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      • 그루지아 민가의 전통 발효유에서 분리한 유산균의 이화학적 특성

        신승이,최기춘,김종현,박정수,이영환 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

        그루지아의 전통 발효유로부터 유산 생성과 항균성이 우수한 3주의 균주를 최종 분리, 선발하고 각각 Lactobacillus bulgaricus (L bulga G82), Lactobacillus acidophilus (L. acid G86), 그리고, Streptococcus thermophilus (S. thermo G90)로 동정하였다. 이들 유산균을 starter로 요구르트를 제조하여 이화학적 특성을 조사한 결과, pH는 4.0∼4.5의 범위를 보였으며, pH 4.0으로 L. acid G86 균주가 가장 낮게 나타났다. 또한 0.1N HCl에 대한 완충능은 4.50∼5.12ml, 0.1N NaOHdp 대한 완충능은 3.74∼4.98ml의 범위를 보였고, 3 균주 중 L acid G86 균주가 산-염기에 대하여 가장 높은 완충능을 나타내었다. 산도는 0.95∼1.16%, 점도는 1,984∼2,232 cps 그리고 생균수는 1.4x109∼3.0x109으로 조사 되었다. in vitro에서의 콜레스테롤 저하 정도는 L. acid G86 균주가 약 35%로 가장 높은 저하 정도를 보였고, L. bulga G82와 S. thermo G90 균주는 각각 약 32%, 24%이었다. Three strains of inhibitory lactic acid bacteria against pathogenic microorganism were isolated from traditional fermented milk of Georgia, and than, identified to be Lactobacillus bulgaricus (L bulga G82), Lactobacillus acidophilus (L acid G86) and Streptococcus thermophilus (S. thermo G90), respectively. These strains were used to starter for yogurts. So, we made yogurts, and evaluated their phyco-chemical properties. The range of pH value was 4.0∼4.5, and L. acid G86 strain was the lower pH value for 4,0. The buffer capacity against 0.1N HCI and 0.1N NaOH were 4,50~5.12ml and 3.74~4.98ml. The acidity, viscosity and viable cell counts of yogurt were 0.95∼1/16%, 1.984∼2,232 cps and 1.4x109∼3.0x109, respectively. Among the three strains, L. acid G86 strain was have the best ability of lowering cholesterol level in vitro, the value were about 35%. The L. bulga G82 and S. thermo G90 were around 32% and 24%, respectively.

      • Rhizopus oryzae KCTC 11970을 이용한 푸마르산 생산조건의 최적화

        김진남,양환승,이종일,류화원 全南大學校 觸媒硏究所 2002 觸媒硏究 論文集 Vol.23 No.-

        This work was developed in several steps to find the optimum conditions of fumaric acid production by Rhizopus oryzae KCTC 11970 in flask culture. Six different carbon sources and eleven nitrogen sources were tested. CaCO_3 was used as a neutralizing agent to control pH of medium. The optimum carbon source and nitrogen source were glucose 70 g/L and ammonium sulfate 0.4 g/L, respectively. At this optimum condition, the fumaric acid concentration, yield, and volumetric productivity were 19.33 g/L, 52.8%, and 0.32 g/L/h, respectivity, after fermentation time of 60 hr.

      • KCI등재

        Treatment of severe pain in a patient with complex regional pain syndrome undergoing dental treatment under general anesthesia: A case report

        Rhee, Seung-Hyun,Park, Sang-Hun,Ha, Sung-Ho,Ryoo, Seung-Hwa,Karm, Myong-Hwan,Kim, Hyun Jeong,Seo, Kwang-Suk The Korean Dental Society of Anesthsiology 2019 Journal of Dental Anesthesia and Pain Medicine Vol.19 No.5

        Complex regional pain syndrome (CRPS) is rare, characterized by pain from diverse causes, and presents as extreme pain even with minor irritation. General anesthesia may be required for dental treatment because the pain may not be controlled with local anesthesia. However, treatment under general anesthesia is also challenging. A 38-year-old woman with CRPS arrived for outpatient dental treatment under general anesthesia. At the fourth general anesthesia induction, she experienced severe pain resulting from her right toe touching the dental chair. Anesthesia was induced to calm her and continue the treatment. After 55 minutes of general anesthesia, the patient still complained of extreme toe pain. Subsequently, two administrations for intravenous sedation were performed, and discharge was possible in the recovery room approximately 5 h after the pain onset. The pain was not located at the dental treatment site. Although the major factor causing pain relief was unknown, ketamine may have played a role.

