UBE2Q1 in a Human Breast Carcinoma Cell Line: Overexpression and Interaction with p53

Shafiee, Sayed Mohammad,Rasti, Mozhgan,Seghatoleslam, Atefeh,Azimi, Tayebeh,Owji, Ali Akbar Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

The p53 tumor suppressor protein is a principal mediator of growth arrest, senescence, and apoptosis in response to a broad array of cellular damage. p53 is a substrate for the ubiquitin-proteasome system, however, the ubiquitin-conjugating enzymes (E2s) involved in p53 ubiquitination have not been well studied. UBE2Q1 is a novel E2 ubiquitin conjugating enzyme gene. Here, we investigated the effect of UBE2Q1 overexpression on the level of p53 in the MDA-MB-468 breast cancer cell line as well as the interaction between UBE2Q1 and p53. By using a lipofection method, the p53 mutated breast cancer cell line, MDA-MB-468, was transfected with the vector pCMV6-AN-GFP, containing UBE2Q1 ORF. Western blot analysis was employed to verify the overexpression of UBE2Q1 in MDA-MB-468 cells and to evaluate the expression level of p53 before and after cell transfection. Immunoprecipitation and GST pull-down protocols were used to investigate the binding of UBE2Q1 to p53. We established MDA-MB-468 cells that transiently expressed a GFP fusion proteins containing UBE2Q1 (GFP-UBE2Q1). Western blot analysis revealed that levels of p53 were markedly lower in UBE2Q1 transfected MDA-MB-468 cells as compared with control MDA-MB-468 cells. Both in vivo and in vitro data showed that UBE2Q1 co-precipitated with p53 protein. Our data for the first time showed that overexpression of UBE2Q1can lead to the repression of p53 in MDA-MB-468 cells. This repression of p53 may be due to its UBE2Q1 mediated ubiquitination and subsequent proteasome degradation, a process that may involve direct interaction of UBE2Q1with p53.

Optimal burn-in and warranty for a product with post-warranty failure penalty

Shafiee, Mahmood,Chukova, Stefanka,Yun, Won Young Springer-Verlag 2014 INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TE Vol.70 No.1

Dynamic behavior of a functionally graded plate resting on Winkler elastic foundation and in contact with fluid

Shafiee, Ali A.,Daneshmand, Farhang,Askari, Ehsan,Mahzoon, Mojtaba Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.50 No.1

A semi-analytical method is developed to consider free vibrations of a functionally graded elastic plate resting on Winkler elastic foundation and in contact with a quiescent fluid. Material properties are assumed to be graded distribution along the thickness direction according to a power-law in terms of the volume fractions of the constituents. The fluid is considered to be incompressible and inviscid. In the analysis, the effect of an in-plane force in the plate due to the weight of the fluid is taken into account. By satisfying the compatibility conditions along the interface of fluid and plate, the fluid-structure interaction is taken into account and natural frequencies and mode shapes of the coupled system are acquired by employing energy methods. The results obtained from the present approach are verified by those from a finite element analysis. Besides, the effects of volume fractions of functionally graded materials, Winkler foundation stiffness and in-plane forces on the dynamic of plate are elucidated.

Development and Microstructural Characterization of a New Wrought High Entropy Superalloy

Ahad Shafiee,Mahmoud Nili‑Ahmadabadi,Hyoung Seop Kim,Mohammad Jahazi 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.5

