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Jo, Seongjae,Kim, Insu,Lee, Wonseok,Kim, Minwoo,Park, Joohyung,Lee, Gyudo,Yoon, Dae Sung,Park, Jinsung Elsevier Applied Science 2019 Biosensors & bioelectronics Vol. No.
<P><B>Abstract</B></P> <P>Fibrinogen, which is a glycoprotein that circulates in the blood, plays various important biological roles, <I>e.g.,</I> in blood coagulation, fibroblast proliferation, angiogenesis, and wound healing. Abnormal levels of fibrinogen in plasma have been identified as a key biomarker of a variety of disorders from cardiovascular diseases to hemophilia. Therefore, the development of a quantitative assay for fibrinogen in the blood has emerged as an important issue for the prevention and diagnosis of these diseases. Meanwhile, it is well known that erythrocytes can selectively capture fibrinogen because of the fibrinogen receptor expressed on their plasma membrane. Inspired by these biological interactions, herein, we devised an erythrocyte membrane (EM)-blanketed biosensor based on localized surface plasmon resonance (LSPR) for highly sensitive detection of fibrinogen. By placing the EM onto a nanoparticle-on-substrate, we enhanced the LSPR signal, achieving highly sensitive and selective detection of fibrinogen. We demonstrated that fibrinogen detection is possible over a wide concentration range, 0.001–5.000 mg/mL, which can cover normal and pathological blood fibrinogen levels. In addition, it was verified that the biosensor selectively detects fibrinogen in comparison with other human-blood-plasma components. The nanoplasmonic sensor blanketed with the EM opens up new opportunities for the development of a robust fibrinogen-sensing technology.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The highly sensitive EM-blanketed LSPR biosensor for detection of fibrinogen has been developed. </LI> <LI> The coating EMs onto the sensor can improve the LSPR signal by stable attachment of AuNPs onto the sensor. </LI> <LI> The EM-blanketed LSPR sensor showed wide linear dynamic range and very low detection limit. </LI> <LI> The proposed sensor was used for determination of fibrinogen in human blood plasma samples. </LI> </UL> </P>
Seongjae Jo,Jinyeong Kim,Yejin Kim,Oh Seok Kwon 한국진공학회(ASCT) 2021 Applied Science and Convergence Technology Vol.30 No.6
Owing to rapid climate change and increasingly stringent carbon regulations, carbon dioxide detection is becoming more important. In this study, we fabricate a cucurbit[6]uril-functionalized gold nanorod-based localized surface plasmon resonance (LSPR) gas sensor to detect carbon dioxide. The gold nanorods provide a high refractive index unit that enables the measurement of gas molecules with low molecular weights, while cucurbit[6]uril is a chemical receptor that binds to carbon dioxide owing to its structural characteristics. Therefore, cucurbit[6]uril was functionalized through direct adhesion on the surface of gold nanorods, which was replaced with citrate. The manufactured sensor can detect the presence of carbon dioxide at a maximum concentration of 400 ppm in the atmosphere. The high potential applicability of the cucurbit[6]uril-applied LSPR gas sensors is demonstrated in this study.
국소 표면 플라즈몬 공명을 이용한 바이오센서의 생체물질 검출 동향
조성재(Seongjae Jo) 한국진공학회 2021 진공 이야기 Vol.8 No.3
This paper explains biosensors based on localized surface plasmon resonance (LSPR). The biosensor that detect biological materials include a boundless range for selecting object, specifying a target, and detecting technology. Among the biosensors of various sensing techniques, this paper demonstrates the principles of LSPR and how this technology applies to sensors. To generating the LSPR effect, a nano-sized metal structure must be designed. Recently, a very precise LSPR sensor can be produced through advanced method of metal nanoparticle synthesis and metal nanostructure fabrication. The current research trends show several biological materials, such as DNA, RNA, antibodies, enzymes, proteins, cells, biological membranes and hormones, were detected through LSPR sensors with different nanoplasmonics methods, respectively. This above mechanism optimized by combining various LSPR methods can provide new insight into diverse techniques applied with nanoplasmonics. Furthermore, the research results have the potential to develop into a commercialized LSPR biosensor through these achievements.