      • KCI등재

        Lipid emulsion therapy of local anesthetic systemic toxicity due to dental anesthesia

        Rhee, Seung-Hyun,Park, Sang-Hun,Ryoo, Seung-Hwa,Karm, Myong-Hwan The Korean Dental Society of Anesthsiology 2019 Journal of Dental Anesthesia and Pain Medicine Vol.19 No.4

        Local anesthetic systemic toxicity (LAST) refers to the complication affecting the central nervous system (CNS) and cardiovascular system (CVS) due to the overdose of local anesthesia. Its reported prevalence is 0.27/1000, and the representative symptoms range from dizziness to unconsciousness in the CNS and from arrhythmias to cardiac arrest in the CVS. Predisposing factors of LAST include extremes of age, pregnancy, renal disease, cardiac disease, hepatic dysfunction, and drug-associated factors. To prevent the LAST, it is necessary to recognize the risk factors for each patient, choose a safe drug and dose of local anesthesia, use vasoconstrictor, confirm aspiration and use incremental injection techniques. According to the treatment guidelines for LAST, immediate application of lipid emulsion plays an important role. Although lipid emulsion is commonly used for parenteral nutrition, it has recently been widely used as a non-specific antidote for various types of drug toxicity, such as LAST treatment. According to the recently published guidelines, 20% lipid emulsion is to be intravenously injected at 1.5 mL/kg. After bolus injection, 15 mL/kg/h of lipid emulsion is to be continuously injected for LAST. However, caution must be observed for >1000 mL of injection, which is the maximum dose. We reviewed the incidence, mechanism, prevention, and treatment guidelines, and a serious complication of LAST occurring due to dental anesthesia. Furthermore, we introduced lipid emulsion that has recently been in the spotlight as the therapeutic strategy for LAST.

      • 전술환경을 고려한 OSPF 적용방안 연구

        이승환(Seung Hwan Lee),신동욱(Dong Wook Shin),이승형(Seung Hyong Rhee) 한국정보과학회 2011 한국정보과학회 학술발표논문집 Vol.38 No.1D

        전술환경에서는 jamming과 같은 적의 공격과 노드의 잦은 이동에 의해 링크가 불안정 해질 수 있다. 유선환경을 고려한 상용 OSPF는 이러한 문제점에 대하여 적절하게 대응할 수 없는 단점이 있다. 기존의 OSPF는 jamming 신호가 발생하여 링크가 불안정해도 루트가 재설정되지 않는 단점을 가진다. 그리고 노드의 이동에 따른 control 정보의 전송으로 인해 네트워크의 통신 성능이 저하된다. 본 논문에서는 전술환경을 분석하고 기존의 상용 라우팅 프로토콜인 OSPF의 적용 방안에 대해서 알아보고 문제점을 보완할 수 있는 방안을 제안하였다.

      • KCI등재

        Matti-Russe 술식과 Fisk-Fernandez 술식을 이용한 주상골 불유합 치료에 관한 비교 연구

        이승환(Seung Hwan Rhee),정문상(Moon Sang Chung),이영호(Young Ho Lee),공현식(Hyun Sik Gong),김지영(Ji Yeong Kim),김민범(Min Boem Kim),오원석(Won Seok Oh),전숙하(Suk Ha Jeon),김정환(Jeong Hwan Kim),백구현(Goo Hyun Baek) 대한정형외과학회 2008 대한정형외과학회지 Vol.43 No.6

