[Thyroid & Endocrinology] Anatomical Difference between Both Adrenal Tumors Could Be Helpful to Decide Appropriate Surgical Approach

Seonyeong Yang 대한외과학회 2021 대한외과학회 학술대회 초록집 Vol.2021 No.11

Oxidative biodegradation of 4-chlorophenol by using recombinant monooxygenase cloned and overexpressed from <i>Arthrobacter chlorophenolicus</i> A6

Kang, Christina,Yang, Jun Won,Cho, Wooyoun,Kwak, Seonyeong,Park, Sungyoon,Lim, Yejee,Choe, Jae Wan,Kim, Han S. Elsevier 2017 Bioresource technology Vol.240 No.-

<P><B>Abstract</B></P> <P>In this study, <I>cphC-I</I> and <I>cphB,</I> encoding a putative two-component flavin-diffusible monooxygenase (TC-FDM) complex, were cloned from <I>Arthrobacter chlorophenolicus</I> A6. The corresponding enzymes were overexpressed to assess the feasibility of their utilization for the oxidative decomposition of 4-chlorophenol (4-CP). Soluble CphC-I was produced at a high level (∼50%), and subsequently purified. Since CphB was expressed in an insoluble form, a flavin reductase, Fre, cloned from <I>Escherichia coli</I> was used as an alternative reductase. CphC-I utilized cofactor FADH<SUB>2</SUB>, which was reduced by Fre for the hydroxylation of 4-CP. This recombinant enzyme complex exhibited a higher specific activity for the oxidation of 4-CP (45.34U/mg-protein) than that exhibited by CphC-I contained in cells (0.18U/mg-protein). The Michaelis-Menten kinetic parameters were determined as: <I>v<SUB>max</SUB> </I> =223.3μM·min<SUP>−1</SUP>, <I>K<SUB>M</SUB> </I> =249.4μM, and <I>k<SUB>cat</SUB>/K<SUB>M</SUB> </I> =0.052min<SUP>−1</SUP>·μM<SUP>−1</SUP>. These results could be useful for the development of a new biochemical remediation technique based on enzymatic agents catalyzing the degradation of phenolic contaminants.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Arthrobacter chlorophenolicus</I> A6 possesses DNA genes for 4-chlorophenol degradation. </LI> <LI> Soluble form CphC-I was expressed, whereas CphB expressed in an insoluble form. </LI> <LI> CphC-I exhibited high specific activity when coupled with a flavin reductase, Fre. </LI> <LI> <I>v</I> <SUB>max</SUB> =223.3μM·min<SUP>−1</SUP>, <I>K<SUB>M</SUB> </I> =249.4μM, and <I>k<SUB>cat</SUB>/K<SUB>M</SUB> </I> =0.052min<SUP>−1</SUP>·μM<SUP>−1</SUP>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Color change from yellow to orange-red after 4-CP degradation by CphC-I. CphC-I concentration from 0.1 to 25μM from left to right.</P> <P>[DISPLAY OMISSION]</P>

Effect of Education on Discriminability of Montreal Cognitive Assessment Compared to Mini-Mental State Examination

Kim Haeyoon,Yang Seonyeong,Park Jaesel,Kim Byeong Chae,Yu Kyung-Ho,Kang Yeonwook 대한치매학회 2023 Dementia and Neurocognitive Disorders Vol.22 No.2

Background and Purpose: The Montreal Cognitive Assessment (MoCA) has been known as a screening test for detecting mild cognitive impairment (MCI) better than Mini-Mental State Examination (MMSE). However, in previous domestic studies, no significant difference was found in the discriminability between MoCA and MMSE. Researchers have suggested that this might be because older Koreans are less educated than older Westerners. This study was conducted to examine the effect of education on the discriminability of MoCA compared to the MMSE. Methods: Participants were 123 cognitively normal elderly, 118 with vascular MCI, 108 with amnestic MCI, 121 with vascular dementia, and 113 with dementia of the Alzheimer’s type. The Korean-MoCA (K-MoCA) and Korean-MMSE (K-MMSE) were administered. Multiple regression analyses and receiver operating characteristic (ROC) curve analyses were performed. Results: In all participants, education significantly affected both K-MoCA and K-MMSE scores along with age. The effect of education was re-examined by subgroup analysis after dividing subjects according to the level of education. Effect of education on K-MoCA and K-MMSE was only shown in the group with <9 years of education. ROC curve analyses revealed that the discriminability of K-MoCA to differentiate between vascular MCI and normal elderly was significantly higher than that of K-MMSE. When re-examining subgroups divided by education level, however, this higher discriminability of K-MoCA disappeared in the group with <9 years of education. Conclusions: These results indicate no difference in discriminating cognitive deficits between K-MoCA and K-MMSE in Korean elderly with <9 years of education.