In this study, a newly developed γ′ precipitation hardened high entropy superalloy (designated as HES-A1 hereafter) wasproduced and its microstructural features were characterized. The new alloy composition is based on the major elementsof conventional IN718 alloy, however other elements were added or removed to minimize possible presence of δ (or γʺ)phase and increase the volume fraction of very fine γ′ precipitates instead. Broadening the hot working window, lowerdensity, and avoiding the use of expensive elements were other considerations taken into account in the design of the newalloy. CALPHAD (CALculation of PHAse Diagrams) and PhaComp methods were used for the prediction of phases andtheir evolutions. The microstructure of the HES-A1 alloy in as-cast, homogenized, hot rolled, annealed and aged conditionswere characterized using optical, scanning, and transmission electron microscopes as well as X-ray diffraction technique. The as-cast microstructure of HES-A1 contained 5.5% of Laves phase, which was reduced to less than 0.3% through thedevelopment of a homogenization treatment. Hot rolling with reductions up to 36% at 1015 °C did not produce any cracking,indicating a good forming potential for the new alloy. The application of double aging treatment, similar to the one forIN718 alloy, showed no presence of γʺ or formation of δ phase in the microstructure. HES-A1 has been designed with ahigher Al/(Ti + Nb) ratio and higher proportion of Al + Ti + Nb so that it could be mainly strengthened by γ′ precipitation. The average size of monomodal γ′ precipitates was smaller than that observed in conventional alloys after similar treatments.

The Art of Captivating Writing: Exploring Digital Storytelling from a New Dimension

Hanieh Shafiee Rad 아시아테플 2024 The Journal of Asia TEFL Vol.21 No.1

While previous research has primarily focused on evaluating the effectiveness of digital storytelling (DST) itself, there is a dearth of knowledge regarding the impacts of flow, group engagement, and perceived instructional support on learners' DST experiences. Addressing this gap, the present study examines data collected from a sample of 225 students enrolled in DST-based writing courses. The results of this study indicate that learners' perceived instructional support significantly and positively influences their writing skills. Furthermore, the findings demonstrate that the relationship between instructional support and learning engagement is mediated by flow and group engagement. These findings underscore the significance of offering appropriate support to foster group engagement and facilitate students' flow experiences within the DST context. By shedding light on these less-explored aspects of the DST strategy, this study contributes to the existing literature in the field. It suggests that educators and instructional designers should consider the interplay of flow, group engagement, and instructional support when incorporating DST into their pedagogical practices. Understanding and effectively addressing these factors can enhance the learning experiences and outcomes of students engaged in DST-based writing activities. Further research is warranted to explore additional dimensions of DST and its impact on various educational contexts.

Effect of PCaRD DGB Flipped Learning on EFL Learners’ Grammar Skill

Hanieh Shafiee Rad 아시아영어교육학회 2021 The Journal of Asia TEFL Vol.18 No.2

This research examines the employing of a Play Curricular Activity Reflection Discussion (PCaRD) and Digital Game-based (DGB) through flipped learning on the EFL learners’ grammar skills to improve their achievement and attitudes. The participants were 66 EFL female intermediate students in Iran; the research was conducted over 10 weeks. A PCaRD DGB flipped learning was used for the experimental group (N = 33), and a standard flipped classroom was used for the control group (N = 33). Both groups constructed using the same teacher, time, coursebook, and videos. The PCaRD DGB flipped learning provided mobile-based game apps and a game-based feedback mechanism in the form of collaborative response and created a setting in which learners could engage in more grammar activity, pay more attention to their answers, and improving their grammar skills. The results indicated that the PCaRD DGB flipped learning outperformed the standard flipped learning in grammar achievement and in terms of their positive attitudes. The researcher speculates that the PCaRD DGB flipped learning strengthens EFL learners’ grammar skills and a positive attitude.

Repetitive transcranial magnetic stimulation: a potential therapeutic modality for chronic low back pain

( Sajad Shafiee ),( Farshad Hasanzadeh Kiabi ),( Misagh Shafizad ),( Amir Emami Zeydi ) 대한통증학회 2017 The Korean Journal of Pain Vol.30 No.1

Dynamic behavior of a functionally graded plate resting on Winkler elastic foundation and in contact with fluid

Ali A. Shafiee,Farhang Daneshmand,Ehsan Askari,Mojtaba Mahzoon 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.50 No.1

