http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Setsuji Tone (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Production and release of pigments by plant hairy root cultures
Taya, Masahito,Tone, Setsuji 한국농화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.5
Recently, plant hairy roots have become of interest because of their active proliferation in a phytohormonefree medium and their capacity to produce valuable metabolites accumulated at comparable levels of the original plant roots. The purpose of our work is to extend the biotechnological application of hairy root cultures through studies on the following subjects. 1) Induction and characterization of hairy roots: Some hairy root clones were induced from red beet (Beta vulgaris), madder (Rubia tinctorum) and horseradish (Armoraeia lapathifolia) by Agrobacterium rhizogenes infection. These hairy roots had high contents of pigments or enzymes. 2) Bioreactor configuration: A turbine-blade fermentor was developed as a bioreactor suitable for the submerged culture of hiary root with a highly branched morphology. In this fermentor, a stainless steel mesh separated the culture space from the agitation space so that the hairy root cells were not in direct contact with the impellers. 3) Extracellular production of pigments: In the cultures of red beet and madder hairy roots, significant amounts of pigments were released from the cells into medium when the cells were subjected to the treatment of O₂ starvation. Both hairy roots were capable of growing even after the treatment. 4) Morphological change of hairy root: By cultivating the horseradish hairy root in the presence of phytohormones, the cell morphology could be changed reversibly between dedifferentiated-cell aggregates and organized hairy root.
-
Production and Release of Pigments by Plant Hairy Root Cultures
Masahito Taya,Setsuji Tone 한국응용생명화학회 1992 한국응용생명화학회 학술발표회 Vol.1992 No.-
Recently, plant hairy roots have become of interest because of their active proliferation in a phytohormonefree medium and their capacity to produce valuable metabolites accumulated at comparable levels of the original plant roots. The purpose of our work is to extend the biotechnological application of hairy root cultures through studies on the following subjects. 1) Induction and characterization of hairy roots: Some hairy root clones were induced from red beet (Beta vulgaris), madder (Rubia tinctorum) and horseradish (Armoraeia lapathifolia) by Agrobacterium rhizogenes infection. These hairy roots had high contents of pigments or enzymes. 2) Bioreactor configuration: A turbine-blade fermentor was developed as a bioreactor suitable for the submerged culture of hiary root with a highly branched morphology. In this fermentor, a stainless steel mesh separated the culture space from the agitation space so that the hairy root cells were not in direct contact with the impellers. 3) Extracellular production of pigments: In the cultures of red beet and madder hairy roots, significant amounts of pigments were released from the cells into medium when the cells were subjected to the treatment of O₂ starvation. Both hairy roots were capable of growing even after the treatment. 4) Morphological change of hairy root: By cultivating the horseradish hairy root in the presence of phytohormones, the cell morphology could be changed reversibly between dedifferentiated-cell aggregates and organized hairy root.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