        목적: 확정형 주상골 불유합에 대하여 적절한 치료를 시행하지 않는 경우 수근골 불안정 및 골관절염의 진행을 피할 수 없는 것으로 알려져 있다. 본 연구에서는 Matti-Russe 술식과 Fisk-Fernandez 술식을 이용한 골유합술의 임상적 및 방사선학적 결과에 대해 비교, 고찰하고자 한다. 대상 및 방법: 2001년 9월부터 2006년 8월까지 본 병원에서 시행한 주상골 골유합술 증례 중 인터뷰에 응한 21례를 대상으로 하였고 평균 추시기간은 31개월이었다. 임상적 평가로는 관절운동범위, 파악력, 환자만족도, 동통 수준을 설문조사하고, 방사선학적 평가로는 골유합, 무혈성괴사, 골관절염 및 DISI 여부를 평가하였다. 또한, 두 수술군 사이에 결과의 차이가 있는지를 비교하였다. 결과: 두 수술군 사이에 동통이나 만족도의 유의한 차이는 없었다, 관절운동범위, 파악력 모두 건측에 비하여 감소한 소견을 보였으나 역시 두 수술군 사이에는 유의한 차이가 없었다. 두 수술군의 골유합률 및 수술 전후 DISI 교정각 사이에도 유의한 차이는 없었고 술 전 관찰된 DISI는 술 후 모두 교정된 소견을 보였다. 골관절염의 경우 전체적으로 7예에서 관찰되었는데, 그 중 술 전에 없던 골관절염이 새롭게 나타난 경우가 1예 있었다. 결론: 두 수술군 사이에 임상적, 방사선학적 평가에 있어서 통계적으로 유의한 차이를 보이지 않으며, 두 술식 모두 골유합과 기능적 회복에 있어서 효과적인 방법이다. Purpose: Established scaphoid nonunion may lead to carpal instability and osteoarthritis. The purpose of this study is to compare the clinical and radiological results of the osteosynthesis between Matti-Russe group and Fisk-Fernandez group. Materials and Methods: Our retrospective study included 21 nonunion cases in 20 patients treated by Matti-Russe or Fisk-Fernandez technique from September 2001 to August 2006. The mean duration of follow up was 31 months. Based on the questionnaire which included clinical information and the radiologic evaluation, we compared the objective and subjective results between two surgical groups. Results: There were no significant differences between two groups in respect of the postoperative satisfaction and the pain. Both the range of motion and the grip power decreased compared to that of the unaffected side, but there were no significant differences between two groups. Also, there were no significant differences in the union rate and the rate of successful correction of DISI deformity. In all cases in which DISI deformity was detected preoperatively, the deformity was corrected postoperatively. There were 7 cases of osteoarthritis, but among them only one case appeared newly after the surgery. Conclusion: There were no significant differences between two surgical groups in clinical and radiological results. Both techniques were effective in achieving bony union and functional recovery.

      • KCI등재
      • Detection of Kinase Activity Using Versatile Fluorescence Quencher Probes

        Rhee, Hyun-Woo,Lee, Seung Hwan,Shin, Ik-Soo,Choi, So Jung,Park, Hun Hee,Han, Kyungja,Park, Tai Hyun,Hong, Jong-In WILEY-VCH Verlag 2010 Angewandte Chemie Vol.122 No.29

        <B>Graphic Abstract</B> <P>Licht aus! Ein Mikrofluidiksystem für den Kinase-Nachweis verwendet fluoreszierende Peptide und eine phosphatselektive fluoreszenzlöschende Sonde (siehe Bild). Das System kann für die Echtzeitüberwachung von Kinasen, das Screening von Kinaseinhibitoren und, durch Detektion abnormer Kinaseaktivität in Patientenproben, für die Krebsdiagnose eingesetzt werden. <img src='wiley_img_2010/00448249-2010-122-29-ANGE201000879-content.gif' alt='wiley_img_2010/00448249-2010-122-29-ANGE201000879-content'> </P>

      • An Enhanced Scheme to Minimize a Packet Loss for Macro Handover in HMIPv6

        Seung Hwan Min,Sang Woo Son,Byung Ho Rhee 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7

        In order to support IP layer mobility management for IPv6 networks, Mobile IPv6 was emerged. But the MIPv6 has problems in a packet loss sensitive circumstance such as real-time services because It has a long time delay arised from registration process between MN and HA/CN. It causes the loss of data packets. Hence, HMIPv6(Hierarchical Mobile IPv6) appeared to solve the problems and support seamless mobility during Micro handover between ARs(Access Router) in the same MAP domain. But it has same problems in Macro handover between MAPs(Mobility Anchor Points) In this paper, we propose a enhanced scheme to minimize a CoA(Care of Address) registration delay and packet loss when the macro handover occur. It is achived through reducing a DAD(Duplicate Address Detection) latency and adding a packet forwarding process. In order to realize a packet forwarding, additional signaling between MAPs was required. The analytical result shows that the proposed scheme can reduce a packet loss time by up to 1/3 compared with original HMIPv6.

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