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RISS 인기검색어
- 검색키워드
- 저자 : Shaju Jacob Pulikkotil (검색결과 4 건)
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Shaju Pulikkotil Jacob,Sonia Nath,Ritu Prabha Patel 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.3
Purpose: Smokeless tobacco-based oral-use products like gutka are popular in India. Gutkausage leads to increased periodontal destruction and inflammation; however, the relevantmechanism remains unknown. This study aimed to elucidate the role of gutka in periodontitisby examining its effect on the levels of interleukin (IL) 1β and IL-8 from the gingivalcrevicular fluid (GCF). Methods: A total of 45 patients were enrolled in this study. Thirty patients with periodontitis(15 gutka chewers [GCP] and 15 nongutka chewers [NGC]) and 15 periodontallyhealthy controls (HC) were selected. The full-mouth plaque index (PI), gingival index (GI),probing depth (PD), clinical attachment level (CAL), and recession (RC) were recorded. TheIL-1β and IL-8 levels in the GCF of all subjects were assessed through an enzyme-linked immunosorbentassay (Quantikine). Results: The IL-1β and IL-8 levels were not significantly higher in the GCP group (IL-1β,369.01±273.44 μL; IL-8, 205.97±196.78 μL) as compared to those in the NGC group (IL-1β,195.57±96.85 μL; IL-8, 178.61±149.35 μL). More gingival RC and loss of attachment wasseen among the GCP group (RC: 2.02±0.31, P=0.013; CAL: 4.60±0.56, P<0.001) thanamong the NGC group (RC, 1.21±1.15; CAL, 3.70±0.32); however, PD was deeper amongthe NGC subjects (P=0.002). PI and GI were significantly higher for the periodontitis group(P<0.001) when compared to the HC, but there was no difference among gutka chewersand non-chewers (P=0.22 and P=0.89). A positive correlation was found between the IL-8levels and the duration of gutka chewing (r=–0.64, P<0.01). Conclusions: Gutka chewing leads to increased gingival RC and clinical loss of attachment. There was no effect seen in the proinflammatory cytokine levels in the GCF of gutka users.
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Chum, Jia Da,Lim, Darryl Jun Zhi,Sheriff, Sultan Omer,Pulikkotil, Shaju Jacob,Suresh, Anand,Davamani, Fabian The Korean Academy of Conservative Dentistry 2019 Restorative Dentistry & Endodontics Vol.44 No.1
Objectives: Irrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidis (S. epidermidis) for root canal disinfection. Materials and Methods: The minimum inhibitory concentration (MIC) was obtained using serial dilution method. The agar diffusion method was then used to determine the zones of inhibition for each irrigant. Lastly, forty 6-mm dentin blocks were prepared from human mandibular premolars and inoculated with S. epidermidis. Samples were randomly divided into 4 groups of 10 blocks and irrigated for 3 minutes with saline (control), 2% CHX, 3% NaOCl, or 0.1% OCT. Dentin samples were then collected immediately for microbial analysis, including an analysis of colony-forming units (CFUs). Results: The MICs of each tested irrigant were 0.05% for CHX, 0.25% for NaOCl, and 0.0125% for OCT. All tested irrigants showed concentration-dependent increase in zones of inhibition, and 3% NaOCl showed the largest zone of inhibition amongst all tested irrigants (p < 0.05). There were no significant differences among the CFU measurements of 2% CHX, 3% NaOCl, and 0.1% OCT showing complete elimination of S. epidermidis in all samples. Conclusions: This study showed that OCT was comparable to or even more effective than CHX and NaOCl, demonstrating antimicrobial activity at low concentrations against S. epidermidis.
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Jia Da Chum,Darryl Jun Zhi Lim,Sultan Omer Sheriff,Shaju Jacob Pulikkotil,Anand Suresh,Fabian Davamani 대한치과보존학회 2019 Restorative Dentistry & Endodontics Vol.44 No.1
Objectives: Irrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidis ( S. epidermidis ) for root canal disinfection. Materials and Methods: The minimum inhibitory concentration (MIC) was obtained using serial dilution method. The agar diffusion method was then used to determine the zones of inhibition for each irrigant. Lastly, forty 6-mm dentin blocks were prepared from human mandibular premolars and inoculated with S. epidermidis . Samples were randomly divided into 4 groups of 10 blocks and irrigated for 3 minutes with saline (control), 2% CHX, 3% NaOCl, or 0.1% OCT. Dentin samples were then collected immediately for microbial analysis, including an analysis of colony-forming units (CFUs). Results: The MICs of each tested irrigant were 0.05% for CHX, 0.25% for NaOCl, and 0.0125% for OCT. All tested irrigants showed concentration-dependent increase in zones of inhibition, and 3% NaOCl showed the largest zone of inhibition amongst all tested irrigants ( p < 0.05). There were no significant differences among the CFU measurements of 2% CHX, 3% NaOCl, and 0.1% OCT showing complete elimination of S. epidermidis in all samples. Conclusions: This study showed that OCT was comparable to or even more effective than CHX and NaOCl, demonstrating antimicrobial activity at low concentrations against S. epidermidis .
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