A semi-analytical method is developed to consider free vibrations of a functionally graded elastic plate resting on Winkler elastic foundation and in contact with a quiescent fluid. Material properties are assumed to be graded distribution along the thickness direction according to a power-law in terms of the volume fractions of the constituents. The fluid is considered to be incompressible and inviscid. In the analysis, the effect of an in-plane force in the plate due to the weight of the fluid is taken into account. By satisfying the compatibility conditions along the interface of fluid and plate, the fluid-structure interaction is taken into account and natural frequencies and mode shapes of the coupled system are acquired by employing energy methods. The results obtained from the present approach are verified by those from a finite element analysis. Besides, the effects of volume fractions of functionally graded materials, Winkler foundation stiffness and in-plane forces on the dynamic of plate are elucidated.

An evaluation of dietary adequacy among patients with constipation-predominant irritable bowel syndrome in Malaysia

( Nor Hamizah Shafiee ),( Nurul Huda Razalli ),( Norfilza M. Mokhtar ),( Eunice Tan ),( Raja Affendi Raja Ali ) 대한장연구학회 2022 Intestinal Research Vol.20 No.1

Background/Aims: Substantial proportions of patients with constipation-predominant irritable bowel syndrome (IBS-C) linked their symptoms with particular intake of foods. However, there is lack of current data regarding the intake among IBS-C patients before any dietary interventions. Thus, this study aimed to evaluate the dietary adequacy among IBS-C against the standard recommended nutrient intake (RNI) and healthy controls. Methods: A retrospective case-control study was conducted involving IBS-C patients and healthy control subjects. A validated 126-food items frequency questionnaire was administered to all the subjects to assess their dietary intake, guided by dietitians. The calculated nutrients intake for IBS-C patients was then compared against the standard RNI and healthy controls. Results: A total of 306 subjects were recruited, among which 218 were diagnosed with IBS-C and 88 were included as healthy controls. IBS-C patients had significantly lower intake of wholegrain products, fried foods, dairy products, fruits, and vegetables compared to healthy controls. The daily intake of energy, certain macronutrients, and micronutrients among IBS-C patients was significantly lower than the healthy subjects. Less than 5% of IBS-C patients and healthy subjects achieved the standard recommendation for dietary fiber. Also, various vitamin intake (B₁, B₂, B₆, folate, B₁₂, E, K, and potassium) among IBS-C patients did not meet the standard RNI. Conclusions: Dietary intakes of IBS-C patients did not meet the recommended intake for Malaysian and showed the nutritional inadequacies compared to the control subjects. Our study highlighted the importance of dietary evaluation prior to planning strategies for dietary intervention targeting IBS-C patients. (Intest Res 2022;20:124-133)

Anti-inflammatory diet and inflammatory bowel disease: what clinicians and patients should know?

( Nor Hamizah Shafiee ),( Zahara Abdul Manaf ),( Norfilza M. Mokhtar ),( Raja Affendi Raja Ali ) 대한장연구학회 2021 Intestinal Research Vol.19 No.2

Current treatment for inflammatory bowel disease (IBD) includes the application of anti-inflammatory agents for the induction and remission of IBD. However, prolonged use of anti-inflammatory agents can exert adverse effects on patients. Recently, formulated dietary approach in treating IBD patients is utilized to improve clinical activity scores. An alteration of gastrointestinal microbiota through dietary therapy was found to reduce IBD and is recognized as a promising therapeutic strategy for IBD. One of the recommended formulated diets is an anti-inflammatory diet (AID) that restricts the intake of carbohydrates with modified fatty acids. This diet also contains probiotics and prebiotics that can promote balanced intestinal microbiota composition. However, scientific evidences are limited to support this specific dietary regime in maintaining the remission and prevention relapse of IBD. Therefore, this review aimed to summarize available data from various studies to evaluate the AID diet effectiveness which will be useful for clinicians to manage their IBD patients by application of improved dietary therapy. (Intest Res 2021;19:171-185)