대학생의 부모화 돌봄 경험과 대인관계 유능성의 관계에서 지각된 불공정성과 공감능력의 매개효과 - 성별에 따른 다집단 분석 -

박선영(Park Seonyeong),양수진(Yang Sujin) 한국인간발달학회 2019 人間發達硏究 Vol.26 No.2

본 연구는 성인 진입기 대학생들을 대상으로 청소년기의 부모화 돌봄 경험과 대인관계 유능성의 관계에서 지각된 불공정성과 공감능력의 매개효과와 구조적 관계에서의 성별에 따른 차이를 살펴보았다. 이를 위해 대학생 325명(남: 149명, 여: 176명)을 대상으로 자기보고식 설문조사를 실시하였고, M-plus를 사용하여 구조방정식 및 다집단 분석을 진행하였다. 연구 결과, 가족을 돌보는 부모화 돌봄 경험과 대인관계 유능성의 관계에서 지각된 불공정성의 단순매개효과가 나타났으며, 지각된 불공정성과 공감능력의 이중매개효과가 나타났다. 한편, 성별에 따른 다집단 분석 결과, 부모화 돌봄 경험과 대인관계 유능성의 직접 경로(남성의 경우만 정적으로 유의미), 지각된 불공정성을 매개로 한 부모화 돌봄 경험과 대인관계 유능성의 간접 경로에서(여성의 경우만 완전매개) 두드러진 차이를 보였다. 본 연구의 결과는 부모화 돌봄 경험보다는 이에 따른 지각된 불공정성이 대인관계 능력의 발달에 부정적인 기제로 작용할 수 있음을 밝히고, 부모화 경험을 세부적으로 탐색할 필요성을 제시하였다. 특히 성별에 따른 상이한 발달 경로를 확인하여, 남녀의 사회화 과정에서 부모화 경험이 갖는 의미를 밝혔다는 점에서 의의가 있다. This study aimed to identify the relationship between caregiving experience in parentification (CEP) and interpersonal competence, with perceived unfairness in parentification and empathic ability as mediators. A multi-group structural equation approach was performed sequentially to investigate the moderating effects of gender. The data were collected from 325 undergraduate students and analyzed with structural equation modeling (SEM) using M-Plus 6.12. The mediating effects of perceived unfairness and empathic ability on the relationship between caregiving and interpersonal competence were statistically significant. A moderator analysis by gender revealed significantly different pathways. The male group had a positively significant direct path from CEP to interpersonal competence, while the female group did not. The indirect path from CEP to interpersonal competence was perfectly mediated by perceived unfairness for females only. These results showed that parentification can have varying impacts on social development, particularly when its sub-constructs are divided into objective (caregiving) and subjective experience (perceived unfairness) categories. We also discuss how gender-associated socialization practices moderate the pathways between parentification and interpersonal relationships.

Identification of the upstream 4-chlorophenol biodegradation pathway using a recombinant monooxygenase from <i>Arthrobacter chlorophenolicus</i> A6

Cho, Su Yeon,Kwean, Oh Sung,Yang, Jun Won,Cho, Wooyoun,Kwak, Seonyeong,Park, Sungyoon,Lim, Yejee,Kim, Han S. Elsevier 2017 Bioresource technology Vol.245 No.2

<P><B>Abstract</B></P> <P>This study aimed to clarify the initial 4-chlorophenol (4-CP) biodegradation pathway promoted by a two-component flavin-diffusible monooxygenase (TC-FDM) consisting of CphC-I and CphB contained in <I>Arthrobacter chlorophenolicus</I> A6 and the decomposition function of CphC-I. The TC-FDM genes were cloned from <I>A. chlorophenolicus</I> A6, and the corresponding enzymes were overexpressed. Since CphB was expressed in an insoluble form, Fre, a flavin reductase obtained from <I>Escherichia coli</I>, was used. These enzymes were purified using Ni<SUP>2+</SUP>-NTA resin. It was confirmed that TC-FDM catalyzes the oxidation of 4-CP and the sequential conversion of 4-CP to benzoquinone (BQN)→hydroquinone (HQN)→HQL. This indicated that CphC-I exhibits substrate specificity for 4-CP, BQN, and HQN. The activity of CphC-I for 4-CP was 63.22U/mg-protein, and the Michaelis-Menten kinetic parameters were <I>v<SUB>max</SUB> </I> =0.21mM/min, <I>K<SUB>M</SUB> </I> =0.19mM, and <I>k<SUB>cat</SUB> </I>/<I>K<SUB>M</SUB> </I> =0.04mM<SUP>−1</SUP> min<SUP>−1</SUP>. These results would be useful for the development of a novel biochemical treatment technology for 4-CP and phenolic hydrocarbons.</P> <P><B>Highlights</B></P> <P> <UL> <LI> TC-FDM catalyzes initial 4-chlorophenol biodegradation pathway. </LI> <LI> 4-CP is converted sequentially to BQN→HQN→HQL by CphC-I. </LI> <LI> The activity of CphC-I for 4-CP was 63.22U/mg-protein. </LI> <LI> <I>v<SUB>max</SUB> </I> =0.21mM/min, <I>K<SUB>M</SUB> </I> =0.19mM, and <I>k<SUB>cat</SUB> </I>/<I>K<SUB>M</SUB> </I> =0.04mM<SUP>−1</SUP> min<SUP>−1</SUP>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Immobilization of enzymes onto clay minerals for the biochemical decomposition of 4-chlorophenol

