headache, dysentery, diarrhoea, leprosy, ulcer, nervous disorders, alopecia, as well as anti-inflammatory, antidiabetic, antibacterial, antitumor, sexual stimulant and abortifacient. Seeds are poisonous and therefore are used after mitigation. The protein abrin is responsible for the highly toxic properties of seeds. Quantitative HPTLC analysis of the methanolic extract of seeds determined the presence of 0.4018% gallic acid and 0.4009% glycyrrhizin. The present study was undertaken to develop an HPTLC method, as well as ascertain the physico-chemical, morphological and histological parameters to establish the authenticity of A. precatorius seeds.