( Oh Oh Sung Kwean ),( Su Yeon Cho ),( Jun Won Yang ),( Wooyoun Cho ),( Seonyeong Kwak ),( Sungyoon Park ),( Yejee Lim ),( Han S. Kima ) 한국물환경학회 2017 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2017 No.-

In this study an oxidative enzyme was immobilized onto inorganic backbone materials to stimulate the detoxification of toxic aromatic hydrocarbon compounds. Smectite clay minerals and soil organic matter were screened as an enzyme support and a binding agent, respectively. Montmorillonite of which inner pores are layered with nano-scale spacing planar was activated by humic acid. A dioxygenase obtained by cloning of its corresponding gene from Arthrobacter chlorophenolicus A6 was immobilized onto the humic acid-activated montmorillonite. Oxygenated metabolites such as catechol and 4-chlorocatechol were selected as target aromatic contaminants (primary substrates of enzyme). The enzyme immobilization yield was as high as 63% and the reductions in enzyme activity for the decomposition of substrate compounds during enzyme immobilization were minimal: 15% for catechol and 24% for 4-chlorocatechol, respectively. The kinetic analysis of the free and immobilized enzymes demonstrated a slight decrease of vmax and a marginal increase of KM as compared with those for the free enzyme, indicating the changes in enzyme activity perhaps due to the changes in enzyme conformation associated with its immobilization were minimal. The results for the effects of environmental factors including pH, temperature, and ionic strength on the activity of free and immobilized enzymes showed that the activity of free enzyme changed significantly in response to the changes of the environmental factors whereas that of immobilized enzyme was pretty much consistent. This indicated that the stability of enzyme against the abrupt changes in environmental factors can be greatly improved by enzyme immobilization. The results of this study support the feasibility of a new environmental fusion technology based on bio-technology and nano-technology for the development of biochemical treatment processes.

Korean Version of Inventory of Complicated Grief Scale: Psychometric Properties in Korean Adolescents

Han, Doug Hyun,Lee, Jung Jae,Moon, Duk-Soo,Cha, Myoung-Jin,Kim, Min A,Min, Seonyeong,Yang, Ji Hoon,Lee, Eun Jeong,Yoo, Seo Koo,Chung, Un-Sun The Korean Academy of Medical Sciences 2016 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.31 No.1

<P>We aimed to validate the Inventory of Complicated Grief (ICG)-Korean version among 1,138 Korean adolescents, representing a response rate of 57% of 1,997 students. Participants completed a set of questionnaires including demographic variables (age, sex, years of education, experience of grief), the ICG, the Children's Depression Inventory (CDI) and the Lifetime Incidence of Traumatic Events-Child (LITE-C). Exploratory factor analysis was performed to determine whether the ICG items indicated complicated grief in Korean adolescents. The internal consistency of the ICG-Korean version was Cronbach's α=0.87. The test-retest reliability for a randomly selected sample of 314 participants in 2 weeks was <I>r</I>=0.75 (<I>P</I><0.001). Concurrent validity was assessed using a correlation between the ICG total scores and the CDI total scores (<I>r</I>=0.75, <I>P</I><0.001). The criterion-related validity based on the comparison of ICG total scores between adolescents without complicated grief (1.2±3.7) and adolescent with complicated grief (3.2±6.6) groups was relatively high (t=5.71, <I>P</I><0.001). The data acquired from the 1,138 students was acceptable for a factor analysis (Kaiser-Meyer-Olkin Measure of Sampling Adequacy=0.911; Bartlett's Test of Sphericity, χ<SUP>2</SUP>=13,144.7, <I>P</I><0.001). After omission of 3 items, the value of Cronbach's α increased from 0.87 for the 19-item ICG-Korean version to 0.93 for the 16-item ICG-Korean version. These results suggest that the ICG is a useful tool in assessing for complicated grief in Korean adolescents. However, the 16-item version of the ICG appeared to be more valid compared to the 19-item version of the ICG. We suggest that the 16-item version of the ICG be used to screen for complicated grief in Korean adolescents.</P>